AU2017215287A1 - Methods of producing viruses - Google Patents
Methods of producing viruses Download PDFInfo
- Publication number
- AU2017215287A1 AU2017215287A1 AU2017215287A AU2017215287A AU2017215287A1 AU 2017215287 A1 AU2017215287 A1 AU 2017215287A1 AU 2017215287 A AU2017215287 A AU 2017215287A AU 2017215287 A AU2017215287 A AU 2017215287A AU 2017215287 A1 AU2017215287 A1 AU 2017215287A1
- Authority
- AU
- Australia
- Prior art keywords
- virus
- cell culture
- cell
- japanese eel
- nodavirus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 241000700605 Viruses Species 0.000 title claims abstract description 293
- 238000000034 method Methods 0.000 title claims abstract description 48
- 238000004113 cell culture Methods 0.000 claims abstract description 138
- 241000252087 Anguilla japonica Species 0.000 claims abstract description 52
- 241000710921 Infectious pancreatic necrosis virus Species 0.000 claims abstract description 50
- 241001660014 Salmon pancreas disease virus Species 0.000 claims abstract description 48
- 235000019688 fish Nutrition 0.000 claims abstract description 42
- 241000251468 Actinopterygii Species 0.000 claims abstract description 40
- 229960005486 vaccine Drugs 0.000 claims abstract description 12
- 230000001902 propagating effect Effects 0.000 claims abstract description 8
- 210000004027 cell Anatomy 0.000 claims description 97
- 241000252084 Anguilla Species 0.000 claims description 46
- 230000003612 virological effect Effects 0.000 claims description 20
- 210000002950 fibroblast Anatomy 0.000 claims description 15
- 210000002919 epithelial cell Anatomy 0.000 claims description 14
- 230000000120 cytopathologic effect Effects 0.000 claims description 11
- 238000003306 harvesting Methods 0.000 claims description 10
- 208000015181 infectious disease Diseases 0.000 claims description 10
- 238000012258 culturing Methods 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 9
- 238000004519 manufacturing process Methods 0.000 claims description 8
- 201000010099 disease Diseases 0.000 claims description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 7
- 239000006228 supernatant Substances 0.000 claims description 6
- 239000001963 growth medium Substances 0.000 claims description 4
- 241000972773 Aulopiformes Species 0.000 claims description 2
- 230000002163 immunogen Effects 0.000 claims description 2
- 238000011321 prophylaxis Methods 0.000 claims description 2
- 235000019515 salmon Nutrition 0.000 claims description 2
- 230000002463 transducing effect Effects 0.000 claims description 2
- 238000011282 treatment Methods 0.000 claims description 2
- 208000024691 pancreas disease Diseases 0.000 claims 1
- 241001493065 dsRNA viruses Species 0.000 description 35
- 241000439574 Decapod penstyldensovirus 1 Species 0.000 description 18
- 241000711804 Infectious hematopoietic necrosis virus Species 0.000 description 18
- 241000546112 Infectious salmon anemia virus Species 0.000 description 18
- 241000993404 Penaeus merguiensis densovirus Species 0.000 description 18
- 241001265687 Taura syndrome virus Species 0.000 description 18
- 241000711825 Viral hemorrhagic septicemia virus Species 0.000 description 18
- 241000965621 Brevidensovirus Species 0.000 description 16
- 241000712464 Orthomyxoviridae Species 0.000 description 15
- 241000709664 Picornaviridae Species 0.000 description 15
- 239000012909 foetal bovine serum Substances 0.000 description 15
- 241000702628 Birnaviridae Species 0.000 description 14
- 241000615461 Dicistroviridae Species 0.000 description 14
- 241000701377 Iridoviridae Species 0.000 description 13
- 241001484257 Nimaviridae Species 0.000 description 13
- 241000723741 Nodaviridae Species 0.000 description 13
- 241000701945 Parvoviridae Species 0.000 description 13
- 241000711931 Rhabdoviridae Species 0.000 description 13
- 230000000644 propagated effect Effects 0.000 description 13
- 241000702247 Reoviridae Species 0.000 description 12
- 241000710924 Togaviridae Species 0.000 description 12
- 108020004414 DNA Proteins 0.000 description 10
- 239000002609 medium Substances 0.000 description 10
- 230000017074 necrotic cell death Effects 0.000 description 10
- 241001279887 Betanodavirus Species 0.000 description 9
- 241000238557 Decapoda Species 0.000 description 9
- 208000016222 Pancreatic disease Diseases 0.000 description 9
- 241000277331 Salmonidae Species 0.000 description 9
- 241001279853 Alphanodavirus Species 0.000 description 8
- 241000710929 Alphavirus Species 0.000 description 8
- 241001324067 Aparavirus Species 0.000 description 8
- 241001533425 Aquabirnavirus Species 0.000 description 8
- 241001533426 Avibirnavirus Species 0.000 description 8
- 241000868841 Blosnavirus Species 0.000 description 8
- 241001533423 Entomobirnavirus Species 0.000 description 8
- 241001661732 Isavirus Species 0.000 description 8
- 241001058063 Whispovirus Species 0.000 description 8
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 7
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 7
- 241001112535 Novirhabdovirus Species 0.000 description 7
- 241000710801 Rubivirus Species 0.000 description 7
- 241001465754 Metazoa Species 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 5
- 229930182566 Gentamicin Natural products 0.000 description 5
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 5
- 229930182816 L-glutamine Natural products 0.000 description 5
- 229960002518 gentamicin Drugs 0.000 description 5
- 238000003752 polymerase chain reaction Methods 0.000 description 5
- 241000197200 Gallinago media Species 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 235000013601 eggs Nutrition 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 238000012163 sequencing technique Methods 0.000 description 4
- 208000009663 Acute Necrotizing Pancreatitis Diseases 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- 206010058096 Pancreatic necrosis Diseases 0.000 description 3
- 229940030156 cell vaccine Drugs 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 230000002458 infectious effect Effects 0.000 description 3
- 241000894007 species Species 0.000 description 3
- HJCMDXDYPOUFDY-WHFBIAKZSA-N Ala-Gln Chemical compound C[C@H](N)C(=O)N[C@H](C(O)=O)CCC(N)=O HJCMDXDYPOUFDY-WHFBIAKZSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
- 208000036142 Viral infection Diseases 0.000 description 2
- 238000000246 agarose gel electrophoresis Methods 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000003111 delayed effect Effects 0.000 description 2
- 238000002337 electrophoretic mobility shift assay Methods 0.000 description 2
- 230000004660 morphological change Effects 0.000 description 2
- 238000010899 nucleation Methods 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 238000005215 recombination Methods 0.000 description 2
- 230000006798 recombination Effects 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000003757 reverse transcription PCR Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 230000009385 viral infection Effects 0.000 description 2
- 241000473391 Archosargus rhomboidalis Species 0.000 description 1
- 101150087323 COI gene Proteins 0.000 description 1
- 201000009030 Carcinoma Diseases 0.000 description 1
- 241000907195 Channa striata Species 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 108091035707 Consensus sequence Proteins 0.000 description 1
- 102000000634 Cytochrome c oxidase subunit IV Human genes 0.000 description 1
- 108050008072 Cytochrome c oxidase subunit IV Proteins 0.000 description 1
- 108010016626 Dipeptides Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000357444 Epinephelus coioides Species 0.000 description 1
- 238000012413 Fluorescence activated cell sorting analysis Methods 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 241000721654 Lepomis macrochirus Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- 241000277275 Oncorhynchus mykiss Species 0.000 description 1
- 241001280377 Oncorhynchus tshawytscha Species 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 238000010222 PCR analysis Methods 0.000 description 1
- 244000184734 Pyrus japonica Species 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 241001417495 Serranidae Species 0.000 description 1
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 229960002648 alanylglutamine Drugs 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 208000010932 epithelial neoplasm Diseases 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 210000002149 gonad Anatomy 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 229940031551 inactivated vaccine Drugs 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000001524 infective effect Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000001320 lysogenic effect Effects 0.000 description 1
- 230000002101 lytic effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000002438 mitochondrial effect Effects 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- 230000029812 viral genome replication Effects 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
- C12N2720/00011—Details
- C12N2720/10011—Birnaviridae
- C12N2720/10034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
- C12N2720/00011—Details
- C12N2720/10011—Birnaviridae
- C12N2720/10051—Methods of production or purification of viral material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
- C12N2720/00011—Details
- C12N2720/10011—Birnaviridae
- C12N2720/10061—Methods of inactivation or attenuation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/30011—Nodaviridae
- C12N2770/30034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/30011—Nodaviridae
- C12N2770/30051—Methods of production or purification of viral material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2796/00—Viruses not covered by groups C12N2710/00 - C12N2795/00
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1601861.6 | 2016-02-02 | ||
| GBGB1601861.6A GB201601861D0 (en) | 2016-02-02 | 2016-02-02 | Methods of producing viruses |
| GB201618549 | 2016-11-03 | ||
| GB1618549.8 | 2016-11-03 | ||
| PCT/GB2017/050259 WO2017134441A1 (en) | 2016-02-02 | 2017-02-02 | Methods of producing viruses |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2017215287A1 true AU2017215287A1 (en) | 2018-08-16 |
Family
ID=58018136
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2017215287A Abandoned AU2017215287A1 (en) | 2016-02-02 | 2017-02-02 | Methods of producing viruses |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US10450544B2 (https=) |
| EP (1) | EP3411064B1 (https=) |
| JP (1) | JP2019511208A (https=) |
| CN (1) | CN109414494A (https=) |
| AU (1) | AU2017215287A1 (https=) |
| BR (1) | BR112018015527A2 (https=) |
| CA (1) | CA3011920A1 (https=) |
| CL (1) | CL2018002042A1 (https=) |
| ES (1) | ES2795040T3 (https=) |
| SG (1) | SG11201806182RA (https=) |
| WO (1) | WO2017134441A1 (https=) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111118171A (zh) * | 2019-12-31 | 2020-05-08 | 广东省测试分析研究所（中国广州分析测试中心） | 一种鉴别鱼翅种的dna条形码引物对、试剂盒及方法 |
| CN113122510A (zh) * | 2021-04-12 | 2021-07-16 | 中国水产科学研究院黑龙江水产研究所 | 一种传染性造血器官坏死病疫苗及其病毒在胖头鱥肌肉细胞上扩增的方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5780448A (en) * | 1995-11-07 | 1998-07-14 | Ottawa Civic Hospital Loeb Research | DNA-based vaccination of fish |
| TWI220904B (en) * | 1998-12-03 | 2004-09-11 | Nat Science Council | An immortal cell line derived from grouper Epinephelus coioides and its applications therein |
| TWI347364B (en) | 2003-02-19 | 2011-08-21 | Novartis Ag | Inactivated nodavirus vaccine |
| NO332608B2 (no) * | 2011-03-16 | 2012-11-19 | Fish Vet Group Fvg | Levende avirulent IPNV og vaksine for profylakse eller behandling av IPN-sykdom hos fisk |
| CN104531608A (zh) * | 2015-01-12 | 2015-04-22 | 福建省农业科学院生物技术研究所 | 一种欧洲鳗鲡肝脏细胞系及其构建方法和应用 |
| CN105192298A (zh) * | 2015-11-11 | 2015-12-30 | 浙江锦天生物科技有限公司 | 一种日本鳗鲡用复合微生态饲料添加剂 |
-
2017
- 2017-02-02 WO PCT/GB2017/050259 patent/WO2017134441A1/en not_active Ceased
- 2017-02-02 US US16/074,501 patent/US10450544B2/en not_active Expired - Fee Related
- 2017-02-02 EP EP17704811.3A patent/EP3411064B1/en active Active
- 2017-02-02 SG SG11201806182RA patent/SG11201806182RA/en unknown
- 2017-02-02 AU AU2017215287A patent/AU2017215287A1/en not_active Abandoned
- 2017-02-02 ES ES17704811T patent/ES2795040T3/es active Active
- 2017-02-02 CN CN201780009494.1A patent/CN109414494A/zh active Pending
- 2017-02-02 BR BR112018015527A patent/BR112018015527A2/pt not_active IP Right Cessation
- 2017-02-02 CA CA3011920A patent/CA3011920A1/en not_active Abandoned
- 2017-02-02 JP JP2018541216A patent/JP2019511208A/ja not_active Abandoned
-
2018
- 2018-07-30 CL CL2018002042A patent/CL2018002042A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US10450544B2 (en) | 2019-10-22 |
| EP3411064B1 (en) | 2020-02-26 |
| ES2795040T3 (es) | 2020-11-20 |
| WO2017134441A1 (en) | 2017-08-10 |
| SG11201806182RA (en) | 2018-08-30 |
| BR112018015527A2 (pt) | 2018-12-26 |
| CL2018002042A1 (es) | 2019-03-22 |
| EP3411064A1 (en) | 2018-12-12 |
| CN109414494A (zh) | 2019-03-01 |
| CA3011920A1 (en) | 2017-08-10 |
| US20190040354A1 (en) | 2019-02-07 |
| JP2019511208A (ja) | 2019-04-25 |
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