AU2903700A - Vaccine - Google Patents
Vaccine Download PDFInfo
- Publication number
- AU2903700A AU2903700A AU29037/00A AU2903700A AU2903700A AU 2903700 A AU2903700 A AU 2903700A AU 29037/00 A AU29037/00 A AU 29037/00A AU 2903700 A AU2903700 A AU 2903700A AU 2903700 A AU2903700 A AU 2903700A
- Authority
- AU
- Australia
- Prior art keywords
- polypeptide
- polynucleotide
- genes
- sequence
- seq
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 229960005486 vaccine Drugs 0.000 title claims description 23
- 108090000623 proteins and genes Proteins 0.000 claims description 194
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 146
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 141
- 229920001184 polypeptide Polymers 0.000 claims description 140
- 102000040430 polynucleotide Human genes 0.000 claims description 120
- 108091033319 polynucleotide Proteins 0.000 claims description 120
- 239000002157 polynucleotide Substances 0.000 claims description 119
- 230000007918 pathogenicity Effects 0.000 claims description 97
- 238000000034 method Methods 0.000 claims description 58
- 125000003729 nucleotide group Chemical group 0.000 claims description 54
- 239000002773 nucleotide Substances 0.000 claims description 53
- 239000012634 fragment Substances 0.000 claims description 49
- 230000001018 virulence Effects 0.000 claims description 45
- 241000588832 Bordetella pertussis Species 0.000 claims description 44
- 210000004027 cell Anatomy 0.000 claims description 35
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 32
- 230000014509 gene expression Effects 0.000 claims description 30
- 108010069584 Type III Secretion Systems Proteins 0.000 claims description 28
- 239000000203 mixture Substances 0.000 claims description 27
- 230000000295 complement effect Effects 0.000 claims description 25
- 230000001580 bacterial effect Effects 0.000 claims description 21
- 201000010099 disease Diseases 0.000 claims description 21
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 21
- 241000894006 Bacteria Species 0.000 claims description 20
- 239000000523 sample Substances 0.000 claims description 17
- 101150053156 lcrD gene Proteins 0.000 claims description 16
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 15
- 238000009396 hybridization Methods 0.000 claims description 14
- 241000607734 Yersinia <bacteria> Species 0.000 claims description 13
- 208000015181 infectious disease Diseases 0.000 claims description 12
- 230000001717 pathogenic effect Effects 0.000 claims description 12
- 241001465754 Metazoa Species 0.000 claims description 11
- 238000012163 sequencing technique Methods 0.000 claims description 11
- 238000012216 screening Methods 0.000 claims description 10
- 230000002163 immunogen Effects 0.000 claims description 9
- 238000004519 manufacturing process Methods 0.000 claims description 9
- 230000008569 process Effects 0.000 claims description 9
- 201000005702 Pertussis Diseases 0.000 claims description 8
- 230000028993 immune response Effects 0.000 claims description 8
- 239000000427 antigen Substances 0.000 claims description 7
- 108091007433 antigens Proteins 0.000 claims description 7
- 102000036639 antigens Human genes 0.000 claims description 7
- 241000282412 Homo Species 0.000 claims description 3
- 229940052491 bordetella pertussis Drugs 0.000 claims description 3
- 239000003814 drug Substances 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 244000005700 microbiome Species 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims description 2
- 230000001225 therapeutic effect Effects 0.000 claims description 2
- 239000003937 drug carrier Substances 0.000 claims 3
- 239000013604 expression vector Substances 0.000 claims 3
- 238000012258 culturing Methods 0.000 claims 2
- 239000012472 biological sample Substances 0.000 claims 1
- 210000001768 subcellular fraction Anatomy 0.000 claims 1
- 230000001131 transforming effect Effects 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 description 76
- 241000588807 Bordetella Species 0.000 description 67
- 108020004414 DNA Proteins 0.000 description 45
- 239000013615 primer Substances 0.000 description 33
- 239000012636 effector Substances 0.000 description 26
- 108700026244 Open Reading Frames Proteins 0.000 description 22
- 239000000047 product Substances 0.000 description 21
- 230000028327 secretion Effects 0.000 description 21
- 101100262843 Enterococcus faecalis uppP gene Proteins 0.000 description 20
- 101100437363 Thauera aromatica bcrD gene Proteins 0.000 description 20
- 238000003752 polymerase chain reaction Methods 0.000 description 19
- 241000894007 species Species 0.000 description 16
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 15
- 241000588724 Escherichia coli Species 0.000 description 15
- 150000001413 amino acids Chemical class 0.000 description 14
- 239000013598 vector Substances 0.000 description 14
- 238000004458 analytical method Methods 0.000 description 13
- 230000000694 effects Effects 0.000 description 13
- 239000013612 plasmid Substances 0.000 description 11
- 108010028773 Complement C5 Proteins 0.000 description 10
- 108091034117 Oligonucleotide Proteins 0.000 description 10
- 241000700605 Viruses Species 0.000 description 10
- 230000003321 amplification Effects 0.000 description 10
- 210000000349 chromosome Anatomy 0.000 description 10
- 238000009472 formulation Methods 0.000 description 10
- 238000003199 nucleic acid amplification method Methods 0.000 description 10
- 108091026890 Coding region Proteins 0.000 description 9
- 102000053602 DNA Human genes 0.000 description 9
- 230000004048 modification Effects 0.000 description 9
- 238000012986 modification Methods 0.000 description 9
- 101710175625 Maltose/maltodextrin-binding periplasmic protein Proteins 0.000 description 8
- 230000015572 biosynthetic process Effects 0.000 description 8
- 238000010367 cloning Methods 0.000 description 8
- 230000000875 corresponding effect Effects 0.000 description 8
- 108700039887 Essential Genes Proteins 0.000 description 7
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 7
- 230000004927 fusion Effects 0.000 description 7
- 238000003780 insertion Methods 0.000 description 7
- 230000037431 insertion Effects 0.000 description 7
- 101150066555 lacZ gene Proteins 0.000 description 7
- 108020004705 Codon Proteins 0.000 description 6
- 230000000890 antigenic effect Effects 0.000 description 6
- 239000002299 complementary DNA Substances 0.000 description 6
- 230000002068 genetic effect Effects 0.000 description 6
- 230000001900 immune effect Effects 0.000 description 6
- 108700005089 MHC Class I Genes Proteins 0.000 description 5
- 241000124008 Mammalia Species 0.000 description 5
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 5
- 238000007792 addition Methods 0.000 description 5
- 238000001042 affinity chromatography Methods 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 230000002759 chromosomal effect Effects 0.000 description 5
- 238000012217 deletion Methods 0.000 description 5
- 230000037430 deletion Effects 0.000 description 5
- 238000003745 diagnosis Methods 0.000 description 5
- 238000005755 formation reaction Methods 0.000 description 5
- 230000006870 function Effects 0.000 description 5
- 238000000746 purification Methods 0.000 description 5
- 230000003248 secreting effect Effects 0.000 description 5
- -1 that is Substances 0.000 description 5
- 230000014616 translation Effects 0.000 description 5
- 230000007923 virulence factor Effects 0.000 description 5
- 239000000304 virulence factor Substances 0.000 description 5
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 4
- 101100165660 Alternaria brassicicola bsc6 gene Proteins 0.000 description 4
- 108091093088 Amplicon Proteins 0.000 description 4
- 101100499295 Bacillus subtilis (strain 168) disA gene Proteins 0.000 description 4
- 241000590002 Helicobacter pylori Species 0.000 description 4
- 101150007210 ORF6 gene Proteins 0.000 description 4
- 101100226894 Phomopsis amygdali PaGT gene Proteins 0.000 description 4
- 238000003556 assay Methods 0.000 description 4
- 230000027455 binding Effects 0.000 description 4
- 230000004071 biological effect Effects 0.000 description 4
- 238000012512 characterization method Methods 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 230000000369 enteropathogenic effect Effects 0.000 description 4
- 108020001507 fusion proteins Proteins 0.000 description 4
- 102000037865 fusion proteins Human genes 0.000 description 4
- 229940037467 helicobacter pylori Drugs 0.000 description 4
- 210000004408 hybridoma Anatomy 0.000 description 4
- 238000001727 in vivo Methods 0.000 description 4
- 230000001965 increasing effect Effects 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 108020004999 messenger RNA Proteins 0.000 description 4
- 230000035772 mutation Effects 0.000 description 4
- 102000039446 nucleic acids Human genes 0.000 description 4
- 108020004707 nucleic acids Proteins 0.000 description 4
- 150000007523 nucleic acids Chemical class 0.000 description 4
- 230000036961 partial effect Effects 0.000 description 4
- 244000052769 pathogen Species 0.000 description 4
- 230000001323 posttranslational effect Effects 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 238000006467 substitution reaction Methods 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 238000013519 translation Methods 0.000 description 4
- 101100165658 Alternaria brassicicola bsc5 gene Proteins 0.000 description 3
- 101100032924 Bacillus subtilis (strain 168) radA gene Proteins 0.000 description 3
- 241000588779 Bordetella bronchiseptica Species 0.000 description 3
- 241000589969 Borreliella burgdorferi Species 0.000 description 3
- 241000010804 Caulobacter vibrioides Species 0.000 description 3
- 101100446735 Caulobacter vibrioides (strain ATCC 19089 / CB15) flhA gene Proteins 0.000 description 3
- 108020004635 Complementary DNA Proteins 0.000 description 3
- 238000001712 DNA sequencing Methods 0.000 description 3
- 101100001914 Danio rerio apmap gene Proteins 0.000 description 3
- 101100492392 Didymella fabae pksAC gene Proteins 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 108700005092 MHC Class II Genes Proteins 0.000 description 3
- 101100226897 Phomopsis amygdali PaAT-1 gene Proteins 0.000 description 3
- 101100226893 Phomopsis amygdali PaP450-2 gene Proteins 0.000 description 3
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 3
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 3
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 3
- 101100220583 Rhizobium meliloti (strain 1021) cheD gene Proteins 0.000 description 3
- 102000006382 Ribonucleases Human genes 0.000 description 3
- 108010083644 Ribonucleases Proteins 0.000 description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- 238000012300 Sequence Analysis Methods 0.000 description 3
- 101000870438 Streptococcus gordonii UDP-N-acetylglucosamine-peptide N-acetylglucosaminyltransferase stabilizing protein GtfB Proteins 0.000 description 3
- 101000645119 Vibrio campbellii (strain ATCC BAA-1116 / BB120) Nucleotide-binding protein VIBHAR_03667 Proteins 0.000 description 3
- 241000607447 Yersinia enterocolitica Species 0.000 description 3
- 230000002238 attenuated effect Effects 0.000 description 3
- 244000052616 bacterial pathogen Species 0.000 description 3
- 101150062295 bcrD gene Proteins 0.000 description 3
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 125000004122 cyclic group Chemical group 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000000499 gel Substances 0.000 description 3
- 230000005847 immunogenicity Effects 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 229930027917 kanamycin Natural products 0.000 description 3
- 229960000318 kanamycin Drugs 0.000 description 3
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 3
- 229930182823 kanamycin A Natural products 0.000 description 3
- 150000002632 lipids Chemical class 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 230000001404 mediated effect Effects 0.000 description 3
- 238000010369 molecular cloning Methods 0.000 description 3
- 238000007857 nested PCR Methods 0.000 description 3
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 3
- 238000005215 recombination Methods 0.000 description 3
- 230000006798 recombination Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000013518 transcription Methods 0.000 description 3
- 230000035897 transcription Effects 0.000 description 3
- 238000001890 transfection Methods 0.000 description 3
- 229940098232 yersinia enterocolitica Drugs 0.000 description 3
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- 241000710929 Alphavirus Species 0.000 description 2
- 101100437895 Alternaria brassicicola bsc3 gene Proteins 0.000 description 2
- 101100165663 Alternaria brassicicola bsc8 gene Proteins 0.000 description 2
- 229920000856 Amylose Polymers 0.000 description 2
- 102100020724 Ankyrin repeat, SAM and basic leucine zipper domain-containing protein 1 Human genes 0.000 description 2
- 101100173302 Bacillus subtilis (strain 168) fatR gene Proteins 0.000 description 2
- 241001647378 Chlamydia psittaci Species 0.000 description 2
- 102100031725 Cortactin-binding protein 2 Human genes 0.000 description 2
- 241000588694 Erwinia amylovora Species 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- 241000606768 Haemophilus influenzae Species 0.000 description 2
- 101000785414 Homo sapiens Ankyrin repeat, SAM and basic leucine zipper domain-containing protein 1 Proteins 0.000 description 2
- 101000941045 Homo sapiens Cortactin-binding protein 2 Proteins 0.000 description 2
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 2
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 2
- 101000819644 Lysinibacillus sphaericus UPF0309 protein in nagA 3'region Proteins 0.000 description 2
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 2
- 241000699666 Mus <mouse, genus> Species 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- 101001055788 Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) Pentapeptide repeat protein MfpA Proteins 0.000 description 2
- 241000588653 Neisseria Species 0.000 description 2
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 2
- 101150073872 ORF3 gene Proteins 0.000 description 2
- 101100226896 Phomopsis amygdali PaMT gene Proteins 0.000 description 2
- 101100226891 Phomopsis amygdali PaP450-1 gene Proteins 0.000 description 2
- 241000709664 Picornaviridae Species 0.000 description 2
- 108010052646 Protein Translocation Systems Proteins 0.000 description 2
- 102000018819 Protein Translocation Systems Human genes 0.000 description 2
- 101100218584 Pseudomonas fluorescens (strain SBW25) bscS gene Proteins 0.000 description 2
- 241000589615 Pseudomonas syringae Species 0.000 description 2
- 241000607142 Salmonella Species 0.000 description 2
- 241000607768 Shigella Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 101000901034 Streptococcus gordonii Accessory Sec system protein Asp2 Proteins 0.000 description 2
- 101000936719 Streptococcus gordonii Accessory Sec system protein Asp3 Proteins 0.000 description 2
- 101000936711 Streptococcus gordonii Accessory secretory protein Asp4 Proteins 0.000 description 2
- 101000936720 Streptococcus gordonii Accessory secretory protein Asp5 Proteins 0.000 description 2
- 101000912941 Streptococcus gordonii DegV domain-containing protein Proteins 0.000 description 2
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 2
- 108020004566 Transfer RNA Proteins 0.000 description 2
- 108010020764 Transposases Proteins 0.000 description 2
- 241000589884 Treponema pallidum Species 0.000 description 2
- 206010046865 Vaccinia virus infection Diseases 0.000 description 2
- 241000607626 Vibrio cholerae Species 0.000 description 2
- 241000589636 Xanthomonas campestris Species 0.000 description 2
- 241000815873 Xanthomonas euvesicatoria Species 0.000 description 2
- 108700041761 Yersinia LcrD Proteins 0.000 description 2
- 239000002671 adjuvant Substances 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 125000000539 amino acid group Chemical group 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 239000013611 chromosomal DNA Substances 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 238000004590 computer program Methods 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 238000004520 electroporation Methods 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 238000007429 general method Methods 0.000 description 2
- 101150004480 greA gene Proteins 0.000 description 2
- 229940047650 haemophilus influenzae Drugs 0.000 description 2
- 239000003228 hemolysin Substances 0.000 description 2
- FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 230000002458 infectious effect Effects 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 2
- 210000004072 lung Anatomy 0.000 description 2
- 229930182817 methionine Natural products 0.000 description 2
- 230000009456 molecular mechanism Effects 0.000 description 2
- 238000010172 mouse model Methods 0.000 description 2
- 229940037201 oris Drugs 0.000 description 2
- WEXRUCMBJFQVBZ-UHFFFAOYSA-N pentobarbital Chemical compound CCCC(C)C1(CC)C(=O)NC(=O)NC1=O WEXRUCMBJFQVBZ-UHFFFAOYSA-N 0.000 description 2
- 108010021711 pertactin Proteins 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000004393 prognosis Methods 0.000 description 2
- 238000003259 recombinant expression Methods 0.000 description 2
- 238000010188 recombinant method Methods 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000010361 transduction Methods 0.000 description 2
- 230000026683 transduction Effects 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- 230000034512 ubiquitination Effects 0.000 description 2
- 238000010798 ubiquitination Methods 0.000 description 2
- 241000701161 unidentified adenovirus Species 0.000 description 2
- 241001515965 unidentified phage Species 0.000 description 2
- 229940125575 vaccine candidate Drugs 0.000 description 2
- 208000007089 vaccinia Diseases 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 101150096623 yscU gene Proteins 0.000 description 2
- TVZGACDUOSZQKY-LBPRGKRZSA-N 4-aminofolic acid Chemical compound C1=NC2=NC(N)=NC(N)=C2N=C1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 TVZGACDUOSZQKY-LBPRGKRZSA-N 0.000 description 1
- ODHCTXKNWHHXJC-VKHMYHEASA-N 5-oxo-L-proline Chemical compound OC(=O)[C@@H]1CCC(=O)N1 ODHCTXKNWHHXJC-VKHMYHEASA-N 0.000 description 1
- 230000005730 ADP ribosylation Effects 0.000 description 1
- 102000007469 Actins Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 1
- 241001550224 Apha Species 0.000 description 1
- 206010003445 Ascites Diseases 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 241000972773 Aulopiformes Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 108700005014 Bacteria FlhA Proteins 0.000 description 1
- 108020000946 Bacterial DNA Proteins 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 241000221198 Basidiomycota Species 0.000 description 1
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 1
- 241000588780 Bordetella parapertussis Species 0.000 description 1
- 241000589562 Brucella Species 0.000 description 1
- 241000589876 Campylobacter Species 0.000 description 1
- 241000222122 Candida albicans Species 0.000 description 1
- 108091033380 Coding strand Proteins 0.000 description 1
- 108700010070 Codon Usage Proteins 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 102000016917 Complement C1 Human genes 0.000 description 1
- 108010028774 Complement C1 Proteins 0.000 description 1
- 108010028776 Complement C7 Proteins 0.000 description 1
- 108010027644 Complement C9 Proteins 0.000 description 1
- 241000218631 Coniferophyta Species 0.000 description 1
- 238000011537 Coomassie blue staining Methods 0.000 description 1
- 241000192700 Cyanobacteria Species 0.000 description 1
- LEVWYRKDKASIDU-QWWZWVQMSA-N D-cystine Chemical compound OC(=O)[C@H](N)CSSC[C@@H](N)C(O)=O LEVWYRKDKASIDU-QWWZWVQMSA-N 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 230000006820 DNA synthesis Effects 0.000 description 1
- 102000016911 Deoxyribonucleases Human genes 0.000 description 1
- 108010053770 Deoxyribonucleases Proteins 0.000 description 1
- 241000702421 Dependoparvovirus Species 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 241000588921 Enterobacteriaceae Species 0.000 description 1
- 102100029727 Enteropeptidase Human genes 0.000 description 1
- 108010013369 Enteropeptidase Proteins 0.000 description 1
- 241000709661 Enterovirus Species 0.000 description 1
- 241000991587 Enterovirus C Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 208000000832 Equine Encephalomyelitis Diseases 0.000 description 1
- 101100451480 Erwinia amylovora hrpI gene Proteins 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 101100111747 Eupenicillium brefeldianum Bref-PKS gene Proteins 0.000 description 1
- 208000000666 Fowlpox Diseases 0.000 description 1
- 241000700662 Fowlpox virus Species 0.000 description 1
- 241000589602 Francisella tularensis Species 0.000 description 1
- 102000002464 Galactosidases Human genes 0.000 description 1
- 108010093031 Galactosidases Proteins 0.000 description 1
- 229930182566 Gentamicin Natural products 0.000 description 1
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 1
- 102000005720 Glutathione transferase Human genes 0.000 description 1
- 108010070675 Glutathione transferase Proteins 0.000 description 1
- HVLSXIKZNLPZJJ-TXZCQADKSA-N HA peptide Chemical group C([C@@H](C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](C)C(O)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](N)CC=1C=CC(O)=CC=1)C1=CC=C(O)C=C1 HVLSXIKZNLPZJJ-TXZCQADKSA-N 0.000 description 1
- 208000032843 Hemorrhage Diseases 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 108010093488 His-His-His-His-His-His Proteins 0.000 description 1
- 241000701085 Human alphaherpesvirus 3 Species 0.000 description 1
- UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 1
- 229930010555 Inosine Natural products 0.000 description 1
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- 108090001090 Lectins Proteins 0.000 description 1
- 102000004856 Lectins Human genes 0.000 description 1
- 241000589242 Legionella pneumophila Species 0.000 description 1
- 241000186781 Listeria Species 0.000 description 1
- 241000218922 Magnoliophyta Species 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 241000588621 Moraxella Species 0.000 description 1
- 241000588655 Moraxella catarrhalis Species 0.000 description 1
- 241000699660 Mus musculus Species 0.000 description 1
- 241000588650 Neisseria meningitidis Species 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
- 108010038807 Oligopeptides Proteins 0.000 description 1
- 102000015636 Oligopeptides Human genes 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 241000588912 Pantoea agglomerans Species 0.000 description 1
- 241000606860 Pasteurella Species 0.000 description 1
- 108010081690 Pertussis Toxin Proteins 0.000 description 1
- 101100226888 Phomopsis amygdali PaAT-2 gene Proteins 0.000 description 1
- 101100226895 Phomopsis amygdali PaP450-3 gene Proteins 0.000 description 1
- 101100226887 Phomopsis amygdali PaP450-5 gene Proteins 0.000 description 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
- 206010035226 Plasma cell myeloma Diseases 0.000 description 1
- 241000276498 Pollachius virens Species 0.000 description 1
- 241000588770 Proteus mirabilis Species 0.000 description 1
- 101100502918 Proteus mirabilis flhA gene Proteins 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241000218936 Pseudomonas corrugata Species 0.000 description 1
- 108700001939 Pseudomonas syringae hrpI Proteins 0.000 description 1
- 101100451485 Pseudomonas syringae pv. syringae hrpL gene Proteins 0.000 description 1
- 238000003559 RNA-seq method Methods 0.000 description 1
- 241000589771 Ralstonia solanacearum Species 0.000 description 1
- 108700015767 Ralstonia solanacearum HrpO Proteins 0.000 description 1
- 108700008625 Reporter Genes Proteins 0.000 description 1
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 1
- 241000235070 Saccharomyces Species 0.000 description 1
- 108700006983 Salmonella typhimurium SpaS Proteins 0.000 description 1
- 241000710961 Semliki Forest virus Species 0.000 description 1
- 241000607762 Shigella flexneri Species 0.000 description 1
- 108700025705 Shigella flexneri mxiA Proteins 0.000 description 1
- 101100132345 Shigella flexneri mxiA gene Proteins 0.000 description 1
- 101100149987 Shigella flexneri spaS gene Proteins 0.000 description 1
- 241000710960 Sindbis virus Species 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 241000256248 Spodoptera Species 0.000 description 1
- 241000295644 Staphylococcaceae Species 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- 241000187747 Streptomyces Species 0.000 description 1
- 241000701093 Suid alphaherpesvirus 1 Species 0.000 description 1
- 241000192581 Synechocystis sp. Species 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- 101710117260 Uracil permease Proteins 0.000 description 1
- 101100502921 Yersinia enterocolitica flhA gene Proteins 0.000 description 1
- 101100502934 Yersinia enterocolitica flhB gene Proteins 0.000 description 1
- 101100127725 Yersinia enterocolitica lcrD gene Proteins 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 101100398739 Yersinia pestis lcrH gene Proteins 0.000 description 1
- 241000607477 Yersinia pseudotuberculosis Species 0.000 description 1
- 101100267948 Yersinia pseudotuberculosis serotype I (strain IP32953) yscO gene Proteins 0.000 description 1
- 230000021736 acetylation Effects 0.000 description 1
- 238000006640 acetylation reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000010933 acylation Effects 0.000 description 1
- 238000005917 acylation reaction Methods 0.000 description 1
- 102000030621 adenylate cyclase Human genes 0.000 description 1
- 108060000200 adenylate cyclase Proteins 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009435 amidation Effects 0.000 description 1
- 238000007112 amidation reaction Methods 0.000 description 1
- 229960003896 aminopterin Drugs 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 238000012870 ammonium sulfate precipitation Methods 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 238000005571 anion exchange chromatography Methods 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- 230000005875 antibody response Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000010516 arginylation Effects 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 230000036760 body temperature Effects 0.000 description 1
- 238000006664 bond formation reaction Methods 0.000 description 1
- 101150083238 bsc7 gene Proteins 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 229940095731 candida albicans Drugs 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 238000005277 cation exchange chromatography Methods 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000022131 cell cycle Effects 0.000 description 1
- 230000003915 cell function Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000007385 chemical modification Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 108091008690 chemoreceptors Proteins 0.000 description 1
- 239000003593 chromogenic compound Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 229960003067 cystine Drugs 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 230000017858 demethylation Effects 0.000 description 1
- 238000010520 demethylation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 229960002086 dextran Drugs 0.000 description 1
- 229960000633 dextran sulfate Drugs 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 231100000673 dose–response relationship Toxicity 0.000 description 1
- 210000002472 endoplasmic reticulum Anatomy 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 210000001842 enterocyte Anatomy 0.000 description 1
- 230000026502 entry into host cell Effects 0.000 description 1
- 238000012869 ethanol precipitation Methods 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 101150008469 flhA gene Proteins 0.000 description 1
- 230000022244 formylation Effects 0.000 description 1
- 238000006170 formylation reaction Methods 0.000 description 1
- 229940118764 francisella tularensis Drugs 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 230000006251 gamma-carboxylation Effects 0.000 description 1
- 108091008053 gene clusters Proteins 0.000 description 1
- 230000013595 glycosylation Effects 0.000 description 1
- 238000006206 glycosylation reaction Methods 0.000 description 1
- 244000000058 gram-negative pathogen Species 0.000 description 1
- 150000003278 haem Chemical group 0.000 description 1
- 125000000487 histidyl group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C([H])=N1 0.000 description 1
- 239000012510 hollow fiber Substances 0.000 description 1
- 244000052637 human pathogen Species 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 238000004191 hydrophobic interaction chromatography Methods 0.000 description 1
- 238000012872 hydroxylapatite chromatography Methods 0.000 description 1
- 230000033444 hydroxylation Effects 0.000 description 1
- 238000005805 hydroxylation reaction Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000003119 immunoblot Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000006882 induction of apoptosis Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 229960003786 inosine Drugs 0.000 description 1
- 239000002198 insoluble material Substances 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000007918 intramuscular administration Methods 0.000 description 1
- 238000001990 intravenous administration Methods 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 230000026045 iodination Effects 0.000 description 1
- 238000006192 iodination reaction Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 101150064142 lcrH gene Proteins 0.000 description 1
- 239000002523 lectin Substances 0.000 description 1
- 229940115932 legionella pneumophila Drugs 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 210000001165 lymph node Anatomy 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 201000001441 melanoma Diseases 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
- 230000011987 methylation Effects 0.000 description 1
- 238000007069 methylation reaction Methods 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 201000000050 myeloid neoplasm Diseases 0.000 description 1
- 230000007498 myristoylation Effects 0.000 description 1
- 210000004897 n-terminal region Anatomy 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 239000002751 oligonucleotide probe Substances 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000007911 parenteral administration Methods 0.000 description 1
- 230000008506 pathogenesis Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 229960001412 pentobarbital Drugs 0.000 description 1
- 210000001322 periplasm Anatomy 0.000 description 1
- 229940066827 pertussis vaccine Drugs 0.000 description 1
- 238000002823 phage display Methods 0.000 description 1
- 239000008194 pharmaceutical composition Substances 0.000 description 1
- 229940080469 phosphocellulose Drugs 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 230000003032 phytopathogenic effect Effects 0.000 description 1
- 244000000003 plant pathogen Species 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 230000004481 post-translational protein modification Effects 0.000 description 1
- 230000013823 prenylation Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000002987 primer (paints) Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000004952 protein activity Effects 0.000 description 1
- 230000009145 protein modification Effects 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 229940023143 protein vaccine Drugs 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 238000003906 pulsed field gel electrophoresis Methods 0.000 description 1
- 229940043131 pyroglutamate Drugs 0.000 description 1
- 230000006340 racemization Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000004153 renaturation Methods 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 238000007363 ring formation reaction Methods 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 101150008186 sctN gene Proteins 0.000 description 1
- 230000009962 secretion pathway Effects 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- LYZQCTRTDQFROA-UHFFFAOYSA-N sodium 4-hydroxy-7-[[5-hydroxy-7-sulfo-6-[(6-sulfonaphthalen-2-yl)diazenyl]naphthalen-2-yl]carbamoylamino]-3-phenyldiazenylnaphthalene-2-sulfonic acid Chemical compound C1=CC=C(C=C1)N=NC2=C(C=C3C=C(C=CC3=C2O)NC(=O)NC4=CC5=CC(=C(C(=C5C=C4)O)N=NC6=CC7=C(C=C6)C=C(C=C7)S(=O)(=O)O)S(=O)(=O)O)S(=O)(=O)O.[Na+] LYZQCTRTDQFROA-UHFFFAOYSA-N 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 101150065015 spa gene Proteins 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 230000019635 sulfation Effects 0.000 description 1
- 238000005670 sulfation reaction Methods 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 238000001308 synthesis method Methods 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 229940104230 thymidine Drugs 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 238000011830 transgenic mouse model Methods 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 229940038773 trisodium citrate Drugs 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 238000005199 ultracentrifugation Methods 0.000 description 1
- 230000009452 underexpressoin Effects 0.000 description 1
- 241000701447 unidentified baculovirus Species 0.000 description 1
- 241001529453 unidentified herpesvirus Species 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 229940118696 vibrio cholerae Drugs 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 101150117012 yopN gene Proteins 0.000 description 1
- 101150061948 yscI gene Proteins 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/235—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bordetella (G)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Physics & Mathematics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Gastroenterology & Hepatology (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB9828217 | 1998-12-21 | ||
GBGB9828217.1A GB9828217D0 (en) | 1998-12-21 | 1998-12-21 | Vacine |
PCT/EP1999/010297 WO2000037493A2 (fr) | 1998-12-21 | 1999-12-21 | Vaccin |
Publications (1)
Publication Number | Publication Date |
---|---|
AU2903700A true AU2903700A (en) | 2000-07-12 |
Family
ID=10844715
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU29037/00A Abandoned AU2903700A (en) | 1998-12-21 | 1999-12-21 | Vaccine |
Country Status (8)
Country | Link |
---|---|
EP (1) | EP1140996A2 (fr) |
JP (1) | JP2002534960A (fr) |
CN (1) | CN1371389A (fr) |
AU (1) | AU2903700A (fr) |
CA (1) | CA2356764A1 (fr) |
GB (1) | GB9828217D0 (fr) |
HK (1) | HK1041490A1 (fr) |
WO (1) | WO2000037493A2 (fr) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10465252B2 (en) * | 2007-10-26 | 2019-11-05 | Quest Diagnostics Investments Incorporated | Bordetella detection assay |
WO2009059379A1 (fr) * | 2007-11-07 | 2009-05-14 | Dynamic Microbials Limited | Compositions et formulations antimicrobiennes et leurs utilisations |
CN101875974A (zh) * | 2010-07-02 | 2010-11-03 | 深圳市儿童医院 | 检测百日咳杆菌的引物组、检测试剂盒以及检测方法 |
AU2019360106A1 (en) * | 2018-10-15 | 2021-05-13 | Arizona Board Of Regents On Behalf Of The University Of Arizona | Vaccine polypeptide compositions and methods |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1999059630A1 (fr) * | 1998-05-15 | 1999-11-25 | University Of California Los Angeles | Systeme de secretion de bordetella de type iii |
-
1998
- 1998-12-21 GB GBGB9828217.1A patent/GB9828217D0/en not_active Ceased
-
1999
- 1999-12-21 JP JP2000589562A patent/JP2002534960A/ja active Pending
- 1999-12-21 CN CN99816279A patent/CN1371389A/zh active Pending
- 1999-12-21 AU AU29037/00A patent/AU2903700A/en not_active Abandoned
- 1999-12-21 CA CA002356764A patent/CA2356764A1/fr not_active Abandoned
- 1999-12-21 EP EP99969578A patent/EP1140996A2/fr not_active Withdrawn
- 1999-12-21 WO PCT/EP1999/010297 patent/WO2000037493A2/fr not_active Application Discontinuation
-
2002
- 2002-02-19 HK HK02101214.6A patent/HK1041490A1/zh unknown
Also Published As
Publication number | Publication date |
---|---|
EP1140996A2 (fr) | 2001-10-10 |
WO2000037493A3 (fr) | 2001-02-01 |
WO2000037493A2 (fr) | 2000-06-29 |
JP2002534960A (ja) | 2002-10-22 |
HK1041490A1 (zh) | 2002-07-12 |
GB9828217D0 (en) | 1999-02-17 |
CN1371389A (zh) | 2002-09-25 |
CA2356764A1 (fr) | 2000-06-29 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Charles et al. | Molecular cloning and characterization of protective outer membrane protein P. 69 from Bordetella pertussis. | |
CA2347849C (fr) | Proteines omp85 de neisseria gonorrhoeae et de neisseria meningitidis, compositions renfermant lesdites proteines et methodes d'utilisation correspondantes | |
US5476657A (en) | Pasteurella haemolytica leukotoxin compositions and uses thereof | |
KR20060134227A (ko) | 신규한 스트렙토코커스 항원 | |
JP2006166919A (ja) | 空胞形成毒素欠損H.pyloriおよび関連する方法 | |
AU642650B2 (en) | Compositions and treatments for pneumonia in animals | |
Pedroni et al. | Cloning of a novel pilin‐like gene from Bordetella pertussis: homology to the fim2 gene | |
AU718392B2 (en) | Haemophilus adhesion proteins | |
EP0719155A1 (fr) | ANTIGENES DE $i(CAMPYLOBACTER JEJUNI), LEURS PROCEDES DE PRODUCTION ET LEUR UTILISATION | |
US6669940B2 (en) | Recombinant fusobacterium necrophorum leukotoxin vaccine and preparation thereof | |
US5849531A (en) | Compositions and treatments for pneumonia in animals | |
US6610306B2 (en) | OMP85 protein of neisseria meningitidis, compositions containing the same and methods of use thereof | |
US20040096973A1 (en) | Environmentally regulated genes of Streptococcus suis | |
US6617128B2 (en) | Nucleic acid molecules encoding proteins which impart the adhesion of neisseria cells to human cells | |
US20040219585A1 (en) | Nontypeable haemophilus influenzae virulence factors | |
AU2903700A (en) | Vaccine | |
EP1060249A1 (fr) | PEPTIDES EPITOPES IMMUNOGENES CONTRE L'INFECTION PAR $i(STREPTOCOCCUS PNEUMONIAE) | |
US20040047871A1 (en) | Recombinant fusobacterium necrophorum leukotoxin vaccine and prepaation thereof | |
EP1535928B1 (fr) | Vaccin comprenant Omp85 protéines de Neisseria gonorhoeae et Neisseria meningitidis | |
CA2383557A1 (fr) | Antigenes de vaccin provenant de moraxella | |
WO2000071724A2 (fr) | Composes nouveaux | |
WO1992017587A1 (fr) | ANTIGENE DE MEMBRANE EXTERIEURE DE $i(BORDETELLA BRONCHISEPTICA) |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
MK5 | Application lapsed section 142(2)(e) - patent request and compl. specification not accepted |