AU2019397364B8 - Homing endonuclease variants - Google Patents

Homing endonuclease variants

Info

Publication number
AU2019397364B8
AU2019397364B8 AU2019397364A AU2019397364A AU2019397364B8 AU 2019397364 B8 AU2019397364 B8 AU 2019397364B8 AU 2019397364 A AU2019397364 A AU 2019397364A AU 2019397364 A AU2019397364 A AU 2019397364A AU 2019397364 B8 AU2019397364 B8 AU 2019397364B8
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AU
Australia
Prior art keywords
amino acid
group
amino
onui
aminoacid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2019397364A
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English (en)
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AU2019397364B1 (en
AU2019397364A1 (en
Inventor
Constantine CHRYSOSTOMOU
Kyle HAVENS
Jordan JARJOUR
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Publication of AU2019397364A1 publication Critical patent/AU2019397364A1/en
Assigned to 2SEVENTY BIO, INC. reassignment 2SEVENTY BIO, INC. Request for Assignment Assignors: BLUEBIRD BIO, INC.
Assigned to NOVO NORDISK A/S reassignment NOVO NORDISK A/S Request for Assignment Assignors: 2SEVENTY BIO, INC.
Publication of AU2019397364B1 publication Critical patent/AU2019397364B1/en
Application granted granted Critical
Publication of AU2019397364B8 publication Critical patent/AU2019397364B8/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2019397364A 2018-12-10 2019-12-09 Homing endonuclease variants Active AU2019397364B8 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862777476P 2018-12-10 2018-12-10
US62/777,476 2018-12-10
PCT/US2019/065211 WO2020123371A2 (en) 2018-12-10 2019-12-09 Homing endonuclease variants

Publications (3)

Publication Number Publication Date
AU2019397364A1 AU2019397364A1 (en) 2021-06-24
AU2019397364B1 AU2019397364B1 (en) 2025-07-17
AU2019397364B8 true AU2019397364B8 (en) 2025-08-07

Family

ID=71076132

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2019397364A Active AU2019397364B8 (en) 2018-12-10 2019-12-09 Homing endonuclease variants

Country Status (9)

Country Link
US (2) US12404500B2 (https=)
EP (1) EP3893922A4 (https=)
JP (2) JP2022513750A (https=)
KR (1) KR20210102925A (https=)
CN (1) CN113329760A (https=)
AU (1) AU2019397364B8 (https=)
CA (1) CA3122278A1 (https=)
IL (1) IL283720B2 (https=)
WO (1) WO2020123371A2 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2024503249A (ja) * 2020-12-21 2024-01-25 ２セブンティ バイオ インコーポレイテッド 部位特異的変異導入のための組成物及び方法

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WO2018022619A1 (en) * 2016-07-25 2018-02-01 Bluebird Bio, Inc. Bcl11a homing endonuclease variants, compositions, and methods of use
WO2018035141A1 (en) * 2016-08-16 2018-02-22 Bluebird Bio, Inc. Il-10 receptor alpha homing endonuclease variants, compositions, and methods of use
WO2018049226A1 (en) * 2016-09-08 2018-03-15 Bluebird Bio, Inc. Pd-1 homing endonuclease variants, compositions, and methods of use
WO2018075541A1 (en) * 2016-10-17 2018-04-26 Bluebird Bio, Inc. TGFβR2 ENDONUCLEASE VARIANTS, COMPOSITIONS, AND METHODS OF USE

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US5804413A (en) 1992-07-31 1998-09-08 University Of Pittsburgh Of The Commonwealth System Of Higher Education Herpes simplex virus strains for gene transfer
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ATE318923T1 (de) 2000-06-01 2006-03-15 Univ North Carolina Doppelsträngige parvovirus-vektoren
FR2872170B1 (fr) 2004-06-25 2006-11-10 Centre Nat Rech Scient Cnrse Lentivirus non interactif et non replicatif, preparation et utilisations
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EP2524037B1 (en) 2010-01-12 2018-05-16 The University Of North Carolina At Chapel Hill Restrictive inverted terminal repeats for viral vectors
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US20140148361A1 (en) 2010-06-07 2014-05-29 Barry L. Stoddard Generation and Expression of Engineered I-ONUI Endonuclease and Its Homologues and Uses Thereof
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Publication number Priority date Publication date Assignee Title
US20110256607A1 (en) * 2010-04-16 2011-10-20 Georg Hausner Homing endonucleases
WO2018022619A1 (en) * 2016-07-25 2018-02-01 Bluebird Bio, Inc. Bcl11a homing endonuclease variants, compositions, and methods of use
WO2018035141A1 (en) * 2016-08-16 2018-02-22 Bluebird Bio, Inc. Il-10 receptor alpha homing endonuclease variants, compositions, and methods of use
WO2018049226A1 (en) * 2016-09-08 2018-03-15 Bluebird Bio, Inc. Pd-1 homing endonuclease variants, compositions, and methods of use
WO2018075541A1 (en) * 2016-10-17 2018-04-26 Bluebird Bio, Inc. TGFβR2 ENDONUCLEASE VARIANTS, COMPOSITIONS, AND METHODS OF USE

Also Published As

Publication number Publication date
IL283720B1 (en) 2025-01-01
US20250368973A1 (en) 2025-12-04
CN113329760A (zh) 2021-08-31
JP2025072470A (ja) 2025-05-09
JP2022513750A (ja) 2022-02-09
AU2019397364B1 (en) 2025-07-17
WO2020123371A2 (en) 2020-06-18
IL283720B2 (en) 2025-05-01
EP3893922A2 (en) 2021-10-20
US12404500B2 (en) 2025-09-02
KR20210102925A (ko) 2021-08-20
US20220119784A1 (en) 2022-04-21
EP3893922A4 (en) 2022-09-14
WO2020123371A3 (en) 2020-08-20
AU2019397364A1 (en) 2021-06-24
CA3122278A1 (en) 2020-06-18
IL283720A (en) 2021-07-29

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Legal Events

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PC1 Assignment before grant (sect. 113)

Owner name: 2SEVENTY BIO, INC.

Free format text: FORMER APPLICANT(S): BLUEBIRD BIO, INC.

PC1 Assignment before grant (sect. 113)

Owner name: NOVO NORDISK A/S

Free format text: FORMER APPLICANT(S): 2SEVENTY BIO, INC.

TH Corrigenda

Free format text: IN VOL 59 , NO 29 , PAGE(S) 4093 UNDER THE HEADING APPLICATIONS ACCEPTED - NAME INDEX UNDER THE NAME NOVO NORDISK A/S, APPLICATION NO. 2019397364, UNDER INID (43) CORRECT THE PUBLICATION DATE TO READ 18.06.2020

FGA Letters patent sealed or granted (standard patent)