AU4224997A - Total synthesis and functional overexpression of a (candida rugosa) lip1 gene coding for a major industrial lipase - Google Patents

Total synthesis and functional overexpression of a (candida rugosa) lip1 gene coding for a major industrial lipase

Info

Publication number
AU4224997A
AU4224997A AU42249/97A AU4224997A AU4224997A AU 4224997 A AU4224997 A AU 4224997A AU 42249/97 A AU42249/97 A AU 42249/97A AU 4224997 A AU4224997 A AU 4224997A AU 4224997 A AU4224997 A AU 4224997A
Authority
AU
Australia
Prior art keywords
gene coding
total synthesis
candida rugosa
major industrial
lip1 gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU42249/97A
Other languages
English (en)
Inventor
Lilia Alberghina
Stefania Brocca
Marina Lotti
Rolf Schmid
Claudia Schmidt-Dannert
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Unilever NV
Original Assignee
Unilever NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unilever NV filed Critical Unilever NV
Publication of AU4224997A publication Critical patent/AU4224997A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU42249/97A 1997-09-16 1997-09-16 Total synthesis and functional overexpression of a (candida rugosa) lip1 gene coding for a major industrial lipase Abandoned AU4224997A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/NL1997/000524 WO1999014338A1 (fr) 1997-09-16 1997-09-16 Synthese totale et surexpression fonctionnelle d'un gene candida rugosa lip1 codant pour une lipase industrielle majeure

Publications (1)

Publication Number Publication Date
AU4224997A true AU4224997A (en) 1999-04-05

Family

ID=19866211

Family Applications (1)

Application Number Title Priority Date Filing Date
AU42249/97A Abandoned AU4224997A (en) 1997-09-16 1997-09-16 Total synthesis and functional overexpression of a (candida rugosa) lip1 gene coding for a major industrial lipase

Country Status (3)

Country Link
EP (1) EP1012301A1 (fr)
AU (1) AU4224997A (fr)
WO (1) WO1999014338A1 (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9907082D0 (en) * 1999-03-26 1999-05-19 Chirotech Technology Ltd The preparation of carboxylic acid derivatives
EP1130100A1 (fr) * 2000-02-14 2001-09-05 Unilever N.V. Enzymes lipolytiques modifiées et leur utilisation
US6706474B1 (en) 2000-06-27 2004-03-16 Board Of Trustees Of The University Of Illinois Nucleic acid enzyme biosensors for ions
DE10100913A1 (de) * 2001-01-11 2002-07-25 Haarmann & Reimer Gmbh Verfahren zur Herstellung von L-Menthol
CN100336906C (zh) * 2001-01-15 2007-09-12 广州市绿巨人生物环保技术有限公司 脂肪酶基因序列及其在酵母中的应用
US7052879B2 (en) 2001-08-31 2006-05-30 Academia Sinica Recombinant Candida rugosa lipases
DK2853593T3 (en) 2003-03-07 2018-01-08 Dsm Ip Assets Bv Hydrolases, nucleic acids encoding them, and processes for their preparation and use
US20080070291A1 (en) 2004-06-16 2008-03-20 David Lam Compositions and Methods for Enzymatic Decolorization of Chlorophyll
CN104498450B (zh) * 2014-12-18 2017-02-22 上海交通大学 褶皱假丝酵母脂肪酶1突变体及其基因
CA3025596A1 (fr) 2016-06-10 2017-12-14 Dsm Ip Assets B.V. Lipase mutante et utilisation de cette derniere
CN106047917B (zh) * 2016-07-25 2019-10-29 北京化工大学 产脂肪酶的毕赤酵母基因工程菌株的构建方法
WO2019219904A2 (fr) 2018-05-18 2019-11-21 Dsm Ip Assets B.V. Lipase mutante et utilisation de cette dernière
CA3100611A1 (fr) 2018-05-18 2019-11-21 Dsm Ip Assets B.V. Lipase mutante et utilisation associee

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2050068B1 (es) * 1992-07-03 1994-12-16 Consejo Superior Investigacion Procedimiento para la purificacion de dos isoenzimas lipasa de candida rugosa.

Also Published As

Publication number Publication date
WO1999014338A1 (fr) 1999-03-25
EP1012301A1 (fr) 2000-06-28

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