AU2010306862B2 - Protein detection via nanoreporters - Google Patents
Protein detection via nanoreporters Download PDFInfo
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- AU2010306862B2 AU2010306862B2 AU2010306862A AU2010306862A AU2010306862B2 AU 2010306862 B2 AU2010306862 B2 AU 2010306862B2 AU 2010306862 A AU2010306862 A AU 2010306862A AU 2010306862 A AU2010306862 A AU 2010306862A AU 2010306862 B2 AU2010306862 B2 AU 2010306862B2
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- protein
- oligo
- nanoreporter
- probe
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
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Priority Applications (1)
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| AU2015261558A AU2015261558B2 (en) | 2009-10-13 | 2015-11-24 | Protein Detection Via Nanoreporters |
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| US25119209P | 2009-10-13 | 2009-10-13 | |
| US61/251,192 | 2009-10-13 | ||
| US32522410P | 2010-04-16 | 2010-04-16 | |
| US61/325,224 | 2010-04-16 | ||
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Country Status (8)
Families Citing this family (135)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3236264A3 (en) | 2009-10-13 | 2017-11-08 | Nanostring Technologies, Inc | Protein detection via nanoreporters |
| EP2534174A4 (en) * | 2010-02-12 | 2015-02-25 | Solulink Inc | Preparation and / or purification of oligonucleotide conjugates |
| JP5893607B2 (ja) | 2010-04-05 | 2016-03-23 | プログノシス バイオサイエンシズ インコーポレイテッドＰｒｏｇｎｏｓｙｓ Ｂｉｏｓｃｉｅｎｃｅｓ，Ｉｎｃ． | 空間コード化生物学的アッセイ |
| US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
| US9523680B2 (en) * | 2010-06-30 | 2016-12-20 | Ambergen, Inc. | Global Proteomic screening of random bead arrays using mass spectrometry imaging |
| EP2622103B2 (en) | 2010-09-30 | 2022-11-16 | Bio-Rad Laboratories, Inc. | Sandwich assays in droplets |
| US9150852B2 (en) | 2011-02-18 | 2015-10-06 | Raindance Technologies, Inc. | Compositions and methods for molecular labeling |
| GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
| EP3604555B1 (en) | 2011-10-14 | 2024-12-25 | President and Fellows of Harvard College | Sequencing by structure assembly |
| EP2769202A4 (en) * | 2011-10-20 | 2015-11-18 | Exxonmobil Upstream Res Co | NANOPARTICLE PROBES, METHODS AND SYSTEMS FOR USE THEREOF |
| US9914958B2 (en) * | 2011-11-05 | 2018-03-13 | President And Fellows Of Harvard College | Nucleic acid-based linkers for detecting and measuring interactions |
| ES2700792T3 (es) * | 2011-11-11 | 2019-02-19 | Eli N Glezer | Procedimientos de ensayo asistidos por coagente de unión |
| ES2991004T3 (es) | 2011-12-22 | 2024-12-02 | Harvard College | Métodos para la detección de analitos |
| WO2014163886A1 (en) | 2013-03-12 | 2014-10-09 | President And Fellows Of Harvard College | Method of generating a three-dimensional nucleic acid containing matrix |
| EP2794928B1 (en) * | 2011-12-22 | 2019-02-20 | President and Fellows of Harvard College | Compositions and methods for analyte detection |
| BR112014019168B1 (pt) | 2012-02-03 | 2021-10-05 | California Institute Of Technology | Método capaz de detectar de forma não degenerada presença ou ausência de analitos em um único volume de amostra e método de detecção da presença ou ausência de cada analito dentre uma pluralidade de analitos |
| US9914967B2 (en) | 2012-06-05 | 2018-03-13 | President And Fellows Of Harvard College | Spatial sequencing of nucleic acids using DNA origami probes |
| EP3901243A1 (en) | 2012-08-03 | 2021-10-27 | California Institute of Technology | Multiplexing and quantification in pcr with reduced hardware and requirements |
| DK3511423T4 (da) | 2012-10-17 | 2024-07-29 | Spatial Transcriptomics Ab | Fremgangsmåder og produkt til optimering af lokaliseret eller rumlig detektion af genekspression i en vævsprøve |
| US10829816B2 (en) | 2012-11-19 | 2020-11-10 | Apton Biosystems, Inc. | Methods of analyte detection |
| JP6395718B2 (ja) | 2012-11-19 | 2018-09-26 | アプトン バイオシステムズ インコーポレイテッド | 単一分子検出を用いた分子分析物のデジタル分析の方法 |
| US10114015B2 (en) | 2013-03-13 | 2018-10-30 | Meso Scale Technologies, Llc. | Assay methods |
| CN110308272B (zh) | 2013-03-13 | 2023-09-12 | 中尺度技术有限责任公司 | 改进的测定方法 |
| MY208492A (en) | 2013-06-04 | 2025-05-13 | President And Fellows Of Harvard College | Rna-guided transcriptional regulation |
| CA2915033C (en) | 2013-06-12 | 2023-08-29 | The General Hospital Corporation | Methods, kits, and systems for multiplexed detection of target molecules and uses thereof |
| EP3013983B1 (en) | 2013-06-25 | 2023-02-15 | Prognosys Biosciences, Inc. | Spatially encoded biological assays using a microfluidic device |
| EP3143165A4 (en) * | 2014-05-15 | 2018-05-16 | Two Pore Guys, Inc. | Scaffold data storage and target detection in a sample using a nanopore |
| KR102757668B1 (ko) | 2014-05-15 | 2025-01-21 | 메소 스케일 테크놀러지즈, 엘엘시 | 개선된 분석 방법 |
| WO2016007839A1 (en) | 2014-07-11 | 2016-01-14 | President And Fellows Of Harvard College | Methods for high-throughput labelling and detection of biological features in situ using microscopy |
| CN119331954A (zh) | 2014-07-30 | 2025-01-21 | 哈佛学院院长及董事 | 探针文库构建 |
| CA2968376C (en) | 2014-11-21 | 2020-06-23 | Nanostring Technologies, Inc. | Enzyme- and amplification-free sequencing |
| CN107249741B (zh) | 2014-11-24 | 2021-04-02 | 纳米线科技公司 | 用于基因纯化和成像的方法和装置 |
| US11198900B2 (en) | 2014-12-06 | 2021-12-14 | Children's Medical Center Corporation | Nucleic acid-based linkers for detecting and measuring interactions |
| ES2935860T3 (es) | 2015-04-10 | 2023-03-13 | Spatial Transcriptomics Ab | Análisis de ácidos nucleicos múltiplex, espacialmente distinguidos de especímenes biológicos |
| US11396650B2 (en) | 2015-06-02 | 2022-07-26 | Children's Medical Center Corporation | Nucleic acid complexes for screening barcoded compounds |
| US12077807B2 (en) | 2015-06-27 | 2024-09-03 | The Research Foundation For The State University Of New York | Compositions and methods for analyte detection using nanoswitches |
| WO2017004394A1 (en) * | 2015-06-30 | 2017-01-05 | Nanostring Technologies, Inc. | Methods and kits for simultaneously detecting gene or protein expression in a plurality of sample types using self-assembling fluorescent barcode nanoreporters |
| CA2992492A1 (en) | 2015-07-17 | 2017-01-26 | Nanostring Technologies, Inc. | Simultaneous quantification of gene expression in a user-defined region of a cross-sectioned tissue |
| AU2016297513B2 (en) | 2015-07-17 | 2021-02-25 | Board Of Regents, The University Of Texas System | Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue |
| CN105132533B (zh) * | 2015-07-24 | 2018-11-23 | 清华大学深圳研究生院 | 一种靶分子浓度的检测方法 |
| CA2997120A1 (en) * | 2015-09-03 | 2017-03-09 | Nanostring Technologies, Inc. | Multivalent probes having single nucleotide resolution |
| EP3371329A4 (en) | 2015-11-03 | 2019-06-19 | President and Fellows of Harvard College | METHOD AND DEVICE FOR VOLUMETRIC IMAGING OF A THREE-DIMENSIONAL NUCLEIC ACID-CONTAINING MATRIX |
| EP4524255A3 (en) | 2015-12-04 | 2025-04-02 | 10X Genomics, Inc. | Methods and compositions for nucleic acid analysis |
| WO2017139409A1 (en) | 2016-02-09 | 2017-08-17 | Children's Medical Center Corporation | Method for detection of analytes via polymer complexes |
| WO2017165585A1 (en) | 2016-03-23 | 2017-09-28 | Children's Medical Center Corporation | Systems and apparatus for detecting compounds in human biological samples |
| WO2017165647A1 (en) | 2016-03-23 | 2017-09-28 | Children's Medical Center Corporation | Rapid and sensitive detection and quantification of analytes in complex samples using polymer-based methods |
| CA3210120C (en) | 2016-04-25 | 2024-04-09 | President And Fellows Of Harvard College | Hybridization chain reaction methods for in situ molecular detection |
| JP7457457B2 (ja) | 2016-05-16 | 2024-03-28 | ナノストリング テクノロジーズ，インコーポレイティド | サンプル中の標的核酸を検出する方法 |
| WO2017218777A1 (en) | 2016-06-17 | 2017-12-21 | California Institute Of Technology | Nucleic acid reactions and related methods and compositions |
| US11352667B2 (en) | 2016-06-21 | 2022-06-07 | 10X Genomics, Inc. | Nucleic acid sequencing |
| DK3264087T3 (da) * | 2016-06-27 | 2020-07-20 | Chimera Biotec Gmbh | Fremgangsmåde og indretning til kvantificering af målmolekyler |
| US9927441B1 (en) | 2016-07-29 | 2018-03-27 | X Development Llc | Combinatorial methods for aptamer based proteomics |
| GB2569252A (en) | 2016-08-31 | 2019-06-12 | Harvard College | Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing |
| CN109983125B (zh) | 2016-08-31 | 2024-06-04 | 哈佛学院董事及会员团体 | 生成用于通过荧光原位测序检测的核酸序列文库的方法 |
| KR102313431B1 (ko) | 2016-11-21 | 2021-10-18 | 나노스트링 테크놀로지스, 인크. | 화학적 조성물 및 이것을 사용하는 방법 |
| KR101968565B1 (ko) | 2017-03-16 | 2019-08-13 | 장준영 | 재킷 임시 걸이부를 구비한 옷걸이 |
| KR102676067B1 (ko) | 2017-03-17 | 2024-06-18 | 퍼시픽 바이오사이언스 오브 캘리포니아, 인크. | 시퀀싱 및 고 해상도 이미징 |
| KR20190140906A (ko) | 2017-04-14 | 2019-12-20 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 세포-유래 마이크로필라멘트 네트워크의 생성 방법 |
| WO2018218150A1 (en) | 2017-05-26 | 2018-11-29 | President And Fellows Of Harvard College | Systems and methods for high-throughput image-based screening |
| US11674951B2 (en) | 2017-07-17 | 2023-06-13 | The Brigham And Women's Hospital, Inc. | Methods for identifying a treatment for rheumatoid arthritis |
| CN120210336A (zh) | 2017-10-06 | 2025-06-27 | 10X基因组学有限公司 | Rna模板化连接 |
| WO2019100080A1 (en) | 2017-11-20 | 2019-05-23 | Children's Medical Center Corporation | Force-controlled nanoswitch assays for single-molecule detection in complex biological fluids |
| WO2019148001A1 (en) * | 2018-01-25 | 2019-08-01 | Apton Biosystems, Inc. | Methods and composition for high throughput single molecule protein detection systems |
| SG11202007501SA (en) | 2018-02-12 | 2020-09-29 | Nanostring Technologies Inc | Biomolecular probes and methods of detecting gene and protein expression |
| US10871485B2 (en) | 2018-04-13 | 2020-12-22 | Rarecyte, Inc. | Kits for labeling of biomarkers and methods of using the same |
| WO2019213478A1 (en) | 2018-05-04 | 2019-11-07 | Nanostring Technologies, Inc. | Gene expression assay for measurement of dna mismatch repair deficiency |
| CA3099909A1 (en) | 2018-05-14 | 2019-11-21 | Nanostring Technologies, Inc. | Chemical compositions and methods of using same |
| JP2021524744A (ja) | 2018-05-21 | 2021-09-16 | ナノストリング テクノロジーズ，インコーポレイティド | 分子遺伝子シグネチャーとその使用方法 |
| US12203129B2 (en) | 2018-07-03 | 2025-01-21 | ChromaCode, Inc. | Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays |
| WO2020028194A1 (en) | 2018-07-30 | 2020-02-06 | Readcoor, Inc. | Methods and systems for sample processing or analysis |
| US11519033B2 (en) | 2018-08-28 | 2022-12-06 | 10X Genomics, Inc. | Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample |
| EP3853382A4 (en) | 2018-09-19 | 2022-06-22 | Apton Biosystems, Inc. | Densely-packed analyte layers and detection methods |
| WO2020076976A1 (en) | 2018-10-10 | 2020-04-16 | Readcoor, Inc. | Three-dimensional spatial molecular indexing |
| CN109337909B (zh) * | 2018-11-12 | 2020-10-13 | 湖南大学 | 一种检测肝癌耐药细胞株的核酸适体及其应用 |
| EP3894588A1 (en) | 2018-12-10 | 2021-10-20 | 10X Genomics, Inc. | Resolving spatial arrays using deconvolution |
| US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| EP3976820A1 (en) | 2019-05-30 | 2022-04-06 | 10X Genomics, Inc. | Methods of detecting spatial heterogeneity of a biological sample |
| CN119351523A (zh) | 2019-05-31 | 2025-01-24 | 10X基因组学有限公司 | 检测目标核酸分子的方法 |
| WO2021092433A2 (en) | 2019-11-08 | 2021-05-14 | 10X Genomics, Inc. | Enhancing specificity of analyte binding |
| IT201900024159A1 (it) | 2019-12-16 | 2021-06-16 | Menarini Silicon Biosystems Spa | Metodo e kit per amplificazione totale del genoma e analisi di molecole bersaglio in un campione biologico |
| GB201919032D0 (en) | 2019-12-20 | 2020-02-05 | Cartana Ab | Method of detecting an analyte |
| EP4219754B1 (en) | 2019-12-23 | 2024-05-15 | 10X Genomics, Inc. | Methods for spatial analysis using rna-templated ligation |
| EP4081656A1 (en) | 2019-12-23 | 2022-11-02 | 10X Genomics, Inc. | Compositions and methods for using fixed biological samples in partition-based assays |
| US12365942B2 (en) | 2020-01-13 | 2025-07-22 | 10X Genomics, Inc. | Methods of decreasing background on a spatial array |
| US12405264B2 (en) | 2020-01-17 | 2025-09-02 | 10X Genomics, Inc. | Electrophoretic system and method for analyte capture |
| US11732299B2 (en) | 2020-01-21 | 2023-08-22 | 10X Genomics, Inc. | Spatial assays with perturbed cells |
| US11702693B2 (en) | 2020-01-21 | 2023-07-18 | 10X Genomics, Inc. | Methods for printing cells and generating arrays of barcoded cells |
| US20210230681A1 (en) | 2020-01-24 | 2021-07-29 | 10X Genomics, Inc. | Methods for spatial analysis using proximity ligation |
| US12076701B2 (en) | 2020-01-31 | 2024-09-03 | 10X Genomics, Inc. | Capturing oligonucleotides in spatial transcriptomics |
| US12110541B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Methods for preparing high-resolution spatial arrays |
| US11898205B2 (en) | 2020-02-03 | 2024-02-13 | 10X Genomics, Inc. | Increasing capture efficiency of spatial assays |
| US12110548B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Bi-directional in situ analysis |
| US11732300B2 (en) | 2020-02-05 | 2023-08-22 | 10X Genomics, Inc. | Increasing efficiency of spatial analysis in a biological sample |
| WO2021158925A1 (en) | 2020-02-07 | 2021-08-12 | 10X Genomics, Inc. | Quantitative and automated permeabilization performance evaluation for spatial transcriptomics |
| US12281357B1 (en) | 2020-02-14 | 2025-04-22 | 10X Genomics, Inc. | In situ spatial barcoding |
| CN115485391A (zh) | 2020-02-17 | 2022-12-16 | 10X基因组学有限公司 | 染色质相互作用的原位分析 |
| CA3168202A1 (en) | 2020-02-21 | 2021-08-26 | Felice Alessio BAVA | Methods and compositions for integrated in situ spatial assay |
| US11891654B2 (en) | 2020-02-24 | 2024-02-06 | 10X Genomics, Inc. | Methods of making gene expression libraries |
| US12188085B2 (en) | 2020-03-05 | 2025-01-07 | 10X Genomics, Inc. | Three-dimensional spatial transcriptomics with sequencing readout |
| EP4139485B1 (en) | 2020-04-22 | 2023-09-06 | 10X Genomics, Inc. | Methods for spatial analysis using targeted rna depletion |
| EP4414459B1 (en) | 2020-05-22 | 2025-09-03 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
| EP4153776B1 (en) | 2020-05-22 | 2025-03-05 | 10X Genomics, Inc. | Spatial analysis to detect sequence variants |
| US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
| WO2021252499A1 (en) | 2020-06-08 | 2021-12-16 | 10X Genomics, Inc. | Methods of determining a surgical margin and methods of use thereof |
| US12209273B2 (en) | 2020-06-12 | 2025-01-28 | 10X Genomics, Inc. | Nucleic acid assays using click chemistry bioconjugation |
| EP4450639A3 (en) | 2020-06-25 | 2025-01-22 | 10X Genomics, Inc. | Spatial analysis of dna methylation |
| US11761038B1 (en) | 2020-07-06 | 2023-09-19 | 10X Genomics, Inc. | Methods for identifying a location of an RNA in a biological sample |
| US11981960B1 (en) | 2020-07-06 | 2024-05-14 | 10X Genomics, Inc. | Spatial analysis utilizing degradable hydrogels |
| US12209280B1 (en) | 2020-07-06 | 2025-01-28 | 10X Genomics, Inc. | Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis |
| US12297499B2 (en) | 2020-08-17 | 2025-05-13 | 10X Genomics, Inc. | Multicomponent nucleic acid probes for sample analysis |
| US20220049303A1 (en) | 2020-08-17 | 2022-02-17 | Readcoor, Llc | Methods and systems for spatial mapping of genetic variants |
| US11981958B1 (en) | 2020-08-20 | 2024-05-14 | 10X Genomics, Inc. | Methods for spatial analysis using DNA capture |
| US11926822B1 (en) | 2020-09-23 | 2024-03-12 | 10X Genomics, Inc. | Three-dimensional spatial analysis |
| US12071667B2 (en) | 2020-11-04 | 2024-08-27 | 10X Genomics, Inc. | Sequence analysis using meta-stable nucleic acid molecules |
| US11827935B1 (en) | 2020-11-19 | 2023-11-28 | 10X Genomics, Inc. | Methods for spatial analysis using rolling circle amplification and detection probes |
| WO2022140028A1 (en) | 2020-12-21 | 2022-06-30 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
| US12060603B2 (en) | 2021-01-19 | 2024-08-13 | 10X Genomics, Inc. | Methods for internally controlled in situ assays using padlock probes |
| US12275984B2 (en) | 2021-03-02 | 2025-04-15 | 10X Genomics, Inc. | Sequential hybridization and quenching |
| EP4428246A3 (en) | 2021-04-14 | 2024-11-27 | 10X Genomics, Inc. | Methods of measuring mislocalization of an analyte |
| EP4320271B1 (en) | 2021-05-06 | 2025-03-19 | 10X Genomics, Inc. | Methods for increasing resolution of spatial analysis |
| ES3030033T3 (en) | 2021-06-03 | 2025-06-26 | 10X Genomics Inc | Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis |
| CN117730161A (zh) | 2021-07-30 | 2024-03-19 | 10X基因组学有限公司 | 用于原位同步反应的方法和组合物 |
| US12139751B2 (en) | 2021-07-30 | 2024-11-12 | 10X Genomics, Inc. | Circularizable probes for in situ analysis |
| US12391984B2 (en) | 2021-08-03 | 2025-08-19 | 10X Genomics, Inc. | Compositions and methods for rolling circle amplification |
| EP4509614A3 (en) | 2021-09-01 | 2025-05-14 | 10X Genomics, Inc. | Methods, compositions, and kits for blocking a capture probe on a spatial array |
| EP4419707A1 (en) | 2021-11-10 | 2024-08-28 | 10X Genomics, Inc. | Methods, compositions, and kits for determining the location of an analyte in a biological sample |
| EP4305195A2 (en) | 2021-12-01 | 2024-01-17 | 10X Genomics, Inc. | Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis |
| EP4490737A1 (en) | 2022-03-08 | 2025-01-15 | 10X Genomics, Inc. | In situ code design methods for minimizing optical crowding |
| EP4505177A1 (en) | 2022-04-01 | 2025-02-12 | 10x Genomics, Inc. | Compositions and methods for targeted masking of autofluorescence |
| EP4522769A1 (en) | 2022-05-11 | 2025-03-19 | 10X Genomics, Inc. | Compositions and methods for in situ sequencing |
| EP4540607A1 (en) | 2022-06-17 | 2025-04-23 | 10X Genomics, Inc. | Catalytic de-crosslinking of samples for in situ analysis |
| EP4511510A1 (en) | 2022-08-16 | 2025-02-26 | 10X Genomics, Inc. | Ap50 polymerases and uses thereof |
| US12319956B2 (en) | 2023-07-31 | 2025-06-03 | 10X Genomics, Inc. | Methods and systems for targeted RNA cleavage and target RNA-primed rolling circle amplification |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6268147B1 (en) * | 1998-11-02 | 2001-07-31 | Kenneth Loren Beattie | Nucleic acid analysis using sequence-targeted tandem hybridization |
| WO2003003810A2 (en) * | 2001-07-03 | 2003-01-16 | The Institute For Systems Biology | Methods for detection and quantification of analytes in complex mixtures |
| WO2005071401A2 (en) * | 2004-01-15 | 2005-08-04 | Chiron Corporation | Homogeneous multiplex assay for nucleic acid targets |
| WO2007076128A2 (en) * | 2005-12-23 | 2007-07-05 | Nanostring Technologies, Inc. | Nanoreporters and methods of manufacturing and use thereof |
| WO2008124847A2 (en) * | 2007-04-10 | 2008-10-16 | Nanostring Technologies, Inc. | Methods and computer systems for identifying target-specific sequences for use in nanoreporters |
Family Cites Families (72)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU622426B2 (en) | 1987-12-11 | 1992-04-09 | Abbott Laboratories | Assay using template-dependent nucleic acid probe reorganization |
| EP0425563B1 (en) | 1988-07-20 | 1996-05-15 | David Segev | Process for amplifying and detecting nucleic acid sequences |
| US5185243A (en) * | 1988-08-25 | 1993-02-09 | Syntex (U.S.A.) Inc. | Method for detection of specific nucleic acid sequences |
| AU635105B2 (en) | 1990-01-26 | 1993-03-11 | Abbott Laboratories | Improved method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions |
| US5320814A (en) | 1991-01-25 | 1994-06-14 | Trustees Of Tufts College | Fiber optic array sensors, apparatus, and methods for concurrently visualizing and chemically detecting multiple analytes of interest in a fluid sample |
| US5543838A (en) | 1993-08-31 | 1996-08-06 | Xerox Corporation | Signal multiplexing system for an image sensor array |
| SE9400088D0 (sv) | 1994-01-14 | 1994-01-14 | Kabi Pharmacia Ab | Bacterial receptor structures |
| AU6898296A (en) | 1995-08-14 | 1997-03-12 | Ely Michael Rabani | Methods and devices for parallel multiplex polynucleotide sequencing |
| DE69638321D1 (de) | 1995-10-11 | 2011-03-03 | Luminex Corp | Gleichzeitige mehrfachanalyse klinischer proben |
| US7144119B2 (en) | 1996-04-25 | 2006-12-05 | Bioarray Solutions Ltd. | System and method for programmable illumination pattern generation |
| US6387707B1 (en) | 1996-04-25 | 2002-05-14 | Bioarray Solutions | Array Cytometry |
| US6416960B1 (en) | 1996-08-08 | 2002-07-09 | Prolume, Ltd. | Detection and visualization of neoplastic tissues and other tissues |
| WO1998026095A1 (en) | 1996-12-10 | 1998-06-18 | Genetrace Systems Inc. | Releasable nonvolatile mass-label molecules |
| US20020034737A1 (en) | 1997-03-04 | 2002-03-21 | Hyseq, Inc. | Methods and compositions for detection or quantification of nucleic acid species |
| US6180415B1 (en) | 1997-02-20 | 2001-01-30 | The Regents Of The University Of California | Plasmon resonant particles, methods and apparatus |
| US20050037397A1 (en) * | 2001-03-28 | 2005-02-17 | Nanosphere, Inc. | Bio-barcode based detection of target analytes |
| US7348181B2 (en) | 1997-10-06 | 2008-03-25 | Trustees Of Tufts College | Self-encoding sensor with microspheres |
| US7115884B1 (en) | 1997-10-06 | 2006-10-03 | Trustees Of Tufts College | Self-encoding fiber optic sensor |
| WO1999019515A1 (en) | 1997-10-14 | 1999-04-22 | Luminex Corporation | Precision fluorescently dyed particles and methods of making and using same |
| US6207392B1 (en) | 1997-11-25 | 2001-03-27 | The Regents Of The University Of California | Semiconductor nanocrystal probes for biological applications and process for making and using such probes |
| WO1999037663A1 (en) | 1998-01-27 | 1999-07-29 | California Institute Of Technology | Method of detecting a nucleic acid |
| WO1999067641A2 (en) | 1998-06-24 | 1999-12-29 | Illumina, Inc. | Decoding of array sensors with microspheres |
| EP0990903B1 (en) | 1998-09-18 | 2003-03-12 | Massachusetts Institute Of Technology | Biological applications of semiconductor nanocrystals |
| WO2000034527A2 (en) | 1998-12-11 | 2000-06-15 | The Regents Of The University Of California | Targeted molecular bar codes |
| US6429027B1 (en) | 1998-12-28 | 2002-08-06 | Illumina, Inc. | Composite arrays utilizing microspheres |
| US6908737B2 (en) | 1999-04-15 | 2005-06-21 | Vitra Bioscience, Inc. | Systems and methods of conducting multiplexed experiments |
| US6355431B1 (en) | 1999-04-20 | 2002-03-12 | Illumina, Inc. | Detection of nucleic acid amplification reactions using bead arrays |
| DE60042738D1 (de) | 1999-05-07 | 2009-09-24 | Life Technologies Corp | Verfahren zum nachweis von analyten mit hilfe von halbleiternanokrystallen |
| US20020051971A1 (en) | 1999-05-21 | 2002-05-02 | John R. Stuelpnagel | Use of microfluidic systems in the detection of target analytes using microsphere arrays |
| AU5764500A (en) | 1999-06-25 | 2001-01-31 | Motorola, Inc. | Novel methods and products for arrayed microsphere analysis |
| US7214477B1 (en) | 1999-07-26 | 2007-05-08 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Layered device with capture regions for cellular analysis |
| JP4137444B2 (ja) | 1999-07-26 | 2008-08-20 | ザ ガバメント オブ ザ ユナイテッドステイツ オブ アメリカ アズ リプレゼンテッド バイ ザ セクレタリー デパートメント オブ ヘルス アンド ヒューマン サービシーズ ザ ナショナル インステ | 細胞分析用の捕捉領域を有する層状装置 |
| CA2382436C (en) | 1999-08-30 | 2011-05-17 | Illumina, Inc. | Methods for improving signal detection from an array |
| US6690470B1 (en) | 1999-11-04 | 2004-02-10 | Arcturus Engineering, Inc. | Automated laser capture microdissection |
| WO2001057268A2 (en) | 2000-02-07 | 2001-08-09 | Illumina, Inc. | Nucleic acid detection methods using universal priming |
| US20020039728A1 (en) | 2000-02-10 | 2002-04-04 | Robert Kain | Alternative substrates and formats for bead-based array of arrays |
| US20020028457A1 (en) | 2000-02-16 | 2002-03-07 | Quantum Dot Corporation | Single target counting assays using semiconductor nanocrystals |
| CN1416365A (zh) | 2000-02-22 | 2003-05-07 | 基因谱公司 | 微阵列制造技术及设备 |
| US20030186426A1 (en) | 2000-03-15 | 2003-10-02 | The Regents Of The University Of California | Multichannel flow cell for interacting single optically trapped, DNA molecules with different chemical species |
| US6759235B2 (en) | 2000-04-06 | 2004-07-06 | Quantum Dot Corporation | Two-dimensional spectral imaging system |
| AU2001278133A1 (en) | 2000-08-01 | 2002-02-13 | Surromed, Inc. | Methods for solid phase nanoextraction and desorption |
| US6777244B2 (en) | 2000-12-06 | 2004-08-17 | Hrl Laboratories, Llc | Compact sensor using microcavity structures |
| US7553619B2 (en) | 2002-02-08 | 2009-06-30 | Qiagen Gmbh | Detection method using dissociated rolling circle amplification |
| US20040229294A1 (en) | 2002-05-21 | 2004-11-18 | Po-Ying Chan-Hui | ErbB surface receptor complexes as biomarkers |
| EP2348125B1 (en) | 2002-10-30 | 2017-06-21 | Nuevolution A/S | Method for the synthesis of a bifunctional complex |
| DE602004021185D1 (de) | 2003-06-27 | 2009-07-02 | Nanosphere Inc | Auf bio-barcodes beruhender nachweis von zielanalyten |
| US7402398B2 (en) | 2003-07-17 | 2008-07-22 | Monogram Biosciences, Inc. | Measuring receptor homodimerization |
| US20050048498A1 (en) | 2003-08-29 | 2005-03-03 | Applera Corporation | Compositions, methods, and kits for assembling probes |
| WO2005037071A2 (en) | 2003-10-14 | 2005-04-28 | Monogram Biosciences, Inc. | Receptor tyrosine kinase signaling pathway analysis for diagnosis and therapy |
| EP1725572B1 (de) | 2003-11-05 | 2017-05-31 | AGCT GmbH | Makromolekulare nukleotidverbindungen und methoden zu deren anwendung |
| TW200526778A (en) | 2003-11-14 | 2005-08-16 | Sembiosys Genetics Inc | Methods for the production of apolipoproteins in transgenic plants |
| US7569392B2 (en) | 2004-01-08 | 2009-08-04 | Vanderbilt University | Multiplex spatial profiling of gene expression |
| US7588906B2 (en) | 2004-02-24 | 2009-09-15 | Wisconsin Alumni Research Foundation | Hydrogels for biomolecule analysis and corresponding method to analyze biomolecules |
| JP2005257641A (ja) * | 2004-03-15 | 2005-09-22 | Geneticlab Co Ltd | Ｒｎａポリメラーゼを用いて複数の蛋白質を同時に検出する方法 |
| EP1889047B1 (en) | 2005-06-07 | 2011-08-10 | Centre National De La Recherche Scientifique -Cnrs- | Use of conjugates with linkers cleavable by photodissociation or fragmentation for mass spectrometry analysis of tissue sections |
| US9127302B2 (en) * | 2005-09-20 | 2015-09-08 | Janssen Diagnostics, Llc | System for the detection and enumeration of suspect target cells in a mixed cell population |
| WO2007076132A2 (en) | 2005-12-23 | 2007-07-05 | Nanostring Technologies, Inc. | Compositions comprising oriented, immobilized macromolecules and methods for their preparation |
| US7855057B2 (en) | 2006-03-23 | 2010-12-21 | Millipore Corporation | Protein splice variant/isoform discrimination and quantitative measurements thereof |
| US8003312B2 (en) | 2007-02-16 | 2011-08-23 | The Board Of Trustees Of The Leland Stanford Junior University | Multiplex cellular assays using detectable cell barcodes |
| US7728287B2 (en) | 2007-03-01 | 2010-06-01 | Lawrence Livermore National Security, Llc | Imaging mass spectrometer with mass tags |
| DK2132339T3 (da) * | 2007-04-04 | 2011-06-27 | Chimera Biotec Gmbh | Fremgangsmåde til detektering af en analyt i en biologisk matrix |
| US8906700B2 (en) | 2007-11-06 | 2014-12-09 | Ambergen, Inc. | Methods and compositions for phototransfer |
| AU2009281836B2 (en) * | 2008-08-14 | 2015-02-19 | Bruker Spatial Biology, Inc. | Stable nanoreporters |
| EP2163900A1 (en) | 2008-09-04 | 2010-03-17 | Commissariat A L'energie Atomique | New method of imaging by mass spectrometry and new mass tag associated trityl derivatives |
| US8309306B2 (en) | 2008-11-12 | 2012-11-13 | Nodality, Inc. | Detection composition |
| DE102008062372B3 (de) | 2008-12-17 | 2010-06-17 | Medizinische Hochschule Hannover | Nachweiskonjugat und Verfahren zur Analyse |
| BRPI1007321A2 (pt) | 2009-01-15 | 2018-07-10 | Laboratory Corp America Holdings | métodos para medir e/ou quantificar a presença e/ou quantidade de her-3 ou her-3 em um complexo em uma amostra de um paciente, e para determinar se um indivíduo com um câncer é provável de responder ao tratamento com uma terapia alvejada, e, anticorpo. |
| JP5457222B2 (ja) | 2009-02-25 | 2014-04-02 | エフ．ホフマン−ラ ロシュ アーゲー | 小型化ハイスループット核酸分析 |
| WO2010115089A2 (en) | 2009-04-03 | 2010-10-07 | University Of Virginia Patent Foundation | Serial multiple antigen colocalization in paraffin-embedded tissue |
| DE102009002653B3 (de) | 2009-04-27 | 2010-05-27 | Federal-Mogul Nürnberg GmbH | Verfahren und Vorrichtung zur Herstellung eines Kolbens für einen Verbrennungsmotor sowie Kolben für einen Verbrennungsmotor |
| EP3236264A3 (en) | 2009-10-13 | 2017-11-08 | Nanostring Technologies, Inc | Protein detection via nanoreporters |
| EP2542577A1 (en) | 2010-03-01 | 2013-01-09 | Lostam Biopharmaceuticals Ltd | Improved therapeutic antibodies against flagellated pseudomonas aeruginosa |
-
2010
- 2010-10-13 EP EP16201369.2A patent/EP3236264A3/en not_active Withdrawn
- 2010-10-13 JP JP2012534336A patent/JP5954876B2/ja active Active
- 2010-10-13 CA CA2775613A patent/CA2775613C/en active Active
- 2010-10-13 US US12/904,078 patent/US9714937B2/en active Active
- 2010-10-13 WO PCT/US2010/052556 patent/WO2011047087A2/en active Application Filing
- 2010-10-13 ES ES10824048.2T patent/ES2627063T3/es active Active
- 2010-10-13 AU AU2010306862A patent/AU2010306862B2/en active Active
- 2010-10-13 EP EP10824048.2A patent/EP2488876B1/en active Active
-
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- 2015-07-30 US US14/814,216 patent/US9995739B2/en active Active
-
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- 2016-04-15 JP JP2016081762A patent/JP2016136161A/ja active Pending
- 2016-04-15 JP JP2016081763A patent/JP6225212B2/ja active Active
-
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- 2018-04-24 HK HK18105349.7A patent/HK1249580A1/en unknown
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6268147B1 (en) * | 1998-11-02 | 2001-07-31 | Kenneth Loren Beattie | Nucleic acid analysis using sequence-targeted tandem hybridization |
| WO2003003810A2 (en) * | 2001-07-03 | 2003-01-16 | The Institute For Systems Biology | Methods for detection and quantification of analytes in complex mixtures |
| WO2005071401A2 (en) * | 2004-01-15 | 2005-08-04 | Chiron Corporation | Homogeneous multiplex assay for nucleic acid targets |
| WO2007076128A2 (en) * | 2005-12-23 | 2007-07-05 | Nanostring Technologies, Inc. | Nanoreporters and methods of manufacturing and use thereof |
| WO2008124847A2 (en) * | 2007-04-10 | 2008-10-16 | Nanostring Technologies, Inc. | Methods and computer systems for identifying target-specific sequences for use in nanoreporters |
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| EP3236264A2 (en) | 2017-10-25 |
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| EP2488876B1 (en) | 2017-03-01 |
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