AU2002344527A1 - The feeder cell layer for in vitro culturing human embryonic stem cells and the method for culturing embryonic stem cells - Google Patents

The feeder cell layer for in vitro culturing human embryonic stem cells and the method for culturing embryonic stem cells

Info

Publication number
AU2002344527A1
AU2002344527A1 AU2002344527A AU2002344527A AU2002344527A1 AU 2002344527 A1 AU2002344527 A1 AU 2002344527A1 AU 2002344527 A AU2002344527 A AU 2002344527A AU 2002344527 A AU2002344527 A AU 2002344527A AU 2002344527 A1 AU2002344527 A1 AU 2002344527A1
Authority
AU
Australia
Prior art keywords
stem cells
embryonic stem
culturing
cell layer
feeder cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002344527A
Other languages
English (en)
Inventor
Ge Lin
Guangxiu Lu
Changqing Xie
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hunan Hui-Lin Life Technology Co
Original Assignee
CHANGSHA HUILIN STEM CELL ENGI
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CHANGSHA HUILIN STEM CELL ENGI filed Critical CHANGSHA HUILIN STEM CELL ENGI
Publication of AU2002344527A1 publication Critical patent/AU2002344527A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts
AU2002344527A 2002-10-25 2002-10-25 The feeder cell layer for in vitro culturing human embryonic stem cells and the method for culturing embryonic stem cells Abandoned AU2002344527A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2002/000753 WO2004038012A1 (fr) 2002-10-25 2002-10-25 Couche de cellules nourricieres pour la culture in vitro de cellules souches embryonnaires humaines et methode de culture de cellules souches embryonnaires

Publications (1)

Publication Number Publication Date
AU2002344527A1 true AU2002344527A1 (en) 2004-05-13

Family

ID=32111536

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002344527A Abandoned AU2002344527A1 (en) 2002-10-25 2002-10-25 The feeder cell layer for in vitro culturing human embryonic stem cells and the method for culturing embryonic stem cells

Country Status (3)

Country Link
CN (1) CN1717478A (zh)
AU (1) AU2002344527A1 (zh)
WO (1) WO2004038012A1 (zh)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110669726A (zh) * 2019-09-19 2020-01-10 上海长征医院 一种人皮肤成纤维细胞伽马射线辐照后可长期传代细胞株及其构建方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843780A (en) * 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
AU3881499A (en) * 1998-09-01 2000-03-21 Wisconsin Alumni Research Foundation Primate embryonic stem cells with compatible histocompatibility genes
US7005252B1 (en) * 2000-03-09 2006-02-28 Wisconsin Alumni Research Foundation Serum free cultivation of primate embryonic stem cells

Also Published As

Publication number Publication date
CN1717478A (zh) 2006-01-04
WO2004038012A1 (fr) 2004-05-06

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase