AU2016282350A1 - Targeted enrichment of long nucleotide sequences using microfluidic partitioning - Google Patents

Targeted enrichment of long nucleotide sequences using microfluidic partitioning Download PDF

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Publication number
AU2016282350A1
AU2016282350A1 AU2016282350A AU2016282350A AU2016282350A1 AU 2016282350 A1 AU2016282350 A1 AU 2016282350A1 AU 2016282350 A AU2016282350 A AU 2016282350A AU 2016282350 A AU2016282350 A AU 2016282350A AU 2016282350 A1 AU2016282350 A1 AU 2016282350A1
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AU
Australia
Prior art keywords
dna
droplets
primer
target
target dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
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AU2016282350A
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English (en)
Inventor
Thomas Kvist
Esben Bjørn MADSEN
Marie Just Mikkelsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Samplix SARL
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Samplix SARL
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Publication date
Application filed by Samplix SARL filed Critical Samplix SARL
Publication of AU2016282350A1 publication Critical patent/AU2016282350A1/en
Abandoned legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Dispersion Chemistry (AREA)
AU2016282350A 2015-06-26 2016-06-24 Targeted enrichment of long nucleotide sequences using microfluidic partitioning Abandoned AU2016282350A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201562185111P 2015-06-26 2015-06-26
US62/185,111 2015-06-26
DKPA201570449 2015-07-07
DKPA201570449 2015-07-07
PCT/EP2016/064719 WO2016207379A1 (en) 2015-06-26 2016-06-24 Targeted enrichment of long nucleotide sequences using microfluidic partitioning

Publications (1)

Publication Number Publication Date
AU2016282350A1 true AU2016282350A1 (en) 2018-01-18

Family

ID=57584736

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2016282350A Abandoned AU2016282350A1 (en) 2015-06-26 2016-06-24 Targeted enrichment of long nucleotide sequences using microfluidic partitioning

Country Status (6)

Country Link
US (2) US10738354B2 (https=)
EP (2) EP3770271B1 (https=)
JP (1) JP6936155B2 (https=)
AU (1) AU2016282350A1 (https=)
DK (1) DK3770271T3 (https=)
WO (1) WO2016207379A1 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2015273480A1 (en) * 2014-06-11 2016-12-08 Samplix S.A.R.L. Nucleotide sequence exclusion enrichment by droplet sorting (needls)
WO2018221513A1 (ja) * 2017-05-31 2018-12-06 キヤノン株式会社 油性組成物、およびそれを用いた分析方法
DK182109B1 (en) 2019-08-02 2025-08-18 Samplix Aps System and method for amplifying nucleic acids from single cells
WO2021146168A1 (en) * 2020-01-13 2021-07-22 Fluent Biosciences Inc. Methods and systems for amplifying low concentrations of nucleic acids
DK181994B1 (en) * 2021-09-28 2025-05-15 Samplix Aps Instrument for sorting of particles
EP4255632A1 (en) 2020-12-01 2023-10-11 Samplix ApS System and method for sorting of particles
DK181862B1 (en) * 2022-03-21 2025-02-24 Samplix Aps Method and kit for targeted enrichment of large dna molecules for long-read sequencing using facs or microfluidic partitioning

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2977455B1 (en) 2009-06-15 2020-04-15 Complete Genomics, Inc. Method for long fragment read sequencing
US20120164633A1 (en) 2010-12-27 2012-06-28 Ibis Biosciences, Inc. Digital droplet sequencing
AU2013366478A1 (en) 2012-12-21 2015-07-09 Samplix S.A.R.L. Probability-directed isolation of nucleotide sequences (pins)
US20140303005A1 (en) 2013-04-05 2014-10-09 Raindance Technologies, Inc. Rare cell analysis after negative selection
WO2014172373A2 (en) 2013-04-19 2014-10-23 Raindance Technologies, Inc Digital analyte analysis
AU2015273480A1 (en) * 2014-06-11 2016-12-08 Samplix S.A.R.L. Nucleotide sequence exclusion enrichment by droplet sorting (needls)
US10697007B2 (en) * 2014-06-27 2020-06-30 The Regents Of The University Of California PCR-activated sorting (PAS)

Also Published As

Publication number Publication date
EP3770271A1 (en) 2021-01-27
DK3770271T3 (da) 2023-05-08
US20200332353A1 (en) 2020-10-22
EP3770271B1 (en) 2023-02-08
JP6936155B2 (ja) 2021-09-15
WO2016207379A1 (en) 2016-12-29
JP2018518968A (ja) 2018-07-19
US20180179589A1 (en) 2018-06-28
CA2990092A1 (en) 2016-12-29
US11572584B2 (en) 2023-02-07
EP3314012B1 (en) 2020-09-09
EP3314012A1 (en) 2018-05-02
US10738354B2 (en) 2020-08-11

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Legal Events

Date Code Title Description
MK4 Application lapsed section 142(2)(d) - no continuation fee paid for the application