AU2008310955B2 - Methods, compositions and kits for the improved detection of small RNA molecules - Google Patents

Methods, compositions and kits for the improved detection of small RNA molecules Download PDF

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Publication number
AU2008310955B2
AU2008310955B2 AU2008310955A AU2008310955A AU2008310955B2 AU 2008310955 B2 AU2008310955 B2 AU 2008310955B2 AU 2008310955 A AU2008310955 A AU 2008310955A AU 2008310955 A AU2008310955 A AU 2008310955A AU 2008310955 B2 AU2008310955 B2 AU 2008310955B2
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Australia
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modified
duplex
base
amplification
target sequence
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AU2008310955A
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AU2008310955A1 (en
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Igor Vassily Kutyavin
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/117—Modifications characterised by incorporating modified base
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/207—Modifications characterised by siRNA, miRNA

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2008310955A 2007-10-09 2008-10-08 Methods, compositions and kits for the improved detection of small RNA molecules Ceased AU2008310955B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US97865707P 2007-10-09 2007-10-09
US60/978,657 2007-10-09
PCT/US2008/079169 WO2009048928A1 (en) 2007-10-09 2008-10-08 Methods, compositions and kits for the improved detection of small rna molecules

Publications (2)

Publication Number Publication Date
AU2008310955A1 AU2008310955A1 (en) 2009-04-16
AU2008310955B2 true AU2008310955B2 (en) 2015-01-29

Family

ID=40242697

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2008310955A Ceased AU2008310955B2 (en) 2007-10-09 2008-10-08 Methods, compositions and kits for the improved detection of small RNA molecules

Country Status (7)

Country Link
US (2) US8637276B2 (https=)
EP (1) EP2198059B1 (https=)
JP (1) JP5486501B2 (https=)
AU (1) AU2008310955B2 (https=)
CA (1) CA2702584A1 (https=)
ES (1) ES2526925T3 (https=)
WO (1) WO2009048928A1 (https=)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2198059B1 (en) * 2007-10-09 2014-12-03 Igor Vassily Kutyavin Methods for the improved detection of small rna molecules
US9074248B1 (en) * 2012-12-18 2015-07-07 Qiagen Gmbh Primers for helicase dependent amplification and their methods of use
WO2016129941A1 (ko) * 2015-02-09 2016-08-18 주식회사 하임바이오텍 마이크로알앤에이 검출용 키트 및 방법
WO2017087943A1 (en) * 2015-11-20 2017-05-26 Brandeis University Amplifying and detecting coconut cadang-cadang viroid rna
US10913986B2 (en) 2016-02-01 2021-02-09 The Board Of Regents Of The University Of Nebraska Method of identifying important methylome features and use thereof
JP6970732B2 (ja) * 2016-07-21 2021-11-24 ハイムバイオテック インコーポレイテッド Ｒｎａ検出用キット及び方法
JP7055691B2 (ja) 2017-07-11 2022-04-18 株式会社東芝 短鎖核酸伸長用プライマーセット、アッセイキット、短鎖核酸伸長方法、増幅方法及び検出方法
ES3030914T3 (en) * 2019-11-04 2025-07-02 Mirnax Biosens S L Bivalent reverse primer
EP3901286A1 (en) * 2020-04-24 2021-10-27 Mirnax Biosens, S.L. Bivalent reverse primer
CN118166080B (zh) * 2024-04-15 2025-04-04 苏州市立医院 一种无RNA逆转录酶的miRNA一步法检测方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005098029A2 (en) * 2004-04-07 2005-10-20 Exiqon A/S Methods for quantification of micrornas and small interfering rnas
WO2007127992A2 (en) * 2006-04-28 2007-11-08 Igor Kutyavin Use of base-modified deoxynucleoside triphosphates

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6218118B1 (en) * 1998-07-09 2001-04-17 Agilent Technologies, Inc. Method and mixture reagents for analyzing the nucleotide sequence of nucleic acids by mass spectrometry
EP1261616B1 (en) * 2000-03-01 2010-08-18 Epoch Biosciences, Inc. Modified oligonucleotides for mismatch discrimination
EP2198059B1 (en) * 2007-10-09 2014-12-03 Igor Vassily Kutyavin Methods for the improved detection of small rna molecules

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005098029A2 (en) * 2004-04-07 2005-10-20 Exiqon A/S Methods for quantification of micrornas and small interfering rnas
WO2007127992A2 (en) * 2006-04-28 2007-11-08 Igor Kutyavin Use of base-modified deoxynucleoside triphosphates

Also Published As

Publication number Publication date
US20100304442A1 (en) 2010-12-02
EP2198059B1 (en) 2014-12-03
WO2009048928A1 (en) 2009-04-16
JP2011501663A (ja) 2011-01-13
US8980558B2 (en) 2015-03-17
US20140170666A1 (en) 2014-06-19
JP5486501B2 (ja) 2014-05-07
AU2008310955A1 (en) 2009-04-16
EP2198059A1 (en) 2010-06-23
US8637276B2 (en) 2014-01-28
ES2526925T3 (es) 2015-01-16
CA2702584A1 (en) 2009-04-16

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