AU2018313286B2 - Digital amplification with primers of limited nucleotide composition - Google Patents

Digital amplification with primers of limited nucleotide composition Download PDF

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Publication number
AU2018313286B2
AU2018313286B2 AU2018313286A AU2018313286A AU2018313286B2 AU 2018313286 B2 AU2018313286 B2 AU 2018313286B2 AU 2018313286 A AU2018313286 A AU 2018313286A AU 2018313286 A AU2018313286 A AU 2018313286A AU 2018313286 B2 AU2018313286 B2 AU 2018313286B2
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primer
nucleic acid
primers
target nucleic
underrepresented
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AU2018313286A1 (en
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Xin Chen
Rong Wang
Youxiang Wang
Zhijie Yang
Yu Zhao
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Atila BioSystems Inc
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Atila BioSystems Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/10—Characterised by chemical treatment
    • C12Q2523/125—Bisulfite(s)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/185—Modifications characterised by incorporating bases where the precise position of the bases in the nucleic acid string is important
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/16—Assays for determining copy number or wherein the copy number is of special importance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2018313286A 2017-08-11 2018-08-10 Digital amplification with primers of limited nucleotide composition Active AU2018313286B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762544605P 2017-08-11 2017-08-11
US62/544,605 2017-08-11
PCT/US2018/046360 WO2019033065A1 (en) 2017-08-11 2018-08-10 DIGITAL AMPLIFICATION WITH LIMITED NUCLEOTIDE COMPOSITION PRIMERS

Publications (2)

Publication Number Publication Date
AU2018313286A1 AU2018313286A1 (en) 2020-03-12
AU2018313286B2 true AU2018313286B2 (en) 2024-09-12

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AU2018313286A Active AU2018313286B2 (en) 2017-08-11 2018-08-10 Digital amplification with primers of limited nucleotide composition

Country Status (7)

Country Link
US (1) US11739375B2 (enExample)
EP (1) EP3665297A4 (enExample)
JP (1) JP7537746B2 (enExample)
CN (1) CN111183229A (enExample)
AU (1) AU2018313286B2 (enExample)
CA (1) CA3072591A1 (enExample)
WO (1) WO2019033065A1 (enExample)

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ES2961374T3 (es) * 2015-04-24 2024-03-11 Atila Biosystems Incorporated Amplificación con cebadores de composición de nucleótidos limitada
US11739375B2 (en) 2017-08-11 2023-08-29 Atila Biosystems Incorporated Digital amplification with primers of limited nucleotide composition
CN109666747A (zh) * 2019-02-20 2019-04-23 天津脉络医学检验有限公司 一种引物探针组合物及其应用
IT201900006679A1 (it) * 2019-05-09 2020-11-09 Artemisia S P A METODO PER LA DETERMINAZIONE DIRETTA DI ANEUPLOIDIE A CARICO DEL FETO DA ANALISI NON INVASIVA DEL DNA FETALE DA SANGUE MATERNO MEDIANTE dPCR
CN110656167A (zh) * 2019-09-27 2020-01-07 中国科学院苏州生物医学工程技术研究所 用于y染色体微缺失检测的引物组、试剂盒及基于数字pcr的检测方法
CN110904182B (zh) * 2019-11-19 2023-08-18 中国科学院深圳先进技术研究院 基因组重复序列删除率确定方法、装置、设备及存储介质
CN111500788B (zh) * 2020-05-29 2023-11-07 领航医学科技(深圳)有限公司 用于人类疱疹病毒感染检测的试剂盒及其检测方法
CN112899346A (zh) * 2021-01-05 2021-06-04 南京普济生物有限公司 一种降低pcr非特异性扩增的核苷酸及其设计方法与应用
CN112899366A (zh) * 2021-01-06 2021-06-04 南京普济生物有限公司 一种结直肠癌基因突变位点的检测方法及其试剂盒
US20260062748A1 (en) * 2021-09-02 2026-03-05 Countable Labs, Inc. Detection and digital quantitation of multiple targets
CN113960142B (zh) * 2021-10-25 2023-11-10 福州大学 一种回文核酸纳米片超灵敏电化学生物传感器的制备方法
CN113789389A (zh) * 2021-11-18 2021-12-14 广东永诺医疗科技有限公司 人白血病bcr-abl融合突变一步法检测试剂盒
CN114196740A (zh) * 2021-12-09 2022-03-18 南京普济生物有限公司 用于同时识别多种基因类型的数字扩增检测方法、检测产品和检测试剂盒
WO2024081873A1 (en) * 2022-10-14 2024-04-18 The Board Of Trustees Of The Leland Stanford Junior University Methods for evaluating health and stability of cultured cells
CN117816262A (zh) * 2024-01-05 2024-04-05 湖南工程学院 一种检测癌症突变基因的一步式微流控微珠阵列芯片、试剂盒及其应用
CN118853760A (zh) * 2024-07-15 2024-10-29 首都医科大学附属北京天坛医院 一种构建Map3k3I441M突变敲入转基因小鼠模型的方法及应用

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US7387876B2 (en) 2004-02-27 2008-06-17 President And Fellows Of Harvard College Amplification of trace amounts of nucleic acids
US8871469B1 (en) 2004-11-13 2014-10-28 Steven Albert Benner Self-avoiding molecular recognition systems in DNA priming
CN101111606B (zh) 2004-12-03 2012-05-16 人类遗传标记控股有限公司 通过化学修饰胞嘧啶而简化微生物核酸的方法
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KR20100019220A (ko) 2008-08-08 2010-02-18 삼성전자주식회사 열순환을 포함하는 다중 치환 증폭에 의하여 표적 핵산 서열을 증폭하는 방법
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Also Published As

Publication number Publication date
CN111183229A (zh) 2020-05-19
WO2019033065A1 (en) 2019-02-14
JP7537746B2 (ja) 2024-08-21
EP3665297A4 (en) 2021-05-12
CA3072591A1 (en) 2019-02-14
EP3665297A1 (en) 2020-06-17
AU2018313286A1 (en) 2020-03-12
US11739375B2 (en) 2023-08-29
US20200165668A1 (en) 2020-05-28
JP2020532964A (ja) 2020-11-19

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