AU2012225123B2 - Method and system for portable cell detection and analysis using microfluidic technology - Google Patents

Method and system for portable cell detection and analysis using microfluidic technology Download PDF

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Publication number
AU2012225123B2
AU2012225123B2 AU2012225123A AU2012225123A AU2012225123B2 AU 2012225123 B2 AU2012225123 B2 AU 2012225123B2 AU 2012225123 A AU2012225123 A AU 2012225123A AU 2012225123 A AU2012225123 A AU 2012225123A AU 2012225123 B2 AU2012225123 B2 AU 2012225123B2
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present
fluidic chip
analysis
detection
images
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AU2012225123A
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AU2012225123A1 (en
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James Stewart Aitchison
Lu Chen
James Jiahua DOU
Rakesh Kumar NAYYAR
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University of Toronto
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University of Toronto
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Assigned to THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO reassignment THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO Request for Assignment Assignors: AITCHISON, JAMES, CHEN, LU, DOU, JAMES
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles or throttle valves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
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    • G—PHYSICS
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
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    • G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
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    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
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    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
    • G—PHYSICS
    • G01—MEASURING; TESTING
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    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/02—Mechanical
    • G01N2201/022—Casings
    • G01N2201/0221—Portable; cableless; compact; hand-held

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Dispersion Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Signal Processing (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Optical Measuring Cells (AREA)
AU2012225123A 2011-03-07 2012-03-07 Method and system for portable cell detection and analysis using microfluidic technology Ceased AU2012225123B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161449840P 2011-03-07 2011-03-07
US61/449,840 2011-03-07
PCT/CA2012/000227 WO2012119243A2 (en) 2011-03-07 2012-03-07 Method and system for cell detection and analysis

Publications (2)

Publication Number Publication Date
AU2012225123A1 AU2012225123A1 (en) 2013-09-26
AU2012225123B2 true AU2012225123B2 (en) 2016-07-07

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AU2012225123A Ceased AU2012225123B2 (en) 2011-03-07 2012-03-07 Method and system for portable cell detection and analysis using microfluidic technology

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US (1) US20140170679A1 (enExample)
EP (1) EP2683810A4 (enExample)
KR (4) KR102307064B1 (enExample)
CN (2) CN103562373A (enExample)
AU (1) AU2012225123B2 (enExample)
BR (1) BR112013022995A8 (enExample)
CA (1) CA2828487C (enExample)
SG (1) SG193009A1 (enExample)
WO (1) WO2012119243A2 (enExample)
ZA (1) ZA201306705B (enExample)

Families Citing this family (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9513224B2 (en) 2013-02-18 2016-12-06 Theranos, Inc. Image analysis and measurement of biological samples
EP2877834B1 (en) 2012-07-25 2018-09-05 Theranos, Inc. System for analysing a sample
MX362545B (es) * 2013-02-18 2019-01-24 Theranos Ip Co Llc Analisis de imagen y medicion de muestras biologicas.
HK1226480A1 (zh) * 2013-12-12 2017-09-29 梅斯医疗电子系统有限公司 家庭测试设备
CN103954546A (zh) * 2014-04-30 2014-07-30 西安理工大学 基于光探测器与微流控技术的流式细胞仪
CN104212705B (zh) * 2014-09-19 2016-02-10 成都劲宏科技有限公司 一种基于脉冲检测技术的双通道细胞微流图像采集系统
KR20170066498A (ko) * 2014-09-29 2017-06-14 칩케어 코포레이션 카트리지 및 유체 칩을 포함하는 세포의 광학적 검출을 위한 장치 및 그 방법
WO2016083898A1 (en) * 2014-11-28 2016-06-02 Chipcare Corporation Multiplex bead array assay
CN104486549B (zh) * 2014-12-29 2017-07-25 中国科学院长春光学精密机械与物理研究所 一种用于成像流式细胞仪的高通量拍摄方法
WO2016122560A1 (en) 2015-01-30 2016-08-04 Hewlett-Packard Development Company, L.P. Vented microfluidic reservoirs
CN106153879A (zh) * 2015-04-08 2016-11-23 李木 用活性微小生物实现疾病诊断的微流控系统及其诊断方法
US10379049B2 (en) * 2015-10-15 2019-08-13 Lumos Diagnostics IP Pty Ltd Device for reading an IVD assay
US11698332B2 (en) 2015-11-24 2023-07-11 Hewlett-Packard Development Company, L.P. Devices having a sample delivery component
CN108139241B (zh) 2016-01-22 2020-02-14 惠普发展公司，有限责任合伙企业 具有对感测区中的颗粒聚集的控制的流体感测
CN115487880A (zh) 2016-03-24 2022-12-20 生物动力学公司 一次性射流卡盘及组件
EP3479102A1 (en) * 2016-07-04 2019-05-08 CellTool GmbH Device and method for the determination of transfection
US10768105B1 (en) 2016-07-29 2020-09-08 Labrador Diagnostics Llc Image analysis and measurement of biological samples
US12009078B2 (en) 2016-10-17 2024-06-11 Reliant Immune Diagnostics, Inc. System and method for medical escalation and intervention that is a direct result of a remote diagnostic test
US9857372B1 (en) 2016-10-17 2018-01-02 Reliant Immune Diagnostics, LLC Arbovirus indicative birth defect risk test
US11579145B2 (en) 2016-10-17 2023-02-14 Reliant Immune Diagnostics, Inc. System and method for image analysis of medical test results
US11651866B2 (en) 2016-10-17 2023-05-16 Reliant Immune Diagnostics, Inc. System and method for real-time insurance quote in response to a self-diagnostic test
US11802868B2 (en) 2016-10-17 2023-10-31 Reliant Immune Diagnostics, Inc. System and method for variable function mobile application for providing medical test results
US10902951B2 (en) 2016-10-17 2021-01-26 Reliant Immune Diagnostics, Inc. System and method for machine learning application for providing medical test results using visual indicia
US11107585B2 (en) 2016-10-17 2021-08-31 Reliant Immune Diagnostics, Inc System and method for a digital consumer medical wallet and storehouse
US11693002B2 (en) 2016-10-17 2023-07-04 Reliant Immune Diagnostics, Inc. System and method for variable function mobile application for providing medical test results using visual indicia to determine medical test function type
TWI614500B (zh) * 2016-11-21 2018-02-11 國立清華大學 細胞檢測晶片的影像定位與拼接方法及影像檢測系統
US11170877B2 (en) 2016-12-14 2021-11-09 Reliant Immune Diagnostics, LLC System and method for correlating retail testing product to medical diagnostic code
US10527555B2 (en) 2016-12-14 2020-01-07 Reliant Immune Disgnostics, Inc. System and method for visual trigger to perform diagnostic test
US11164680B2 (en) 2016-12-14 2021-11-02 Reliant Immune Diagnostics, Inc. System and method for initiating telemedicine conference using self-diagnostic test
US11295859B2 (en) 2016-12-14 2022-04-05 Reliant Immune Diagnostics, Inc. System and method for handing diagnostic test results to telemedicine provider
WO2018112251A1 (en) 2016-12-14 2018-06-21 Reliant Immune Diagnostics, LLC Two-sided flow-through immunoassay
US11915810B2 (en) 2016-12-14 2024-02-27 Reliant Immune Diagnostics, Inc. System and method for transmitting prescription to pharmacy using self-diagnostic test and telemedicine
US11599908B2 (en) 2016-12-14 2023-03-07 Reliant Immune Diagnostics, Inc. System and method for advertising in response to diagnostic test
US11594337B2 (en) 2016-12-14 2023-02-28 Reliant Immune Diagnostics, Inc. System and method for advertising in response to diagnostic test results
US10631031B2 (en) 2016-12-14 2020-04-21 Reliant Immune Diagnostics, Inc. System and method for television network in response to input
JP7095075B2 (ja) * 2017-04-28 2022-07-04 マイクロワイズ アンパーツゼルスカブ 生きた有機体を定量化するための方法および装置、ならびに装置の使用
EP3622084A4 (en) 2017-05-08 2021-02-17 Biological Dynamics, Inc. METHODS AND SYSTEMS FOR PROCESSING ANALYTICAL INFORMATION
CN107101932B (zh) * 2017-05-28 2021-07-27 合肥赫博医疗器械有限责任公司 一种微量全自动血细胞与血红蛋白计量装置的使用方法
EP3697537A4 (en) 2017-10-18 2021-10-20 Group K Diagnostics, Inc. SINGLE-LAYER MICROFLUIDIC DEVICE AND ITS MANUFACTURING AND USE METHODS
US11200986B2 (en) 2017-11-10 2021-12-14 Reliant Immune Diagnostics, Inc. Database and machine learning in response to parallel serial dual microfluidic chip
US11527324B2 (en) 2017-11-10 2022-12-13 Reliant Immune Diagnostics, Inc. Artificial intelligence response system based on testing with parallel/serial dual microfluidic chip
US11437142B2 (en) 2017-11-10 2022-09-06 Reliant Immune Diagnostics, Inc. Biofluidic triggering system and method
US11041185B2 (en) 2017-11-10 2021-06-22 Reliant Immune Diagnostics, Inc. Modular parallel/serial dual microfluidic chip
US11124821B2 (en) 2017-11-10 2021-09-21 Reliant Immune Diagnostics, Inc. Microfluidic testing system with cell capture/analysis regions for processing in a parallel and serial manner
US10930381B2 (en) 2017-11-10 2021-02-23 Reliant Immune Diagnostics, Inc. Microfluidic testing system for mobile veterinary applications
US10930380B2 (en) 2017-11-10 2021-02-23 Reliant Immune Diagnostics, Inc. Communication loop and record loop system for parallel/serial dual microfluidic chip
US11731132B2 (en) 2017-12-19 2023-08-22 Biological Dynamics, Inc. Methods and devices for detection of multiple analytes from a biological sample
WO2019195196A1 (en) 2018-04-02 2019-10-10 Biological Dynamics, Inc. Dielectric materials
CN108318468B (zh) * 2018-05-05 2024-01-16 哈尔滨索飞永诚科技有限公司 一种用于对液体样品中荧光染料染色微粒进行快速计数的计数系统
US10835122B2 (en) * 2018-05-14 2020-11-17 Reliant Immune Diagnostics, Inc. System and method for image processing of medical test results using generalized curve field transform
CA3100701A1 (en) * 2018-05-31 2019-12-05 Berkeley Lights, Inc. Automated detection and characterization of micro-objects in microfluidic devices
CN108732103B (zh) * 2018-06-01 2020-10-30 大连晓辉医药科技有限公司 一种基于光流控成像光谱的细胞检测和分类装置
US11125749B2 (en) 2018-06-06 2021-09-21 Reliant Immune Diagnostics, Inc. System and method for remote colorimetry and ratiometric comparison and quantification in analysis of medical test results
US11232872B2 (en) 2018-06-06 2022-01-25 Reliant Immune Diagnostics, Inc. Code trigger telemedicine session
US10636527B2 (en) 2018-06-06 2020-04-28 Reliant Immune Diagnostics, Inc. System and method for quantifying, ensuring, and triggering the prescriptive authority for a telemedicine session
US11112406B2 (en) 2018-06-15 2021-09-07 Reliant Immune Diagnostics, Inc. System and method for digital remote primary, secondary, and tertiary color calibration via smart device in analysis of medical test results
KR101970690B1 (ko) 2018-09-28 2019-08-13 피엠씨씨 주식회사 다중 형광 신호 측정을 위한 광수신장치
USD879999S1 (en) 2018-11-02 2020-03-31 Group K Diagnostics, Inc. Microfluidic device
CN111912769B (zh) * 2019-05-09 2025-08-05 苏州中科苏净生物技术有限公司 一种生乳体细胞计数仪及其计数方法
CN111398237A (zh) * 2020-05-07 2020-07-10 成都量蚁科技有限公司 细胞检测装置及应用和细胞分类的检测方法
US12469162B2 (en) 2020-08-31 2025-11-11 Element Biosciences, Inc. Primary analysis in next generation sequencing
US12505571B2 (en) 2020-08-31 2025-12-23 Element Biosciences, Inc. Primary analysis in next generation sequencing
US11200446B1 (en) * 2020-08-31 2021-12-14 Element Biosciences, Inc. Single-pass primary analysis
CN112683756A (zh) * 2020-12-01 2021-04-20 山东交通学院 一种具有细胞检测功能的便携式移动设备及其使用方法
WO2022140291A1 (en) * 2020-12-21 2022-06-30 Singular Genomics Systems, Inc. Systems and methods for multicolor imaging
CN113552041B (zh) * 2021-06-08 2022-10-11 上海交通大学 一种基于单颗粒成像的外泌体亚型分析方法
KR20230126592A (ko) 2022-02-23 2023-08-30 주식회사 엘지에너지솔루션 전지 팩
CN115656134B (zh) * 2022-12-23 2023-04-11 深圳市合川医疗科技有限公司 基于微流控芯片的细胞检测方法、设备、存储介质及装置
WO2026131650A1 (en) 2024-12-16 2026-06-25 Xomexbio Method for loading biological particles with a molecule of interest
EP4759410A1 (en) * 2024-12-16 2026-06-17 Somestech Microfluidic and nanofluidic chip device

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070086918A1 (en) * 2005-04-01 2007-04-19 Hartley Lee F Cytometer
US20090081773A1 (en) * 2007-09-25 2009-03-26 Cytyc Corporation Microfluidic apparatus for manipulating imaging and analyzing cells of a cytological specimen

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8172671B2 (en) * 2002-04-19 2012-05-08 Walker Digital, Llc Method and apparatus for facilitating play of a gaming device
US7978329B2 (en) * 2000-08-02 2011-07-12 Honeywell International Inc. Portable scattering and fluorescence cytometer
GB2383127B (en) * 2001-12-12 2004-10-20 Proimmune Ltd Device and method for investigating analytes in liquid suspension or solution
DE60332046D1 (de) * 2002-10-23 2010-05-20 Univ Princeton Verfahren zur kontinuierlichen teilchentrennung unter verwendungvon zu feldern asymmetrisch ausgerichteten hindernisarrays
US20080213821A1 (en) * 2004-05-06 2008-09-04 Nanyang Technological University Microfluidic Cell Sorter System
WO2006014680A1 (en) * 2004-07-19 2006-02-09 Cell Biosciences, Inc. Methods and devices for analyte detection
WO2006071770A2 (en) * 2004-12-23 2006-07-06 I-Stat Corporation Molecular diagnostics system and methods
EP1910824A4 (en) * 2005-05-31 2012-11-21 Labnow Inc METHOD AND COMPOSITIONS RELATED TO THE PREPARATION AND USE OF A WHITE BLOOD IMAGE
US8270781B2 (en) * 2005-10-28 2012-09-18 The Regents Of The University Of California Apparatus and method for improved optical detection of particles in fluid
US7629124B2 (en) * 2006-06-30 2009-12-08 Canon U.S. Life Sciences, Inc. Real-time PCR in micro-channels
TWI324750B (en) * 2006-12-08 2010-05-11 Univ Southern Taiwan Tech Microscopic image analysis method and system of microfluidic cells
EP2247715A1 (en) * 2008-02-01 2010-11-10 The Regents of the University of California Microfluidic imaging cytometry
CA2756045A1 (en) * 2009-03-20 2010-09-23 Biorad Laboratories, Inc. Serial-line-scan-encoded multi-color fluorescence microscopy and imaging flow cytometry
WO2010115167A2 (en) * 2009-04-03 2010-10-07 The Regents Of The University Of California Methods and devices for sorting cells and other biological particulates
WO2010148252A1 (en) 2009-06-17 2010-12-23 Jody Vykoukal Method and apparatus for quantitative microimaging

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070086918A1 (en) * 2005-04-01 2007-04-19 Hartley Lee F Cytometer
US20090081773A1 (en) * 2007-09-25 2009-03-26 Cytyc Corporation Microfluidic apparatus for manipulating imaging and analyzing cells of a cytological specimen

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