AU2001233710A1 - Method for the rapid detection of whole microorganisms on retaining membranes byuse of chaotropic agents - Google Patents
Method for the rapid detection of whole microorganisms on retaining membranes byuse of chaotropic agentsInfo
- Publication number
- AU2001233710A1 AU2001233710A1 AU2001233710A AU3371001A AU2001233710A1 AU 2001233710 A1 AU2001233710 A1 AU 2001233710A1 AU 2001233710 A AU2001233710 A AU 2001233710A AU 3371001 A AU3371001 A AU 3371001A AU 2001233710 A1 AU2001233710 A1 AU 2001233710A1
- Authority
- AU
- Australia
- Prior art keywords
- byuse
- rapid detection
- chaotropic agents
- retaining membranes
- whole microorganisms
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 239000012528 membrane Substances 0.000 title abstract 3
- 230000003196 chaotropic effect Effects 0.000 title abstract 2
- 238000001514 detection method Methods 0.000 title abstract 2
- 244000005700 microbiome Species 0.000 title abstract 2
- 238000000034 method Methods 0.000 title 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 abstract 3
- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical compound NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 abstract 2
- 239000012530 fluid Substances 0.000 abstract 2
- UMGDCJDMYOKAJW-UHFFFAOYSA-N thiourea Chemical compound NC(N)=S UMGDCJDMYOKAJW-UHFFFAOYSA-N 0.000 abstract 2
- CHJJGSNFBQVOTG-UHFFFAOYSA-N N-methyl-guanidine Natural products CNC(N)=N CHJJGSNFBQVOTG-UHFFFAOYSA-N 0.000 abstract 1
- 206010036790 Productive cough Diseases 0.000 abstract 1
- 230000000890 antigenic effect Effects 0.000 abstract 1
- 210000001124 body fluid Anatomy 0.000 abstract 1
- 239000010839 body fluid Substances 0.000 abstract 1
- 239000004202 carbamide Substances 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- SWSQBOPZIKWTGO-UHFFFAOYSA-N dimethylaminoamidine Natural products CN(C)C(N)=N SWSQBOPZIKWTGO-UHFFFAOYSA-N 0.000 abstract 1
- 150000002540 isothiocyanates Chemical class 0.000 abstract 1
- 230000000717 retained effect Effects 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 abstract 1
- 210000003802 sputum Anatomy 0.000 abstract 1
- 208000024794 sputum Diseases 0.000 abstract 1
- 210000002700 urine Anatomy 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
Abstract
The detection of microorganisms concentrated from body fluids as serum, sputum, pericardiac fluid, urine or other fluid on the surface of retaining membranes by specific partners of reaction that bind to antigenic components of the retained organisms is facilitated when the organisms concentrated on the membrane are treated with a high molar concentration of a chaotropic agent as guanidine, urea, isothiocyanate, thiourea.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP00870013A EP1122542A1 (en) | 2000-02-01 | 2000-02-01 | Method for the rapid detection of whole microorganisms on retaining membranes by use of chaotropic agents |
EP00870013 | 2000-02-01 | ||
PCT/EP2001/000829 WO2001057532A1 (en) | 2000-02-01 | 2001-01-26 | Method for the rapid detection of whole microorganisms on retaining membranes by use of chaotropic agents |
Publications (1)
Publication Number | Publication Date |
---|---|
AU2001233710A1 true AU2001233710A1 (en) | 2001-08-14 |
Family
ID=8175698
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU2001233710A Abandoned AU2001233710A1 (en) | 2000-02-01 | 2001-01-26 | Method for the rapid detection of whole microorganisms on retaining membranes byuse of chaotropic agents |
Country Status (6)
Country | Link |
---|---|
US (1) | US6846648B2 (en) |
EP (2) | EP1122542A1 (en) |
AT (1) | ATE294393T1 (en) |
AU (1) | AU2001233710A1 (en) |
DE (1) | DE60110369T2 (en) |
WO (1) | WO2001057532A1 (en) |
Families Citing this family (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB0304832D0 (en) * | 2003-03-04 | 2003-04-09 | Secr Defence | Assay method |
US20060046305A1 (en) * | 2003-10-15 | 2006-03-02 | National University Of Singapore | Method and apparatus for detecting analyte with filter |
GB2409519B (en) * | 2003-12-22 | 2005-11-09 | Prail Price Richardson Diagnos | Microorganism detector |
EP2021018A4 (en) * | 2006-05-18 | 2009-06-03 | Daniel G Ericson | Rapid detection of mycobacterium tuberculosis and antimicrobial drug resistance |
US20090181078A1 (en) * | 2006-09-26 | 2009-07-16 | Infectious Disease Research Institute | Vaccine composition containing synthetic adjuvant |
DK2468300T3 (en) | 2006-09-26 | 2018-01-29 | Infectious Disease Res Inst | Vaccine composition containing synthetic adjuvant |
CN102481312B (en) | 2009-06-05 | 2015-07-15 | 传染性疾病研究院 | Synthetic Glucopyranosyl Lipid Adjuvants |
CN103596586A (en) | 2011-04-08 | 2014-02-19 | 免疫设计公司 | Immunogenic compositions and methods of using the compositions for inducing humoral and cellular immune responses |
HUE044841T2 (en) | 2012-02-07 | 2019-11-28 | Infectious Disease Res Inst | Improved adjuvant formulations comprising tlr4 agonists and methods of using the same |
ES2787455T3 (en) | 2012-05-16 | 2020-10-16 | Immune Design Corp | HSV-2 vaccines |
JP6091158B2 (en) * | 2012-10-23 | 2017-03-08 | デンカ生研株式会社 | Method to increase sensitivity of immunoassay system by pretreatment of urine with denaturant |
AU2014253791B2 (en) | 2013-04-18 | 2019-05-02 | Immune Design Corp. | GLA monotherapy for use in cancer treatment |
US9463198B2 (en) | 2013-06-04 | 2016-10-11 | Infectious Disease Research Institute | Compositions and methods for reducing or preventing metastasis |
AU2014373928C1 (en) | 2013-12-31 | 2020-12-17 | Access To Advanced Health Institute | Single vial vaccine formulations |
CN105759038A (en) * | 2016-04-15 | 2016-07-13 | 肖乐义 | Immunoassay method and kit for assaying mycobacterium tuberculosis from biological samples |
CN105717310A (en) * | 2016-04-15 | 2016-06-29 | 肖乐义 | Immuno-fluorescent staining method for detecting mycobacterium tuberculosis in leukocytes and kit |
MX2021010105A (en) | 2016-06-01 | 2022-06-16 | Infectious Disease Res Inst | Nanoalum particles containing a sizing agent. |
GB201703383D0 (en) | 2017-03-02 | 2017-04-19 | Gargle Tech Ltd | Testing for particulates |
JP7454264B2 (en) | 2018-09-05 | 2024-03-22 | ヒーロー サイエンティフィック リミテッド | particle inspection |
MX2021014363A (en) | 2019-05-25 | 2022-02-21 | Infectious Disease Res Inst | Composition and method for spray drying an adjuvant vaccine emulsion. |
EP4165385B1 (en) | 2021-01-06 | 2024-04-17 | Hero Scientific Ltd | Filtration sampling devices |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5482834A (en) * | 1982-05-17 | 1996-01-09 | Hahnemann University | Evaluation of nucleic acids in a biological sample hybridization in a solution of chaotrophic salt solubilized cells |
US4695537A (en) * | 1982-05-21 | 1987-09-22 | The University Of Tennessee Research Corp. | Particles sensitized with detergent-treated antigen for agglutination immunoassay |
US4808518A (en) * | 1985-02-11 | 1989-02-28 | University Of Tennessee Research Corporation | Recovery of cytomegalovirus antigen and use thereof in an assay |
CA1278259C (en) * | 1986-02-26 | 1990-12-27 | William C. Saxinger | Competitive elisa for the detection of antibodies |
US4959303A (en) * | 1987-12-21 | 1990-09-25 | Syntex (U.S.A.) Inc. | Assay for antigens by binding immune complexes to solid supports free of protein and non-ionic binders |
US4978613A (en) * | 1989-01-17 | 1990-12-18 | Abbott Laboratories | Beta-lactamase assay employing chromogenic precipitating substrates |
US5155023A (en) * | 1990-02-09 | 1992-10-13 | Synbiotics Corporation | Enzyme immunoassay procedure for amphipathic analytes |
US5420017A (en) * | 1991-01-24 | 1995-05-30 | Orion Corporation Ltd. | Method and kit for the detection of microorganisms |
-
2000
- 2000-02-01 EP EP00870013A patent/EP1122542A1/en not_active Withdrawn
-
2001
- 2001-01-26 AU AU2001233710A patent/AU2001233710A1/en not_active Abandoned
- 2001-01-26 WO PCT/EP2001/000829 patent/WO2001057532A1/en active IP Right Grant
- 2001-01-26 AT AT01905689T patent/ATE294393T1/en not_active IP Right Cessation
- 2001-01-26 EP EP01905689A patent/EP1252518B1/en not_active Expired - Lifetime
- 2001-01-26 DE DE60110369T patent/DE60110369T2/en not_active Expired - Fee Related
- 2001-01-26 US US10/182,793 patent/US6846648B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
US20030022160A1 (en) | 2003-01-30 |
ATE294393T1 (en) | 2005-05-15 |
EP1122542A1 (en) | 2001-08-08 |
EP1252518A1 (en) | 2002-10-30 |
US6846648B2 (en) | 2005-01-25 |
DE60110369D1 (en) | 2005-06-02 |
DE60110369T2 (en) | 2006-03-09 |
WO2001057532A1 (en) | 2001-08-09 |
EP1252518B1 (en) | 2005-04-27 |
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