AU2010340802B2 - Methods and compositions for altering temperature sensing in eukaryotic organisms - Google Patents
Methods and compositions for altering temperature sensing in eukaryotic organisms Download PDFInfo
- Publication number
- AU2010340802B2 AU2010340802B2 AU2010340802A AU2010340802A AU2010340802B2 AU 2010340802 B2 AU2010340802 B2 AU 2010340802B2 AU 2010340802 A AU2010340802 A AU 2010340802A AU 2010340802 A AU2010340802 A AU 2010340802A AU 2010340802 B2 AU2010340802 B2 AU 2010340802B2
- Authority
- AU
- Australia
- Prior art keywords
- temperature
- nucleosome
- altering
- nucleic acid
- expression
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 238000000034 method Methods 0.000 title claims description 105
- 239000000203 mixture Substances 0.000 title claims description 8
- 102100023919 Histone H2A.Z Human genes 0.000 claims abstract description 291
- 101000905054 Homo sapiens Histone H2A.Z Proteins 0.000 claims abstract description 288
- 101001084710 Drosophila melanogaster Histone H2A.v Proteins 0.000 claims abstract description 287
- 108010047956 Nucleosomes Proteins 0.000 claims abstract description 200
- 210000001623 nucleosome Anatomy 0.000 claims abstract description 200
- 230000004044 response Effects 0.000 claims abstract description 133
- 241000196324 Embryophyta Species 0.000 claims description 122
- 108090000623 proteins and genes Proteins 0.000 claims description 105
- 230000014509 gene expression Effects 0.000 claims description 87
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 claims description 48
- 150000007523 nucleic acids Chemical group 0.000 claims description 45
- 230000021736 acetylation Effects 0.000 claims description 43
- 238000006640 acetylation reaction Methods 0.000 claims description 43
- 230000003247 decreasing effect Effects 0.000 claims description 43
- 230000002401 inhibitory effect Effects 0.000 claims description 35
- 230000001965 increasing effect Effects 0.000 claims description 29
- 230000001105 regulatory effect Effects 0.000 claims description 29
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 26
- 230000000694 effects Effects 0.000 claims description 23
- 102000004190 Enzymes Human genes 0.000 claims description 22
- 108090000790 Enzymes Proteins 0.000 claims description 22
- 230000001419 dependent effect Effects 0.000 claims description 22
- 230000004481 post-translational protein modification Effects 0.000 claims description 21
- 235000018102 proteins Nutrition 0.000 claims description 20
- 102000004169 proteins and genes Human genes 0.000 claims description 20
- 240000007594 Oryza sativa Species 0.000 claims description 16
- 235000007164 Oryza sativa Nutrition 0.000 claims description 16
- 235000009566 rice Nutrition 0.000 claims description 16
- 102000003964 Histone deacetylase Human genes 0.000 claims description 14
- 108090000353 Histone deacetylase Proteins 0.000 claims description 14
- 235000013339 cereals Nutrition 0.000 claims description 12
- 230000012010 growth Effects 0.000 claims description 12
- 125000003588 lysine group Chemical class [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 claims description 12
- 108020004707 nucleic acids Proteins 0.000 claims description 12
- 102000039446 nucleic acids Human genes 0.000 claims description 12
- 230000002829 reductive effect Effects 0.000 claims description 12
- 210000001519 tissue Anatomy 0.000 claims description 11
- 240000008042 Zea mays Species 0.000 claims description 10
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 10
- 235000018977 lysine Nutrition 0.000 claims description 10
- 241000209140 Triticum Species 0.000 claims description 9
- 235000021307 Triticum Nutrition 0.000 claims description 9
- 108020004705 Codon Proteins 0.000 claims description 7
- 238000012228 RNA interference-mediated gene silencing Methods 0.000 claims description 7
- 230000009368 gene silencing by RNA Effects 0.000 claims description 7
- 238000004519 manufacturing process Methods 0.000 claims description 7
- 230000008506 pathogenesis Effects 0.000 claims description 7
- 102000003893 Histone acetyltransferases Human genes 0.000 claims description 6
- 108090000246 Histone acetyltransferases Proteins 0.000 claims description 6
- 235000007340 Hordeum vulgare Nutrition 0.000 claims description 6
- 240000005979 Hordeum vulgare Species 0.000 claims description 6
- 239000004472 Lysine Substances 0.000 claims description 6
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 6
- 235000011684 Sorghum saccharatum Nutrition 0.000 claims description 6
- 125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 claims description 6
- 241000219198 Brassica Species 0.000 claims description 5
- 101710090647 Histone H2A.Z Proteins 0.000 claims description 5
- 125000000539 amino acid group Chemical group 0.000 claims description 5
- 239000002773 nucleotide Substances 0.000 claims description 5
- 125000003729 nucleotide group Chemical group 0.000 claims description 5
- 230000002028 premature Effects 0.000 claims description 5
- 230000003827 upregulation Effects 0.000 claims description 5
- 235000011331 Brassica Nutrition 0.000 claims description 4
- 235000010469 Glycine max Nutrition 0.000 claims description 4
- 244000068988 Glycine max Species 0.000 claims description 4
- 240000000111 Saccharum officinarum Species 0.000 claims description 4
- 235000007201 Saccharum officinarum Nutrition 0.000 claims description 4
- 244000005700 microbiome Species 0.000 claims description 4
- 244000082988 Secale cereale Species 0.000 claims description 3
- 235000007238 Secale cereale Nutrition 0.000 claims description 3
- 230000006196 deacetylation Effects 0.000 claims description 3
- 238000003381 deacetylation reaction Methods 0.000 claims description 3
- 230000003828 downregulation Effects 0.000 claims description 2
- 230000006195 histone acetylation Effects 0.000 claims description 2
- 240000006394 Sorghum bicolor Species 0.000 claims 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims 2
- 235000005822 corn Nutrition 0.000 claims 2
- BDJRBEYXGGNYIS-UHFFFAOYSA-N Nonanedioid acid Natural products OC(=O)CCCCCCCC(O)=O BDJRBEYXGGNYIS-UHFFFAOYSA-N 0.000 claims 1
- 241000206602 Eukaryota Species 0.000 abstract description 7
- 230000004048 modification Effects 0.000 abstract description 4
- 238000012986 modification Methods 0.000 abstract description 4
- 101710163595 Chaperone protein DnaK Proteins 0.000 description 53
- 101710178376 Heat shock 70 kDa protein Proteins 0.000 description 53
- 101710152018 Heat shock cognate 70 kDa protein Proteins 0.000 description 53
- 108010077544 Chromatin Proteins 0.000 description 43
- 210000003483 chromatin Anatomy 0.000 description 43
- 238000013518 transcription Methods 0.000 description 40
- 230000035897 transcription Effects 0.000 description 40
- 108020004414 DNA Proteins 0.000 description 33
- 102100031468 Actin-related protein 6 Human genes 0.000 description 27
- 101000923097 Homo sapiens Actin-related protein 6 Proteins 0.000 description 27
- 101000964382 Homo sapiens DNA dC->dU-editing enzyme APOBEC-3D Proteins 0.000 description 27
- 241000219194 Arabidopsis Species 0.000 description 25
- 230000008859 change Effects 0.000 description 23
- 238000004458 analytical method Methods 0.000 description 22
- 101150024768 Arp6 gene Proteins 0.000 description 21
- 102000009572 RNA Polymerase II Human genes 0.000 description 21
- 108010009460 RNA Polymerase II Proteins 0.000 description 21
- 108010033040 Histones Proteins 0.000 description 20
- 230000037361 pathway Effects 0.000 description 20
- 230000002103 transcriptional effect Effects 0.000 description 19
- 101000843312 Arabidopsis thaliana Histone H2A variant 1 Proteins 0.000 description 18
- 238000002474 experimental method Methods 0.000 description 17
- BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 description 15
- 108010059724 Micrococcal Nuclease Proteins 0.000 description 14
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 14
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 14
- 230000007423 decrease Effects 0.000 description 13
- 108091034117 Oligonucleotide Proteins 0.000 description 12
- 108700009124 Transcription Initiation Site Proteins 0.000 description 10
- 230000018109 developmental process Effects 0.000 description 10
- 230000006197 histone deacetylation Effects 0.000 description 10
- 230000007246 mechanism Effects 0.000 description 10
- 238000002703 mutagenesis Methods 0.000 description 10
- 231100000350 mutagenesis Toxicity 0.000 description 10
- 230000008447 perception Effects 0.000 description 10
- 108090000765 processed proteins & peptides Proteins 0.000 description 10
- 239000013598 vector Substances 0.000 description 10
- 102000006947 Histones Human genes 0.000 description 9
- 210000004027 cell Anatomy 0.000 description 9
- 239000012634 fragment Substances 0.000 description 9
- 230000006698 induction Effects 0.000 description 9
- 230000006903 response to temperature Effects 0.000 description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 8
- 230000006870 function Effects 0.000 description 8
- 235000009973 maize Nutrition 0.000 description 8
- 230000001404 mediated effect Effects 0.000 description 8
- 235000009697 arginine Nutrition 0.000 description 7
- 230000027455 binding Effects 0.000 description 7
- 238000011161 development Methods 0.000 description 7
- 230000001939 inductive effect Effects 0.000 description 7
- 230000001133 acceleration Effects 0.000 description 6
- 230000001276 controlling effect Effects 0.000 description 6
- 230000000670 limiting effect Effects 0.000 description 6
- 230000007704 transition Effects 0.000 description 6
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 5
- 244000062793 Sorghum vulgare Species 0.000 description 5
- 150000001484 arginines Chemical class 0.000 description 5
- 238000003556 assay Methods 0.000 description 5
- 230000033228 biological regulation Effects 0.000 description 5
- 230000000875 corresponding effect Effects 0.000 description 5
- 244000038559 crop plants Species 0.000 description 5
- 230000003993 interaction Effects 0.000 description 5
- 230000035772 mutation Effects 0.000 description 5
- 239000013612 plasmid Substances 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 238000003753 real-time PCR Methods 0.000 description 5
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 4
- 240000002791 Brassica napus Species 0.000 description 4
- 235000006008 Brassica napus var napus Nutrition 0.000 description 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
- 102000018754 Histone Chaperones Human genes 0.000 description 4
- 108010052497 Histone Chaperones Proteins 0.000 description 4
- 229940124158 Protease/peptidase inhibitor Drugs 0.000 description 4
- 230000006399 behavior Effects 0.000 description 4
- 230000029087 digestion Effects 0.000 description 4
- 230000002068 genetic effect Effects 0.000 description 4
- 238000010348 incorporation Methods 0.000 description 4
- 108091070501 miRNA Proteins 0.000 description 4
- 239000002679 microRNA Substances 0.000 description 4
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 4
- 238000002741 site-directed mutagenesis Methods 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 230000009466 transformation Effects 0.000 description 4
- 230000009261 transgenic effect Effects 0.000 description 4
- 108700028369 Alleles Proteins 0.000 description 3
- 108091093088 Amplicon Proteins 0.000 description 3
- 101000843287 Arabidopsis thaliana Probable histone H2A variant 3 Proteins 0.000 description 3
- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 description 3
- 235000017647 Brassica oleracea var italica Nutrition 0.000 description 3
- 240000003259 Brassica oleracea var. botrytis Species 0.000 description 3
- 241000195940 Bryophyta Species 0.000 description 3
- 229920000742 Cotton Polymers 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- 241000233866 Fungi Species 0.000 description 3
- 244000299507 Gossypium hirsutum Species 0.000 description 3
- 101150031823 HSP70 gene Proteins 0.000 description 3
- 102100031880 Helicase SRCAP Human genes 0.000 description 3
- 102100033636 Histone H3.2 Human genes 0.000 description 3
- 101000704158 Homo sapiens Helicase SRCAP Proteins 0.000 description 3
- 235000003228 Lactuca sativa Nutrition 0.000 description 3
- 240000008415 Lactuca sativa Species 0.000 description 3
- 235000004431 Linum usitatissimum Nutrition 0.000 description 3
- 240000006240 Linum usitatissimum Species 0.000 description 3
- 108060001084 Luciferase Proteins 0.000 description 3
- 239000005089 Luciferase Substances 0.000 description 3
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 3
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 3
- 244000046052 Phaseolus vulgaris Species 0.000 description 3
- 241000209504 Poaceae Species 0.000 description 3
- 241000219000 Populus Species 0.000 description 3
- 240000003768 Solanum lycopersicum Species 0.000 description 3
- 108091023040 Transcription factor Proteins 0.000 description 3
- 102000040945 Transcription factor Human genes 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 210000000349 chromosome Anatomy 0.000 description 3
- 230000002950 deficient Effects 0.000 description 3
- 230000003111 delayed effect Effects 0.000 description 3
- 230000008021 deposition Effects 0.000 description 3
- 238000009826 distribution Methods 0.000 description 3
- 230000035784 germination Effects 0.000 description 3
- 229910052588 hydroxylapatite Inorganic materials 0.000 description 3
- 230000000366 juvenile effect Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000002493 microarray Methods 0.000 description 3
- 230000002018 overexpression Effects 0.000 description 3
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 3
- 238000005215 recombination Methods 0.000 description 3
- 230000006798 recombination Effects 0.000 description 3
- 230000035882 stress Effects 0.000 description 3
- 238000012225 targeting induced local lesions in genomes Methods 0.000 description 3
- 241000589158 Agrobacterium Species 0.000 description 2
- 244000291564 Allium cepa Species 0.000 description 2
- 235000002732 Allium cepa var. cepa Nutrition 0.000 description 2
- 108700021316 Arabidopsis H2A.Z Proteins 0.000 description 2
- 101100016295 Arabidopsis thaliana H2AV gene Proteins 0.000 description 2
- 101100177160 Arabidopsis thaliana HAC2 gene Proteins 0.000 description 2
- 101100177161 Arabidopsis thaliana HAC4 gene Proteins 0.000 description 2
- 101100123408 Arabidopsis thaliana HAC5 gene Proteins 0.000 description 2
- 101000843311 Arabidopsis thaliana Probable histone H2A variant 2 Proteins 0.000 description 2
- 239000004475 Arginine Substances 0.000 description 2
- 108091026821 Artificial microRNA Proteins 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 101001093716 Autographa californica nuclear polyhedrosis virus Per os infectivity factor 4 Proteins 0.000 description 2
- 241000219310 Beta vulgaris subsp. vulgaris Species 0.000 description 2
- 240000007124 Brassica oleracea Species 0.000 description 2
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 2
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 2
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 2
- 241000222122 Candida albicans Species 0.000 description 2
- 101100507655 Canis lupus familiaris HSPA1 gene Proteins 0.000 description 2
- 244000241235 Citrullus lanatus Species 0.000 description 2
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 2
- 230000007067 DNA methylation Effects 0.000 description 2
- 240000005717 Dioscorea alata Species 0.000 description 2
- 235000002723 Dioscorea alata Nutrition 0.000 description 2
- 235000007056 Dioscorea composita Nutrition 0.000 description 2
- 235000009723 Dioscorea convolvulacea Nutrition 0.000 description 2
- 235000005362 Dioscorea floribunda Nutrition 0.000 description 2
- 235000004868 Dioscorea macrostachya Nutrition 0.000 description 2
- 235000005361 Dioscorea nummularia Nutrition 0.000 description 2
- 235000005360 Dioscorea spiculiflora Nutrition 0.000 description 2
- 101150115548 ENTR1 gene Proteins 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241000220485 Fabaceae Species 0.000 description 2
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 2
- 241000228404 Histoplasma capsulatum Species 0.000 description 2
- 101000735465 Homo sapiens Protein mono-ADP-ribosyltransferase PARP6 Proteins 0.000 description 2
- 235000006350 Ipomoea batatas var. batatas Nutrition 0.000 description 2
- 240000004658 Medicago sativa Species 0.000 description 2
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 2
- 241000208125 Nicotiana Species 0.000 description 2
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 2
- 235000010582 Pisum sativum Nutrition 0.000 description 2
- 240000004713 Pisum sativum Species 0.000 description 2
- 102100034932 Protein mono-ADP-ribosyltransferase PARP6 Human genes 0.000 description 2
- 235000004789 Rosa xanthina Nutrition 0.000 description 2
- 241000235343 Saccharomycetales Species 0.000 description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 description 2
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 235000021536 Sugar beet Nutrition 0.000 description 2
- 108090000848 Ubiquitin Proteins 0.000 description 2
- 102000044159 Ubiquitin Human genes 0.000 description 2
- 240000006365 Vitis vinifera Species 0.000 description 2
- 235000014787 Vitis vinifera Nutrition 0.000 description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
- ZSLZBFCDCINBPY-ZSJPKINUSA-N acetyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 ZSLZBFCDCINBPY-ZSJPKINUSA-N 0.000 description 2
- 230000003044 adaptive effect Effects 0.000 description 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 229940095731 candida albicans Drugs 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 230000010259 detection of temperature stimulus Effects 0.000 description 2
- 230000001627 detrimental effect Effects 0.000 description 2
- 235000004879 dioscorea Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000003623 enhancer Substances 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 241001233957 eudicotyledons Species 0.000 description 2
- 210000003527 eukaryotic cell Anatomy 0.000 description 2
- 235000004426 flaxseed Nutrition 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 230000008642 heat stress Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 230000011987 methylation Effects 0.000 description 2
- 238000007069 methylation reaction Methods 0.000 description 2
- 210000004940 nucleus Anatomy 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000003938 response to stress Effects 0.000 description 2
- 230000004043 responsiveness Effects 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- 238000012552 review Methods 0.000 description 2
- 230000014284 seed dormancy process Effects 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 230000035939 shock Effects 0.000 description 2
- 150000003384 small molecules Chemical class 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 230000008685 targeting Effects 0.000 description 2
- 108091006107 transcriptional repressors Proteins 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 230000034512 ubiquitination Effects 0.000 description 2
- 238000010798 ubiquitination Methods 0.000 description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- 102100021324 5-azacytidine-induced protein 2 Human genes 0.000 description 1
- 101150111419 ATH1 gene Proteins 0.000 description 1
- 240000004507 Abelmoschus esculentus Species 0.000 description 1
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 102000007469 Actins Human genes 0.000 description 1
- 102000005869 Activating Transcription Factors Human genes 0.000 description 1
- 108010005254 Activating Transcription Factors Proteins 0.000 description 1
- 235000005254 Allium ampeloprasum Nutrition 0.000 description 1
- 240000006108 Allium ampeloprasum Species 0.000 description 1
- 241000234270 Amaryllidaceae Species 0.000 description 1
- 244000144730 Amygdalus persica Species 0.000 description 1
- 240000001436 Antirrhinum majus Species 0.000 description 1
- 108020005544 Antisense RNA Proteins 0.000 description 1
- 241000219195 Arabidopsis thaliana Species 0.000 description 1
- 101100433747 Arabidopsis thaliana ABCA2 gene Proteins 0.000 description 1
- 101100016301 Arabidopsis thaliana At1g52740 gene Proteins 0.000 description 1
- 101100016298 Arabidopsis thaliana At2g38810 gene Proteins 0.000 description 1
- 101100123333 Arabidopsis thaliana At4g13570 gene Proteins 0.000 description 1
- 101100177154 Arabidopsis thaliana HAC12 gene Proteins 0.000 description 1
- 101100339685 Arabidopsis thaliana HSP70-4 gene Proteins 0.000 description 1
- 101000872100 Arabidopsis thaliana Histone H2A.8 Proteins 0.000 description 1
- 101100288148 Arabidopsis thaliana KNAT5 gene Proteins 0.000 description 1
- 101100135611 Arabidopsis thaliana PAP12 gene Proteins 0.000 description 1
- 101100371686 Arabidopsis thaliana UBQ10 gene Proteins 0.000 description 1
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 241000233788 Arecaceae Species 0.000 description 1
- 241000208838 Asteraceae Species 0.000 description 1
- 101150002428 Atoh1 gene Proteins 0.000 description 1
- 244000075850 Avena orientalis Species 0.000 description 1
- 235000007319 Avena orientalis Nutrition 0.000 description 1
- 235000007558 Avena sp Nutrition 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 241000218999 Begoniaceae Species 0.000 description 1
- 235000014698 Brassica juncea var multisecta Nutrition 0.000 description 1
- 235000011293 Brassica napus Nutrition 0.000 description 1
- 240000000385 Brassica napus var. napus Species 0.000 description 1
- 235000006618 Brassica rapa subsp oleifera Nutrition 0.000 description 1
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 1
- 241000219193 Brassicaceae Species 0.000 description 1
- DPUOLQHDNGRHBS-UHFFFAOYSA-N Brassidinsaeure Natural products CCCCCCCCC=CCCCCCCCCCCCC(O)=O DPUOLQHDNGRHBS-UHFFFAOYSA-N 0.000 description 1
- 101150089330 CHZ1 gene Proteins 0.000 description 1
- 101100057132 Candida albicans (strain SC5314 / ATCC MYA-2876) ATC1 gene Proteins 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241000220244 Capsella <angiosperm> Species 0.000 description 1
- 235000002566 Capsicum Nutrition 0.000 description 1
- 240000008574 Capsicum frutescens Species 0.000 description 1
- 241000701489 Cauliflower mosaic virus Species 0.000 description 1
- 241000871189 Chenopodiaceae Species 0.000 description 1
- 241001478750 Chlorophytum comosum Species 0.000 description 1
- 235000007516 Chrysanthemum Nutrition 0.000 description 1
- 240000005250 Chrysanthemum indicum Species 0.000 description 1
- 235000010523 Cicer arietinum Nutrition 0.000 description 1
- 244000045195 Cicer arietinum Species 0.000 description 1
- 241000207199 Citrus Species 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- 235000021508 Coleus Nutrition 0.000 description 1
- 244000061182 Coleus blumei Species 0.000 description 1
- 241000218631 Coniferophyta Species 0.000 description 1
- 241001275954 Cortinarius caperatus Species 0.000 description 1
- 235000009854 Cucurbita moschata Nutrition 0.000 description 1
- 240000001980 Cucurbita pepo Species 0.000 description 1
- 235000009852 Cucurbita pepo Nutrition 0.000 description 1
- 241000219104 Cucurbitaceae Species 0.000 description 1
- 102100028717 Cytosolic 5'-nucleotidase 3A Human genes 0.000 description 1
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 description 1
- 230000033616 DNA repair Effects 0.000 description 1
- 230000004568 DNA-binding Effects 0.000 description 1
- CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 description 1
- 241001306121 Dracaena <Squamata> Species 0.000 description 1
- 102100025654 Endosome-associated-trafficking regulator 1 Human genes 0.000 description 1
- URXZXNYJPAJJOQ-UHFFFAOYSA-N Erucic acid Natural products CCCCCCC=CCCCCCCCCCCCC(O)=O URXZXNYJPAJJOQ-UHFFFAOYSA-N 0.000 description 1
- 108010022894 Euchromatin Proteins 0.000 description 1
- 235000009419 Fagopyrum esculentum Nutrition 0.000 description 1
- 240000008620 Fagopyrum esculentum Species 0.000 description 1
- 241000234642 Festuca Species 0.000 description 1
- 244000286663 Ficus elastica Species 0.000 description 1
- 235000016623 Fragaria vesca Nutrition 0.000 description 1
- 240000009088 Fragaria x ananassa Species 0.000 description 1
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 1
- 241000208150 Geraniaceae Species 0.000 description 1
- 241000208152 Geranium Species 0.000 description 1
- 101710095129 Granule-bound starch synthase 1 Proteins 0.000 description 1
- 101150114009 HTZ1 gene Proteins 0.000 description 1
- 244000020551 Helianthus annuus Species 0.000 description 1
- 235000003222 Helianthus annuus Nutrition 0.000 description 1
- 108010034791 Heterochromatin Proteins 0.000 description 1
- 102000017286 Histone H2A Human genes 0.000 description 1
- 108050005231 Histone H2A Proteins 0.000 description 1
- 102100039869 Histone H2B type F-S Human genes 0.000 description 1
- 101001056455 Homo sapiens Endosome-associated-trafficking regulator 1 Proteins 0.000 description 1
- 101001035372 Homo sapiens Histone H2B type F-S Proteins 0.000 description 1
- 101000664600 Homo sapiens Tripartite motif-containing protein 3 Proteins 0.000 description 1
- 108090001007 Interleukin-8 Proteins 0.000 description 1
- PMGCQNGBLMMXEW-UHFFFAOYSA-N Isoamyl salicylate Chemical compound CC(C)CCOC(=O)C1=CC=CC=C1O PMGCQNGBLMMXEW-UHFFFAOYSA-N 0.000 description 1
- 235000014647 Lens culinaris subsp culinaris Nutrition 0.000 description 1
- 244000043158 Lens esculenta Species 0.000 description 1
- 241000209510 Liliopsida Species 0.000 description 1
- 240000004296 Lolium perenne Species 0.000 description 1
- DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 description 1
- 241000219745 Lupinus Species 0.000 description 1
- 244000070406 Malus silvestris Species 0.000 description 1
- 241000219071 Malvaceae Species 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 102100025169 Max-binding protein MNT Human genes 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 108700011259 MicroRNAs Proteins 0.000 description 1
- 240000003433 Miscanthus floridulus Species 0.000 description 1
- 241001523998 Moenchia erecta Species 0.000 description 1
- DTERQYGMUDWYAZ-ZETCQYMHSA-N N(6)-acetyl-L-lysine Chemical compound CC(=O)NCCCC[C@H]([NH3+])C([O-])=O DTERQYGMUDWYAZ-ZETCQYMHSA-N 0.000 description 1
- 101100506118 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) hH2Az gene Proteins 0.000 description 1
- 241000208135 Nicotiana sp. Species 0.000 description 1
- 101100338467 Oryctolagus cuniculus HCN4 gene Proteins 0.000 description 1
- 101100175601 Oryza sativa subsp. japonica AGPL1 gene Proteins 0.000 description 1
- 240000007377 Petunia x hybrida Species 0.000 description 1
- 240000008299 Pinus lambertiana Species 0.000 description 1
- 235000008566 Pinus taeda Nutrition 0.000 description 1
- 241000218679 Pinus taeda Species 0.000 description 1
- 241001600128 Populus tremula x Populus alba Species 0.000 description 1
- 235000009827 Prunus armeniaca Nutrition 0.000 description 1
- 244000018633 Prunus armeniaca Species 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- 241000220324 Pyrus Species 0.000 description 1
- 238000001190 Q-PCR Methods 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 102100038042 Retinoblastoma-associated protein Human genes 0.000 description 1
- 101710124357 Retinoblastoma-associated protein Proteins 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 241000109329 Rosa xanthina Species 0.000 description 1
- 241000220222 Rosaceae Species 0.000 description 1
- 244000070968 Saintpaulia ionantha Species 0.000 description 1
- 241000124033 Salix Species 0.000 description 1
- 108091027967 Small hairpin RNA Proteins 0.000 description 1
- 241000208292 Solanaceae Species 0.000 description 1
- 235000009337 Spinacia oleracea Nutrition 0.000 description 1
- 244000300264 Spinacia oleracea Species 0.000 description 1
- 102100029373 Transcription factor ATOH1 Human genes 0.000 description 1
- 108700019146 Transgenes Proteins 0.000 description 1
- 241000219793 Trifolium Species 0.000 description 1
- 102100038798 Tripartite motif-containing protein 3 Human genes 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 235000002096 Vicia faba var. equina Nutrition 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 108020002494 acetyltransferase Proteins 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 230000031016 anaphase Effects 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 239000002551 biofuel Substances 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000004566 building material Substances 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
- 230000028956 calcium-mediated signaling Effects 0.000 description 1
- 239000001390 capsicum minimum Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000002487 chromatin immunoprecipitation Methods 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 239000003184 complementary RNA Substances 0.000 description 1
- 238000004624 confocal microscopy Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000007771 core particle Substances 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 229940009976 deoxycholate Drugs 0.000 description 1
- KXGVEGMKQFWNSR-LLQZFEROSA-N deoxycholic acid Chemical compound C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 KXGVEGMKQFWNSR-LLQZFEROSA-N 0.000 description 1
- 230000003831 deregulation Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 235000005489 dwarf bean Nutrition 0.000 description 1
- 244000013123 dwarf bean Species 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000002922 epistatic effect Effects 0.000 description 1
- DPUOLQHDNGRHBS-KTKRTIGZSA-N erucic acid Chemical compound CCCCCCCC\C=C/CCCCCCCCCCCC(O)=O DPUOLQHDNGRHBS-KTKRTIGZSA-N 0.000 description 1
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 1
- 210000000632 euchromatin Anatomy 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 238000005429 filling process Methods 0.000 description 1
- 108020001507 fusion proteins Proteins 0.000 description 1
- 102000037865 fusion proteins Human genes 0.000 description 1
- 230000030279 gene silencing Effects 0.000 description 1
- 238000012252 genetic analysis Methods 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 210000004458 heterochromatin Anatomy 0.000 description 1
- 230000009618 hypocotyl growth Effects 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 235000021374 legumes Nutrition 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- -1 lysine amino acids Chemical class 0.000 description 1
- 239000012139 lysis buffer Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 235000019713 millet Nutrition 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 238000009304 pastoral farming Methods 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 235000021017 pears Nutrition 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 230000006461 physiological response Effects 0.000 description 1
- 230000008121 plant development Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 230000012743 protein tagging Effects 0.000 description 1
- 238000004537 pulping Methods 0.000 description 1
- 239000013014 purified material Substances 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000009790 rate-determining step (RDS) Methods 0.000 description 1
- 230000037425 regulation of transcription Effects 0.000 description 1
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000033458 reproduction Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000009758 senescence Effects 0.000 description 1
- 238000002864 sequence alignment Methods 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 239000004460 silage Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 235000020354 squash Nutrition 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8237—Externally regulated expression systems
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
- C12N15/827—Flower development or morphology, e.g. flowering promoting factor [FPF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Physiology (AREA)
- Botany (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Radiation Pyrometers (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1000184.0A GB201000184D0 (en) | 2010-01-07 | 2010-01-07 | Methods and compositions for altering temperature sensing in eukaryotic organisms |
| GB1000184.0 | 2010-01-07 | ||
| PCT/GB2010/050956 WO2011083290A1 (en) | 2010-01-07 | 2010-06-08 | Methods and compositions for altering temperature sensing in eukaryotic organisms |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2010340802A1 AU2010340802A1 (en) | 2012-06-21 |
| AU2010340802B2 true AU2010340802B2 (en) | 2014-03-13 |
Family
ID=41819024
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2010340802A Ceased AU2010340802B2 (en) | 2010-01-07 | 2010-06-08 | Methods and compositions for altering temperature sensing in eukaryotic organisms |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US20130239252A1 (enExample) |
| EP (1) | EP2521734B1 (enExample) |
| CN (1) | CN102781956B (enExample) |
| AR (1) | AR079319A1 (enExample) |
| AU (1) | AU2010340802B2 (enExample) |
| BR (1) | BR112012016796A2 (enExample) |
| CA (1) | CA2786341A1 (enExample) |
| GB (1) | GB201000184D0 (enExample) |
| IN (1) | IN2012DN06570A (enExample) |
| MX (1) | MX2012007867A (enExample) |
| WO (2) | WO2011083290A1 (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ735257A (en) | 2013-11-04 | 2018-09-28 | Dow Agrosciences Llc | Optimal soybean loci |
| WO2015066636A2 (en) | 2013-11-04 | 2015-05-07 | Dow Agrosciences Llc | Optimal maize loci |
| CN107223156A (zh) * | 2013-11-04 | 2017-09-29 | 美国陶氏益农公司 | 最优玉米座位 |
| CA2926822C (en) | 2013-11-04 | 2022-12-13 | Dow Agrosciences Llc | Optimal soybean loci |
| CN104894142A (zh) * | 2015-06-11 | 2015-09-09 | 中国科学院西北高原生物研究所 | 调控花青素合成与代谢的小麦新基因TaMYB7D |
| EP3712271A1 (en) | 2019-03-18 | 2020-09-23 | Commissariat à l'Energie Atomique et aux Energies Alternatives | Altering thermoresponsive growth in plants via genome editing of phytochrome interacting factor 4 (pif4) regulatory elements |
| CN110938605B (zh) * | 2019-11-20 | 2021-05-04 | 中山大学附属第五医院 | 一种体外组装的HBV cccDNA核小体及其制备方法 |
| CN111153980B (zh) * | 2020-02-17 | 2022-03-15 | 南京农业大学 | 一种植物粒型相关蛋白OsSDSG及其编码基因与应用 |
| CN117965579B (zh) * | 2024-04-02 | 2024-06-07 | 中国科学院遗传与发育生物学研究所 | 麦属特异转座子h2a.1及其应用 |
| WO2025233441A1 (en) | 2024-05-08 | 2025-11-13 | Gmi - Gregor-Mendel-Institut Für Molekulare Pflanzenbiologie Gmbh | Modified h2a.z l2 region |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2316036A1 (en) * | 1999-08-27 | 2001-02-27 | Keqiang Wu | Repressing gene expression in plants |
| AU2001286811B2 (en) * | 2000-08-24 | 2007-03-01 | Syngenta Participations Ag | Stress-regulated genes of plants, transgenic plants containing same, and methods of use |
-
2010
- 2010-01-07 GB GBGB1000184.0A patent/GB201000184D0/en not_active Ceased
- 2010-06-08 IN IN6570DEN2012 patent/IN2012DN06570A/en unknown
- 2010-06-08 AU AU2010340802A patent/AU2010340802B2/en not_active Ceased
- 2010-06-08 MX MX2012007867A patent/MX2012007867A/es active IP Right Grant
- 2010-06-08 US US13/520,967 patent/US20130239252A1/en not_active Abandoned
- 2010-06-08 BR BR112012016796A patent/BR112012016796A2/pt not_active IP Right Cessation
- 2010-06-08 CN CN201080060911.3A patent/CN102781956B/zh not_active Expired - Fee Related
- 2010-06-08 WO PCT/GB2010/050956 patent/WO2011083290A1/en not_active Ceased
- 2010-06-08 EP EP10724120.0A patent/EP2521734B1/en not_active Not-in-force
- 2010-06-08 CA CA2786341A patent/CA2786341A1/en not_active Abandoned
- 2010-12-07 AR ARP100104519A patent/AR079319A1/es unknown
- 2010-12-17 WO PCT/GB2010/052144 patent/WO2011083298A1/en not_active Ceased
Non-Patent Citations (5)
| Title |
|---|
| ISHIBASHI TOYOTAKA ET AL: "Acetylation of vertebrate H2A.Z and its effect on the structure of the nucleosome."BIOCHEMISTRY 9 JUN 2009, vol. 48, no. 22, 9 June 2009 (2009-06-09) , pages 5007- 5017. * |
| KUMAR S VET AL: "H2A.Z-Containing Nucleosomes Mediate the Thermosensory Response in Arabidopsis" CELL, vol. 140, no. 1, 8 January 2010 (2010-01-08), pages 136-147. available online on 17.12.2009, * |
| MARCH-DfAZ ROSANA ET AL: "The beauty of being a variant: H2A.Z and the SWR1 complex in plants."MOLECULAR PLANT JUL 2009 LNKD- PUBMED:19825639, vol. 2, no. 4, July 2009 (2009-07) , pages 565-577. * |
| MARCH-DIAZ R ET AL: "Histone H2A.Z and homologues of components of the SWR1 complex are required to control immunity in Arabidopsis"PLANT JOURNAL FEBRUARY 2008 BLACKWELL PUBLISHING LTD GB, vol. 53, no. 3, February 2008, pages 475-487 * |
| MILLAR CATHERINE BET AL: "Acetylation of H2AZ Lys 14 is associated with genome-wide gene activity in yeast"GENES & DEVELOPMENT, vol. 20, no. 6, March 2006 (2006-03) , pages 711-722. * |
Also Published As
| Publication number | Publication date |
|---|---|
| AU2010340802A1 (en) | 2012-06-21 |
| US20130239252A1 (en) | 2013-09-12 |
| CN102781956B (zh) | 2015-09-16 |
| MX2012007867A (es) | 2012-08-03 |
| GB201000184D0 (en) | 2010-02-24 |
| EP2521734B1 (en) | 2016-09-14 |
| IN2012DN06570A (enExample) | 2015-10-23 |
| EP2521734A1 (en) | 2012-11-14 |
| AR079319A1 (es) | 2012-01-18 |
| CA2786341A1 (en) | 2011-07-14 |
| CN102781956A (zh) | 2012-11-14 |
| BR112012016796A2 (pt) | 2019-09-24 |
| WO2011083298A1 (en) | 2011-07-14 |
| WO2011083290A1 (en) | 2011-07-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2010340802B2 (en) | Methods and compositions for altering temperature sensing in eukaryotic organisms | |
| Lv et al. | A WRKY transcription factor, FtWRKY46, from Tartary buckwheat improves salt tolerance in transgenic Arabidopsis thaliana | |
| Su et al. | A novel MYBS3-dependent pathway confers cold tolerance in rice | |
| He et al. | The MicroRNA390/TRANS-ACTING SHORT INTERFERING RNA3 module mediates lateral root growth under salt stress via the auxin pathway | |
| Zhou et al. | Rice homeodomain protein WOX11 recruits a histone acetyltransferase complex to establish programs of cell proliferation of crown root meristem | |
| Kang et al. | A snapshot of functional genetic studies in Medicago truncatula | |
| Lin et al. | Role of Arabidopsis RAP2. 4 in regulating light-and ethylene-mediated developmental processes and drought stress tolerance | |
| Brugière et al. | Overexpression of RING domain E3 ligase ZmXerico1 confers drought tolerance through regulation of ABA homeostasis | |
| Cheng et al. | The Arabidopsis ETHYLENE RESPONSE FACTOR1 regulates abiotic stress-responsive gene expression by binding to different cis-acting elements in response to different stress signals | |
| Alonso et al. | A pivotal role of the basic leucine zipper transcription factor bZIP53 in the regulation of Arabidopsis seed maturation gene expression based on heterodimerization and protein complex formation | |
| Carvalho et al. | On the physiological significance of alternative splicing events in higher plants | |
| Kim et al. | Functional characterization of DEAD-box RNA helicases in Arabidopsis thaliana under abiotic stress conditions | |
| Li et al. | A novel NAC transcription factor from Suaeda liaotungensis K. enhanced transgenic Arabidopsis drought, salt, and cold stress tolerance | |
| Kim et al. | Molecular and functional characterization of CaLEA6, the gene for a hydrophobic LEA protein from Capsicum annuum | |
| Gong et al. | Divergent functions of the GAGA‐binding transcription factor family in rice | |
| Wu et al. | Characterization and fine mapping of the rice premature senescence mutant ospse1 | |
| US20140137290A1 (en) | Molecular clock mechanism of hybrid vigor | |
| CN106047893B (zh) | OsCOL16基因在控制水稻抽穗期中的应用 | |
| Jiang et al. | Tomato SlDREB1 gene conferred the transcriptional activation of drought-induced gene and an enhanced tolerance of the transgenic Arabidopsis to drought stress | |
| Li et al. | RsERF40 contributes to cold stress tolerance and cell expansion of taproot in radish (Raphanus sativus L.) | |
| Min et al. | Arabidopsis basic helix-loop-helix 34 (bHLH34) is involved in glucose signaling through binding to a GAGA cis-element | |
| Li et al. | Overexpression of TCP transcription factor OsPCF7 improves agronomic trait in rice | |
| Liu et al. | Suppression of TaHDA8-mediated lysine deacetylation of TaAREB3 acts as a drought adaptive mechanism in wheat root development | |
| Zhou et al. | Comparative genome organization reveals a single copy of CBF in the freezing tolerant crucifer Thlaspi arvense | |
| EP2385128A1 (en) | Modulation of germination, elongation growth and flowering time in plants |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGA | Letters patent sealed or granted (standard patent) | ||
| MK14 | Patent ceased section 143(a) (annual fees not paid) or expired |