AU2017258523B2 - Method of Nucleic Acid sequence determination - Google Patents

Method of Nucleic Acid sequence determination Download PDF

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Publication number
AU2017258523B2
AU2017258523B2 AU2017258523A AU2017258523A AU2017258523B2 AU 2017258523 B2 AU2017258523 B2 AU 2017258523B2 AU 2017258523 A AU2017258523 A AU 2017258523A AU 2017258523 A AU2017258523 A AU 2017258523A AU 2017258523 B2 AU2017258523 B2 AU 2017258523B2
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AU
Australia
Prior art keywords
nucleotide
nucleic acid
polymerase
dna polymerase
optionally
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Ceased
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AU2017258523A
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English (en)
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AU2017258523A1 (en
Inventor
Pinar IYIDOGAN
Kandaswamy Vijayan
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Pacific Biosciences of California Inc
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Pacific Biosciences of California Inc
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Assigned to PACIFIC BIOSCIENCES OF CALIFORNIA, INC. reassignment PACIFIC BIOSCIENCES OF CALIFORNIA, INC. Request for Assignment Assignors: Omniome, Inc.
Ceased legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/518—Detection characterised by immobilisation to a surface characterised by the immobilisation of the nucleic acid sample or target
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2017258523A 2016-04-29 2017-04-28 Method of Nucleic Acid sequence determination Ceased AU2017258523B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201662329489P 2016-04-29 2016-04-29
US62/329,489 2016-04-29
US201762444733P 2017-01-10 2017-01-10
US62/444,733 2017-01-10
PCT/US2017/030135 WO2017190012A1 (en) 2016-04-29 2017-04-28 Method of nucleic acid sequence determination

Publications (2)

Publication Number Publication Date
AU2017258523A1 AU2017258523A1 (en) 2018-11-22
AU2017258523B2 true AU2017258523B2 (en) 2020-08-13

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
AU2017258523A Ceased AU2017258523B2 (en) 2016-04-29 2017-04-28 Method of Nucleic Acid sequence determination

Country Status (9)

Country Link
US (2) US10584379B2 (https=)
EP (1) EP3449011B1 (https=)
JP (1) JP6915939B2 (https=)
KR (1) KR102231977B1 (https=)
CN (1) CN109154024B (https=)
AU (1) AU2017258523B2 (https=)
CA (1) CA3022431C (https=)
SG (1) SG11201809522WA (https=)
WO (1) WO2017190012A1 (https=)

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US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
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WO2019161381A1 (en) 2018-02-16 2019-08-22 Illumina, Inc. Charge-tagged nucleotides and methods of use thereof
EP4234718A3 (en) 2018-04-26 2023-11-29 Pacific Biosciences Of California, Inc. Methods and compositions for stabilizing nucleic acid-nucleotide-polymerase complexes
AU2019269615B2 (en) 2018-05-17 2023-04-06 Recognition AnalytiX, Inc. Device, system and method for direct electrical measurement of enzyme activity
JP2021525078A (ja) 2018-05-31 2021-09-24 オムニオム インコーポレイテッドＯｍｎｉｏｍｅ， Ｉｎｃ． 核酸配列決定におけるシグナル対ノイズの増加
WO2020023362A1 (en) 2018-07-24 2020-01-30 Omniome, Inc. Serial formation of ternary complex species
EP3853358A1 (en) 2018-09-17 2021-07-28 Omniome, Inc. Engineered polymerases for improved sequencing
US10768173B1 (en) 2019-09-06 2020-09-08 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
US10876148B2 (en) 2018-11-14 2020-12-29 Element Biosciences, Inc. De novo surface preparation and uses thereof
US10704094B1 (en) 2018-11-14 2020-07-07 Element Biosciences, Inc. Multipart reagents having increased avidity for polymerase binding
WO2020101795A1 (en) 2018-11-15 2020-05-22 Omniome, Inc. Electronic detection of nucleic acid structure
EP3891304A1 (en) 2018-12-04 2021-10-13 Omniome, Inc. Mixed-phase fluids for nucleic acid sequencing and other analytical assays
EP3899032A2 (en) 2018-12-20 2021-10-27 Omniome, Inc. Temperature control for analysis of nucleic acids and other analytes
US12351855B2 (en) 2019-01-30 2025-07-08 Arizona Board Of Regents On Behalf Of Arizona State University Bioelectronic circuits, systems and methods for preparing and using them
WO2020167574A1 (en) 2019-02-14 2020-08-20 Omniome, Inc. Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes
US12313627B2 (en) 2019-05-01 2025-05-27 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
WO2020242901A1 (en) * 2019-05-24 2020-12-03 Element Biosciences, Inc. Polymerase-nucleotide conjugates for sequencing by trapping
US12391929B2 (en) 2019-05-24 2025-08-19 Element Biosciences, Inc. Polymerase-nucleotide conjugates for sequencing by trapping
CA3140279A1 (en) * 2019-06-20 2020-12-24 Arizona Board Of Regents On Behalf Of Arizona State University Direct electrical readout of nucleic acid sequences
CN114450401A (zh) * 2019-06-21 2022-05-06 赛默飞世尔科技波罗的海封闭股份公司 可用于对用于制备下一代测序文库的核酸进行标记的拴系有寡聚核苷酸的三磷酸核苷酸
CN114728996B (zh) 2019-09-10 2022-11-29 加利福尼亚太平洋生物科学股份有限公司 核苷酸的可逆修饰
US11287422B2 (en) 2019-09-23 2022-03-29 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
EP4045683B1 (en) 2019-10-18 2025-02-19 Pacific Biosciences of California, Inc. Methods for capping nucleic acids
EP4055155A1 (en) 2019-11-08 2022-09-14 Pacific Biosciences of California, Inc. Engineered polymerases for improved sequencing by binding
DE202019106694U1 (de) 2019-12-02 2020-03-19 Omniome, Inc. System zur Sequenzierung von Nukleinsäuren in Fluidschaum
DE202019106695U1 (de) 2019-12-02 2020-03-19 Omniome, Inc. System zur Sequenzierung von Nukleinsäuren in Fluidschaum
CA3173798A1 (en) 2020-03-03 2021-09-10 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing double stranded nucleic acids
IL297732A (en) 2020-04-30 2022-12-01 Univ Arizona State Methods for sequencing biopolymers
US20230183798A1 (en) 2020-05-05 2023-06-15 Pacific Biosciences Of California, Inc. Compositions and methods for modifying polymerase-nucleic acid complexes
US20210403993A1 (en) * 2020-06-30 2021-12-30 Illumina, Inc. Catalytically controlled sequencing by synthesis to produce scarless dna
US20220356519A1 (en) 2021-05-10 2022-11-10 Pacific Biosciences Of California, Inc. Single-molecule seeding and amplification on a surface
WO2022240766A1 (en) 2021-05-10 2022-11-17 Pacific Biosciences Of California, Inc. Dna amplification buffer replenishment during rolling circle amplification
AU2022316142A1 (en) 2021-07-21 2024-02-22 Element Biosciences, Inc. Optical systems for nucleic acid sequencing and methods thereof
CN115917006A (zh) * 2021-09-07 2023-04-04 深圳华大智造科技股份有限公司 一种用于分析靶多核苷酸的序列的方法
WO2023081485A1 (en) 2021-11-08 2023-05-11 Pacific Biosciences Of California, Inc. Stepwise sequencing of a polynucleotide with a homogenous reaction mixture
EP4573213A1 (en) 2022-08-15 2025-06-25 Element Biosciences, Inc. Spatially resolved surface capture of nucleic acids
WO2025010160A1 (en) 2023-07-06 2025-01-09 Pacific Biosciences Of California, Inc. Methods and compositions for stabilizing concatemers
WO2025244989A1 (en) 2024-05-21 2025-11-27 Pacific Biosciences Of California, Inc. Selectively functionalized nanoscale wells

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Also Published As

Publication number Publication date
EP3449011A1 (en) 2019-03-06
EP3449011B1 (en) 2020-11-04
CN109154024A (zh) 2019-01-04
JP6915939B2 (ja) 2021-08-11
SG11201809522WA (en) 2018-11-29
US10584379B2 (en) 2020-03-10
US20170314072A1 (en) 2017-11-02
CA3022431A1 (en) 2017-11-02
KR102231977B1 (ko) 2021-03-24
KR20190003660A (ko) 2019-01-09
CN109154024B (zh) 2022-03-04
US11976323B2 (en) 2024-05-07
US20200199668A1 (en) 2020-06-25
AU2017258523A1 (en) 2018-11-22
CA3022431C (en) 2023-03-21
WO2017190012A1 (en) 2017-11-02
JP2019526225A (ja) 2019-09-19

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FGA Letters patent sealed or granted (standard patent)
PC Assignment registered

Owner name: PACIFIC BIOSCIENCES OF CALIFORNIA, INC.

Free format text: FORMER OWNER(S): OMNIOME, INC.

MK14 Patent ceased section 143(a) (annual fees not paid) or expired