AU2018352722A1 - Devices, systems, and methods for high throughput single cell analysis - Google Patents

Devices, systems, and methods for high throughput single cell analysis Download PDF

Info

Publication number
AU2018352722A1
AU2018352722A1 AU2018352722A AU2018352722A AU2018352722A1 AU 2018352722 A1 AU2018352722 A1 AU 2018352722A1 AU 2018352722 A AU2018352722 A AU 2018352722A AU 2018352722 A AU2018352722 A AU 2018352722A AU 2018352722 A1 AU2018352722 A1 AU 2018352722A1
Authority
AU
Australia
Prior art keywords
weir
trap
cell
microfluidic device
traps
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2018352722A
Other languages
English (en)
Inventor
Ying Li
Jeff MOSTSCHMAN
Benjamin Yellen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Duke University
Original Assignee
Duke University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Duke University filed Critical Duke University
Publication of AU2018352722A1 publication Critical patent/AU2018352722A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles or throttle valves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Fluid Mechanics (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2018352722A 2017-10-20 2018-10-17 Devices, systems, and methods for high throughput single cell analysis Abandoned AU2018352722A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762574865P 2017-10-20 2017-10-20
US62/574,865 2017-10-20
PCT/US2018/056221 WO2019079399A1 (en) 2017-10-20 2018-10-17 DEVICES, SYSTEMS AND METHODS FOR HIGH SPEED SINGLE CELL ANALYSIS

Publications (1)

Publication Number Publication Date
AU2018352722A1 true AU2018352722A1 (en) 2020-03-26

Family

ID=66174645

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2018352722A Abandoned AU2018352722A1 (en) 2017-10-20 2018-10-17 Devices, systems, and methods for high throughput single cell analysis

Country Status (7)

Country Link
US (1) US20210114029A1 (cg-RX-API-DMAC10.html)
EP (1) EP3697536A4 (cg-RX-API-DMAC10.html)
JP (1) JP2021500012A (cg-RX-API-DMAC10.html)
CN (1) CN111886074A (cg-RX-API-DMAC10.html)
AU (1) AU2018352722A1 (cg-RX-API-DMAC10.html)
CA (1) CA3075512A1 (cg-RX-API-DMAC10.html)
WO (1) WO2019079399A1 (cg-RX-API-DMAC10.html)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016191533A1 (en) 2015-05-26 2016-12-01 The Trustees Of Columbia University In The City Of New York Rna printing and sequencing devices, methods, and systems
EP3717661A1 (en) 2017-11-27 2020-10-07 The Trustees of Columbia University in the City of New York Rna printing and sequencing devices, methods, and systems
US11890616B2 (en) * 2019-03-26 2024-02-06 The Curators Of The University Of Missouri Microfluidic device for capture of micrometer scale objects and methods of using the device
US20200394459A1 (en) * 2019-06-17 2020-12-17 Nvidia Corporation Cell image synthesis using one or more neural networks
CN110283724B (zh) * 2019-07-09 2022-07-29 汇芯(北京)科技有限公司 一种高通量类器官表型筛查系统和方法
WO2021011895A1 (en) * 2019-07-18 2021-01-21 Celldom, Inc. Methods and devices for single cell barcoding
WO2021163442A1 (en) * 2020-02-14 2021-08-19 Chan Zuckerberg Biohub, Inc. Microfluidic cell barcoding and sequencing
EP4105340A4 (en) * 2020-02-14 2023-08-09 Sony Group Corporation ANALYTICAL METHOD, ANALYTICAL SYSTEM AND INTERFACE FOR ANALYSIS
US11900595B2 (en) 2020-05-08 2024-02-13 Singular Genomics Systems, Inc. Imaging systems and methods useful for patterned structures
DE102020004660B4 (de) * 2020-07-31 2022-06-15 Evorion Biotechnologies Gmbh Vorrichtung zur sequentiellen Positionierung von Partikeln
CN117529659A (zh) * 2021-03-26 2024-02-06 Essenlix 公司 细胞和其它生物实体分析和计数
CN113607609B (zh) * 2021-06-01 2022-08-16 武汉大学 基于指压式微流控平台的智能手机成像分析系统及其应用
US12257580B2 (en) * 2021-09-30 2025-03-25 Water Bear Health Technology (Nantong) Co., LTD. Microfluidic chip and manufacture method thereof, and cell separation and single-cell western blotting method
WO2023144192A1 (de) * 2022-01-25 2023-08-03 Evorion Biotechnologies Gmbh Vorrichtung zur sequentiellen positionierung von partikeln
DE102022125010A1 (de) * 2022-09-28 2024-03-28 Leibniz-Institut für Festkörper- und Werkstoffforschung Dresden e.V. (IFW Dresden e.V.) Mikrofluidisches Bauteil
US20240198336A1 (en) 2022-12-19 2024-06-20 Anchorline Biolabs, Inc. Capillary partitioning microfluidics

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102665847A (zh) * 2009-12-25 2012-09-12 学校法人常翔学园 具有固液分离功能的装置、μ-TAS设备及固液分离方法
JP5777042B2 (ja) * 2010-10-01 2015-09-09 国立大学法人 東京大学 受精卵培養装置、受精卵培養方法
CA2842359A1 (en) 2011-08-01 2013-02-07 Denovo Sciences Cell capture system and method of use
US9957472B2 (en) * 2011-09-22 2018-05-01 Georgia Tech Research Corporation Deterministic high-density single-cell trap array
JP2016527494A (ja) * 2013-07-05 2016-09-08 ユニバーシティ オブ ワシントン スルー イッツ センター フォー コマーシャライゼーション マイクロ流体アッセイのための方法、組成物およびシステム
US9757727B2 (en) * 2014-05-29 2017-09-12 Massachusetts Institute Of Technology Hydrodynamic trap array
KR102195769B1 (ko) * 2014-07-10 2020-12-30 주식회사 미코바이오메드 미세유체 칩, 이의 제조 방법 및 이를 이용한 분석 장치
WO2017123697A1 (en) * 2016-01-13 2017-07-20 Duke University Platforms for single cell analysis
EP3442707B1 (en) 2016-04-15 2023-10-04 President and Fellows of Harvard College Systems for the collection of droplets and/or other entities

Also Published As

Publication number Publication date
US20210114029A1 (en) 2021-04-22
EP3697536A1 (en) 2020-08-26
WO2019079399A1 (en) 2019-04-25
WO2019079399A8 (en) 2019-05-16
CA3075512A1 (en) 2019-04-25
CN111886074A (zh) 2020-11-03
JP2021500012A (ja) 2021-01-07
EP3697536A4 (en) 2021-07-14

Similar Documents

Publication Publication Date Title
US20210114029A1 (en) Devices, systems, and methods for high throughput single cell analysis
US11618024B2 (en) Manipulation of fluids, fluid components and reactions in microfluidic systems
AU2024203580B2 (en) System and Method for Isolating and Analyzing Cells
US12325022B2 (en) Microfluidic device, system, and method for the study of organisms
CN107407691B (zh) 用于单细胞中核酸靶标的分子条码化的装置和系统
WO2021011895A1 (en) Methods and devices for single cell barcoding
US20140308207A1 (en) Open microfluidic devices for chemotaxis, methods of using same, and applications of same
Wang et al. Automated parallel cell isolation and deposition using microwell array and optical tweezers
Verron et al. Isolation of individual natural killer cells from deep, high-aspect ratio microwell arrays-an evaluative study
Chingozha New microfluidic systems for single-cell signaling studies in immune cells

Legal Events

Date Code Title Description
MK5 Application lapsed section 142(2)(e) - patent request and compl. specification not accepted