AU2536899A - Substituted 1,2,4-triazolo(3,4-a)pyridazine - Google Patents
Substituted 1,2,4-triazolo(3,4-a)pyridazine Download PDFInfo
- Publication number
- AU2536899A AU2536899A AU25368/99A AU2536899A AU2536899A AU 2536899 A AU2536899 A AU 2536899A AU 25368/99 A AU25368/99 A AU 25368/99A AU 2536899 A AU2536899 A AU 2536899A AU 2536899 A AU2536899 A AU 2536899A
- Authority
- AU
- Australia
- Prior art keywords
- triazolo
- compound
- methylisoxazol
- methyloxy
- triazol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
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- XSCHRSMBECNVNS-UHFFFAOYSA-N benzopyrazine Natural products N1=CC=NC2=CC=CC=C21 XSCHRSMBECNVNS-UHFFFAOYSA-N 0.000 claims description 2
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- LFSXCDWNBUNEEM-UHFFFAOYSA-N phthalazine Chemical compound C1=NN=CC2=CC=CC=C21 LFSXCDWNBUNEEM-UHFFFAOYSA-N 0.000 claims description 2
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- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 239000012053 oil suspension Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 239000003182 parenteral nutrition solution Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000000312 peanut oil Substances 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 239000008024 pharmaceutical diluent Substances 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000000216 proconvulsive effect Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- SHNUBALDGXWUJI-UHFFFAOYSA-N pyridin-2-ylmethanol Chemical compound OCC1=CC=CC=N1 SHNUBALDGXWUJI-UHFFFAOYSA-N 0.000 description 1
- 238000000611 regression analysis Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000008159 sesame oil Substances 0.000 description 1
- 235000011803 sesame oil Nutrition 0.000 description 1
- 239000004208 shellac Substances 0.000 description 1
- 229940113147 shellac Drugs 0.000 description 1
- ZLGIYFNHBLSMPS-ATJNOEHPSA-N shellac Chemical compound OCCCCCC(O)C(O)CCCCCCCC(O)=O.C1C23[C@H](C(O)=O)CCC2[C@](C)(CO)[C@@H]1C(C(O)=O)=C[C@@H]3O ZLGIYFNHBLSMPS-ATJNOEHPSA-N 0.000 description 1
- 235000013874 shellac Nutrition 0.000 description 1
- 239000012279 sodium borohydride Substances 0.000 description 1
- 229910000033 sodium borohydride Inorganic materials 0.000 description 1
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 1
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 1
- 239000008247 solid mixture Substances 0.000 description 1
- 239000001593 sorbitan monooleate Substances 0.000 description 1
- 235000011069 sorbitan monooleate Nutrition 0.000 description 1
- 229940035049 sorbitan monooleate Drugs 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 235000010356 sorbitol Nutrition 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000008117 stearic acid Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000829 suppository Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000000454 talc Substances 0.000 description 1
- 229910052623 talc Inorganic materials 0.000 description 1
- 235000012222 talc Nutrition 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 239000000196 tragacanth Substances 0.000 description 1
- 235000010487 tragacanth Nutrition 0.000 description 1
- 229940116362 tragacanth Drugs 0.000 description 1
- 208000018726 traumatic encephalopathy Diseases 0.000 description 1
- ILWRPSCZWQJDMK-UHFFFAOYSA-N triethylazanium;chloride Chemical compound Cl.CCN(CC)CC ILWRPSCZWQJDMK-UHFFFAOYSA-N 0.000 description 1
- RZLMQJSSMROKSS-UHFFFAOYSA-N trimethyl-[2-(triazol-1-ylmethoxy)ethyl]silane Chemical compound C[Si](C)(C)CCOCN1C=CN=N1 RZLMQJSSMROKSS-UHFFFAOYSA-N 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
- C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
- C07D487/04—Ortho-condensed systems
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
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- Hospice & Palliative Care (AREA)
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Description
WO 99/43677 PCT/GB99/00485 SUBSTITUTED 1,2,4-TRIAZOLO 3,4-al PYRIDAZINE The present invention relates to a substituted triazolo-pyridazine derivative, to its use in therapy, to compositions containing it and to a process for 5 its manufacture. We have now discovered that it is possible to obtain medicaments which have cognition enhancing effects which may be employed with less risk of proconvulsant effects previously described with benzodiazepine receptor partial or full inverse agonists. Inverse agonists which are inverse agonists for the a5 10 receptor and are relatively free of activity at al, u2 and a3 receptor binding sites are preferred. European Patent Applications 0085840 and 0134946 describe related series of 1,2,4-triazolo[3,4-a]phthalazine derivatives which are stated to possess antianxiety activity. However, there is no disclosure nor any suggestion in either 15 of these publications of the compounds of the present invention, nor that the compounds disclosed in the Applications have any cognition enhancing properties. The present invention provides a compound which is: 3-(5-methylisoxazol-3-yl)-6-(1-methyl-1,2,3-triazol-4-yl)methyloxy- 1,2,4 20 triazolo[3,4-a]phthalazine. Cognition enhancement can be shown by testing the compound in the Morris watermaze as reported by McNamara and Skelton, Psychobiology, 21:101 108. The functional efficacy at the various receptor subtypes can be calculated using the method disclosed in WO-A-9625948. 25 Thus, for example, the compound of the present invention can be used in a variety of disorders of the central nervous system. Such disorders include delirium, dementia and amnestic and other cognitive disorders. Examples of delirium are delirium due to substance intoxication or substance withdrawal, delirium due to multiple etiologies and delirium NOS (not otherwise specified). 30 Examples of dementia are: dementia of the Alzheimer's type with early onset which can be uncomplicated or with delirium, delusions or depressed mood; dementia of the Alzheimer's type, with late onset, which can be uncomplicated or with delirium, delusions or depressed mood; vascular dementia which can be uncomplicated or with delirium, delusions or depressed mood; dementia due to WO 99/43677 - 2 - PCT/GB99/00485 HIV disease; dementia due to head trauma; dementia due to Parkinson's disease; dementia due to Huntington's disease; dementia due to Pick's disease; dementia due to Creutzfeld-Jakob disease; dementia which is substance-induced persisting or due to multiple etiologies; and dementia NOS. Examples of amnestic 5 disorders are amnestic disorder due to a particular medical condition or which is substance-induced persisting or which is amnestic disorder NOS. The invention also provides pharmaceutical compositions comprising the compound of this invention and a pharmaceutically acceptable carrier. Preferably these compositions are in unit dosage forms such as tablets, pills, 10 capsules, powders, granules, sterile parenteral solutions or suspensions, metered aerosol or liquid sprays, drops, ampoules, transdermal patches, auto-injector devices or suppositories; for oral, parenteral, intranasal, sublingual or rectal administration, or for administration by inhalation or insufflation. For preparing solid compositions such as tablets, the principal active ingredient is 15 mixed with a pharmaceutical carrier, e.g. conventional tableting ingredients such as corn starch, lactose, sucrose, sorbitol, talc, stearic acid, magnesium stearate, dicalcium phosphate or gums or surfactants such as sorbitan monooleate, polyethylene glycel, and other pharmaceutical diluents, e.g. water, to form a solid preformulation composition containing a homogeneous mixture of the compound 20 of the present invention, or a pharmaceutically acceptable salt thereof. When referring to these preformulation compositions as homogeneous, it is meant that the active ingredient is dispersed evenly throughout the composition so that the composition may be readily subdivided into equally effective unit dosage forms such as tablets, pills and capsules. This solid preformulation composition is then 25 subdivided into unit dosage forms of the type described above containing from 0.1 to about 500 mg of the active ingredient of the present invention. Typical unit dosage forms contain from 1 to 100 mg, for example 1, 2, 5, 10, 25, 50 or 100 mg, of the active ingredient. The tablets or pills of the novel composition can be coated or otherwise compounded to provide a dosage form affording the 30 advantage of prolonged action. For example, the tablet or pill can comprise an inner dosage and an outer dosage component, the latter being in the form of an envelope over the former. The two components can be separated by an enteric layer which serves to resist disintegration in the stomach and permits the inner component to pass intact into the duodenum or to be delayed in release. A WO 99/43677 - 3 - PCT/GB99/00485 variety of materials can be used for such enteric layers or coatings, such materials including a number of polymeric acids and mixtures of polymneric acids with such materials as shellac, cetyl alcohol and cellulose acetate. The liquid forms in which the novel compositions of the present invention 5 may be incorporated for administration orally or by injection include aqueous solutions, suitably flavoured syrups, aqueous or oil suspensions, and flavoured emulsions with edible oils such as cottonseed oil, sesame oil, coconut oil or peanut oil, as well as elixirs and similar pharmaceutical vehicles. Suitable dispersing or suspending agents for aqueous suspensions include synthetic and 10 natural gums such as tragacanth, acacia, alginate, dextran, sodium carboxymethylcellulose, methylcellulose, polyvinyl-pyrrolidone or gelatin. The present invention further provides the use of the compound of the present invention in the manufacture of a medicament for the enhancement of cognition, preferably in a human suffering from a dementing illness such as 15 Alzheimer's disease. For the enhancement of cognition, a suitable dosage level is about 0.01 to 250 mg/kg per day, preferably about 0.01 to 100 mg/kg per day, and especially about 0.01 to 5 mg/kg of body weight per day. The compound may be administered on a regimen of 1 to 4 times per day. In some cases, however, 20 dosage outside these limits may be used. It is preferred that the compound of the present invention be ground, for example using a pestle and mortar or industrial equivalent thereto, to a particle size of between 1 and 10 pM, and preferably less than 5 pM, before formulation. The compound may be micronised or sonicised by methods known in the art or 25 nanonised, for example by methods disclosed in US-A-5145684. The present invention also provides a process for the production of the compound of the present invention, which comprises reacting a methylating reagent, such as lithium hexamethyldisilazide with 3-(5-methylisoxazol-3-yl)-6 (1H-1,2,3-triazol-5-yl)methyloxy-1,2,4-triazolo[3,4-a]phthalazine. 30 The reaction is generally carried out in a solvent such as DMF, generally at a temperature below 0 0 C with warming to about room temperature and generally under an inert gas such as nitrogen. The reaction mixture is generally allowed to stand for 4-12 hours. The desired product is generally purified from WO 99/43677 - 4 - PCT/GB99/00485 other reaction products by a conventional separation technique such as preparative HPLC. During any of the above synthetic sequences it may be necessary and/or desirable to protect sensitive or reactive groups on any of the molecules 5 concerned. This may be achieved by means of conventional protecting groups, such as those described in Protective Groups in Organic Chemistry, ed. J.F.W. McOmie, Plenum Press, 1973; and T.W. Greene & P.G.M. Wuts, Protective Groups in Organic Synthesis, John Wiley & Sons, 1991. The protecting groups may be removed at a convenient subsequent stage using methods known from 10 the art. The compound in accordance with this invention potently inhibit the binding of [ 3 H]-flumazenil to the benzodiazepine binding site of human GABAA receptors containing the a5 subunit stably expressed in Ltk cells. Reagents 15 * Phosphate buffered saline (PBS). * Assay buffer: 10 mM KH 2
PO
4 , 100 mM KC1, pH 7.4 at room temperature. * [ 3 H]-Flumazenil (18 nM for alp3y2 cells; 18 nM for a2p3y2 cells; 10 nM for a33y2 cells; 10 nM for a533y2 cells) in assay buffer. * Flunitrazepam 100 pM in assay buffer. 20 * Cells resuspended in assay buffer (1 tray to 10 ml). Harvesting Cells Supernatant is removed from cells. PBS (approximately 20 ml) is added. The cells are scraped and placed in a 50 ml centrifuge tube. The procedure is repeated with a further 10 ml of PBS to ensure that most of the cells are 25 removed. The cells are pelleted by centrifuging for 20 min at 3000 rpm in a benchtop centrifuge, and then frozen if desired. The pellets are resuspended in 10 ml of buffer per tray (25 cm x 25 cm) of cells. Assay Can be carried out in deep 96-well plates or in tubes. Each tube contains: 30 * 300 d of assay buffer. * 50 pl of [ 3 H]-flumazenil (final concentration for alp3y2: 1.8 nM; for a233y2: 1.8 nM; for a3133y2: 1.0 nM; for a5P337y2: 1.0 nM).
WO 99/43677 - 5 - PCT/GB99/00485 * 50 p1 of buffer or solvent carrier (e.g. 10% DMSO) if compound is dissolved in 10% DMSO (total); test compound or flunitrazepam (to determine non-specific binding), 10 gM final concentration. * 100 p1 of cells. 5 Assays are incubated for 1 hour at 40 0 C, then filtered using either a Tomtec or Brandel cell harvester onto GF/B filters followed by 3 x 3 ml washes with ice cold assay buffer. Filters are dried and counted by liquid scintillation counting. Expected values for total binding are 3000-4000 dpm for total counts and less than 200 dpm for non-specific binding if using liquid scintillation 10 counting, or 1500-2000 dpm for total counts and less than 200 dpm for non specific binding if counting with meltilex solid scintillant. Binding parameters are determined by non-linear least squares regression analysis, from which the inhibition constant Ki can be calculated for the test compound. The compound in accordance with this invention potently inhibits the 15 binding of [ 3 H]-flumazenil to the benzodiazepine binding site of human GABAA receptors containing the a5 subunit stably expressed in Ltk cells. The compound of the accompanying Example was tested in the above assay, and was found to possess a Ki value for displacement of [ 3 H]Ro 15-1788 from the a5 subunit of the human GABAA receptor of 100 nM or less. 20 The compound of the present invention has been shown to enhance cognition in the rat water maze test (Morris, Learning and Motivation, 1981, 12, 239ff). Further details of methodology for demonstrating that the present compounds enhance cognition can be found in WO-A-9625948. This has been demonstrated at a minimum effective dose of 0.3mg/kg at which the compound 25 of the present invention has 40% receptor occupancy. It has also been demonstrated at a dose of 3mg/kg. The compound of the present invention can be made as described in following Examples. Precise details of the reaction conditions, and obvious modifications of the reaction procedure, are well within the capabilities of the 30 skilled person.
WO 99/43677 - 6 - PCT/GB99/00485 INTERMEDIATE 1 6-Chloro-3-(5-Methylisoxazol-3-vl)- 1,2,4-triazolof3,4-alphthalazine a) l-Chloro-4-hvdrazinophthalazine 1,4-Dichlorophthalazine (20.0g, 0.100 mol) was added to a boiling solution 5 of hydrazine monohydrate (37.3 ml, 0.765 mol) in ethanol (500 ml) and the mixture heated at reflux for 0.5 h. The mixture was cooled to room temperature and the solid collected by filtration and washed with ether. The material was taken with n-butanol and ammonia solution (sp. gr. 0.91) and heated until the solid dissolved. The organic layer was separated, evaporated in vacuo and the 10 residue azeotroped with xylene (x2) and dried in vacuo to give the title-hydrazine (11.5 g, 59%), 1H NMR (250 MHz, d-DMSO) 5 7.84 - 8.04 (3H, m, Ar-H), 8.20 (1H, m, Ar-H); MS (ES 4 ) m/e 194 [MH] +. b) 5-Methylisoxazole-3-carboxvlic acid A mixture of acetonylacetone (10g, 88 mmol) and nitric acid (sp. gr. 1.42) / 15 water (2:3) (50 ml) was cautiously brought to reflux under a stream of nitrogen and boiled for lh. The solution was cooled to room temperature and aged overnight. The resultant solid was collected by filtration, washed with chilled water (2 x 7 ml) and hexane, and dried in vacuo to give the title-acid (4.4g, 40%), 1H NMR (CDC13) 8 2.50 (3H, d, J=0.8Hz, Me), 6.41 (1H, d, J=0.8Hz, Ar-H). 20 c) 6 -Chloro-3-(5-Methvlisoxazol-3-v1)-1,2,4-triazolo[3,4-alphthalazine 5-Methylisoxazole-3-carboxylic acid (5.24 g, 41.3 mmol), bis(2-oxo-3 oxazolidinyl)phosphinic chloride (10.5 g, 41.2 mmol) and triethylamine (11.5 ml, 82.5 mmol) were added successively to a stirred suspension of 1-chloro-4 hydrazinophthalazine (8.00 g, 41.2 mmol) in dichloromethane (11) at 0 oC under 25 nitrogen. The mixture was stirred at 0 oC for 2h and at room temperature overnight. The solvent was evaporated in vacuo, the residue triturated with water and the solid filtered off, washed with hexane and dried in vacuo to give the ketohydrazine (11 g), MS (ES+) m/e 304 [MH]
+
. A solution of the ketohydrazine (11 g) and triethylamine hydrochloride (2.2 g, 20% w/w) in xylene 30 (500 ml) was heated at reflux for 3 h. The mixture was cooled to room temperature and the solvent evaporated in vacuo. The residue was dissolved in dichloromethane, washed with water (x 2), dried (MgSO4) and evaporated in vacuo, and the solid recrystallised (dichloromethane/hexane) to give the title- WO 99/43677 - 7 - PCT/GB99/00485 compound (6.8 g, 58%), 1H NMR (360MHz, CDC13) 8 2.59 (3H, s, Me), 6.90 (1H, s, Ar-H), 7.95 (1H, m, Ar-H), 8.07 (1H, m, Ar-H), 8.34 (1H, m, Ar-H), 8.78.(1H, s, Ar-H); MS (ES+) m/e 286 [MH] +. 5 REFERENCE EXAMPLE 1 3-(5-Methylisoxazol-3-v1)-6-(2-pyridv1)methvloxv- 1,2,4-triazolo[3,4-al phthalazine Sodium hydride (244 mg of a 60% dispersion in oil, 6.10 mmol) was added to a stirred solution of 2-pyridylcarbinol (470 mg, 4.27 mmol) in DMF (60 ml) at room temperature under nitrogen and the mixture stirred for 0.25 h. After this 10 time, Intermediate 1 (1160 mg, 4.07 mmol) was added and the mixture stirred for 2h. The solvent was removed in vacuo and the residue dissolved in dichloromethane, washed with water (x2), dried (MgSO4) and evaporated in vacuo. Flash chromatography on silica gel eluting with 3% methanol/dichloromethane followed by recrystallisation 15 (dichloromethane/hexane) gave the title-product (640 mg, 44%), mp 234 - 236 oC; 1 H NMR (360MHz, CDC13) 8 2.59 (3H, d, J =0.8Hz, Me), 5.77 (2H, s, CH2), 6.82 (1H, d, J=0.8Hz, Ar-H), 7.30 (1H, m, Ar-H), 7.74 - 7.85 (3H, m, Ar-H), 7.95 (1H, m, Ar-H), 8.33 (1H, d, J=7.8Hz, Ar-H), 8.64 - 8.72 (2H, m, Ar-H); MS (ES+) m/e 359 [MH]+; Anal. Found. C, 62.93; H, 3.56; N, 22.94. C19H 14
N
6 0 2 0.05 (CH 2 C1 2 ) 20 requires C, 63.10; H, 3.92; N, 23.17%. REFERENCE EXAMPLE 2 6-( 6 -Bromopyridin-2-v1)methyloxv-3-(5-methylisoxazol-3-vl)-1,2, 4-triazolo [3,4 a]lphthalazine 25 The title-compound was prepared from Intermediate 1 and 2 bromopyridine-6-methanol (Tetrahedron Lett., 1996, 50, 2537) following the procedure given for Reference Example 1. The product was isolated by addition of water to the reaction mixture and the resulting precipitate was filtered off. Flash chromatography on silica gel, eluting with ethyl acetate, and 30 recrystallisation (ethyl acetate-methanol) gave the title-phthalazine, mp 247.5 249 oC; 1H NMR (360 MHz, CDC1 3 ) 8 2.61 (3H, d, J = 0.7 Hz, Me), 5.73 (2H, s,
CH
2 ), 6.82 (1H, d, J = 0.7 Hz, Ar-H), 7.48 (1H, d, J = 7.8 Hz, Ar-H), 7.63 (1H, t, J = 7.7 Hz, Ar-H), 7.76 (1H, d, J = 7.4 Hz, Ar-H), 7.84 (1H, t, J = 8.4 Hz, Ar-H), WO 99/43677 - 8 - PCT/GB99/00485 7.98 (1H, t, J = 8.4 Hz, Ar-H), 8.31 (1H1, d, J = 8.5 Hz, Ar-H), 8.70 (1H, d, Ar-H); MS (ES
+
) m/e 437 [MH]+; Anal. Found C, 52.27; H, 2.85; N, 19.14. C19H13N602 Br. 0.1 (H20) requires C, 51.98; H, 3.03; N, 18.60%. 5 INTERMEDIATE 2 6-Hydroxy-3-(5-Methylisoxazol-3-vl)-1,2,4-triazolo [3,4-a]phthalazine A solution of sodium hydroxide (0.67 g, 17 mmol) in water (7.5 ml) was added to a stirred solution of Intermediate 1 (1.0 g, 3.5 mmol) in dioxane (37.5 ml) and the mixture heated at reflux for 4 h. The solvent was evaporated 10 in vacuo and the residue partitioned between water and diethyl ether. The aqueous layer was separated, washed with ether (x 1) and then acidified with 2N hydrochloric acid until pH2 was attained. The solid which precipitated out of solution was filtered off and the aqueous filtrate extracted with dichloromethane (x 3). The combined extracts were dried (MgSO4) and evaporated in vacuo and 15 combined with the precipitate to give the title-product (0.45 g, 48%), 1H NMR (250 MHz, d 6 -DMSO) 6 2.58 (3H, d, J = 0.7 Hz, Me), 7.07 (1H, d, J = 0.9 Hz, Ar-H), 7.94 (1H, m, Ar-H), 8.08 (1H, m, Ar-H), 8.24 (1H, d, J = 7.4 Hz, Ar-H), 8.54 (1H, d, J = 7.4 Hz, Ar-H), 13.32 (1H, br s, NH); MS (ES+) m/e 268 [MH]+ 20 REFERENCE EXAMPLE 3 3-(5-Methylisoxazol-3-v1)-6-(1H-1,2,3-triazol-5-v1)methyloxy-1,2,4 triazolo [3,4-alphthalazine a) 5-Formyl- 1- [2-(trimethvlsilvl)ethoxvlmethyl-1,2,3-triazole n-Butyl lithium (6.8ml of a 1.6M solution in hexanes, 10.9mmol) was 25 added dropwise over 0.08h to a stirred solution of 1-[2-(trimethyl silyl)ethoxy]methyl-1,2,3-triazole (J. Heterocycl. Chem., 1992, 29, 1203) (2.077g, 10.42mmol) in THF (30ml) at -78 0 C under nitrogen. The solution was allowed to warm to -60 0 C over 0.67h, then recooled to -78oC and DMF (0.9ml, 11.6mmol) added. The mixture was allowed to warm to room temperature and stirred for 30 16.5h. Saturated ammonium chloride solution (50ml) was added and the reaction mixture extracted with diethyl ether (3 x 80ml). The combined ethereal extrants were dried (MgSO4), evaporated in vacuo, and the residue chromatographed on silica gel, eluting with 30% ethyl acetate/hexane, to give the WO 99/43677 - 9 - PCT/GB99/00485 title-triazole (1.713g, 72%), 1H NMR (360 MH 2 , CDCl 3 ) 8 0.01 (9H, s, Me 3 Si), 0.92-0.99 (2H, m, CH 2 ), 3.64-3.69 (2H1, m, CH 2 ), 6.05 (2H, s, CH 2 ), 8.31 (1H, s, Ar-H), 10.12 (1H1, s, CHO). b) 5-Hydroxymethyl-1- [2-(trimethylsilyl)ethoxyvl methyl-1,2,3-triazole 5 Sodium borohydride (0.284g, 7.51mmol) was added to a stirred solution of the preceding triazole (1.704g, 7.495mmol) in methanol (8ml) at 0OC under nitrogen. The mixture was stirred at 0oC for 0.5h and at room temperature for 0.5h. Water was added and the mixture partitioned between dichloromethane and saturated brine. The aqueous layer was separated and further extracted 10 with dichloromethane (x2). The combined organic layers were dried ((MgSO4) and evaporated in vacuo and the residue chromatographed on silica gel, eluting with 70% ethyl acetate/hexane, to give the title-product (1.34g, 78%), 1H NMR (360 MHz, CDC13) 6 0.00 (9H, s, Me3Si), 0.90-0.95 (2H1, m, CH 2 ), 3.58-3.63 (2H, m,
CH
2 ), 4.84 (2H, s, CH 2 ), 5.80 (2H1, s, CH 2 ), 7.68 (1H, s, Ar-H). 15 c) 3-(5-Methylisoxazol-3-vl)-6-{ 1- [2-(trimethylsil1)ethoxy] methyl- 1,2,3 triazol-5-vyl}methyloxy- 1,2,4-triazolo[3,4-alphthalazine The title-compound was prepared from Intermediate 1 and the preceding alcohol following the procedure described for Example 10, 360 MHz (360 MHz, CDC13) 8 0.00 (9H, s, Me3Si), 0.88-0.93 (2H1, m, CH 2 ), 2.63 (3H, s, Me), 3.61-3.66 20 (2H, m, CH 2 ), 5.92 (2H1, s, CH 2 ), 5.97 (2H1, s, CH 2 ), 6.89 (1H1, s, Ar-H), 7.86 (1H, m, Ar-H), 8.02 (1H, t, J = 7.7 Hz, Ar-H), 8.18 (1H, s, Ar-H), 8.23 (1H1, d, J = 8.0 Hz, Ar-H), 8.76 (1H, d, J = 8.0 Hz, Ar-H); MS (ES+) m/e 479 [MH]+. d) 3-(5-Methylisoxazol-3-v1)-6-(1H-1,2,3-triazol-5-1l)methyloxy-1,2,4 triazolo [3,4-alphthalazine 25 A mixture of the preceding product, ethanol (10ml) and 2N HC1 (20ml) was heated at 50oC for 15.25h. The solution was basified to pH 12 with saturated sodium carbonate solution and the solvents evaporated in vacuo. The residue was azeotroped with ethanol (x2) and chromatographed on silica gel, eluting with 0-4% methanol/dichloromethane (gradient elution), to give the 30 title-product, 1H NMR (400 MHz, CDC1 3 ) 8 2.65 (3H1, s, Me), 5.73 (2H1, s, CH 2 ), 7.02 (1H, s, Ar-H), 7.87 (1H, t, J = 7.8 Hz, Ar-H), 7.99-8.03 (2H11, m, 2 of Ar-H), 8.24 (1H1, d, J = 8.2 Hz, Ar-H) 8.72 (1H, d, J = 7.9 Hz, Ar-H); MS (ES+) m/e 349
[MH]+.
WO 99/43677 - 10 - PCT/GB99/00485 EXAMPLE 1 3-(5-Methylisoxazol-3-yl)-6-(l1-methyl-1,2., 3 -triazol-5-yl)methyloxy-1,2,4 triazolo [3,4-alphthalazine, 3 -(5-methylisoxazol-3-vl)-6-(2-methyl-1,2,3-triazol-4 5 vyl)methyloxy-1,2,4-triazolof3,4-alphthalazine and 3-(5-methvlisoxazol-3-yl)-6-(1 methyl-1,2,3-triazol-4-yl)methyloxy- 1,2,4-triazolo [3,4-a]phthalazine Lithium hexamethyldisilazide (1.63ml of a 1M solution in THF, 1.63mmol) was added dropwise to a stirred solution of 3-(5-methylisoxazol-3-yl) 6-(1H-1,2,3-triazol-5-yl)methyloxy-1,2,4-triazolo[3,4-a]phthalazine (241mg, 10 0.626mmol) prepared as in Reference Example 3 in DMF (50ml) at -31oC under nitrogen. The mixture was warmed to -23oC over 1.5h, methyl iodide (0.10ml, 1.6mmol) added dropwise and the reaction mixture allowed to warm to room temperature overnight. Water was added and the solvent evaporated in vacuo. The residue was partitioned between dichloromethane and water and the 15 aqueous phase separated and re-extracted with dichloromethane (xl). The combined organic extrants were washed with brine (xl), dried (MgSO4) and evaporated in vacuo. Chromatography of the residue on silica gel, eluting with 0-5% methanol/dichloromethane (gradient elution), followed by preparative HPLC (YMC Sil column, 250 x 20mm) eluting with 5% methanol/l1-chlorobutane, 20 separated the triazole isomers: Least polar isomer (HPLC solvent system): 3-(5-methylisoxazol-3-vl)-6-(2 methyl-1,2,3-triazol-4-y1)methyloxy-1,2,4-triazolo[3,4-alphthalazine 1 H NMR (400 MHz, CDC1 3 ) 8 2.59 (3H, s, Me), 4.21 (3H, s, Me), 5.73 (2H, s, CH 2 ), 6.89 (1H, s, Ar-H), 7.79 (1H, m, Ar-H), 7.94 (1H1, m, Ar-H), 8.10 (1H, s, Ar-H), 8.22 25 (1H, d, J = 8.0 Hz, Ar-H), 8.67 (1H, d, J = 8.0 Hz, Ar-H); MS (ES+) m/e 363 [MH]+. Intermediate polarity isomer: 3-(5-methylisoxazol-3-vl)-6-(l1-methyl-1,2,3-triazol 4-yl)methyloxy-1,2,4-triazolo[3,4-alphthalazine 1 H NMR (400MHz, CDC13) 8 2.60 (3H1, s, Me), 4.09 (3H, s, Me), 5.78 (2H, s, CH 2 ), 6.90 (1H1, d, J = 0.8 Hz, Ar-H), 7.80 (1H, m, Ar-H), 7.94 (1H1, m, Ar-H), 8.25 (1H1, d, J = 8.0 Hz, Ar-H), 8.65 (11H, 30 d, J = 8.0 Hz, Ar-H), 8.73 (1H1, s, Ar-H); MS (ES+) m/e 363 [MH]+. Most polar isomer (HPLC solvent system): 3-(5-methylisoxazol-3-vl)-6-(l1-methyl 1,2,3-triazol-5-yl)methyloxy-1,2.,4-triazolof3.,4-alphthalazine 1H NMR (400 MHz, CDC1 3 ) 8 2.56 (3H1, s, Me), 4.19 (3H, s, Me), 5.76 (2H, s, CH2), 6.82 (1H, s, WO 99/43677 - 11 - PCT/GB99/00485 Ar-H), 7.80 (1H, m, Ar-H), 7.96 (1H, m, Ar-H), 8.04 (1H, s, Ar-H), 8.12 (1H, d, J = 8.8 Hz, Ar-H), 8.67 (1H, d, J = 8.0 Hz, Ar-H); MS (ES+) m/e 363 [MH]*.
Claims (5)
1. 3-(5-Methylisoxazol-3-yl)-6-(1-methyl-1,2,3-triazol-4-yl)methyloxy-1,2,4 5 triazolo[3,4-a]phthalazine.
2. A pharmaceutical composition comprising the compound as described in claim 1 and a pharmaceutically acceptable carrier. 10 3.
3-(5-Methylisoxazol-3-yl)-6-(1-methyl-1,2,3-triazol-4-yl)methyloxy-1,2,4 triazolo[3,4-a]phthalazine for use in a method of treatment of the human or animal body.
4. The use of the compound of claim 1 for the manufacture of a medicament 15 for the enhancement of cognition.
5. A method of enhancing cognition in a subject suffering from diminished cognition which comprises administering to that subject a cognition enhancing amount of the compound of claim 1.
Applications Claiming Priority (7)
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GBGB9803992.8A GB9803992D0 (en) | 1998-02-25 | 1998-02-25 | Therapeutic agents |
GB9803992 | 1998-02-25 | ||
PCT/GB1998/001307 WO1998050385A1 (en) | 1997-05-08 | 1998-05-06 | SUBSTITUTED 1,2,4-TRIAZOLO[3,4-a]PHTHALAZINE DERIVATIVES AS GABA ALPHA 5 LIGANDS |
GB98/01307 | 1998-05-06 | ||
GBGB9824896.6A GB9824896D0 (en) | 1998-11-12 | 1998-11-12 | Therapeutic compound |
GB9824896 | 1998-11-12 | ||
PCT/GB1999/000485 WO1999043677A1 (en) | 1998-02-25 | 1999-02-17 | SUBSTITUTED 1,2,4-TRIAZOLO[3,4-a]PYRIDAZINE |
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JP (1) | JP2002504554A (en) |
CN (1) | CN1291194A (en) |
AU (1) | AU746719B2 (en) |
BG (1) | BG104705A (en) |
CA (1) | CA2317416A1 (en) |
EA (1) | EA002824B1 (en) |
HR (1) | HRP20000555A2 (en) |
HU (1) | HUP0100647A3 (en) |
NZ (1) | NZ505695A (en) |
PL (1) | PL341975A1 (en) |
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US6465462B1 (en) * | 1996-07-25 | 2002-10-15 | Merck Sharp & Dohme Ltd. | Substituted triazolo pyridazine derivatives as inverse agonists of the GABAAα5 receptor subtype |
AU732455C (en) * | 1997-05-08 | 2002-09-05 | Merck Sharp & Dohme Limited | Substituted 1,2,4-triazolo{3,4-a}phthalazine derivatives as gaba alpha 5 ligands |
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EA002824B1 (en) | 2002-10-31 |
WO1999043677A1 (en) | 1999-09-02 |
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CN1291194A (en) | 2001-04-11 |
HUP0100647A2 (en) | 2001-09-28 |
EA200000878A1 (en) | 2001-02-26 |
EP1070066A1 (en) | 2001-01-24 |
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