AU2003202817A1 - Manufacturing method of recombinant protein in yeast by the use of secretory type vector - Google Patents

Manufacturing method of recombinant protein in yeast by the use of secretory type vector

Info

Publication number
AU2003202817A1
AU2003202817A1 AU2003202817A AU2003202817A AU2003202817A1 AU 2003202817 A1 AU2003202817 A1 AU 2003202817A1 AU 2003202817 A AU2003202817 A AU 2003202817A AU 2003202817 A AU2003202817 A AU 2003202817A AU 2003202817 A1 AU2003202817 A1 AU 2003202817A1
Authority
AU
Australia
Prior art keywords
yeast
manufacturing
recombinant protein
type vector
secretory type
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2003202817A
Inventor
Cheolho Jung
Hyung-Cheol Kim
Kangmoon Lee
Seung-Joo Lee
Seungwon Lee
Young-Pil Lee
Dae-Bong Moon
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
LG Chem Ltd
Original Assignee
LG Life Sciences Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by LG Life Sciences Ltd filed Critical LG Life Sciences Ltd
Publication of AU2003202817A1 publication Critical patent/AU2003202817A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2820/00—Vectors comprising a special origin of replication system
    • C12N2820/70—Vectors comprising a special origin of replication system from fungi
    • C12N2820/702—Vectors comprising a special origin of replication system from fungi yeast

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2003202817A 2002-01-21 2003-01-13 Manufacturing method of recombinant protein in yeast by the use of secretory type vector Abandoned AU2003202817A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR10-2002-0003289 2002-01-21
KR1020020003289A KR20030062854A (en) 2002-01-21 2002-01-21 Manufacturing method of recombinant protein in yeast by the use of secretory type vector
PCT/KR2003/000061 WO2003060141A1 (en) 2002-01-21 2003-01-13 Manufacturing method of recombinant protein in yeast by the use of secretory type vector

Publications (1)

Publication Number Publication Date
AU2003202817A1 true AU2003202817A1 (en) 2003-07-30

Family

ID=19718661

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2003202817A Abandoned AU2003202817A1 (en) 2002-01-21 2003-01-13 Manufacturing method of recombinant protein in yeast by the use of secretory type vector

Country Status (3)

Country Link
KR (1) KR20030062854A (en)
AU (1) AU2003202817A1 (en)
WO (1) WO2003060141A1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100620495B1 (en) * 2004-08-02 2006-09-08 학교법인 동의학원 Process for preparing xylooigosaccharide using recombinant endoxylanase
KR100726716B1 (en) * 2006-08-14 2007-06-11 주식회사 인 바이오넷 A New Strain Highly Producing PDGF-BB and a Secretive Protein for the Strain
KR100726143B1 (en) * 2006-08-14 2007-06-13 주식회사 인 바이오넷 - A New Strain Highly Producing PDGF-BB and a Method for Constructing the Strain
KR100726717B1 (en) * 2006-08-14 2007-06-11 주식회사 인 바이오넷 - A New Strain Highly Producing PDGF-BB and a Secretive Protein GST for the Strain
WO2008020701A1 (en) * 2006-08-14 2008-02-21 In Bionet Co., Ltd A new strain highly producing pdgf-bb
KR102092225B1 (en) 2014-04-30 2020-03-23 주식회사 엘지화학 A protein secretory factor with a high secretory efficiency and a expression vector comprising the same
CN107460137A (en) * 2016-06-03 2017-12-12 中国科学院青岛生物能源与过程研究所 A kind of saccharomyces cerevisiae of fermentable inulin production FOS and its construction method and application
JP7237365B2 (en) 2017-03-10 2023-03-13 ボルト スレッズ インコーポレイテッド Compositions and methods for producing high secretory yields of recombinant proteins
KR102486713B1 (en) * 2017-03-10 2023-01-10 볼트 쓰레즈, 인크. Compositions and methods for producing recombinant proteins in high secretion yield

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4810643A (en) * 1985-08-23 1989-03-07 Kirin- Amgen Inc. Production of pluripotent granulocyte colony-stimulating factor
AU686840B2 (en) * 1994-07-07 1998-02-12 Immunex Corporation Fusion proteins comprising GM-CSF and antigens and their expression in yeast
KR0180081B1 (en) * 1995-10-26 1999-04-01 김용구 Preparation process of gcsf from yeast
KR20020066482A (en) * 2001-02-12 2002-08-19 프로테온 주식회사 Secretion system of recombinant protein containing sequence enhancer and the promoter of methylotropic yeast

Also Published As

Publication number Publication date
WO2003060141A1 (en) 2003-07-24
KR20030062854A (en) 2003-07-28

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase