AU2009228323B2 - Zymomonas with improved xylose utilization - Google Patents

Zymomonas with improved xylose utilization Download PDF

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Publication number
AU2009228323B2
AU2009228323B2 AU2009228323A AU2009228323A AU2009228323B2 AU 2009228323 B2 AU2009228323 B2 AU 2009228323B2 AU 2009228323 A AU2009228323 A AU 2009228323A AU 2009228323 A AU2009228323 A AU 2009228323A AU 2009228323 B2 AU2009228323 B2 AU 2009228323B2
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AU
Australia
Prior art keywords
xylose
seq
xylose isomerase
sequence
strain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU2009228323A
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English (en)
Other versions
AU2009228323A1 (en
Inventor
Perry G. Caimi
Yat-Chen Chou
Mary Ann Franden
Laura Mccole
Carol Mccutchen
Luan Tao
Paul V. Viitanen
Min Zhang
Yuying Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alliance for Sustainable Energy LLC
EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
Alliance for Sustainable Energy LLC
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Application filed by EI Du Pont de Nemours and Co, Alliance for Sustainable Energy LLC filed Critical EI Du Pont de Nemours and Co
Publication of AU2009228323A1 publication Critical patent/AU2009228323A1/en
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Publication of AU2009228323B2 publication Critical patent/AU2009228323B2/en
Ceased legal-status Critical Current
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90Isomerases (5.)
    • C12N9/92Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0008Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1022Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • C12P7/065Ethanol, i.e. non-beverage with microorganisms other than yeasts
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2009228323A 2008-03-27 2009-03-25 Zymomonas with improved xylose utilization Ceased AU2009228323B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US3987808P 2008-03-27 2008-03-27
US61/039,878 2008-03-27
PCT/US2009/038164 WO2009120731A2 (en) 2008-03-27 2009-03-25 Zymomonas with improved xylose utilization

Publications (2)

Publication Number Publication Date
AU2009228323A1 AU2009228323A1 (en) 2009-10-01
AU2009228323B2 true AU2009228323B2 (en) 2014-01-30

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
AU2009228323A Ceased AU2009228323B2 (en) 2008-03-27 2009-03-25 Zymomonas with improved xylose utilization

Country Status (8)

Country Link
US (1) US7998722B2 (enExample)
EP (1) EP2271743A2 (enExample)
JP (1) JP2011515107A (enExample)
CN (1) CN102037120B (enExample)
AU (1) AU2009228323B2 (enExample)
BR (1) BRPI0906333A2 (enExample)
CA (1) CA2716585C (enExample)
WO (1) WO2009120731A2 (enExample)

Families Citing this family (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0822937D0 (en) 2008-12-16 2009-01-21 Terranol As Microorganism
US8247208B2 (en) 2008-12-22 2012-08-21 Alliance For Sustainable Energy Llc Zymomonas with improved xylose utilization in stress conditions
US20110143408A1 (en) * 2009-06-18 2011-06-16 E. I. Du Pont De Nemours And Company Zymomonas with improved arabinose utilization
US8647850B2 (en) 2009-12-23 2014-02-11 E I Du Pont De Nemours And Company Process for simultaneous saccharification and fermentation for production of ethanol
US10138499B2 (en) 2009-12-23 2018-11-27 Danisco Us Inc. Methods for improving the efficiency of simultaneous saccharification and fermentation reactions
US20110195448A1 (en) * 2009-12-28 2011-08-11 James Casey Lippmeier Recombinant Thraustochytrids that Grow on Xylose, and Compositions, Methods of Making, and Uses Thereof
US8691526B2 (en) 2010-01-20 2014-04-08 Xyleco, Inc. Processing materials
MX2012008409A (es) * 2010-01-20 2012-12-05 Xyleco Inc Metodo y sistema para la sacarificacion y fermentacion de una materia prima de masa biologica.
US8721794B2 (en) 2010-04-28 2014-05-13 E I Du Pont De Nemours And Company Production of high solids syrup from lignocellulosic biomass hydrolysate fermentation broth
US8906235B2 (en) 2010-04-28 2014-12-09 E I Du Pont De Nemours And Company Process for liquid/solid separation of lignocellulosic biomass hydrolysate fermentation broth
NZ603291A (en) 2010-06-18 2015-01-30 Butamax Tm Advanced Biofuels Extraction solvents derived from oil for alcohol removal in extractive fermentation
KR20130116858A (ko) 2010-06-18 2013-10-24 부타맥스 어드밴스드 바이오퓨얼스 엘엘씨 추출 발효에서 알코올 수거를 위한 오일 유래의 추출 용매
US9040263B2 (en) 2010-07-28 2015-05-26 Butamax Advanced Biofuels Llc Production of alcohol esters and in situ product removal during alcohol fermentation
US8623623B2 (en) * 2010-06-29 2014-01-07 E I Du Pont De Nemours And Company Xylose utilization in recombinant Zymomonas
US8679822B2 (en) * 2010-06-29 2014-03-25 E I Du Pont De Nemours And Company Xylose utilization in recombinant zymomonas
US8651403B2 (en) 2010-07-21 2014-02-18 E I Du Pont De Nemours And Company Anhydrous ammonia treatment for improved milling of biomass
US20120125548A1 (en) 2010-11-23 2012-05-24 E. I. Du Pont De Nemours And Company Continuously fed biomass pretreatment process for a packed bed reactor
US20120125551A1 (en) 2010-11-23 2012-05-24 E. I. Du Pont De Nemours And Company Biomass pretreatment process for a packed bed reactor
US8476048B2 (en) 2010-12-17 2013-07-02 E I Du Pont De Nemours And Company Xylose utilizing zymomonas mobilis with improved ethanol production in biomass hydrolysate medium
US20130040340A1 (en) 2011-02-07 2013-02-14 E. I. Du Pont De Nemours And Company Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms
US8759044B2 (en) 2011-03-23 2014-06-24 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
US8765425B2 (en) 2011-03-23 2014-07-01 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
US8765426B2 (en) 2011-04-07 2014-07-01 E I Du Pont De Nemours And Company Pantothenic acid biosynthesis in zymomonas
CA2837722A1 (en) 2011-06-17 2012-12-20 Butamax Advanced Biofuels Llc Co-products from biofuel production processes and methods of making
EP2554668A1 (en) * 2011-08-04 2013-02-06 DSM IP Assets B.V. A pentose sugar fermenting cell
AU2012347687A1 (en) 2011-12-09 2014-05-29 Butamax Advanced Biofuels Llc Process to remove product alcohols from fermentation broth by multistep flash evaporation
US20130157332A1 (en) * 2011-12-20 2013-06-20 E I Du Pont De Nemours And Company Gene inactivation allowing immediate growth on xylose medium by engineered zymomonas
US8911983B2 (en) 2011-12-20 2014-12-16 E I Du Pont De Nemours And Company Pnp gene modification for improved xylose utilization in Zymomonas
EP2803722B1 (en) * 2012-01-10 2017-09-27 Cj Cheiljedang Corporation Microorganisms of corynebacterium which can utilize xylose and method for producing l-lysine using same
CA2870567A1 (en) 2012-05-04 2013-11-07 Butamax Advanced Biofuels Llc Processes and systems for alcohol production and recovery
US8697426B2 (en) 2012-06-15 2014-04-15 E I Du Pont De Nemours And Company Contaminant control in Zymomonas fermentation using virginiamycin
US8759069B2 (en) 2012-06-15 2014-06-24 E I Du Pont De Nemours And Company Contaminant control in Zymomonas fermentation using hop acids
US20140024064A1 (en) 2012-07-23 2014-01-23 Butamax(Tm) Advanced Biofuels Llc Processes and systems for the production of fermentative alcohols
BR112015002621A2 (pt) 2012-08-06 2018-05-22 Danisco Us Inc células fúngicas que produzem quantidades reduzidas de peptaibols
NZ705079A (en) 2012-09-12 2017-10-27 Butamax Advanced Biofuels Llc Processes and systems for the production of fermentation products
BR112015006296A2 (pt) 2012-09-21 2017-07-04 Butamax Advanced Biofuels Llc ''processo, composição bifásica, métodos para a manutenção do estado oxidativo de uma composição, para otimizar a extração do butanol, para aprimorar o rendimento de uma fermetanção de isobutanol, para aprimorar o título de isobutanol produzido durante a fermentação e para aprimorar a taxa de produção de isobutanol durante uma fermentação''
JP6151785B2 (ja) * 2012-10-10 2017-06-21 バイオコン・リミテッド ヌクレオチド配列およびその製造
CA2887574A1 (en) 2012-10-11 2014-04-17 Butamax Advanced Biofuels Llc Processes and systems for the production of fermentation products
US20140142352A1 (en) 2012-11-20 2014-05-22 Butamax Advanced Biofuels Llc Butanol purification
US20140178954A1 (en) * 2012-12-20 2014-06-26 E I Du Pont De Nemours And Company Expression of xylose isomerase activity in yeast
US8846357B2 (en) 2012-12-20 2014-09-30 E I Du Pont De Nemours And Company Stabilized chlorine dioxide for contamination control in Zymomonas fermentation
WO2014130352A1 (en) 2013-02-21 2014-08-28 Butamax Advanced Biofuels Llc Recovery of thermal energy as heat source in the production of butanol from a fermentation process
US9523104B2 (en) 2013-03-12 2016-12-20 Butamax Advanced Biofuels Llc Processes and systems for the production of alcohols
US20140273105A1 (en) 2013-03-12 2014-09-18 E I Du Pont De Nemours And Company Gradient pretreatment of lignocellulosic biomass
BR112015022612A2 (pt) 2013-03-14 2017-10-24 Du Pont microrganismo recombinante e enzima
WO2014144728A1 (en) 2013-03-15 2014-09-18 Butamax Advanced Biofuels Llc Method for production of butanol using extractive fermentation
WO2014144643A1 (en) 2013-03-15 2014-09-18 Butamax Advanced Biofuels Llc Method for producing butanol using extractive fermentation
WO2014151645A1 (en) 2013-03-15 2014-09-25 Butamax Advanced Biofuels Llc Process for maximizing biomass growth and butanol yield by feedback control
DE102013205986A1 (de) 2013-04-04 2014-10-23 Wacker Chemie Ag Mikroorganismenstamm und Verfahren zur fermentativen Herstellung von C4-Verbindungen aus C5-Zuckern
US9663759B2 (en) 2013-07-03 2017-05-30 Butamax Advanced Biofuels Llc Partial adaptation for butanol production
US20150087040A1 (en) 2013-09-26 2015-03-26 E I Du Pont De Nemours And Company Production of ethanol and recycle water in a cellulosic fermentation process
US9139818B2 (en) 2013-09-26 2015-09-22 E I Du Pont De Nemours And Company High expression Zymomonas promoters
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WO2015057517A1 (en) 2013-10-17 2015-04-23 Danisco Us Inc. Use of hemicellulases to improve ethanol production
US10227623B2 (en) 2013-11-24 2019-03-12 E I Du Pont De Nemours And Company High force and high stress destructuring of cellulosic biomass
US9371546B2 (en) 2014-06-24 2016-06-21 E I Du Pont De Nemours And Company Enhancing D-xylose and L-arabinose utilization in Zymomonas cells
JP6977231B2 (ja) 2015-07-13 2021-12-08 マラ リニューアブルズ コーポレーション C5有機炭素の微生物による代謝の増強
BR112018013214A2 (pt) 2015-12-28 2018-12-11 Danisco Us Inc métodos para a produção de produtos de fermentação a partir de matéria-prima
US11091782B2 (en) * 2017-10-27 2021-08-17 Alliance For Sustainable Energy, Llc Engineered zymomonas for the production of 2,3-butanediol
KR20200135469A (ko) * 2018-03-27 2020-12-02 바스프 에스이 자일로스 대사 효모
CN112912489A (zh) * 2018-10-23 2021-06-04 玛拉可再生能源公司 微生物在半纤维素衍生碳水化合物中生长的适应和工艺优化
BR112022004257A2 (pt) * 2019-09-13 2022-07-12 Danisco Us Inc Variantes de glicose isomerase termoestáveis
WO2023110822A1 (en) * 2021-12-14 2023-06-22 C-Lecta Gmbh Glucose isomerases
WO2025029677A2 (en) * 2023-07-28 2025-02-06 Bp Corporation North America Inc. Enzymes for fructose isomerization
CN120249263B (zh) * 2025-04-02 2025-12-09 湖北大学 木糖异构酶突变体、其核酸分子和表达载体及应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004037973A2 (en) * 2002-04-27 2004-05-06 Midwest Research Institute Zymomonas pentose-sugar fermentating strains and uses thereof
WO2008133638A2 (en) * 2006-09-28 2008-11-06 E. I. Du Pont De Nemours And Company Xylitol synthesis mutant of xylose-utilizing zymomonas for ethanol production

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5231020A (en) * 1989-03-30 1993-07-27 Dna Plant Technology Corporation Genetic engineering of novel plant phenotypes
WO1995028476A1 (en) 1994-04-15 1995-10-26 Midwest Research Institute Recombinant zymomonas for pentose fermentation
US5514583A (en) * 1994-04-15 1996-05-07 Midwest Research Institute Recombinant zymomonas for pentose fermentation
US5712133A (en) * 1994-04-15 1998-01-27 Midwest Research Institute Pentose fermentation by recombinant zymomonas
AU2001249926B2 (en) * 2000-05-01 2006-07-20 Alliance For Sustainable Energy, Llc Stable zymomonas mobilis xylose and arabinose fermenting strains
US6566107B1 (en) * 2000-11-01 2003-05-20 Midwest Research Institute Recombinant Zymomonas mobilis with improved xylose utilization
US20040231060A1 (en) 2003-03-07 2004-11-25 Athenix Corporation Methods to enhance the activity of lignocellulose-degrading enzymes
BRPI0619325B8 (pt) * 2005-10-26 2020-11-17 Butamax Advanced Biofuels Llc célula hospedeira microbiana recombinante e método para produção de isobutanol
US7629156B2 (en) * 2006-09-28 2009-12-08 E.I. Du Pont De Nemours And Company Ethanol production in fermentation of mixed sugars containing xylose
US7741084B2 (en) * 2006-09-28 2010-06-22 E. I. Du Pont De Nemours And Company Ethanol production using xylitol synthesis mutant of xylose-utilizing zymomonas

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004037973A2 (en) * 2002-04-27 2004-05-06 Midwest Research Institute Zymomonas pentose-sugar fermentating strains and uses thereof
WO2008133638A2 (en) * 2006-09-28 2008-11-06 E. I. Du Pont De Nemours And Company Xylitol synthesis mutant of xylose-utilizing zymomonas for ethanol production

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
FELDMANN, S. D. et al., Appl. Microbiol. Biotechnol., 1992, Vol. 38, pages 354-361 & FELDMANN, S. D. et al., Mol. Gen. Genet., 1992, Vol. 234, No. 2, pages 201-210 *

Also Published As

Publication number Publication date
CN102037120A (zh) 2011-04-27
US7998722B2 (en) 2011-08-16
WO2009120731A3 (en) 2009-11-12
BRPI0906333A2 (pt) 2017-05-23
WO2009120731A2 (en) 2009-10-01
CA2716585C (en) 2017-05-09
JP2011515107A (ja) 2011-05-19
EP2271743A2 (en) 2011-01-12
AU2009228323A1 (en) 2009-10-01
CN102037120B (zh) 2013-09-11
CA2716585A1 (en) 2009-10-01
US20090246846A1 (en) 2009-10-01

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