AU2018101446A4 - A preparation of anti-cancer fungi combined with traditional Chinese medicine and it's preparation method - Google Patents
A preparation of anti-cancer fungi combined with traditional Chinese medicine and it's preparation method Download PDFInfo
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- AU2018101446A4 AU2018101446A4 AU2018101446A AU2018101446A AU2018101446A4 AU 2018101446 A4 AU2018101446 A4 AU 2018101446A4 AU 2018101446 A AU2018101446 A AU 2018101446A AU 2018101446 A AU2018101446 A AU 2018101446A AU 2018101446 A4 AU2018101446 A4 AU 2018101446A4
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- 239000003814 drug Substances 0.000 title claims description 13
- 238000002360 preparation method Methods 0.000 title abstract description 10
- 241000233866 Fungi Species 0.000 title abstract description 6
- 230000001093 anti-cancer Effects 0.000 title description 2
- 241000222355 Trametes versicolor Species 0.000 claims abstract description 10
- 235000006533 astragalus Nutrition 0.000 claims abstract description 8
- 241000045403 Astragalus propinquus Species 0.000 claims abstract description 5
- 241000830535 Ligustrum lucidum Species 0.000 claims abstract description 5
- 230000000694 effects Effects 0.000 claims description 11
- 239000004615 ingredient Substances 0.000 claims description 10
- 206010058467 Lung neoplasm malignant Diseases 0.000 claims description 6
- 206010060862 Prostate cancer Diseases 0.000 claims description 6
- 208000000236 Prostatic Neoplasms Diseases 0.000 claims description 6
- 201000005202 lung cancer Diseases 0.000 claims description 6
- 208000020816 lung neoplasm Diseases 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 230000022131 cell cycle Effects 0.000 claims description 5
- 241000735234 Ligustrum Species 0.000 claims description 4
- 241001061264 Astragalus Species 0.000 claims description 3
- 210000004233 talus Anatomy 0.000 claims description 3
- 206010028980 Neoplasm Diseases 0.000 abstract description 9
- 201000011510 cancer Diseases 0.000 abstract description 9
- 235000008216 herbs Nutrition 0.000 abstract description 3
- 238000000034 method Methods 0.000 abstract description 2
- 150000004676 glycans Chemical class 0.000 description 4
- 229920001282 polysaccharide Polymers 0.000 description 4
- 239000005017 polysaccharide Substances 0.000 description 4
- 238000000605 extraction Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- MIJYXULNPSFWEK-GTOFXWBISA-N 3beta-hydroxyolean-12-en-28-oic acid Chemical compound C1C[C@H](O)C(C)(C)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@@]5(C(O)=O)CCC(C)(C)C[C@H]5C4=CC[C@@H]3[C@]21C MIJYXULNPSFWEK-GTOFXWBISA-N 0.000 description 1
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- JKLISIRFYWXLQG-UHFFFAOYSA-N Epioleonolsaeure Natural products C1CC(O)C(C)(C)C2CCC3(C)C4(C)CCC5(C(O)=O)CCC(C)(C)CC5C4CCC3C21C JKLISIRFYWXLQG-UHFFFAOYSA-N 0.000 description 1
- 241000220485 Fabaceae Species 0.000 description 1
- 230000035519 G0 Phase Effects 0.000 description 1
- 230000010190 G1 phase Effects 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 206010027476 Metastases Diseases 0.000 description 1
- 241000207834 Oleaceae Species 0.000 description 1
- YBRJHZPWOMJYKQ-UHFFFAOYSA-N Oleanolic acid Natural products CC1(C)CC2C3=CCC4C5(C)CCC(O)C(C)(C)C5CCC4(C)C3(C)CCC2(C1)C(=O)O YBRJHZPWOMJYKQ-UHFFFAOYSA-N 0.000 description 1
- MIJYXULNPSFWEK-UHFFFAOYSA-N Oleanolinsaeure Natural products C1CC(O)C(C)(C)C2CCC3(C)C4(C)CCC5(C(O)=O)CCC(C)(C)CC5C4=CCC3C21C MIJYXULNPSFWEK-UHFFFAOYSA-N 0.000 description 1
- 229920001231 Polysaccharide peptide Polymers 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 238000009098 adjuvant therapy Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 235000011869 dried fruits Nutrition 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000012869 ethanol precipitation Methods 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 235000021374 legumes Nutrition 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 230000009401 metastasis Effects 0.000 description 1
- 229940100243 oleanolic acid Drugs 0.000 description 1
- 108010022457 polysaccharide peptide Proteins 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- HZLWUYJLOIAQFC-UHFFFAOYSA-N prosapogenin PS-A Natural products C12CC(C)(C)CCC2(C(O)=O)CCC(C2(CCC3C4(C)C)C)(C)C1=CCC2C3(C)CCC4OC1OCC(O)C(O)C1O HZLWUYJLOIAQFC-UHFFFAOYSA-N 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
Abstract
The disclosure relates to a combination of medical fungi and Chinese herbs, its method of preparation and its use in the treatment of cancer. The combination is composed of Trametes versicolor mycelia PSP, Astragalus membranaceus extract and Ligustrum lucidum extract.
Description
A preparation of anti-cancer fungi combined with traditional Chinese medicine and it's preparation method
Cancer is a big issue related to human health. Currently there are medical fungi or Chinese herbs used alone as adjuvant to treat cancer respectively. The invention discloses a preparation combining the ingredients of medical fungi and Chinese herbs and their making method to get a better effect of adjuvant treatment and improve its action mechanism.
The preparation of the invention is composed of Trametes versicolor mycelia PSP, Astragalus membranaceus extract and Ligustrum lucidum extract(referred to as Mix) .Their composition ratio is Trametes versicolor mycelia PSP 3.0 , Astragalus membranaceus extract 2.0, Ligustrum lucidum extract 1.0, (Calculated by weight). Trametes versicolor (also as Coriolus versicolor, Yun Zi or Turkey tail) is a medicine fungi which belong to the Polypore mushroom. Commercially available raw material is Trametes Versicolor's Mycelium extract PSP, which is one of the ingredients of this preparation, mainly containing polysaccharide peptide PSP and polysaccharide. The content of the peptide is >20.0%, and the content of polysaccharide is >25.0%. Both are active ingredients of Trametes Versicolor obtained by deep-cultured of Trametes Versicolor mycelia and hot water extraction then ethanol precipitation. The quality complies with Chinese National Drug Standards. Astragalus Membranaceus belong in the family of Fabaceae. It contains Astragalus Polysaccharides, flavonoids and trace elements. Its solid extract can be obtained from the market. The extraction ratio is 10:1.
2018101446 21 Nov 2018
Ligustrum Lucidum is an Oleaceae plant. Its dried fruit are called Nu Zhen Zi in traditional Chinese medicine. It contains oleanolic acid, mannitol and polysaccharides, and is used to nourish liver and kidney. Its extract can be obtained from the market. The extraction ratio is 10:1. Both raw materials Astragalus and Ligustrum are in accordance with the Chinese Pharmacopoeia.
Prostate cancer cells lines (PC-3 and NCaP) and lung cancer cells lines (A549 and H1975) were cultured alone or in combination with the above three components, and their DNA content was lower than that of the untreated control group (P<0.05). Therefore, the preparation of the present invention inhibits the synthesis of cancer cell DNA and inhibits the growth and proliferation of cancer cells. The effect on the cell cycle suggests that increasing cancer cell arrest in the G0/G1 phase blocks GO cancer cells from entering the active phase or preventing cell division. Thereby, cancer patients who have completed treatment have demonstrated the effect of assisting rehabilitation and prevention of cancer recurrence and metastasis. The three components in the preparation of the invention have a synergistic effect, and the efficacy is 1-2 times better than the individual components.
Claims (8)
- ClainThe preperation is composed of Trametes versicolor mycelia PSP, Astragalus membranaceus atiorepextract and Ligustrum lucidum extract1/81. Versicolor
- 2. Astragallus2018101446 21 Nov 20181.prostate cancerPC-3Dose (mg/mL)Baseline * p<0.05 compared to ControlFig. 1 The effect of Chinese medicine ingredients on DNA content in prostate cancer cell lines PC-32/82018101446 21 Nov 2018PC-31.2k see ecc300Mixture200 400 SOO SQO l.OK
Gq/G! 59.8% ± 3.9% 60.3% ± 3.9% 67.4% ± 0.4% 61.8% ± 5.3% S 14.0% ± 2.6% 16.4% ± 4.9% 18.4% ± 0.4% 16.5% ± 1.9% G2-M 25.8% ± 2.2% 22.8% ± 1.1% 14.5% ± 0.2% * 21.5% ± 4.6% Sub G1 0.4% ±0.1% 0.5% ± 0.2% 0.2% ± 0.1% 0.2% ± 0.4% * p<0.05 compared to ControlFig.2 The effect of Chinese medicine ingredients on the cell cycle distribution in prostate cancer cell lines PC-3 - 3/82018101446 21 Nov 20181. VersicolorLNCaP2. Astragallus45000 η40000 35000 30000 25000 20000 - y T T T |15000 -3. Ligustrum
- 4. Mix40000 45000 Ί35000 30000 25000 20000 15000 10000 5000 0ControlBaseline0.1250.250.5 * p<0.05 compared to ControlFig3. The effect of Chinese medicine ingredients on DNA content in prostate cancer cell lines LNCaP4/82018101446 21 Nov 2018LNCaP
Gq/G, 65.8% ± 1.3% 78.27% ± 2.8% 80.00% ± 1.7% * 79.50% ± 1.9% s 20.4% ± 1.0% 7.12% ± 2.7% 6.95% ± 0.4% * 7.55% ± 0.6% g2-m 14.1% ± 0.8% 14.30% ± 0.1% 12.70% ± 1.6% 12.73% ± 1.7% Sub G1 0.0% ± 0.3% 0.32% ± 0.0% 0.35% ± 0.1% 0.23% ± 0.2% * p<0.05 compared to ControlFig4. The effect of Chinese medicine ingredients on the cell cycle distribution in prostate cancer cell lines LNCaP2018101446 21 Nov 20181. Versicolor - 5/82. Lung cancerA54950000 -|30000 2. Astragalus45000 40000 35000 25000 20000 15000 10000 5000 0ControlBaseline0.1250.250.53. Ligustrum4. Mix * p<0.05 compared to ControlFig. 5 The effect of Chinese medicine ingredients on DNA content in lung cancer cell lines A549
- 6/82018101446 21 Nov 2018A549Mixture
Go/G, S 57.13% ± 1.8% 75.13% ± 1.7%* 11.23% ± 0.8%* 78.65% ± 4.5%* 82.83% ± 3.5% 9.99% ± 4.5% 25.03% ± 4.0% 7.70% ± 2.1%* g2-m 17.87% ± 1.8% 12.63% ± 0.2% 13.07% ± 4.3% 6.90% ± 0.7% Sub G1 0.03% ± 0.4% 1.00% ± 0.7% 0.93% ± 1.5% 0.28% ± 1.8% * p<0.05 compared to ControlFig.6 The effect of Chinese medicine ingredients on the cell cycle distribution in lung cancer cell lines A5492018101446 21 Nov 2018 - 7/8H19751. Versicolor3. Ligustrum * p<0.05 compared to ControlFig. 7 The effect of Chinese medicine ingredients on DNA content in lung cancer cell lines H19752018101446 21 Nov 2018
- 8/8Η1975Go/G1 62.50% ± 2.6%S 16.90% ± 3.0%G2-M 20.33% ± 5.8%Sub G1 0.27% ± 0.2%
78.12% + 0.9% * 81.07% + 3.3% * 82.57% + 2.6% 12.93% + 0.8% 7.52% + 1.9% * 8.01% + 2.2% 8.54% + 1.2% * 10.91% + 1.3% * 9.04% + 0.9% 0.41% + 1.5% 0.50% + 0.3% 0.38% + 0.1% * p<0.05 compared to ControlFig.8 The effect of Chinese medicine ingredients on the cell cycle in lung cancer cell lines H1975
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| AU2018101446A AU2018101446A4 (en) | 2018-09-27 | 2018-09-27 | A preparation of anti-cancer fungi combined with traditional Chinese medicine and it's preparation method |
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| AU2018101446A AU2018101446A4 (en) | 2018-09-27 | 2018-09-27 | A preparation of anti-cancer fungi combined with traditional Chinese medicine and it's preparation method |
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| AU2018101446A4 true AU2018101446A4 (en) | 2019-01-03 |
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| AU2018101446A Ceased AU2018101446A4 (en) | 2018-09-27 | 2018-09-27 | A preparation of anti-cancer fungi combined with traditional Chinese medicine and it's preparation method |
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- 2018-09-27 AU AU2018101446A patent/AU2018101446A4/en not_active Ceased
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