AU2019100730A4 - Use of Composition of Sunflower Head, Gorgon Euryale Seed and Kudzuvine Root in Lowering Uric Acid and Repair of Liver and Kidney Injury - Google Patents

Abstract

Abstract The present invention provides a compound composition of sunflower head, gorgon euryale seed and kudzuvine root, wherein raw materials, i.e., sunflower head, gorgon euryale seed and kudzuvine root, are used to make up a compound preparation. The synergistic action of the three components not only strengthens kidney repair ability, but also promotes the discharge of metabolites through the kidney, thereby providing an overall comprehensive solution to the side effects of the above-mentioned drugs. Through animal experiments and clinical medical treatments, it is proved that the composition of sunflower head, gorgon euryale seed and kudzuvine root can repair liver and kidney injury, and can be used continuously in a long-term medication. Fig I ,.a~ *cjpY 'felt.' livrtssekinetisu Fig.1 7:-: or 7'; W-L ""4Vim At A A liver tissue kidney tissue Fig.2

Description

Use of Composition of Sunflower Head, Gorgon Euryale Seed and Kudzuvine Root in Lowering Uric Acid and Repair of Liver and Kidney Injury

Technical Field [0001] The present invention discloses a composition of sunflower head, gorgon euryale seed and kudzuvine root, wherein sunflower head, gorgon euryale seed and kudzuvine root are used to make up a compound preparation. The compound preparation has an obvious repair effect on liver and kidney injury and belongs to the field of traditional Chinese medicine pharmaceutical technology.

Background Art [0002] Gout is an arthropathy caused by a purine metabolism disorder and reduced uric acid excretion as well as monosodium urate deposition. In China, the prevalence rate of hyperuricemia is 5.0% - 23.4%, while the prevalence rate of gout is 1% - 3%. Urate can deposit in any site of the body except the nerve centre due to the protection of blood-brain barrier. Urate crystallized and deposited on joints may damage the joints. These diseases result from oxidative stress and inflammatory reactions caused by uric acid and urate crystal deposited in the kidney. A sharp rise of uric acid level in a short time can lead to acute uric acid nephropathy (UAN), while elevated uric acid level for a long term may cause chronic urate nephropathy and uric acid nephrolithiasis, which impair renal function and at the end stage may lead to uremia. Primary hyperuricemia is often accompanied with three highs - high blood pressure, high blood lipid and diabetes. In view of the situation that uric acid-lowering drugs have certain toxicity on liver and kidney, the present invention has a certain effect in lowering uric acid and repairing liver and kidney injury.

[0003] The sunflower small molecule peptide extracted from sunflower head has the functions of inhibiting inflammation, reducing swelling, relieving pains, lowering blood uric acid, and dissolving sodium urate, and has a good repair effect on damaged kidney cells; therefore, it can address both symptoms and root causes of hyperuricemia, and treat hyperuricemia radically. Kudzu root contains 12% flavonoids, such as

2019100730 03 Jul 2019 puerarin, soy flavonoid glycosides, and peanut agglutinin, and thus it can protect the liver and effectively improve the regeneration ability of liver cells, and have the function of lowering the “three highs”. Gorgon euryale seed is sweet, puckery and natured and has the effect of tonifying spleen and reinforcing kidney. The present invention can be used as both food and medicine, combines sunflower head, gorgon euryale seed and kudzuvine root to provide a natural compound composition for treating liver and kidney injury with no toxic or side effects. The compound composition can effectively treat gout by lowering uric acid and repair liver and kidney injury caused by hyperuricemia.

Summary of the Invention [0004] The present invention provides a compound composition of sunflower head, gorgon euryale seed and kudzuvine root, wherein raw materials, i.e., sunflower head, gorgon euryale seed and kudzuvine root, are used to make up a compound preparation for lowering uric acid to treat gout and repairing liver and kidney injury. The compound composition solves the problem that currently available drugs for treating gout and liver and kidney injury have side effects, and can be used in a long-term medication for nursing the problems of kidney-yin deficiency and kidney-yang deficiency. The present invention proves that gorgon euryale seed and kudzuvine root can help to enhance the ability of the sunflower head to repair liver and kidney cells, promote the repair of liver and kidney tissues, and strengthen the recovery of liver and kidney functions, and the three components have a good synergistic effect.

[0005] The present invention provides a compound composition of sunflower head, gorgon euryale seed and kudzuvine root, wherein the compound composition is made of the following raw materials in parts by mass:

sunflower head: 50-70 parts; gorgon euryale seed: 15-25 parts; and kudzuvine root: 15-25 parts.

[0006] The present invention relates to a compound composition of sunflower head, gorgon euryale seed and kudzuvine root, preferably in the following ratio:

sunflower head: 60 parts; gorgon euryale seed: 20 parts; and kudzuvine root: 20

2019100730 03 Jul 2019 parts.

[0007] The present invention relates to a method for preparing a compound composition of sunflower head, gorgon euryale seed and kudzuvine root, comprising the following steps:

step 1), taking 50-70 parts of sunflower head, and adding 10-20-fold by mass of water; after soaking sufficiently, adding neutral protease with a mass fraction of 0.2% to perform enzymolysis under a pH of 6.0-7.0 at a temperature of 40-50°C for 1-2 hours; then adjusting the pH to 8; and performing full reaction in a reactor at 40-50°C for 40-60 min to obtain a sunflower head extract;

step 2), taking 10-30 parts of gorgon euryale seed, and adding 10-20-fold by mass of 70% ethanol; sufficiently soaking for 1-2 h; and performing reflux extraction in an extracting tank for 1-3 h to recover ethanol to obtain a gorgon euryale seed extract;

step 3), taking 10-30 parts of kudzuvine root, and adding 10-20-fold by mass of water; sufficiently soaking for 1 -2 h; and performing reflux extraction in an extracting tank for 1 -3 h to obtain a kudzuvine root extract; and step 4), combining the extracts obtained in steps 1), 2), and 3); concentrating to a clear paste with a relative density of 1.10 - 1.30; drying; and grinding into a fine powder.

[0008] The basis of the formulating prescription of the present invention is as follows:

the formulating prescription described in the present invention is based on the recordings about traditional Chinese medicines for treating gout and repairing liver and kidney injury and the modem Chinese medicine theory and is summarized by a large number of animal experiments and clinical cases.

[0009] The sources and efficacy of raw materials used in the present invention are as follows:

1. Sunflower head, a sweet medicinal material that is non-toxic and cold in property, targets the kidney meridian; and according to “Chinese Materia Medica”, it has the functions of clearing away heat, freeing lung, relieving pain, and stopping bleeding, and is mainly used for headache and dizziness treatment, anti-inflammation,

2019100730 03 Jul 2019 diuresis, kidney protection and the like.

[0010] 2. Gorgon euryale seed, a sweet, puckery and natured medicinal material, has the functions of invigorating spleen to arrest diarrhea, tonifying kidney to secure essence, removing dampness and arresting leukorrhea. It is used for the treatment of symptoms such as nocturnal emission and spermatorrhea, enuresis and frequent urination, chronic diarrhea because of spleen deficiency, and turbid leucorrhea.

[0011] 3. Kudzu root, a sweet, spicy and cool medicinal material, targets the spleen, stomach, and lung meridians. It can relieve muscle pain, bring down a fever, promote eruption, help produce saliva and slake thirst, enhance yang-qi and stop diarrhea, clear and activate channels and collaterals, and relieve alcoholism. It is used for the treatment of exogenous fever and headache, acute neck and back pain, thirst, consumptive thirst, measles without adequate eruption, diarrhea with fever, diarrhea, dizziness and headache, apoplexy hemiplegia, thoracic obstruction and cardiodynia, and alcoholic poisoning and visceral injury.

[0012] The positive effects and advantages of the present invention are as follows:

Common medicines for treating gout and hyperuricemia are non-steroidal anti-inflammatory drugs, alkaloids, glucocorticoids, etc., whose roles are mainly inhibiting uric acid production and promoting uric acid excretion. All the above-mentioned drugs can cause different degrees of damage to liver and kidney. The present invention has dual effects of inhibiting uric acid production and promoting uric acid excretion, wherein sunflower head, gorgon euryale seed and kudzuvine root are used to make up a compound preparation, the synergistic action of the three components not only strengthens kidney repair ability, but also promotes the discharge of metabolites through the kidney, thereby providing an overall comprehensive solution to the side effects of the above-mentioned drugs. Through animal experiments and clinical medical treatments, it is proved that the composition of sunflower head, gorgon euryale seed and kudzuvine root can lower uric acid, treat gout, and repair liver and kidney injury, and can be used continuously in a long-term medication.

Brief Description of the Drawings

2019100730 03 Jul 2019 [0013] Figure 1 shows liver and kidney tissue sections of the blank control group;

Figure 2 shows liver and kidney tissue sections of the model group;

Figure 3 shows liver and kidney tissue sections of the positive control group;

Figure 4 shows liver and kidney tissue sections of the gorgon euryale seed extract group;

Figure 5 shows liver and kidney tissue sections of the sunflower head extract group;

Figure 6 shows liver and kidney tissue sections of the kudzuvine root extract group;

Figure 7 shows liver and kidney tissue sections of the sunflower head, gorgon euryale seed and kudzuvine root composition group 1 of the present invention;

Figure 8 shows liver and kidney tissue sections of the sunflower head, gorgon euryale seed and kudzuvine root composition group 2 of the present invention;

Figure 9 shows liver and kidney tissue sections of the sunflower head, gorgon euryale seed and kudzuvine root composition group 3 of the present invention.

Detailed Description of Embodiments [0014] In order to make a person skilled in the art better understand and implement the technical solutions of the present invention, the present invention will be further described below in conjunction with specific experimental examples. However, the experimental examples are not intended to limit the present invention.

[0015] Example 1

Step 1) comprises: taking 50 parts of sunflower head, and adding 10-fold by mass of water; after soaking sufficiently, adding neutral protease with a mass fraction of 0.2% to perform enzymolysis under a pH of 6.0 at a temperature of 40°C for 1 hours, and then adjusting the pH to 8; and performing full reaction in a reactor at 40°C for 40 min to obtain a sunflower head extract;

step 2) comprises: taking 25 parts of gorgon euryale seed, and adding 10-fold by mass of 70% ethanol; sufficiently soaking for 1 h; and performing reflux extraction in an extracting tank for 1 h to recover ethanol to obtain a gorgon euryale seed extract;

2019100730 03 Jul 2019 step 3) comprises: taking 25 parts of kudzuvine root, and adding 10-fold by mass of water; sufficiently soaking for 1 h; and performing reflux extraction in an extracting tank for 1 h to obtain a kudzuvine root extract; and step 4) comprises: combining the extracts obtained in steps 1), 2), and 3); concentrating to a clear paste with a relative density of 1.10; drying; and grinding into a fine powder.

[0016] Example 2

Step 1) comprises: taking 70 parts of sunflower head, and adding 15-fold by mass of water; after soaking sufficiently, adding neutral protease with a mass fraction of 0.2% to perform enzymolysis under a pH of 6.5 at a temperature of 45°C for 1.5 hours, and then adjusting the pH to 8; and performing full reaction in a reactor at 45°C for 45 min to obtain a sunflower head extract;

step 2) comprises: taking 15 parts of gorgon euryale seed, and adding 15-fold by mass of 70% ethanol; sufficiently soaking for 1.5 h; and performing reflux extraction in an extracting tank for 2 h to recover ethanol to obtain a gorgon euryale seed extract;

step 3) comprises: taking 15 parts of kudzuvine root, and adding 15-fold by mass of water; sufficiently soaking for 1.5 h; and performing reflux extraction in an extracting tank for 2 h to obtain a kudzuvine root extract; and step 4) comprises: combining the extracts obtained in steps 1), 2), and 3); concentrating to a clear paste with a relative density of 1.20; drying; and grinding into a fine powder.

[0017] Example 3 step 1) comprises: taking 60 parts of sunflower head, and adding 20-fold by mass of water; after soaking sufficiently, adding neutral protease with a mass fraction of 0.2% to perform enzymolysis under a pH of 7.0 at a temperature of 50°C for 2 hours, and then adjusting the pH to 8; and performing full reaction in a reactor at 50°C for 50 min to obtain a sunflower head extract;

step 2) comprises: taking 20 parts of gorgon euryale seed, and adding 20-fold by mass of 70% ethanol; sufficiently soaking for 2 h; and performing reflux extraction in an extracting tank for 3 h to recover ethanol to obtain a gorgon euryale seed extract;

2019100730 03 Jul 2019 step 3) comprises: taking 20 parts of kudzuvine root, and adding 20-fold by mass of water; sufficiently soaking for 2 h; and performing reflux extraction in an extracting tank for 3 h to obtain a kudzuvine root extract; and step 4) comprises: combining the extracts obtained in steps 1), 2), and 3); concentrating to a clear paste with a relative density of 1.20; drying; and grinding into a fine powder.

[0018] I. An animal experiment was used below to verify the repair effect of the present invention on injuries of liver and kidney tissues and damage of liver and kidney functions:

(I) Repair of kidney injury caused by hyperuricemia

1. Experiment animals

Kunming male mice of SPF grade, with a body mass of 20 ±2g, purchased from Liaoning Changsheng Biotechnology Co., Ltd.

[0019] 2. Experiment reagents

Yeast extract (Oxiod Company, UK);

Xiuzheng Shenbao Mixture (positive control drug): Tongyao Pharmaceutical Group Co., Ltd., lot number: 151102;

gorgon euryale seed extract;

sunflower head extract;

kudzu root extract;

the composition of sunflower head, gorgon euryale seed and kudzuvine root obtained in Example 1;

the composition of sunflower head, gorgon euryale seed and kudzuvine root obtained in Example 2;

the composition of sunflower head, gorgon euryale seed and kudzuvine root obtained in Example 3;

3. Modelling and administration method

The mice were weighed before the study, 10 of which were used as the blank control group, and the remaining mice were used as the hyperuricemia model group. The mice were weighed every morning during the experiment. The model was

2019100730 03 Jul 2019 established by intragastric administration of yeast extract, which increased UA value and disturbed purine metabolism by administration of a large amount of purine substances, so that the blood UA was temporarily increased in a short time. 80 mice were randomly divided into 8 groups (10 mice per group), i.e., blank control group, model group, positive control (Shenbao) group, gorgon euryale seed extract group, sunflower head extract group, kudzuvine root group, Example 1 group, Example 2 group, and Example 3 group, respectively. During the process of modelling, except for the blank control group (intragastric administration of an equal volume of water), mice in each of other groups received intragastric administration of 20 g’kg ' yeast extract every day, once a day, and the intragastric administration was continued for 20 d. Since day 9, intragastric administration of 200 μΐ/d drug was performed at the same time until the end of the experiment. Kidney tissues were taken from the mice, made into liver and kidney tissue sections, and stained with HE. The changes of the tissue sections were observed under a microscope. (Figures 1-8)

The experiment results were shown in Figures 1-8. In the blank group (Figure 1), hepatocytes and hepatocyte cords in the hepatic lobules were clear, and hepatocyte cytoplasm was full; the cytoplasm of renal tubular epithelial cells was not full but was basically normal. In the model group (Figure 2), hepatocytes and hepatocyte cords in partial hepatic lobules were not clear, and hepatocyte atrophy was seen with punctate necrosis lesions and eosinophilic changes of hepatocytes; and eosinophilic changes of renal tubular epithelial cells were obvious, and cavitation, breakage, and karyopyknosis of renal tubular epithelial cells were observed. This indicates that the kidney injury model has been successfully established. In the positive control group (Figure 3), the hepatocytes and hepatocyte cords in the hepatic lobules were clear, the hepatocyte cytoplasm was full, and punctate necrosis lesions were occasionally seen. There were slight cavitation of renal tubular epithelial cells and occasional eosinophilic changes of renal tubular epithelial cells. In the gorgon euryale seed extract group (Figure 4), punctate necrosis lesions and eosinophilic changes were occasionally seen, and renal tubular epithelial cytoplasm was not full but was basically normal. This indicates that the gorgon euryale seed extract has a good repair effect on

2019100730 03 Jul 2019 liver and kidney tissues. In the sunflower extract group and the kudzuvine root extract group (Figures 5 and 6), the hepatocytes and hepatocyte cords in the hepatic lobules were clear, the hepatocyte cytoplasm was full, and punctate necrosis lesions were occasionally seen; and eosinophilic changes of renal tubular epithelial cells were obvious, and cavitation and breakage of renal tubular epithelial cells were observed. In Example 1 group, Example 2 group, and Example 3 group (Figures 7-9), the hepatocytes and hepatocyte cords in hepatic lobules were clear, and punctate necrosis lesions were occasionally seen; and renal tubular epithelial cytoplasm was not full, and eosinophilic changes and epithelial cell cavitation were occasionally seen. Moreover, the eosinophilic changes and cavitation in the Example 3 group were alleviated more significantly. The group with the best effect is the Example 3 group.

[0020] (II) Repair of kidney-yin deficiency caused by hydrocortisone

1. Experiment animals

KM mice of SPF grade: male, with a body mass of 20 ±2g, supplied by the Animal Experimental Centre of the First Hospital of Jilin University.

[0021 ] 2. Experiment reagents

Hydrocortisone: MYM BIOLOGICAL TECHNOLOGY COMPANY, lot number: 20160812.

[0022] Xiuzheng Shenbao Mixture (positive control drug): Tongyao Pharmaceutical Group Co., Ltd., lot number: 151102.

[0023] gorgon euryale seed extract;

sunflower head extract;

kudzu root extract;

the composition of sunflower head, gorgon euryale seed and kudzuvine root obtained in Example 1;

the composition of sunflower head, gorgon euryale seed and kudzuvine root obtained in Example 2;

the composition of sunflower head, gorgon euryale seed and kudzuvine root obtained in Example 3;

mouse corticosterone (Cor.), thyroxine (T4), testosterone (T) Elisa assay kit:

2019100730 03 Jul 2019

Nanjing Jiancheng Bioengineering Institute.

[0024] 3. Modelling and administration

The mice were weighed before the experiment. After three days of normal feeding, the modelling experiment was carried out, and the mice were weighed once every four days and recorded.

[0025] The mice were divided equally into 8 groups (10 mice per group), which were labelled as blank group, model group, positive control (Shenbao) group, gorgon euryale seed extract group, sunflower head extract group, kudzuvine root extract group, Example 1 group, Example 2 group, and Example 3 group, respectively. The blank group received intragastric administration of 0.2 mL/d distilled water and had free access to water and food for 22 days; the model group received intragastric administration of 0.2 mL/d distilled water and had free access to water and food for 14 days, and since day 15, received intragastric administration of 50 mg/kg hydrocortisone for one week; and all the remaining groups received intragastric administration of 0.2 mL/d continuously for 22 days, and since day 15, received intragastric administration of 50 mg/kg hydrocortisone at the same time for one week. The mice were fasted on the evening of day 22 and blood was taken on the next day to test the contents of thyroxine (T4), testosterone (T), and corticosterone (Cor.) in the serum.

[0026] 4. Experiment results

Table 1. Detection of biochemical indicators in each experimental group:

	Thyroxine (ng/ml)	Testosterone (nmol/L)	Corticosterone (ng/ml)
Blank group	129.76 ± 15.10	4.67 ±0.66	257.91 ±27.66
Model group	98.74 ± 19.91	10.03 ±0.79	173.51 ±30.04
Shenbao group	107.63 ± 10.77**	8.05 ±0.92	202.19 ± 19.36**
Gorgon euryale seed extract	113.03 ±22.12**	8.66 ± 1.29	210.17 ±35.92**
Sunflower head extract	114.93 ± 10.69**	7.16 ± 1.18	210.49 ±39.15**
Kudzu root extract	115.81 ± 10.69**	7.33 ± 1.62	210.89 ±39.42**

2019100730 03 Jul 2019

Example 1	117.63 ±24.51**	6.34 ±0.27	217.65 ±29.79**
Example 2	119.35 ±27.67**	6.13 ± 1.08	239.17 ±44.70**
Example 3	125.73 ± 16.67**	5.02 ±0.98	248.63 ± 30.27**

Note: compared with the model group, *P < 0.05, **P < 0.01.

[0027] As can be seen from Table 1, compared with the blank control group, in the model group, the thyroxine and corticosterone contents were both decreased significantly while the testosterone content was increased, indicating that the model of kidney-yang deficiency was successfully established. Compared with the model group, in the example groups of the invention, the serum testosterone level was decreased while the thyroxine and corticosterone levels were both increased significantly (*P < 0.05, **P < 0.01), in which the increasing trend of the Example 3 group was more obvious, indicating that the combined action of sunflower head, gorgon euryale seed and kudzuvine root has a synergistic effect and has a better repair effect on kidney. [0028] (III) Repair of kidney-yang deficiency caused by hydrocortisone in mice

1. Experiment animals

KM mice of SPF grade: male, with a body mass of 20 ±2g, supplied by the Animal Experimental Centre of the First Hospital of Jilin University.

[0029] 2. Experiment reagents

Hydrocortisone: MYM BIOLOGICAL TECHNOLOGY COMPANY, lot number: 20160812.

[0030] Xiuzheng Shenbao Mixture (positive control drug): Tongyao Pharmaceutical Group Co., Ltd., lot number: 151102.

[0031 ] gorgon euryale seed extract;

sunflower head extract;

kudzu root extract;

the composition of sunflower head, gorgon euryale seed and kudzuvine root obtained in Example 1 the composition of sunflower head, gorgon euryale seed and kudzuvine root obtained in Example 2;

the composition of sunflower head, gorgon euryale seed and kudzuvine root

2019100730 03 Jul 2019 obtained in Example 3;

Mouse corticosterone (Cor.), thyroxine (T4), testosterone (T) Elisa assay kit: Nanjing Jiancheng Bioengineering Institute.

[0032] 3. Modelling and administration

The mice were weighed before the experiment. After three days of normal feeding, the modelling experiment was carried out, and the mice were weighed once every four days and recorded. The mice were divided equally into 8 groups (10 mice per group), which were labelled as blank group, model group, positive control (Shenbao) group, gorgon euryale seed extract group, sunflower head extract group, Example 1 group, Example 2 group, and Example 3 group, respectively. The blank group received intragastric administration of 0.2 mL/d distilled water and had free access to water and food; the model group received intragastric administration of 0.2 mL/d distilled water and had free access to water and food for 14 days, and since day 15, received intragastric administration of 25 mg/kg hydrocortisone for 10 days; and all the remaining groups received intragastric administration of 0.2 mL/d continuously for 25 days, and since day 15, received intragastric administration of 25 mg/kg hydrocortisone at the same time. The mice were fasted on the evening of day 25 and blood was taken on the next day to test the contents of thyroxine (T4), testosterone (T), and corticosterone (Cor.) in the serum.

[0033] 4. Experiment results

Table 2. Detection of biochemical indicators in each experimental group:

	Thyroxine (ng/ml)	Testosterone (nmol/L)	Corticosterone (ng/ml)
Blank group	56.54 ±9.79	4.03 ± 0.93	160.93 ±37.91
Model group	112.34 ± 19.95	3.74 ±0.82	249.74 ±31.23
Shenbao group	63.65 ±7.63**	5.13 ±0.33	162.81 ±24.98**
Gorgon euryale seed extract	67.73 ± 10.51**	5.92 ± 1.24	173.62 ±33.28**
sunflower head extract	63.18 ± 11.97**	5.87±0.16	170.39 ±45.66**
Kudzu root extract	62.65 ± 11.24**	5.63 ±0.18	170.12 ±45.42**
Example 1	61.91 ± 13.49**	5.19 ± 0.91	168.88 ±22.43**

2019100730 03 Jul 2019

Example 2	60.14 ±9.22**	4.78 ±0.88	168.94 ±34.19**
Example 3	58.39 ±6.75**	4.62 ±0.31	165.15 ±30.08**

Note: compared with the model group, *P < 0.05, **P < 0.01.

[0034] As can be seen from Table 1, compared with the blank control group, in the model group, the testosterone content was decreased while the thyroxine and corticosterone contents were both increased significantly, indicating that the model of kidney-yin deficiency was successfully established. Compared with the model group, in the example groups, the serum testosterone level was increased while the thyroxine and corticosterone levels were both decreased significantly (*P < 0.05, **P < 0.01), and the decreasing trend of the Example 3 group was more obvious, indicating that the combined action of sunflower head, gorgon euryale seed and kudzuvine root has a synergistic effect and a better repair effect on kidney.

[0035] Conclusion: the present invention provides the application of the compound extract of sunflower head, gorgon euryale seed and kudzuvine root in the treatment of gout by lowering uric acid and in the repair of liver and kidney injury, and it is proved through experiment that the compound extract has a good repair effect; moreover, the combined use of sunflower head, gorgon euryale seed and kudzuvine root has a more obvious effect than the use of one of them alone; and the three components have a synergistic effect and can significantly enhance their ability to repair damaged liver and kidney. Moreover, sunflower head, gorgon euryale seed and kudzuvine root are medicinal and edible natural materials with no toxic or side effects, and the synergistic action of the three components can strengthen the repair of liver and kidney tissues and functions, and provide an effective method and theoretical basis for long-term medication.

[0036] II. The following typical cases further demonstrate the efficacy of the present invention

Case 1

Patient Zhang, male, 60 years old, had gout for many years with repeated relapses, with blood uric acid value of 700 gmol/L and serum creatinine of 127 gmol/L. After taking a single sunflower head drug for three weeks, blood uric acid value was

2019100730 03 Jul 2019 decreased and was stabilized at 400 μιηοΙ/L, but the patient experienced dizziness, dysphoria and lack of sleep, and occasional fever. It was considered that the patient had gout for many years, his serum creatinine level was high, and uric acid had been accumulated and metabolised in the blood for a long time, which caused a serious burden on the kidney and impaired the kidney function. After a change to taking the compound composition of the present invention for one month, his serum uric acid level was stabilized at 370 μιηοΙ/L, and his serum creatinine was lowered to 90 μιηοΙ/L; and one month after termination of medication, both of the blood uric acid level and serum creatinine level were tested to be stable and no relapse occurred.

[0037] Case 2

Patient Wang, male, 37 years old, was found with elevated blood uric acid value during the physical examination last year. In the hospital, his blood uric acid level was tested to be 490 pmol/L and his serum creatinine level was 100 μιηοΙ/L. After taking a single sunflower head drug for two weeks, his blood uric acid level was dropped to 340 μιηοΙ/L, and his serum creatinine level was 102 μιηοΙ/L. The patient frequently experienced lack of energy, fatigue, soreness and weakness of waist and knees, and edema in the morning. However, since the patient had irregular diet and work and rest hours, it is considered that the patient had kidney deficiency. After taking the compound composition of the present invention for half a month, his blood uric acid level became stable at 340 μιηοΙ/L and serum creatinine level was stabilized at 90 μιηοΙ/L, indicating that his serum creatinine level was decreased. The patient's fatigue was relieved, and no relapse occurred half a year after termination of medication. [0038] Case 3

Patient Li, male, 51 years old, had hyperuricemia for more than ten years, and was complicated with symptoms of gout last year, with blood uric acid value of 516 μιηοΙ/L and serum creatinine of 130 μιηοΙ/L. After taking a single sunflower head drug for half a month, the patient’s blood uric acid value was decreased to 390 μιηοΙ/L and serum creatinine was 128 μιηοΙ/L, and was complicated with the symptoms of soreness and weakness of waist and knees, dizziness and tinnitus for a long time. It is considered that the patient had high blood uric acid level for a long time over the past

2019100730 03 Jul 2019 ten years, which caused damage to the kidney. After a change to taking the compound composition of the present invention for one month, his blood uric acid value was stablized at 380 pmol/L and serum creatinine level was stabilized at 87 μιηοΙ/L, and the symptoms of soreness and weakness of waist and knees, dizziness and tinnitus disappeared, indicating that the kidney function was well repaired. Three months after termination of medication, no relapse was reported at follow-up.

[0039] The detailed description set forth in series is intended to illustrate the feasible implementations of the present invention, and is not intended to limit the scope of protection of the invention. Any equivalent implementations or changes made without departing from the spirit of the present invention shall be included in the scope of protection of the present invention.

Claims (3)

Claims

1. A compound composition of sunflower head, gorgon euryale seed and kudzuvine root, wherein the compound composition is made of the following raw materials in parts by mass:

sunflower head: 50-70 parts; gorgon euryale seed: 15-25 parts; and kudzuvine root: 15-25 parts.

2. The compound composition of sunflower head, gorgon euryale seed and kudzuvine root according to claim 1, preferably in the following ratio:

sunflower head: 60 parts; gorgon euryale seed: 20 parts; and kudzuvine root: 20 parts.

3. A method for preparing the compound composition of sunflower head, gorgon euryale seed and kudzuvine root according to claim 1 or claim 2, comprising the following steps:

step 1), taking 50-70 parts of sunflower head, and adding 10-20-fold by mass of water; after soaking sufficiently, adding neutral protease with a mass fraction of 0.2% to perform enzymolysis under a pH of 6.0-7.0 at a temperature of 40-50°C for 1-2 hours; then adjusting the pH to 8; and performing full reaction in a reactor at 40-50°C for 40-60 min to obtain a sunflower head extract;

step 2), taking 10-30 parts of gorgon euryale seed, and adding 10-20-fold by mass of 70% ethanol; sufficiently soaking for 1-2 h; and performing reflux extraction in an extracting tank for 1-3 h to recover ethanol to obtain a gorgon euryale seed extract;

step 3), taking 10-30 parts of kudzuvine root, and adding 10-20-fold by mass of water; sufficiently soaking for 1 -2 h; and performing reflux extraction in an extracting tank for 1 -3 h to obtain a kudzuvine root extract; and step 4), combining the extracts obtained in steps 1), 2), and 3); concentrating to a clear paste with a relative density of 1.10 - 1.30; drying; and grinding into a fine powder.