CN109260266A - The purposes of sunflower disk Gorgon fruit and pueraria lobata compound in anti-trioxypurine reparation lesions of liver and kidney - Google Patents
The purposes of sunflower disk Gorgon fruit and pueraria lobata compound in anti-trioxypurine reparation lesions of liver and kidney Download PDFInfo
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- CN109260266A CN109260266A CN201811509838.8A CN201811509838A CN109260266A CN 109260266 A CN109260266 A CN 109260266A CN 201811509838 A CN201811509838 A CN 201811509838A CN 109260266 A CN109260266 A CN 109260266A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/488—Pueraria (kudzu)
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- A—HUMAN NECESSITIES
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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- A61P13/12—Drugs for disorders of the urinary system of the kidneys
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- A61P19/00—Drugs for skeletal disorders
- A61P19/06—Antigout agents, e.g. antihyperuricemic or uricosuric agents
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Abstract
The present invention provides a kind of sunflower disk Gorgon fruit and Gegen Compound compositions, it is that compound formulation is made using sunflower disk, Gorgon fruit, pueraria lobata as raw material, utilize the synergistic effect of three, while strengthening kidney repair ability, discharge of the metabolin through kidney is promoted, it is whole comprehensively to solve the side effect for taking said medicine.It is proved through zoopery and clinical treatment, sunflower disk, Gorgon fruit and pueraria lobata compound have the function of repairing lesions of liver and kidney, and can adhere to taking for a long time.
Description
Technical field
The present invention discloses a kind of sunflower disk Gorgon fruit and pueraria lobata compound, forms compound preparation by sunflower disk, Gorgon fruit and pueraria lobata,
There is apparent repairing effect for lesions of liver and kidney, belong to Chinese medicine pharmaceutical technology field.
Background technique
Purine metabolic disturbance and the joint caused disease of underexcretion and monosodium urate mineralization are both gout.In China,
The illness rate of hyperuricemia is 5.0%~23.4%, and the illness rate of gout, due to the protection of blood-brain barrier, is removed 1%~3%
Outside nerve center, lithate can be deposited on any position of body, urate crystals, and precipitation is deposited on joint, can damage
Hurt joint；By uric acid and urate crystal caused by the oxidative stress caused by renal deposition, inflammatory reaction etc..Short time uric acid
Horizontal sharply increase can lead to acute uric-acid nephropathy (UAN), and long-term uric acid level raising then can lead to chronic urate nephropathy
And uric acid kidney stones, renal function is influenced, the terminal phase can cause uremia；Primary hyperuricemia is frequently accompanied by the high blood of " three high "-
Pressure, hyperlipidemia and diabetes.Since anti-trioxypurine drug has certain liver renal toxicity, the present invention is for anti-trioxypurine and repairs liver kidney
Damage has certain effect.
The sunflower small-molecular peptides extracted through sunflower disk can play anti-inflammatory, detumescence, analgesic and reduction blood uric acid, dissolve uric acid
The effect of sodium, while having good repairing effect to compromised kidneys cell, to high lithemia disease treating both manifestation and root cause of disease, from the high urine of basic treatment
Sour disease；Pueraria lobata includes 12% flavone compound, such as Puerarin, daizeol glycosides, peanut element, has liver protecting effective
The ability of liver cell regeneration is improved, and has effects that lower hyperlipidemia, hypertension, hyperglycemia；Gorgon fruit sweet-puckery flavor is flat, has effects that tonifying spleen is reinforced the kidney, this
Invention is dual-purpose of drug and food, and sunflower disk, Gorgon fruit and pueraria lobata three are shared, and treatment lesions of liver and kidney provides a kind of Nantural non-toxic pair work
Compound can effectively treat gout by anti-trioxypurine and repair the lesions of liver and kidney as caused by hyperuricemia.
Summary of the invention
The present invention provides a kind of sunflower disk, Gorgon fruit and Gegen Compound compositions, are formed using sunflower disk, Gorgon fruit and pueraria lobata
Compound preparation treats gout for anti-trioxypurine, repairs lesions of liver and kidney, solves current existing treatment gout and lesions of liver and kidney drug
Side effect, and can take for a long time to improve kidney-yin deficiency, kidney-yang deficiency the problem of.Present invention demonstrates that Gorgon fruit and pueraria lobata can be auxiliary
It helps enhancing sunflower disk to the repair ability of liver and kidney cells, promotes the reparation of hepatic and renal tissue, reinforce the recovery of hepatic and renal function, three's tool
There is good synergistic effect.
The present invention provides a kind of sunflower disk, Gorgon fruit and Gegen Compound compositions, it is characterised in that be by following raw material by
Made of mass parts ratio:
Sunflower disk: 50~70 parts；Gorgon fruit: 15~25 parts；Pueraria lobata: 15~25 parts.
A kind of sunflower disk, Gorgon fruit and Gegen Compound composition of the present invention, optimum ratio are as follows:
Sunflower disk: 60 parts；Gorgon fruit: 20 parts；Pueraria lobata: 20 parts.
A kind of sunflower disk, Gorgon fruit and Gegen Compound preparation method of composition of the present invention, comprising the following steps:
50~70 parts of step 1) sunflower disk, the water of 10~20 times of quality is added, is added in 0.2% mass fraction after impregnating sufficiently
Property protease in pH6.0~7.0, temperature digest 1~2 hour under the conditions of being 40~50 DEG C, then adjusting pH to 8, in reactor
40~50 DEG C of sufficiently 40~60min of reaction, obtain sunflower disk extracting solution；
10~30 parts of step 2 Gorgon fruit, 70% ethyl alcohol of 10~20 times of quality is added, impregnates abundant 1~2h, flows back and mention in extractor
1~3h is taken, ethyl alcohol is recycled, obtains gorgon fruit extract；
10~30 parts of step 3) pueraria lobata, the water of 10~20 times of quality is added, impregnates abundant 1~2h, refluxing extraction 1 in extractor~
3h obtains root of kudzu vine extract；
Step 4) by steps 1 and 2,3) obtained in extracting solution merge, be concentrated into relative density be 1.10~1.30 clear cream, do
Dry, powder is at fine powder.
Prescription of the present invention is according to as follows:
Prescription of the present invention was recorded with China's Chinese medicine in relation to the Chinese medicine for the treatment of gout and reparation lesions of liver and kidney and in the modern times
Based on theory of medicine, summarized by a large amount of animal experiments and clinical case.
The source of used raw material and effect are as follows in the present invention:
1, sunflower disk, pharmacological property is sweet, nontoxic, trembles with fear, returns kidney channel, records according to " China's book on Chinese herbal medicine ", function is the flat lung of heat-clearing, and analgesic stops
Blood cures mainly headache and dizzy, anti-inflammatory, diuresis, shield kidney etc..
2, Gorgon fruit, pharmacological property are sweet, puckery, flat；Have invigorating the spleen to arrest diarrhea, supplementing the kidney to control the nocturnal, tonifying spleen and stopping diarrhea function.For emission,
Enuresis frequent micturition, spleen deficiency chronic diarrhea, the inferior disease of gonorrhoea band.
3, pueraria lobata, pharmacological property is sweet, pungent, cool.Returns spleen, stomach, lung channel.Expelling pathogenic factors from muscles and skin is brought down a fever, and promoting eruption promotes the production of body fluid to quench thirst, Shengyang Zhixie, promoting menstruation
It is active, relieving alcoholism.It has a headache for fever caused by exogenous pathogens, stiff nape and back is thirsty, it quenches one's thirst, measles without adequate eruption, hot dysentery, diarrhea, dizziness and headache,
Hemiplegia, chest impediment and cardialgia, wine poison wound in.
The positive effect of the present invention and advantage are:
It treats gout and the common drug of high hematuria acid disease is non-steroidal anti-inflammatory drugs, alkaloids medicament and glucocorticoid medicine
Deng being broadly divided into and uric acid inhibited to generate and promote uric acid excretion, said medicine has different degrees of damage to liver kidney, the present invention
With inhibiting uric acid to generate and the double effects of promotion uric acid excretion, compound formulation is made in sunflower disk, Gorgon fruit and pueraria lobata, is utilized
The synergistic effect of three while strengthening kidney repair ability, promotes discharge of the metabolin through kidney, whole comprehensive solution
It has determined and has taken the side effect of said medicine.It is proved through zoopery and clinical treatment, sunflower disk, Gorgon fruit and pueraria lobata compound have
Anti-trioxypurine, treatment gout and the effect for repairing lesions of liver and kidney, and can adhere to taking for a long time.
Detailed description of the invention
Fig. 1, blank control group liver, renal tissue slice；
Fig. 2, model group liver, renal tissue slice；
Fig. 3, positive controls liver, renal tissue slice；
Fig. 4, Semen euryales extract group liver, renal tissue slice
Fig. 5, sunflower disk extract group liver, renal tissue slice；
Fig. 6, kudzu root extract group liver, renal tissue slice；
Fig. 7, sunflower disk of the present invention, 1 group of liver of Gorgon fruit and pueraria lobata compound, renal tissue slice；
Fig. 8, sunflower disk of the present invention, 2 groups of livers of Gorgon fruit and pueraria lobata compound, renal tissue slice；
Fig. 9, sunflower disk of the present invention, 3 groups of livers of Gorgon fruit and pueraria lobata compound, renal tissue slice.
Specific embodiment
It technical solution and is practiced in order to enable those skilled in the art to better understand the present invention, below with reference to specific experiment
The present invention will be further described for example, but lifted experimental example is not as a limitation of the invention.
Embodiment 1
50 parts of step 1) sunflower disk, the water of 10 times of quality is added, the neutral proteinase of 0.2% mass fraction is added after impregnating sufficiently
In pH6.0, temperature digests 1 hour under the conditions of being 40 DEG C, then adjusts pH to 8, and 40 DEG C of sufficiently reaction 40min in reactor are obtained
Sunflower disk extracting solution；
25 parts of step 2 Gorgon fruit, 70% ethyl alcohol of 10 times of quality is added, impregnates abundant 1h, refluxing extraction 1h in extractor recycles second
Alcohol obtains gorgon fruit extract；
25 parts of step 3) pueraria lobata, the water of 10 times of quality is added, impregnates abundant 1h, refluxing extraction 1h in extractor obtains pueraria lobata extraction
Liquid；
Step 4) by steps 1 and 2,3) obtained in extracting solution merge, be concentrated into the clear cream that relative density is 1.10, it is dry, powder at
Fine powder.
Embodiment 2
70 parts of step 1) sunflower disk, the water of 15 times of quality is added, the neutral proteinase of 0.2% mass fraction is added after impregnating sufficiently
In pH6.5, temperature digests 1.5 hours under the conditions of being 45 DEG C, then adjusts pH to 8,45 DEG C of sufficiently reaction 45min in reactor,
Obtain sunflower disk extracting solution；
15 parts of step 2 Gorgon fruit, 70% ethyl alcohol of 15 times of quality is added, impregnates abundant 1.5h, refluxing extraction 2h in extractor, recycling
Ethyl alcohol obtains gorgon fruit extract；
15 parts of step 3) pueraria lobata, the water of 15 times of quality is added, impregnates abundant 1.5h, refluxing extraction 2h in extractor obtains pueraria lobata and mentions
Take liquid；
Step 4) by steps 1 and 2,3) obtained in extracting solution merge, be concentrated into the clear cream that relative density is 1.20, it is dry, powder at
Fine powder.
Embodiment 3
60 parts of step 1) sunflower disk, the water of 20 times of quality is added, the neutral proteinase of 0.2% mass fraction is added after impregnating sufficiently
In pH7.0, temperature digests 2 hours under the conditions of being 50 DEG C, then adjusts pH to 8, and 50 DEG C of sufficiently reaction 50min in reactor are obtained
Sunflower disk extracting solution；
20 parts of step 2 Gorgon fruit, 70% ethyl alcohol of 20 times of quality is added, impregnates abundant 2h, refluxing extraction 3h in extractor recycles second
Alcohol obtains gorgon fruit extract；
20 parts of step 3) pueraria lobata, the water of 20 times of quality is added, impregnates abundant 2h, refluxing extraction 3h in extractor obtains pueraria lobata extraction
Liquid；
Step 4) by steps 1 and 2,3) obtained in extracting solution merge, be concentrated into the clear cream that relative density is 1.20, it is dry, powder at
Fine powder.
The repair that one, or less damages hepatic and renal tissue and hepatic and renal function using the zoopery verifying present invention:
(1) reparation of injury of kidney caused by hyperuricemia
1. experimental animal
SPF grades male mouse of kunming 80,20 ± 2g of weight, the long-living Bioisystech Co., Ltd in Liaoning buys.
2. experiment reagent
Yeast extract (Oxiod company of Britain)；
It corrects SHENBAO HEJI (positive control medicine): Tongyao Pharmaceutical Group Co., Ltd, lot number: 151102；
Semen euryales extract；
Sunflower disk extract；
Kudzu root extract；
1 gained sunflower disk of embodiment, Gorgon fruit and pueraria lobata compound；
2 gained sunflower disk of embodiment, Gorgon fruit and pueraria lobata compound；
3 gained sunflower disk of embodiment, Gorgon fruit and pueraria lobata compound；
3. modeling and medication
Research weighs to mouse before carrying out, wherein 10 are only used as blank control group, remaining mouse is hyperuricemia model
Group.Claim quality between daily morning during experiment.The method that model foundation takes yeast extract stomach-filling is made by giving a large amount of purine substances
Its UA value increases, purine metabolic disturbance, to achieve the effect that temporary raising blood UA value in the short time.80 mouse are random
It is divided into 8 groups, every group about 10, respectively blank control group, model group, positive control (kidney is precious) group, Semen euryales extract group, sunflower
Disk extract group, kudzu root extract group, example 1 group, 2 groups of embodiment, 3 groups of embodiment.During modeling, blank control group is removed
(isometric water stomach-filling) outside, other each group mouse press 20gkg-1 yeast extract daily and carry out stomach-filling, 1 d-1, continuous gavage
20d, carrying out drug stomach-filling to experiment by 200 μ l/d simultaneously from the 9th day terminates, and takes mouse kidney tissue, hepatic and renal tissue is made and cuts
Piece is simultaneously dyed with HE, the variation of microscopically observation histotomy.(Fig. 1-8)
Experimental result is shown in (Fig. 1-8), in blank group (Fig. 1), liver cell and hepatic cell cords are clear in lobuli hepatis, and liver cell cytoplasm is full
It is full；Renal tubular epithelial cytoplasm is not full but normal.In model group (Fig. 2), liver cell and hepatic cell cords in the lobuli hepatis of part
It is unintelligible, the visible dotted necrosis region of liver cell atrophy and liver cell acidophile degeneration；The acidophile degeneration of obvious renal cells,
Renal cells cavitation, breakage, karyopyknosis.Illustrate renal injury model modeling success.In positive controls (Fig. 3),
Liver cell and hepatic cell cords are clear in lobuli hepatis, and liver cell cytoplasm is full, accidental spotty necrosis stove.Slightly renal cells
The acidophile degeneration of cavitation and accidental renal cells.In Semen euryales extract group (Fig. 4), accidental spotty necrosis stove and acidophilia
Become；Renal tubular epithelial cytoplasm is not full but normal.Illustrate that there is good repair to liver, nephridial tissue.Sunflower disk mentions
Take object group, in kudzu root extract group (Fig. 5,6), liver cell and hepatic cell cords are clear in lobuli hepatis, and liver cell cytoplasm is full, accidental
Spotty necrosis stove；The acidophile degeneration of obvious renal cells, renal cells vacuole become, are damaged.Embodiment 1, reality
Apply example 2, liver cell and hepatic cell cords are clear in 3 groups of (Fig. 7-9) lobuli hepatis of embodiment, accidental spotty necrosis stove；Renal tubular epithelial
Cytoplasm is not full, accidental acidophile degeneration and epithelial cell cavitation.And 3 groups of acidophile degenerations of embodiment and cavitation phenomenon mitigation are more aobvious
It writes.Effect most preferably 3 groups of embodiment.
(2) reparation of kidney-yin deficiency caused by hydrocortisone
1. experimental animal
SPF grades of KM mouse: male, weight (20 ± 2) g are provided by No.1 Hospital of Jilin Univ.'s Experimental Animal Center.
2. experiment reagent
Hydrocortisone: MYM BIOLOGICAL TECHNOLOGY COMPANY, lot number: 20160812.
It corrects SHENBAO HEJI (positive control medicine): Tongyao Pharmaceutical Group Co., Ltd, lot number: 151102.
Semen euryales extract；
Sunflower disk extract；
Kudzu root extract；
1 gained sunflower disk of embodiment, Gorgon fruit and pueraria lobata compound；
2 gained sunflower disk of embodiment, Gorgon fruit and pueraria lobata compound；
3 gained sunflower disk of embodiment, Gorgon fruit and pueraria lobata compound；
Rat cortical ketone (Cor.), thyroxine (T4), testosterone (T) Elisa detection kit: Nanjing is built up bioengineering and is ground
Study carefully institute.
3. modeling is administered
It weighs before experiment to mouse, after normal raising three days, carries out modeling experiment, it is primary every weighing in three days, and carry out
Record.
Mouse is divided into 8 groups, every group 10, is respectively labeled as blank group, model group, positive control (kidney is precious) group, Gorgon euryale
Real extract group, sunflower disk extract group, pueraria root extract group, example 1 group, 2 groups of embodiment, 3 groups of embodiment.Blank group 0.2
ML/d stomach-filling distilled water, free water diet 22 days；0.2 mL of model group/d stomach-filling distilled water, free water diet 14
It, beginning in the 15th day 50mg/kg hydrocortisone stomach-filling one week；Remaining each group with 0.2 mL/d continuous gavage 22 days, and
Start while carrying out 50mg/kg hydrocortisone stomach-filling one week within 15th day；22nd day jejunitas next day in night took blood to survey first in serum
The content of shape parathyrine (T4), testosterone (T), cortisone (Cor.).
4. experimental result
The detection of each experimental group biochemical marker level of table 1.:
| Thyroxine (ng/ml) | Testosterone (nmol/L) | Cortisone (ng/ml) | |
| Blank group | 129.76±15.10 | 4.67±0.66 | 257.91±27.66 |
| Model group | 98.74±19.91 | 10.03±0.79 | 173.51±30.04 |
| Kidney treasured group | 107.63±10.77** | 8.05±0.92 | 202.19±19.36** |
| Semen euryales extract | 113.03±22.12** | 8.66±1.29 | 210.17±35.92** |
| Sunflower disk extract | 114.93±10.69** | 7.16±1.18 | 210.49±39.15** |
| Kudzu root extract | 115.81±10.69** | 7.33±1.62 | 210.89±39.42** |
| Embodiment 1 | 117.63±24.51** | 6.34±0.27 | 217.65±29.79** |
| Embodiment 2 | 119.35±27.67** | 6.13±1.08 | 239.17±44.70** |
| Embodiment 3 | 125.73±16.67** | 5.02±0.98 | 248.63±30.27** |
Note: P < 0.01 * P < 0.05 compared with model group, * *.
As shown in Table 1, compared with blank control group, model group thyroxine, corticosterone content are significantly reduced, and testosterone contains
Amount increases, and illustrates kidney-yang deficiency model modeling success.Compared with model group, testosterone levels drop in embodiment group serum in the invention
Low, thyroxine, Corticosterone Level significantly increase (P < 0.01 * P < 0.05, * *), and wherein 3 groups of raising trend of embodiment are more
Obviously, illustrate that sunflower disk, Gorgon fruit and pueraria lobata compound action have synergy, there is better repair to kidney.
(3) reparation of hydrocortisone induced mice kidney-yang deficiency
1. experimental animal
SPF grades of KM mouse: male, weight (20 ± 2) g are provided by No.1 Hospital of Jilin Univ.'s Experimental Animal Center.
2. experiment reagent
Hydrocortisone: MYM BIOLOGICAL TECHNOLOGY COMPANY, lot number: 20160812.
It corrects SHENBAO HEJI (positive control medicine): Tongyao Pharmaceutical Group Co., Ltd, lot number: 151102.
Semen euryales extract；
Sunflower disk extract；
Kudzu root extract；
1 gained sunflower disk of embodiment, Gorgon fruit and pueraria lobata compound；
2 gained sunflower disk of embodiment, Gorgon fruit and pueraria lobata compound；
3 gained sunflower disk of embodiment, Gorgon fruit and pueraria lobata compound；
Rat cortical ketone (Cor.), thyroxine (T4), testosterone (T) Elisa detection kit: Nanjing is built up bioengineering and is ground
Study carefully institute.
3. modeling is administered
It weighs before experiment to mouse, after normal raising three days, carries out modeling experiment, it is primary every weighing in three days, and carry out
Record.Mouse is divided into 8 groups, every group 10, is respectively labeled as blank group, model group, positive control (kidney is precious) group, Gorgon fruit mention
Take object group, sunflower disk extract group, example 1 group, 2 groups of embodiment, 3 groups of embodiment.0.2 mL of blank group/d stomach-filling distillation
Water, free water diet；0.2 mL of model group/d stomach-filling distilled water, free water diet 14 days, the 15th day beginning 25mg/kg
Hydrocortisone stomach-filling 10 days；Remaining each group is with 0.2 mL/d continuous gavage 25 days, and the 15th day starts while carrying out
25mg/kg hydrocortisone stomach-filling 10 days；25th day jejunitas next day in night take blood survey serum in thyroxine (T4), testosterone (T),
The content of cortisone (Cor.).
4. experimental result
The detection of each experimental group biochemical marker level of table 2.:
| Thyroxine (ng/ml) | Testosterone (nmol/L) | Cortisone (ng/ml) | |
| Blank group | 56.54±9.79 | 4.03±0.93 | 160.93±37.91 |
| Model group | 112.34±19.95 | 3.74±0.82 | 249.74±31.23 |
| Kidney treasured group | 63.65±7.63** | 5.13±0.33 | 162.81±24.98** |
| Semen euryales extract | 67.73±10.51** | 5.92±1.24 | 173.62±33.28** |
| Sunflower disk extract | 63.18±11.97** | 5.87±0.16 | 170.39±45.66** |
| Kudzu root extract | 62.65±11.24** | 5.63±0.18 | 170.12±45.42** |
| Embodiment 1 | 61.91±13.49** | 5.19±0.91 | 168.88±22.43** |
| Embodiment 2 | 60.14±9.22** | 4.78±0.88 | 168.94±34.19** |
| Embodiment 3 | 58.39±6.75** | 4.62±0.31 | 165.15±30.08** |
Note: P < 0.01 * P < 0.05 compared with model group, * *.
As shown in Table 1, compared with blank control group, model group testosterone concentration is reduced, thyroxine, and corticosterone content is aobvious
It writes and increases, illustrate Kidney-yin-vacuity modeling success.Compared with model group, testosterone levels liter in embodiment group serum in the invention
Height, thyroxine, Corticosterone Level significantly reduce (P < 0.01 * P < 0.05, * *), and wherein 3 groups of reductions of embodiment are more bright
It is aobvious, illustrate that sunflower disk, Gorgon fruit and pueraria lobata compound action have synergy, there is better repair to kidney.
Conclusion: sunflower disk, Gorgon fruit and Gegen Compound extract treat gout in anti-trioxypurine and repair liver, kidney in the present invention
Application in damage is experimentally confirmed with good repair, and sunflower disk, Gorgon fruit and pueraria lobata are used in combination, more singly
Solely using having more obvious effect, three has synergistic effect, can significantly increase its reparation to impaired liver, kidney.And because
Sunflower disk, Gorgon fruit and pueraria lobata have the characteristics that the natural without side-effects of dual-purpose of drug and food, and three's synergistic effect can reinforce liver, kidney group
It knits and the reparation of function, and provide effective method and theoretical foundation to take treatment for a long time.
Two, further demonstrate that curative effect of the invention by following typical case
Case 1
Patient Zhang, male, 60 one full year of life, gout many years Relapse rate, blood uric acid value reach 700umol/L, serum creatinine 127umol/L.
Taking folk prescription sunflower disk, blood uric acid value reduces and stablizes in 400umol/L after three weeks, but has a dizzy spell, vexed to sleep less, even
See heating paresthesia, consider patient's gout for many years, serum creatinine level is higher, uric acid in blood largely accumulation metabolism for a long time, to kidney
Serious burden is caused, renal function is damaged, and changes and takes compound of the present invention after one month, and serum uric acid level is stablized
While 370umol/L, serum creatinine drops to 90umol/L, and it is steady to detect blood uric acid, serum creatinine level after deactivating one month
It is fixed, then without recurrence.
Case 2
Patient's Mr. Wang, male, 37 one full year of life find that blood uric acid value is higher when last year physical examination, and hospital's detection serum uric acid level reaches
490umol/L, the horizontal 100umol/L of serum creatinine.Take folk prescription sunflower disk after two weeks, serum uric acid level is down to 340umol/L, blood
Creatinine level 102umol/L often has lassitude fatiguability, the symptoms such as edema from soreness and weakness of waist and knees morning, but since patient makees for a long time
It is irregular to cease diet, considers patient's kidney deficiency constitution, after this takes compound two weeks of the present invention, serum uric acid level is stablized
340umol/L, serum creatinine horizontal stable decrease in 90umol/L, serum creatinine level, and patient's spiritlessness and weakness phenomenon is delayed
Solution, without recurrence after deactivated half a year.
Case 3
Patient Lee, male, 51 one full year of life suffer from more than ten years of hyperuricemia, last year concurrent gout symptom, blood uric acid value 516umol/L,
Serum creatinine 130umol/L.After taking folk prescription sunflower disk two weeks, blood uric acid value is down to 390umol/L, serum creatinine 128umol/L,
And with long-term soreness and weakness of waist and knees, dizziness and tinnitus symptom considers that patient's past more than ten years long-term blood uric acid is higher, causes to kidney
Damage, used compound of the present invention instead after one month, and blood uric acid value is stablized in 380umol/L, and serum creatinine horizontal stable exists
87umol/L, soreness and weakness of waist and knees, dizziness and tinnitus symptom disappear, illustrate to repair renal function it is good, it is three months deactivated after further consultation without
Recurrence.
A series of listed detailed descriptions illustrating only for feasible embodiment of the present invention, they are simultaneously
It is non-to limit the scope of the invention, it is all to be wrapped without departing from equivalent implementations made by essence of the invention or change
Containing within protection scope of the present invention.
Claims (3)
1. a kind of sunflower disk Gorgon fruit and Gegen Compound composition, it is characterised in that be made of following raw material by mass parts ratio
:
Sunflower disk: 50~70 parts；Gorgon fruit: 15~25 parts；Pueraria lobata: 15~25 parts.
2. a kind of sunflower disk Gorgon fruit as described in claim 1 and Gegen Compound composition, optimum ratio are as follows:
Sunflower disk: 60 parts；Gorgon fruit: 20 parts；Pueraria lobata: 20 parts.
3. a kind of sunflower disk Gorgon fruit as claimed in claim 1 or 2 and Gegen Compound preparation method of composition, including following step
It is rapid:
50~70 parts of step 1) sunflower disk, the water of 10~20 times of quality is added, is added in 0.2% mass fraction after impregnating sufficiently
Property protease in pH6.0~7.0, temperature digest 1~2 hour under the conditions of being 40~50 DEG C, then adjusting pH to 8, in reactor
40~50 DEG C of sufficiently 40~60min of reaction, obtain sunflower disk extracting solution；
10~30 parts of step 2 Gorgon fruit, 70% ethyl alcohol of 10~20 times of quality is added, impregnates abundant 1~2h, flows back and mention in extractor
1~3h is taken, ethyl alcohol is recycled, obtains gorgon fruit extract；
10~30 parts of step 3) pueraria lobata, the water of 10~20 times of quality is added, impregnates abundant 1~2h, refluxing extraction 1 in extractor~
3h obtains root of kudzu vine extract；
Step 4) by steps 1 and 2,3) obtained in extracting solution merge, be concentrated into relative density be 1.10~1.30 clear cream, do
Dry, powder is at fine powder.
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| CN201811509838.8A CN109260266A (en) | 2018-12-11 | 2018-12-11 | The purposes of sunflower disk Gorgon fruit and pueraria lobata compound in anti-trioxypurine reparation lesions of liver and kidney |
| AU2019100730A AU2019100730A4 (en) | 2018-12-11 | 2019-07-03 | Use of Composition of Sunflower Head, Gorgon Euryale Seed and Kudzuvine Root in Lowering Uric Acid and Repair of Liver and Kidney Injury |
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| CN201811509838.8A CN109260266A (en) | 2018-12-11 | 2018-12-11 | The purposes of sunflower disk Gorgon fruit and pueraria lobata compound in anti-trioxypurine reparation lesions of liver and kidney |
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|---|---|---|---|---|
| CN109045224A (en) * | 2018-10-29 | 2018-12-21 | 吉林大学 | A kind of sunflower disk semen coicis compound and the purposes in treatment hepatic injury |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108354956A (en) * | 2018-03-08 | 2018-08-03 | 吉林大学 | Sunflower disk powder and its active ingredient are preparing the application in repairing wounded hepatocytes drug |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108354956A (en) * | 2018-03-08 | 2018-08-03 | 吉林大学 | Sunflower disk powder and its active ingredient are preparing the application in repairing wounded hepatocytes drug |
Non-Patent Citations (1)
| Title |
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| 戴惠咛等: "葵花盘粉有效成分对小鼠高尿酸血症的治疗作用", 《吉林大学学报（医学版）》 * |
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| CN109045224A (en) * | 2018-10-29 | 2018-12-21 | 吉林大学 | A kind of sunflower disk semen coicis compound and the purposes in treatment hepatic injury |
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Application publication date: 20190125 |