AU2021105143A4 - A method and a device for quickly detecting the influence on the growth of duckweed - Google Patents
A method and a device for quickly detecting the influence on the growth of duckweed Download PDFInfo
- Publication number
- AU2021105143A4 AU2021105143A4 AU2021105143A AU2021105143A AU2021105143A4 AU 2021105143 A4 AU2021105143 A4 AU 2021105143A4 AU 2021105143 A AU2021105143 A AU 2021105143A AU 2021105143 A AU2021105143 A AU 2021105143A AU 2021105143 A4 AU2021105143 A4 AU 2021105143A4
- Authority
- AU
- Australia
- Prior art keywords
- duckweed
- growth
- culture
- growth rate
- control group
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 244000207740 Lemna minor Species 0.000 title claims abstract description 97
- 235000006439 Lemna minor Nutrition 0.000 title claims abstract description 95
- 235000001855 Portulaca oleracea Nutrition 0.000 title claims abstract description 95
- 230000012010 growth Effects 0.000 title claims abstract description 85
- 238000000034 method Methods 0.000 title claims abstract description 23
- 238000012360 testing method Methods 0.000 claims abstract description 54
- 230000000694 effects Effects 0.000 claims abstract description 20
- 239000002904 solvent Substances 0.000 claims abstract description 18
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 claims description 30
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 28
- 230000005764 inhibitory process Effects 0.000 claims description 18
- 238000002474 experimental method Methods 0.000 claims description 17
- 238000011081 inoculation Methods 0.000 claims description 11
- 238000009423 ventilation Methods 0.000 claims description 11
- 230000005855 radiation Effects 0.000 claims description 8
- 238000004458 analytical method Methods 0.000 claims description 7
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 6
- 229910052760 oxygen Inorganic materials 0.000 claims description 6
- 239000001301 oxygen Substances 0.000 claims description 6
- 239000001963 growth medium Substances 0.000 claims description 5
- 241001237160 Kallima inachus Species 0.000 claims description 3
- 230000035699 permeability Effects 0.000 claims description 3
- 229920006395 saturated elastomer Polymers 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 2
- 230000000149 penetrating effect Effects 0.000 claims description 2
- 231100000403 acute toxicity Toxicity 0.000 description 6
- 230000007059 acute toxicity Effects 0.000 description 6
- 238000010586 diagram Methods 0.000 description 6
- 238000001514 detection method Methods 0.000 description 3
- 230000009036 growth inhibition Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 229930002875 chlorophyll Natural products 0.000 description 2
- 235000019804 chlorophyll Nutrition 0.000 description 2
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 239000012780 transparent material Substances 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 1
- 240000000067 Spirodela polyrhiza Species 0.000 description 1
- 235000014249 Spirodela polyrhiza Nutrition 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 231100000215 acute (single dose) toxicity testing Toxicity 0.000 description 1
- 231100000369 acute toxicity data Toxicity 0.000 description 1
- 238000011047 acute toxicity test Methods 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000002015 leaf growth Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0098—Plants or trees
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Wood Science & Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The technical scheme of the disclosure provides a method and a device for
quickly detecting the influence on the growth of duckweed. The method comprises:
providing a concentration test group, a solvent control group and a blank control
group, wherein the concentration test group comprises at least three first culture
solutions, the solvent control group comprises at least one second culture solution
and the blank control group comprises at least one third culture solution; the first
culture solution, the second culture solution and the third culture solution are
respectively injected into a plurality of culture containers at the temperature of
24±2°C; the inoculated culture containers are randomly placed under the light
source and the growth state of duckweed leaves was observed; and taking the leaf
area of duckweed as the effect index, the effect of foreign aid on the growth of
duckweed was detected.
1/3
300
| W
200
100 110
FIG. 1
110
113
Cl Co Co C 111
FIG. 2
Description
1/3
300
| W
200
100 110 FIG. 1
110 113
Cl Co Co C 111
FIG. 2
[0001] This disclosure claims priority to Chinese Application number 202110853796.5, filed on July 28, 2021.
[0002] The disclosure relates to the field of biological detection, in particular to a method and a device for quickly detecting the influence on the growth of duckweed.
[0003] Lemnaceae duckweed is the representative of higher aquatic duckweed, which is not only a water producer, but also a shelter and habitat for small invertebrates, and plays an important role in aquatic ecosystem. Foreign toxicologists have proposed to use duckweed for toxicity test. Compared with algae test, which is often used to detect the toxicity of pollutants, duckweed test has relatively loose requirements for aseptic operation, simple counting and simple operation. Duckweed is a better test organism.
[0004] Therefore, duckweed growth inhibition test has attracted wide attention and has been gradually applied to acute toxicity test of pesticides and environmental monitoring.
[0005] The technical problem to be solved by the disclosure is to provide a method and a device for quickly detecting the influence on duckweed growth, which may quickly and accurately determine the influence of foreign aids on duckweed growth.
[0006] According to some aspects of the present disclosure, a method for quickly detecting the influence on duckweed growth is provided. The method comprises: providing a concentration test group, a solvent control group and a blank control group, where the concentration test group comprises at least three first culture solutions which include phenol with a concentration gradient and acetone with the same concentration, the solvent control group comprises at least one second culture solution which includes acetone but not phenol, the blank control group comprises at least one third culture solution which includes no acetone and phenol; the first culture solution, the second culture solution and the third culture solution are respectively injected into a plurality of culture containers at the temperature of 24±20 C, and carry out selective sterilization according to the culture target; duckweed is inoculated into each culture container under aseptic conditions, and the opening of the culture container is closed while ensuring air permeability; the inoculated culture containers are randomly placed under the light source, the duckweed grows for a specific time under specific light-dark time ratio and the growth state of duckweed leaves was observed; and taking the leaf area of duckweed as the effect index, the effect of foreign aid on the growth of duckweed was detected.
[0007] According to some aspects of the present disclosure, a device for quickly detecting the influence on the growth of duckweed is provided. The device comprising: a culture container group which comprises a plurality of culture containers serving as a concentration test group, a solvent control group and a blank control group, the culture containers comprise a transparent body and a cover plate for covering the top opening of the transparent body, and the cover plate at least comprises two ventilation through holes penetrating through the cover plate; a light source loading device comprises at least one group of supporting frames and top shelf, the supporting frames are positioned on opposite sides of the plurality of culture containers, and the top shelf are correspondingly configured with the supporting frames, and span over the culture containers and are connected with the supporting frames; a light source assembly comprises a luminous source and a reflector, the top of the reflector is suspended on the top shelf and positioned above the culture container, the bottom of the reflector is provided with an opening, the luminous source is arranged in the reflector, the inner wall of the reflector also comprises Z-shaped fold; and a timing switch device that controls the operating state of the luminous source.
[0008] According to the technical scheme of the disclosure, the method and device for quickly detecting the influence on the growth of duckweed may fully meet the requirements on the growth of duckweed. The quickly detecting method is easy to observe the influence on duckweed after the foreign aid is added into the culture solution, which may be qualitatively observed or quantitatively measured. The quickly detecting method also meets the requirements of relevant national environmental safety evaluation test criteria and water quality monitoring.
[0009] According to the technical scheme of the disclosure, the method for quickly detecting the influence of duckweed growth breaks the set thinking that thallus are used as the test index in the industry. Leaf area is selected as the test index for the influence of duckweed growth. The defect that the current detection method needs to span generations is solved. The method of the disclosure greatly shortens the detection time of duckweed growth inhibition test to 48 hours, saves 5 days compared with the prior art, thus breaking the technical barrier and laying a foundation for further discussing the influence of other foreign aids on duckweed.
[0010] According to the technical scheme of the disclosure, the device for quickly detecting the influence on the growth of duckweed is simple in structure and easy to operate. The duckweed may grow stably under the condition of manual control. The device of the disclosure provides a foundation for quickly detecting of duckweed growth.
[0011] In addition, the above summary does not enumerate all the essential features of the present disclosure. In addition, sub-combinations of these feature groups may also constitute inventions.
[0012] In order to explain the technical solutions in the disclosure, the following will briefly introduce the drawings needed in the embodiment description. Obviously, the drawings in the following description are only some exemplary embodiments of the disclosure. For those skilled in the art, other drawings may be obtained according to these drawings without creative labor.
[0013] FIG. 1 is a structural schematic diagram of a device for quickly detecting the influence on duckweed growth according to an embodiment of the present disclosure;
[0014] FIG. 2 is a schematic structural diagram of a culture container according to an embodiment of the present disclosure;
[0015] FIG. 3 is a schematic structural diagram of a light source loading device according to an embodiment of the present disclosure;
[0016] FIG. 4 is a schematic structural diagram of a light source assembly according to an embodiment of the present disclosure;
[0017] FIG. 5 is a schematic view of the structure of the light source assembly viewed along the P direction of FIG. 4;
[0018] FIG. 6 is a schematic structural diagram of an inoculation ring according to an embodiment of the present disclosure.
[0019] The following description provides the specific disclosure scenarios and requirements of this disclosure in order to enable those skilled in the art to make or use the contents of this disclosure. Various modifications to the disclosed embodiments will be apparent to those skilled in the art, and the general principles defined herein may be applied to other embodiments and s without departing from the scope of this disclosure. Therefore, this disclosure is not limited to the illustrated embodiments, but is to be accorded the widest scope consistent with the claims.
[0020] At present, in the study of duckweed growth inhibition experiments, the thallus number or chlorophyll of duckweed is selected as the test index. However, the thallus number is easily affected by other factors in short-term experiments, the test results are not obvious. The determination procedure of chlorophyll index is complex, the test results may not be obtained quickly.
[0021] The inventor of this disclosure has made an in-depth study on the inhibition test of duckweed. The inventor found that when the leaf area of duckweed is taken as the test index, the growth change of duckweed may be obtained in a very short time, and then the influence of foreign aid on the growth of duckweed may be quickly and accurately judged. The device for quickly detecting the growth influence of duckweed provided by this disclosure may make duckweed grow stably under the condition of manual control, and further improve the accuracy of the test results.
[0022] As shown in FIG. 1, the device for quickly detecting the influence of duckweed growth in the embodiment of the disclosure comprises: a culture container group 100, a light source loading device 200, a light source assembly 300 and a timing switch device (not shown). The culture container group 100 is located below the light source assembly 300. The culture container group 100 includes several culture containers 110 as a concentration test group, a solvent control group and a blank control group. The number of the culture containers 110 is determined according to the test requirements. FIG. 1 shows three of the culture containers 110. The light source loading device 200 is used for fixing the light source assembly 300, and the light source assembly 300 provides a light source for duckweed growth.
[0023] FIG. 2 is a schematic structural diagram of the culture container 110. The culture container 110 includes a transparent body 111 and a cover plate 112. The transparent body 111 may be a container made of transparent material. For example, the transparent body 111 may be a transparent glass container. The container made of transparent material is convenient to observe the influence of foreign aids on the leaf area of duckweed. The outer wall or inner wall of the transparent body 111 may be provided with a volume scale, and the outer wall of the transparent body 111 may be provided with an electronic thermometer and an electronic hygrometer. The wall thickness of the transparent body 111 is preferably 2mm.
[0024] The cover plate 112 is used to cover the top opening of the transparent body 111, and the cover plate 112 includes at least two ventilation through holes 113 penetrate through the cover plate 112. The diameter of the ventilation through holes 113 and the density of the ventilation through holes 113 on the cover plate 112 directly affect the test. As at least one parameter of the diameter or density of the ventilation through holes 113 decreases, it is more beneficial to maintain the sterile environment in the culture container 110, but it is not conducive to the ventilation effect required by duckweed growth. In this embodiment, the diameter of the ventilation through holes 113 is 3mm-5mm, and the density of the ventilation through holes 113 on the cover plate 112 is 0.2/cm 2-0.3/cm 2. The culture container 110 may not only meet the sterile environment required by the test, but also make the duckweed grow normally.
[0025] As shown in FIG. 3, the light source loading device 200 includes at least one group of supporting frames 210 and top shelfs 220. The supporting frames 210 are arranged on opposite sides of the plurality of culture containers 110. The top shelfs 220 are arranged corresponding to the supporting frames 210. The top shelfs 220 span over the culture containers 110 and are connected with the supporting frames 210.
[0026] As shown in FIG. 4, the light source assembly 300 includes a luminous source 310 and a reflector 320, where the luminous source 310 is arranged in the reflector 320, and the luminous source 310 provides a light source necessary for duckweed growth. The top of the reflector 320 is suspended from the top shelf 220 and located above the culture container 110, and the bottom of the reflector 320 has an opening. The reflector 320 may reflect the scattered light, which enables the light to be concentrated toward the culture container 110, and make full use of the light energy provided by the luminous source 310. In this embodiment, the reflector 320 has a triangular prism shape, which has the best focusing effect. In other embodiments, the reflector 320 may also take other shapes, such as quadrangular prism, hemispherical shape, etc.
[0027] As shown in FIG. 5, the structure of the light source assembly 300 is viewed along the P direction of FIG. 4 (that is, viewed upward from the bottom opening of the reflector 320). The inner wall of the reflector 320 includes a Z-shaped fold 321. In some embodiments, the Z-shaped fold 321 is located on the inner wall of the plane where the long side lies. The apex angle of the Z-shaped fold 321 is 130 140 degrees, which has the best focusing effect. A distance between the luminous source 310 and the culture container 110 is 50cm-100cm, and a radiation wavelength of the light source 310 is 400nm-700nm, and a radiation light intensity is 65001x-1000O0x.
[0028] The timing switch device is used to control the running state of the luminous source 310. In this embodiment, the timing switch device controls the luminous source 310 to run according to the light-dark time ratio of 16: 8, that is, it is turned on for 16 hours during the day and turned off for 8 hours at night. In some embodiments, the timing switch device is electrically or communicatively connected with the luminous source 310.
[0029] As shown in FIG. 6, the quickly detecting device of the embodiment of the present disclosure further includes an inoculation ring 400. The inoculation ring 400 is used for inoculating duckweed into the transparent body 111. The inoculation ring
400 comprises an inoculation ring loop 410 and an inoculation ring handle 420 which are connected.
[0030] The embodiment of the disclosure also provides a method for quickly detecting the influence on the growth of duckweed, which uses the quickly detecting device above. The method comprises the following steps.
[0031] Step S1: providing a concentration test group, a solvent control group and a blank control group. The concentration test group comprises at least three first culture solutions including phenol with a concentration gradient and acetone with the same concentration. The solvent control group comprises at least one second culture solution including acetone but not phenol. The acetone concentration of the second culture solution is equal to that of the first culture solution. The blank control group comprises at least one third culture solution including no acetone and phenol.
[0032] Step S2: respectively injecting the first culture solution, the second culture solution and the third culture solution into a plurality of culture containers at the temperature of 24±2°C, and carry out selective sterilization according to the culture target.
[0033] Step S3: inoculating duckweed into each culture container under aseptic condition, and closing the opening of the culture container while ensuring air permeability.
[0034] Step S4: randomly placing the inoculated culture container under the light source, growing duckweed for a specific time under a specific light-dark time ratio, and observing the growth state of duckweed leaves.
[0035] Step S5, taking the leaf area of duckweed as an effect index, detecting the influence of foreign materials on the growth of duckweed.
[0036] In step S2, the duckweed inoculated in the culture container is cultured in duckweed growth medium for at least 8 weeks in advance, and the doubling time of the number of thallus during the culture is within 60 hours.
[0037] In step S4, the radiation wavelength of the light source is 400nm-700nm, the radiation light intensity is 65001x-100001x, and the light-dark time ratio is controlled to be 16: 8. Observing the growth state of duckweed leaves includes: measuring dissolved oxygen, pH value, temperature and phenol concentration of the concentration test group at regular intervals, and keeping the temperature at 24±2 °C, pH value at 7.1±0.3, the mass of dissolved oxygen exceeding 80% of saturated solubility, and the fluctuation range of phenol concentration not exceeding±20%, cleaning dead leaf bodies in time, and observing and recording leaf areas at the beginning and end of the test.
[0038] In step S5, taking the leaf area of duckweed as the effect index and the influence of foreign aid on the growth of duckweed was detected includes:
[0039] Step S51, taking leaf area as the effect index, the initial leaf area and the end leaf area are calculated by graphic analysis;
[0040] Step S52, according to the initial leaf area and the end leaf area, the growth rate of duckweed in the concentration test group, the solvent control group and the blank control group is obtained as follows:
In Mz 2 - In Mz 1 U- Uz t where, U2 is the growth rate of the tested organism in experimental container z, its unit is per day; Mza is the effect index of the calculated growth rate of the tested organism at the beginning of the experiment in experimental container z; Mz,2 is the effect index of the calculated growth rate of the tested organism at the end of the experiment in experimental container z; tz is the interval between the beginning and the end of the experiment in experimental container z, its unit is day; z is a certain experimental container for experiment which is dimensionless;
[0041] Step S53, according to the growth rate of duckweed, the influence of foreign aid on the growth of duckweed is analyzed.
[0042] In an embodiment of the present disclosure, detecting the influence of foreign aid on the growth of duckweed comprises:
[0043] obtaining an inhibition rate of growth rate according to the growth rate of the duckweed, the inhibition rate of growth rate is obtained as follows:
V2=UO UZ x 100% U0
where, V2 is the inhibition rate of growth rate of the tested organism in the
experimental container z, its unit is %; Uo is the arithmetic average of the growth rate of the tested organism in the blank control group, its unit is per day; Uz is the growth rate of the tested organism in the experimental container z, its unit is per day; and z is a certain experimental container for experiment which is dimensionless;
[0044] obtaining an average inhibition rate of growth rate according to the growth rate inhibition rate, the average inhibition rate of growth rate is obtained as follows:
V = v U" -U X lO 100%
where, V, is the average inhibition rate of growth rate of tested organisms in
concentration test group c, its unit is %; Uo is the arithmetic average of growth rate
of tested organisms in blank control group, it unit is per day; Uc is the arithmetic average of growth rate of tested organisms in concentration test group c, its unit is per day; and c is a certain concentration test group which is dimensionless;
[0045] performing significant difference analysis on the average inhibition rate of growth rate.
[0046] The quickly detecting method of the embodiment of this disclosure is described in detail by specific examples below.
[0047] The selected test substance is phenol (analytically pure). The tested duckweed is Spirodelapolyrrhiza, which has 12 thallus and the initial total area is 85 ±5mm2 .
[0048] Before the start of the experiment, the duckweed plants were cultured in the duckweed growth medium for at least 8 weeks, and the doubling time of the number of thallus during the culture period was about 60 hours.
[0049] The experimental water is sterilized at 120°C for 15min, which is used as culture medium. A concentration test group, a solvent control group and a blank control group are provided. The concentration test group comprises five groups of first culture solutions including acetone and phenol. The concentrations of acetone are all 0.1mg/L. The concentrations of phenol are 400.mg/L, 800.mg/L, 1600.Omg/L, 3200.Omg/L and 6400.Omg/L respectively. The solvent control group comprises a group of second culture solutions including 0.Omg/L phenol and 0.1mg/L acetone. The blank control group comprises a third culture solution including no phenol and acetone. Each culture medium mentioned above is provided with three repeated experiments, randomly numbered #1, #2 and #3. Repeated experiments with the same number form a parallel group, which consists of three parallel groups, numbered as parallel group A, parallel group B and parallel group C in turn.
[0050] Preparing 21 culture containers with a volume of 250mL each, and add 150mL of prepared culture solution respectively. Under aseptic conditions, 12 thallus (3 clones) are inoculated into the transparent body of the culture container by using the inoculation ring. The inoculated culture container was randomly placed under the light source assembly. A timing switch device is adopted to control the light source assembly to operate according to the light-dark time ratio of 16: 8. The radiation wavelength of the light source is 400nm-700nm, and the radiation light intensity is 65001x-1000O0x.
[0051] During the test, the dissolved oxygen, pH value, temperature and phenol concentration of each concentration test group ware measured every 24 hours. The solution temperature is kept at 24i2°C, solution pH value is kept at 7.1-0.3, the mass of dissolved oxygen of the solution exceeded 80% of saturated solubility, and the fluctuation range of phenol concentration does not exceed 20%. The dead leaf bodies are cleaned in time, and the leaf area is observed and recorded at the beginning and end of the test, and the test is exposed for 48 hours.
[0052] Taking leaf area as an effect index, the initial leaf area and the end leaf area are calculated by graphic analysis using computer software. The growth rates of duckweed in the concentration test group, solvent control group and blank control group are obtained through the initial leaf area and end leaf area. According to the growth rate of duckweed, the influence of foreign aids on duckweed growth is analyzed.
[0053] Table 1 shows the 48-hour acute toxicity results of phenol to duckweed. Table 2 shows the distribution test results of the 48-hour growth rate acute toxicity experimental data of phenol to duckweed. Combined with Table 1 and Table 2, the 48-hour growth rate of duckweed in three parallel groups accords with normal distribution, satisfies homogeneity of variance, and has no significant difference among parallel groups, which satisfies the condition of linear fitting for phenol acute toxicity data of duckweed in 48 hours.
[0054] Table 1: Experimental results of acute toxicity of phenol to duckweed Average Initial leaf End leaf Growth inhibition Test group Repeat area area (mm 2 rate rate of (mm 2 ) ) (d-1) growth rate (%) Blank #1 88 114 0.13 control group #2 86 114 0.14
#3 86 113 0.14
#1 84 110 0.13 Solvent control #2 84 110 0.13 0.59 group #3 84 110 0.13
#1 82 103 0.11
400.Omg/L #2 82 104 0.12 11.72
#3 80 103 0.13
#1 80 100 0.11
800.Omg/L #2 82 99 0.09 29.24
#3 84 99 0.08
#1 84 95 0.06
1600.Omg/L #2 84 96 0.07 53.35
#3 84 95 0.06
#1 86 88 0.01
3200.Omg/L #2 86 89 0.02 91.54
#3 86 87 0.01
#1 88 86 -0.01
6400.Omg/L #2 88 85 -0.02 111.35
#3 88 85 -0.02
[0055] Table 2: Distribution test results of experimental data of acute toxicity of phenol to the growth rate of duckweed
Content Test result Parallel p = 0.1165 group A Normal Parallel p = 0.4196 distribution test group B Parallel p = 0.3072 group C Homogeneity test of variance F = 0.0224, p > 0.05 between parallel groups Parallel q = 0.0627, p > 0.05 Analysis of mean group A/B difference Parallel q = 0.0627, p > 0.05 between parallel group A/C groups Parallel q = 0.1254, p > 0.05 group B/C q
[0056] The variance analysis of the growth rate between the blank control group and the solvent control group showed no significant difference (a.=0.05, p=0.91 1) The ECsoof the 48-hour acute toxicity experiment of phenol to duckweed is calculated by the blank control group, and the ECso results are shown in Table 3.
[0057] Table 3: Acute toxicity of phenol to duckweed
48h-ECs (95% CI) Water Water Fitting Fig ,2 (temperature pH equation (phenol, tg/L) (oC) value 1.246x106 y486.888x- 0.9842 <0.01 7.205x1062.383x10 24 7.1 479.630 7.05 24.37.1
[0058] Under the effective experimental conditions, the P values of the leaf area of the experimental group and the control group with different concentrations of phenol are all greater than 0.05 at the level of 0.05, indicating that there is no significant difference between the experimental group and the control group. The
48h-ECso of phenol to duckweed is 1.247 X 106 1 g/L, and the 95%C is 7.205 X 106~2.383 X 106 11g/L. During the experiment, there is no other abnormality between the experimental group and the control group.
[0059] In summary, after reading this detailed disclosure, those skilled in the art may understand that the foregoing detailed disclosure may be presented by way of example only and may not be restrictive. Although not explicitly stated here, those skilled in the art will understand that this disclosure is intended to cover various reasonable changes, improvements and modifications to the embodiments. These changes, improvements and modifications are intended to be proposed by this disclosure and are within the spirit and scope of the exemplary embodiments of this disclosure.
[0060] In addition, some terms in this disclosure have been used to describe embodiments of this disclosure. For example, "one embodiment", "an embodiment" and/or "some embodiments" mean a particular feature, structure or characteristic described in connection with the embodiment, which may be included in at least one embodiment of the present disclosure. Therefore, it may be emphasized and understood that two or more references to "embodiment" or "one embodiment" or "alternative embodiment" in various parts of this disclosure do not necessarily all refer to the same embodiment. In addition, specific features, structures, or characteristics may be appropriately combined in one or more embodiments of this specification.
[0061] It should be understood that in the foregoing description of the embodiments of the present disclosure, in order to help understand one feature and for the purpose of simplifying the present disclosure, the present disclosure combines various features in a single embodiment, drawings or descriptions thereof. However, this does not mean that the combination of these features is necessary, and it is entirely possible for those skilled in the art to extract some of the features as separate embodiments when reading this specification. That is to say, the embodiments in this disclosure may also be understood as the integration of multiple sub-embodiments. However, the content of each sub-embodiment is also true when it is less than all the features of a single previously disclosed embodiment.
[0062] Each patent, patent disclosure, publication of patent disclosure and other materials cited herein, such as articles, books, specifications, publications, documents, articles, etc., may be incorporated herein by reference. All contents used for all purposes, except any history of prosecution documents related to them, may be inconsistent or conflict with this document, or any same history of prosecution documents that may have a restrictive effect on the broadest scope of claims. Are now or later associated with this document. For example, if there is any inconsistency or conflict between the description, definition and/or use of terms associated with any included materials and those related to this document, the terms in this document shall prevail.
[0063] Finally, it should be understood that the embodiments of the disclosure disclosed herein are illustrative of the principles of the embodiments of the disclosure. Other modified embodiments are also within the scope of this disclosure. Therefore, the embodiments disclosed in this disclosure are merely examples and not limitations. Those skilled in the art may adopt alternative configurations according to the embodiments in this disclosure to realize the technical scheme in this disclosure. Therefore, the embodiments of this disclosure are not limited to the embodiments accurately described in this disclosure.
Claims (10)
1. A method for quickly detecting the influence on the growth of duckweed, comprising: providing a concentration test group, a solvent control group and a blank control group, wherein the concentration test group comprises at least three first culture solutions which comprise phenol with a concentration gradient and acetone with the same concentration, the solvent control group comprises at least one second culture solution which comprises acetone but not phenol, the blank control group comprises at least one third culture solution which comprises no acetone and phenol; the first culture solution, the second culture solution and the third culture solution are respectively injected into a plurality of culture containers at the temperature of 24±2C and carry out selective sterilization according to the culture target; duckweed is inoculated into each culture container under aseptic conditions, and the opening of the culture container is closed while ensuring air permeability; the inoculated culture containers are randomly placed under the light source, the duckweed grows for a specific time under specific light-dark time ratio and the growth state of duckweed leaves was observed; and taking the leaf area of duckweed as the effect index, the effect of foreign aid on the growth of duckweed was detected.
2. The method according to claim 1, wherein, the duckweed inoculated in the culture container is cultured in duckweed growth medium for at least 8 weeks in advance, and the doubling time of the number of thallus during the culture period is within 60 hours.
3. The method according to claim 1, wherein, observing the growth state of duckweed leaves comprises: measuring dissolved oxygen, pH value, temperature and phenol concentration of the concentration test group at regular intervals, and keeping the temperature at 24±2C, the pH value at 7.1±0.3, the mass of dissolved oxygen exceeding 80% of saturated solubility, the fluctuation range of phenol concentration not exceeding ±20%, cleaning dead leaf bodies in time, and observing and recording leaf areas at the beginning and end of the test.
4. The method according to claim 1, wherein, taking the leaf area of duckweed as the effect index, the influence of foreign aid on the growth of duckweed was detected, comprises: taking leaf area as the effect index, the initial leaf area and the end leaf area are calculated by graphic analysis; according to the initial leaf area and the end leaf area, the growth rate of duckweed in the concentration test group, the solvent control group and the blank control group is obtained as follows:
In Mz, - In Mz, I
z tz
Wherein, Uz is the growth rate of the tested organism in experimental container z, and its unit is per day; Mzj is the effect index of the calculated growth rate of the tested organism at the beginning of the experiment in experimental container z;Mz,2 is the effect index of the calculated growth rate of the tested organism at the end of the experiment in experimental container z; tz is the interval between the beginning and the end of the experiment in experimental container z, its unit is day; z is a certain experimental container for experiment which is dimensionless; and according to the growth rate of duckweed, the influence of foreign aid on the growth of duckweed is analyzed.
5. The method according to claim a, wherein, detecting the influence of foreign aid on the growth of duckweed comprises: obtaining a inhibition rate of growth rate according to the growth rate of the duckweed, the inhibition rate of growth rate is obtained as follows:
Vz=UO U x 100% 0.
wherein, V2 is the inhibition rate of growth rate of the tested organism in the
experimental container z, its unit is %; Uo is the arithmetic average of the growth rate of the tested organism in the blank control group, its unit is per day; Uz is the growth rate of the tested organism in the experimental container z, its unit is per day; and z is a certain experimental container for experiment which is dimensionless; obtaining an average inhibition rate of growth rate according to the growth rate inhibition rate, the average inhibition rate of growth rate is obtained as follows:
V =UU x 100% wherein, Vc is the average inhibition rate of growth rate of tested organisms in
concentration test group c, its unit is %; Uo is the arithmetic average of growth rate of tested organisms in blank control group, it unit is per day; U is the arithmetic average of growth rate of tested organisms in concentration test group c, its unit is per day; and c is a certain concentration test group which is dimensionless; and performing significant difference analysis on the average inhibition rate of growth rate.
6. The method according to claim 1, wherein, the radiation wavelength of the light source is 400nm-700nm, the radiation light intensity is 65001x-1000O1x, and the light-dark time ratio is 16: 8.
7. A device for quickly detecting the influence on the growth of duckweed, comprising: a culture container group which comprises a plurality of culture containers serving as a concentration test group, a solvent control group and a blank control group, the culture containers comprise a transparent body and a cover plate for covering the top opening of the transparent body, and the cover plate at least comprises two ventilation through holes penetrating through the cover plate; a light source loading device comprises at least one group of supporting frames and top shelf, the supporting frames are positioned on opposite sides of the plurality of culture containers, and the top shelf are correspondingly configured with the supporting frames, and span over the culture containers and are connected with the supporting frames; a light source assembly comprises a luminous source and a reflector, the top of the reflector is suspended on the top shelf and positioned above the culture container, the bottom of the reflector is provided with an opening, the luminous source is arranged in the reflector, the inner wall of the reflector also comprises Z shaped fold; and a timing switch device that controls the operating state of the luminous source.
8. The device according to claim 7, wherein, the distance between the luminous source and the culture container is 50cm-100cm, the apex angle of the Z shaped fold is 130-140 degrees.
9. The device according to claim 7, wherein, the diameter of the ventilation through holes is 3mm-5mm, the density of the ventilation through holes on the cover plate is 0.2-0.3 /cm2 .
10. The device according to claim 7, further comprises an inoculation ring for inoculating duckweed into the transparent body, and the inoculation ring comprises an inoculation ring loop and an inoculation ring handle which are connected.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110853796.5 | 2021-07-28 | ||
| CN202110853796.5A CN113341078B (en) | 2021-07-28 | 2021-07-28 | Method and device for rapidly detecting influence on growth of duckweed |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2021105143A4 true AU2021105143A4 (en) | 2021-10-07 |
Family
ID=77480429
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2021105143A Ceased AU2021105143A4 (en) | 2021-07-28 | 2021-08-09 | A method and a device for quickly detecting the influence on the growth of duckweed |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN113341078B (en) |
| AU (1) | AU2021105143A4 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114280285B (en) * | 2021-12-30 | 2022-06-21 | 生态环境部南京环境科学研究所 | Device for testing acute toxicity of rice disease and pest control agent to shellfish aquatic organisms |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103849550B (en) * | 2014-03-20 | 2015-09-16 | 环境保护部南京环境科学研究所 | A kind of algae secondary culture apparatus and cultural method thereof |
| CN104017726A (en) * | 2014-06-30 | 2014-09-03 | 张玟籍 | Built-in light source bioreactor and microalgae culture method |
| CN105454026A (en) * | 2015-11-19 | 2016-04-06 | 中国科学院青岛生物能源与过程研究所 | Duckweed stereoscopic culturing apparatus |
| US20200060108A1 (en) * | 2018-03-27 | 2020-02-27 | Energy Management Concepts, Inc. | Indoor process and system for cultivating and harvesting duckweed |
| CN112413540A (en) * | 2020-12-07 | 2021-02-26 | 常州市金流明光电科技有限公司 | Adjustable reflecting cover for plant illuminating lamp |
-
2021
- 2021-07-28 CN CN202110853796.5A patent/CN113341078B/en active Active
- 2021-08-09 AU AU2021105143A patent/AU2021105143A4/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| CN113341078A (en) | 2021-09-03 |
| CN113341078B (en) | 2021-12-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4559299A (en) | Cytotoxicity assays in cell culturing devices | |
| Witty et al. | Direct evidence for changes in the resistance of legume root nodules to O2 diffusion | |
| O'Riordan et al. | A cell viability assay based on monitoring respiration by optical oxygen sensing | |
| Goldman et al. | Nonlinear time-course uptake of carbon and ammonium by marine phytoplankton | |
| Burggren | Gas exchange, metabolism, and" ventilation" in gelatinous frog egg masses | |
| CN106520541B (en) | A kind of kit of zebrafish embryo acute toxicity testing | |
| BR112012027837A2 (en) | carbon dioxide detection device, method of producing a carbon dioxide detection device, carbon dioxide detection method, gas detection device produced as a metabolic product of a microorganism, and detection method of a gas produced as a metabolic product of a microorganism | |
| AU2021105143A4 (en) | A method and a device for quickly detecting the influence on the growth of duckweed | |
| US3694317A (en) | Method of and instrument for micro-biological analysis | |
| Jen et al. | The antibacterial activity of alamethicins and zervamicins | |
| Gao et al. | Measurement of benthic photosynthesis and calcification in flowing‐through seawater with stable carbonate chemistry | |
| CN103308361A (en) | Chromosome slide preparing method | |
| CN206545021U (en) | A kind of kit of zebrafish embryo acute toxicity testing | |
| CN105388263B (en) | The method evaluated using microbial food web efficiency shallow lake fisheries environment | |
| JPWO2010087110A1 (en) | Microorganism automatic analyzer and microorganism automatic analysis method | |
| CN100554408C (en) | Extracorporeal blood vessel regenerating model and the application in biomaterial vascularization functional evaluation thereof | |
| Quesnel | Chapter X Methods of Microculture | |
| Hohenegger et al. | Growth of the foraminifer Nummulites venosus in nature and culture | |
| Ammann et al. | Gas Exchange of Algae: III. Relation between the Concentration of Carbon Dioxide in the Nutrient Medium and the Oxygen Production of Chlorella pyrenoidosa | |
| CN108956429A (en) | A kind of river inkstone filtration rate rapid assay methods based on flow cytometry | |
| US20050026135A1 (en) | Method for rapid detection of microorganisms by changing the shape of micro colonies | |
| CN102524178A (en) | Screening method of febrile convulsion model rat and application thereof | |
| Hill | “Hanging block” preparations for the microscopic observation of developing bacteria | |
| Peng et al. | Microcalorimetric studies of the action of Er 3+ on Halobacterium halobium R1 growth | |
| Jiang et al. | Advanced Single Cell Imaging Method Using Microfluidics and FUCCI to Determine Cell Cycle Progression and Cell Fate Decisions |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGI | Letters patent sealed or granted (innovation patent) | ||
| MK22 | Patent ceased section 143a(d), or expired - non payment of renewal fee or expiry |