AU2021104907A4 - Microbial Bed with Temperature Regulation - Google Patents

Microbial Bed with Temperature Regulation Download PDF

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Publication number
AU2021104907A4
AU2021104907A4 AU2021104907A AU2021104907A AU2021104907A4 AU 2021104907 A4 AU2021104907 A4 AU 2021104907A4 AU 2021104907 A AU2021104907 A AU 2021104907A AU 2021104907 A AU2021104907 A AU 2021104907A AU 2021104907 A4 AU2021104907 A4 AU 2021104907A4
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AU
Australia
Prior art keywords
box body
bolts
fixedly connected
fixed cylinders
base
Prior art date
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Ceased
Application number
AU2021104907A
Inventor
Yu He
Kaiwen Wang
Shuai WANG
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Xuzhou University of Technology
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Xuzhou University of Technology
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Priority to AU2021104907A priority Critical patent/AU2021104907A4/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • C12M1/36Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
    • C12M1/38Temperature-responsive control
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • C12M1/10Apparatus for enzymology or microbiology rotatably mounted

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a microbial bed with temperature regulation, which comprises a sealing cover, a box body and a base. The upper surface of the base is fixedly connected with the box body through bolts. The box body is plugged with the sealing cover, and the box body and the sealing cover slidably connect. The invention is provided with fixed cylinders, a gear and a motor, and the motor drives the rotating rod to rotate, thereby driving the gear to rotate, so as to drive two fixed cylinders to rotate. On the one hand, by rotating the fixed cylinders, the culture medium in the fixed cylinders can be heated uniformly, so that the conditions of microbial culture will not change too much, ensuring that the cultivated microbes meet the requirements of screening. On the other hand, during the rotation of the fixed cylinders, the sewage in the fixed cylinders can be thrown out through the action of centrifugal force, and then the sewage in the culture medium can be discharged, and can be discharged from the box body through the through holes and the drain pipe to avoid the contamination of the culture conditions during the microorganism culture process. 2/3 Figure 2 Figure 3

Description

2/3
Figure 2
Figure 3
Microbial Bed with Temperature Regulation
TECHNICAL FIELD
The invention relates to the technical field of microbial beds, in particular to a microbial
bed with temperature regulation.
BACKGROUND
Microbial culture is a kind of biological culture. The cultured microorganisms mainly
include viruses, bacteria, actinomycetes, fungi, etc. The medium in microbial culture is a
mixture of required nutrients and is artificially prepared in a certain proportion for the
growth and reproduction of microorganisms and the synthesis of metabolites. The raw
materials of the culture medium can be divided into carbon sources, nitrogen sources,
inorganic salts, growth factors and water. According to the types of microorganisms and
experimental purposes, the culture medium also has different types and preparation
methods. However, there are some shortcomings in the microbial bed of microorganisms
culture, such as:
In the prior art, when the microbial bed is cultured for microorganisms, the microbial
culture medium is placed in the culture bed, which can easily cause uneven heating, and
the cultivated microorganisms do not meet the screening requirements due to different
culture conditions.
SUMMARY
The object of the present invention is to provide a microbial bed with temperature
regulation to solve the problems raised in the background part.
The present invention provides the following technical solutions:
A microbial bed with temperature regulation, comprising a sealing cover, a box body and
a base, wherein the upper surface of the base is fixedly connected with the box body by
bolts. The box body is plugged with the sealing cover, and the box body is slidably
connected with the sealing cover. The box body includes two fixed cylinders, a gear and a
water inlet pipe. A bracket is fixedly connected to the lower surface of the inner wall of
the base through bolts, and the motor is fixedly connected to the middle of the bracket
through bolts. The power output end of the motor is fixedly connected with the rotating
rod by bolts. One end of the rotating rod passes through the base and the box body and is
fixedly connected with the gear by bolts. The gear meshes with the bottom ends of one
side of the two fixed cylinders. The inside of the fixed cylinders is filled with culture
medium. Both sides of the inner wall of the box body arefixedly connected with electric
heating wires by bolts, and both sides of the middle of the lower surface of the inner wall
of the box body are fixedly connected with support columns by bolts. The support
columns are slidably connected with the fixed cylinders. One side of the box body is
fixedly connected with a water inlet pipe by bolts, and the lower surface of the water inlet
pipe is fixedly connected with spray head by bolts at both ends near the two ends.
Preferably, the fixed cylinders further comprises openings, a culture medium and a
groove. The outer periphery of the fixed cylinders is provided with openings. A groove is
arranged in the middle of the lower surface. The support columns are slidably connected
with the groove.
Preferably, there are six openings in total with a diameter of 0.1-0.5 MM. The support
columns matches with the groove.
Preferably, one side of the box body is fixedly connected with a controller by bolts, and
both sides of the inner wall of the box body are fixedly connected with temperature
sensors by bolts. The controller input terminal is electrically connected with the
temperature sensors output terminal, and the controller output terminal is electrically
connected with the electric heating wire input terminal.
Preferably, the lower surface of the box body and the upper surface of the base are both
provided with through holes. There are two through holes and the through holes are
symmetrical with respect to the rotating rod. The lower surface of the box body has is arc
structure.
Preferably, the bracket is U-shaped. The spray head is located directly above the fixed
cylinder. The base is a hollow structure. One side of the base is fixedly connected with a
drain pipe by bolts, and one end of the drain pipe is in threaded connection with a sealing
plug.
The beneficial effects of the present invention are:
The invention is provided with fixed cylinders, a gear and a motor, and the motor drives
the rotating rod to rotate, thereby driving the gear to rotate, so as to drive two fixed
cylinders to rotate. On the one hand, by rotating the fixed cylinders, the culture medium
in the fixed cylinders can be heated uniformly, so that the conditions of microbial culture
will not change too much, ensuring that the cultivated microbes meet the requirements of
screening. On the other hand, during the rotation of the fixed cylinders, the sewage in the
fixed cylinders can be thrown out through the action of centrifugal force, and then the
sewage in the culture medium can be discharged and can be discharged from the box
body through the through holes and the drain pipe to avoid the contamination of the culture conditions during the microorganism culture process. In addition, through installation of the temperature sensor, controller and electric heating wire, the measured temperature is transmitted to the controller by temperate sensor, and the controller controls the temperature of the electric heating wire to increase or decrease, which can not only regulates the temperature in the box body, but also keep the temperature in the box body constant.
BRIEF DESCRIPTION OF THE FIGURES
Figure 1 is a schematic diagram of the three-dimensional structure of the present
invention;
Figure 2 is a schematic sectional view of the structure of the present invention;
Figure 3 is a schematic sectional view of the structure of the fixed cylinders of the present
invention;
Figure 4 is a schematic diagram of the electrical connection structure of the components
of the present invention.
In the figure: 100-sealing cover; 200-box body; 210-controller; 220-inlet pipe; 230-spray
head; 240-electric heating wire; 250-temperature sensor; 260-fixed cylinder; 261
opening; 262- culture medium; 263-groove; 270-gear; 280-support column; 290-through
hole; 300-base; 310-bracket; 320-motor; 330-rotating rod.
DESCRIPTION OF THE INVENTION
The following will clearly and completely describe the technical solutions in the
embodiments of the present invention with reference to the accompanying figures in the
embodiments of the present invention.
As shown in Figures 1-4, a microbial bed with temperature regulation includes a sealing
cover 100, a box body 200, and a base 300. The upper surface of the base 300 isfixedly
connected to the box body 200 by bolts. The box body 200 is plugged with the sealing
cover 100. The box body 200 is slidably connected with the sealing cover 100. The box
body 200 includes two fixed cylinders 260, a gear 270 and a water inlet pipe 220. A
bracket 310 is fixedly connected to the lower surface of the inner wall of the base 300
through bolts, and the motor 320 is fixedly connected to the middle of the bracket 310
through bolts. The power output end of the motor 320 is fixedly connected with a
rotating rod 330 through bolts. One end of the rotating rod 330 passes through the base
300 and the box body 200 and is fixedly connected with a gear 270 through bolts. The
gear 270 meshes with the bottom ends of one side of the two fixed cylinders 260. The
inside of the fixed cylinder 260 is filled with culture medium 262, and both sides of the
inner wall of the box body 200 arefixedly connected with electric heating wires 240 by
bolts. The model of the electric heating wire 240 is JHF-010. Both sides of the middle of
the lower surface of the inner wall of the box body 200 arefixedly connected with
support columns 280 by bolts. The support columns 280 are slidably connected with the
fixed cylinders 260. One side of the box body is fixedly connected with a water inlet pipe
220 by bolts, and the lower surface of the water inlet pipe is fixedly connected with spray
head 230 by bolts at both ends near the two ends. Thefixed cylinders further comprises
openings 261, a culture medium 262 and a groove 263. The outer periphery of the fixed
cylinders 260 is provided with openings 261. A groove 263 is arranged in the middle of
the lower surface. The support columns 280 are slidably connected with the groove 263.
There are six openings 261 in total with a diameter of 0.1-0.5 MM. The motor 320 drives the rotating rod 330 to rotate, thereby driving the gear 270 to rotate, so as to drive two fixed cylinders 260 to rotate. On the one hand, by rotating the fixed cylinders 260, the culture medium 262 in the fixed cylinders 260 can be heated uniformly, so that the conditions of microbial culture will not change too much, ensuring that the cultivated microbes meet the requirements of screening. On the other hand, during the rotation of the fixed cylinders 260 , the sewage in the fixed cylinders 260 can be thrown out through the action of centrifugal force, and then the sewage in the culture medium 262 can be discharged. The support columns 280 matches with the groove 263. One side of the box body 200 is fixedly connected with a controller 210 by bolts. The model of the controller
210 is PLCS7-200. Both sides of the inner wall of the box body 200 are fixedly
connected with temperature sensors 250 by bolts. The model of the temperature sensor
250 is DS18B20. The controller 210 input terminal is electrically connected with the
temperature sensors 250 output terminal, and the controller 210 output terminal is
electrically connected with the electric heating wire 240 input terminal. Through
installation of the temperature sensor 250, the measured temperature is transmitted to the
controller 210 by temperature sensor, and the controller 210 controls the temperature of
the electric heating wire 240 to increase or decrease, which can not only regulates the
temperature in the box body 200, but also keep the temperature in the box body 200
constant. The lower surface of the box body 200 and the upper surface of the base 300 are
both provided with through holes 290. There are two through holes 290 and the through
holes are symmetrical with respect to the rotating rod 330. The lower surface of the box
body 200 has is arc structure which is convenient to drain the sewage in the box 200
through the through holes 290. The bracket 310 is U-shaped which supports the motor
320. The spray head 230 is located directly above the fixed cylinders 260. The spray head
230 adds water to the microorganisms in the fixed cylinder 260. The base 300 is a hollow
structure. One side of the base 300 is fixedly connected with a drain pipe by bolts, and
one end of the drain pipe is in threaded connection with a sealing plug. When the sealing
plug is opened, the sewage in the base 300 can be drained.
Specific usage process: When in use, the culture medium 262 is placed in the fixed
cylinder 260, and the sealing cover 100 is plugged with the box body 200. The electric
heating wire 240, the motor 320, the controller 210 and the temperature sensor 250 are all
electrically connected to the power supply, so that the electric heating wire 240, the
motor 320, the controller 210 and the temperature sensor 250 are in a normal working
state, and the motor 320 drives the rotating rod 330 to rotate, which drives the gear 270 to
rotate, thereby driving two fixed cylinder 260 to rotate. On the one hand, by rotating the
fixed cylinders 260, the culture medium 262 in the fixed cylinders 260 can be heated
uniformly, so that the conditions of microbial culture will not change too much, ensuring
that the cultivated microbes meet the requirements of screening. On the other hand,
during the rotation of the fixed cylinders 260, the sewage in the fixed cylinders 260 can
be thrown out through the action of centrifugal force, and then the sewage in the culture
medium 262 can be discharged, Sewage enters the base 300 through the through holes
290, and can be discharged from the base 300 through a drain pipe to avoid
contamination of the culture conditions during the microorganism culture process In
addition, through installation of the temperature sensor 250, controller 210 and electric
heating wire 240, the measured temperature is transmitted to the controller 210 by
temperature sensor 250, and the controller 210 controls the temperature of the electric heating wire 240 to increase or decrease, which can not only regulates the temperature in the box body 200, but also keep the temperature in the box body 200 constant.
It should be noted that in this article, relational terms such as first and second are only
used to distinguish one entity or operation from another entity or operation, and It does
not necessarily require or imply any such actual relationship or order between these
entities or operations. Moreover, the terms "comprise", "include" or any other variants
thereof are intended to cover non-exclusive inclusion, so that a process, method, article or
device including a series of elements not only includes those elements, but also includes
other elements that are not explicitly listed, or also include elements inherent to this
process, method, article or equipment.
Although the embodiments of the present invention have been shown and described, for
those of ordinary skill in the field, various changes, modifications, substitutions, and
variations can be made to these embodiments without departing from the principle and
spirit of the present invention. The scope of the present invention is defined by the
appended claims and their equivalents.

Claims (6)

THE CLAIMS DEFINING THE INVENTION ARE AS FOLLOWS:
1. A microbial bed with temperature regulation, comprising a sealing cover, a box body
and a base, wherein the upper surface of the base is fixedly connected with the box body
by bolts; the box body is plugged with the sealing cover, and the box body is slidably
connected with the sealing cover; the box body includes two fixed cylinders, the gear and
a water inlet pipe; a bracket is fixedly connected to the lower surface of the inner wall of
the base through bolts, and the motor is fixedly connected to the middle of the bracket
through bolts; the power output end of the motor is fixedly connected with the rotating
rod by bolts; one end of the rotating rod passes through the base and the box body and is
fixedly connected with the gear by bolts; the gear meshes with the bottom ends of one
side of the two fixed cylinders; the inside of the fixed cylinders is filled with culture
medium; both sides of the inner wall of the box body are fixedly connected with electric
heating wires by bolts, and both sides of the middle of the lower surface of the inner wall
of the box body are fixedly connected with support columns by bolts; the support
columns are slidably connected with the fixed cylinders; one side of the box body is
fixedly connected with a water inlet pipe by bolts, and the lower surface of the water inlet
pipe is fixedly connected with spray head by bolts at both ends near the two ends.
2. The microbial bed with temperature regulation according to claim 1, characterized in
that: the fixed cylinders further comprises openings, a culture medium and a groove; the
outer periphery of the fixed cylinders is provided with openings; a groove is arranged in
the middle of the lower surface; the support columns are slidably connected with the
groove.
3. The microbial bed with temperature regulation according to claim 2, characterized in
that: there are six openings in total with a diameter of 0.1-0.5 MM and the support
columns matches with the groove.
4. The microbial bed with temperature regulation according to claim 1, characterized in
that: one side of the box body is fixedly connected with a controller by bolts, and both
sides of the inner wall of the box body are fixedly connected with temperature sensors by
bolts; the controller input terminal is electrically connected with the temperature sensors
output terminal, and the controller output terminal is electrically connected with the
electric heating wire input terminal.
5. The microbial bed with temperature regulation according to claim 1, characterized in
that: the lower surface of the box body and the upper surface of the base are both
provided with through holes; there are two through holes and the through holes are
symmetrical with respect to the rotating rod; the lower surface of the box body has is arc
structure.
6. The microbial bed with temperature regulation according to claim 1, characterized in
that: the bracket is U-shaped; the spray head is located directly above the fixed cylinder;
the base is a hollow structure; one side of the base is fixedly connected with a drain pipe
by bolts, and one end of the drain pipe is in threaded connection with a sealing plug.
AU2021104907A 2021-08-04 2021-08-04 Microbial Bed with Temperature Regulation Ceased AU2021104907A4 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2021104907A AU2021104907A4 (en) 2021-08-04 2021-08-04 Microbial Bed with Temperature Regulation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
AU2021104907A AU2021104907A4 (en) 2021-08-04 2021-08-04 Microbial Bed with Temperature Regulation

Publications (1)

Publication Number Publication Date
AU2021104907A4 true AU2021104907A4 (en) 2021-09-30

Family

ID=77857765

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2021104907A Ceased AU2021104907A4 (en) 2021-08-04 2021-08-04 Microbial Bed with Temperature Regulation

Country Status (1)

Country Link
AU (1) AU2021104907A4 (en)

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MK22 Patent ceased section 143a(d), or expired - non payment of renewal fee or expiry