AU2018313286B2 - Digital amplification with primers of limited nucleotide composition - Google Patents

Digital amplification with primers of limited nucleotide composition Download PDF

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Publication number
AU2018313286B2
AU2018313286B2 AU2018313286A AU2018313286A AU2018313286B2 AU 2018313286 B2 AU2018313286 B2 AU 2018313286B2 AU 2018313286 A AU2018313286 A AU 2018313286A AU 2018313286 A AU2018313286 A AU 2018313286A AU 2018313286 B2 AU2018313286 B2 AU 2018313286B2
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primer
nucleic acid
primers
target nucleic
underrepresented
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AU2018313286A1 (en
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Xin Chen
Rong Wang
Youxiang Wang
Zhijie Yang
Yu Zhao
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Atila BioSystems Inc
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Atila BioSystems Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/10—Characterised by chemical treatment
    • C12Q2523/125—Bisulfite(s)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/185—Modifications characterised by incorporating bases where the precise position of the bases in the nucleic acid string is important
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/16—Assays for determining copy number or wherein the copy number is of special importance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2018313286A 2017-08-11 2018-08-10 Digital amplification with primers of limited nucleotide composition Active AU2018313286B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762544605P 2017-08-11 2017-08-11
US62/544,605 2017-08-11
PCT/US2018/046360 WO2019033065A1 (en) 2017-08-11 2018-08-10 DIGITAL AMPLIFICATION WITH LIMITED NUCLEOTIDE COMPOSITION PRIMERS

Publications (2)

Publication Number Publication Date
AU2018313286A1 AU2018313286A1 (en) 2020-03-12
AU2018313286B2 true AU2018313286B2 (en) 2024-09-12

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AU2018313286A Active AU2018313286B2 (en) 2017-08-11 2018-08-10 Digital amplification with primers of limited nucleotide composition

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US (1) US11739375B2 (enExample)
EP (1) EP3665297A4 (enExample)
JP (1) JP7537746B2 (enExample)
CN (1) CN111183229A (enExample)
AU (1) AU2018313286B2 (enExample)
CA (1) CA3072591A1 (enExample)
WO (1) WO2019033065A1 (enExample)

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CN114250274A (zh) * 2015-04-24 2022-03-29 阿提拉生物系统公司 利用有限核苷酸组成的引物扩增
AU2018313286B2 (en) 2017-08-11 2024-09-12 Atila Biosystems Incorporated Digital amplification with primers of limited nucleotide composition
CN109666747A (zh) * 2019-02-20 2019-04-23 天津脉络医学检验有限公司 一种引物探针组合物及其应用
IT201900006679A1 (it) * 2019-05-09 2020-11-09 Artemisia S P A METODO PER LA DETERMINAZIONE DIRETTA DI ANEUPLOIDIE A CARICO DEL FETO DA ANALISI NON INVASIVA DEL DNA FETALE DA SANGUE MATERNO MEDIANTE dPCR
CN110656167A (zh) * 2019-09-27 2020-01-07 中国科学院苏州生物医学工程技术研究所 用于y染色体微缺失检测的引物组、试剂盒及基于数字pcr的检测方法
CN110904182B (zh) * 2019-11-19 2023-08-18 中国科学院深圳先进技术研究院 基因组重复序列删除率确定方法、装置、设备及存储介质
CN111500788B (zh) * 2020-05-29 2023-11-07 领航医学科技(深圳)有限公司 用于人类疱疹病毒感染检测的试剂盒及其检测方法
CN112899346A (zh) * 2021-01-05 2021-06-04 南京普济生物有限公司 一种降低pcr非特异性扩增的核苷酸及其设计方法与应用
CN112899366A (zh) * 2021-01-06 2021-06-04 南京普济生物有限公司 一种结直肠癌基因突变位点的检测方法及其试剂盒
CN113960142B (zh) * 2021-10-25 2023-11-10 福州大学 一种回文核酸纳米片超灵敏电化学生物传感器的制备方法
CN113789389A (zh) * 2021-11-18 2021-12-14 广东永诺医疗科技有限公司 人白血病bcr-abl融合突变一步法检测试剂盒
CN114196740A (zh) * 2021-12-09 2022-03-18 南京普济生物有限公司 用于同时识别多种基因类型的数字扩增检测方法、检测产品和检测试剂盒
WO2024081873A1 (en) * 2022-10-14 2024-04-18 The Board Of Trustees Of The Leland Stanford Junior University Methods for evaluating health and stability of cultured cells

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DE10236406C1 (de) 2002-08-02 2003-12-24 Epigenomics Ag Verfahren zur Amplifikation von Nukleinsäuren mit geringer Komplexität
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KR20100019220A (ko) 2008-08-08 2010-02-18 삼성전자주식회사 열순환을 포함하는 다중 치환 증폭에 의하여 표적 핵산 서열을 증폭하는 방법
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AU2018313286B2 (en) 2017-08-11 2024-09-12 Atila Biosystems Incorporated Digital amplification with primers of limited nucleotide composition

Also Published As

Publication number Publication date
US20200165668A1 (en) 2020-05-28
JP7537746B2 (ja) 2024-08-21
EP3665297A4 (en) 2021-05-12
AU2018313286A1 (en) 2020-03-12
US11739375B2 (en) 2023-08-29
CN111183229A (zh) 2020-05-19
EP3665297A1 (en) 2020-06-17
WO2019033065A1 (en) 2019-02-14
CA3072591A1 (en) 2019-02-14
JP2020532964A (ja) 2020-11-19

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