ATE552339T1 - Signalverstärkungsverfahren zum nachweis eines mutanten gens - Google Patents
Signalverstärkungsverfahren zum nachweis eines mutanten gensInfo
- Publication number
- ATE552339T1 ATE552339T1 AT04713241T AT04713241T ATE552339T1 AT E552339 T1 ATE552339 T1 AT E552339T1 AT 04713241 T AT04713241 T AT 04713241T AT 04713241 T AT04713241 T AT 04713241T AT E552339 T1 ATE552339 T1 AT E552339T1
- Authority
- AT
- Austria
- Prior art keywords
- signal amplification
- detecting
- amplification method
- mutant gene
- detection sensitivity
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2003044859 | 2003-02-21 | ||
PCT/JP2004/002007 WO2004074480A1 (ja) | 2003-02-21 | 2004-02-20 | 変異遺伝子検出のためのシグナル増幅方法 |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE552339T1 true ATE552339T1 (de) | 2012-04-15 |
Family
ID=32905470
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT04713241T ATE552339T1 (de) | 2003-02-21 | 2004-02-20 | Signalverstärkungsverfahren zum nachweis eines mutanten gens |
Country Status (9)
Country | Link |
---|---|
US (1) | US7632639B2 (de) |
EP (1) | EP1595953B1 (de) |
JP (1) | JP4255472B2 (de) |
KR (1) | KR101111568B1 (de) |
CN (1) | CN100532550C (de) |
AT (1) | ATE552339T1 (de) |
AU (1) | AU2004213716B2 (de) |
CA (1) | CA2516360C (de) |
WO (1) | WO2004074480A1 (de) |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7862998B2 (en) | 2005-02-28 | 2011-01-04 | Eisai R&D Management Co., Ltd. | Assist probe and method of using the same |
JP4616881B2 (ja) * | 2005-02-28 | 2011-01-19 | エーザイ・アール・アンド・ディー・マネジメント株式会社 | アシストプローブ及びその利用方法 |
JP4847961B2 (ja) * | 2005-09-27 | 2011-12-28 | エーザイ・アール・アンド・ディー・マネジメント株式会社 | 微小粒子に自己集合体を形成させる方法及び標的分析物の検出方法 |
US7927804B2 (en) | 2006-03-15 | 2011-04-19 | Eisai & Managment Co., Ltd. | Method of forming signal probe-polymer |
EP2180062B1 (de) * | 2007-08-14 | 2012-10-03 | Eisai R&D Management Co., Ltd. | Verfahren zur erkennung einer zielsubstanz |
WO2009022682A1 (ja) | 2007-08-14 | 2009-02-19 | Eisai R & D Management Co., Ltd. | 標的物質の検出方法 |
EE201000013A (et) | 2010-01-29 | 2011-10-17 | Selfdiagnostics O� | Meetod ja kiirtesti seade sihtmrk-molekuli proovimaterjalist detekteerimiseks |
CN102304565B (zh) * | 2011-04-29 | 2014-03-05 | 益善生物技术股份有限公司 | 一种hfe基因多态性检测特异性引物和液相芯片 |
JPWO2013085026A1 (ja) | 2011-12-09 | 2015-04-27 | エーザイ・アール・アンド・ディー・マネジメント株式会社 | 塩基変異の検出方法及び検出用キット |
JP6896620B2 (ja) * | 2014-10-14 | 2021-06-30 | アボット・ラボラトリーズAbbott Laboratories | ロックされた核酸を有する配列変換およびシグナル増幅dnaならびにそれを用いた検出方法 |
CN108277260A (zh) * | 2018-04-16 | 2018-07-13 | 吉林大学 | 一种检测brafv600e基因突变的方法 |
JP7161173B2 (ja) * | 2018-06-29 | 2022-10-26 | 積水メディカル株式会社 | siRNAの定量方法 |
JP6718032B1 (ja) * | 2018-09-11 | 2020-07-08 | 積水メディカル株式会社 | オリゴヌクレオチドの検出、または定量方法 |
WO2021095829A1 (ja) * | 2019-11-14 | 2021-05-20 | 積水メディカル株式会社 | オリゴヌクレオチドの検出又は定量方法 |
KR20210109745A (ko) * | 2020-02-28 | 2021-09-07 | 주식회사 누리바이오 | 단일 표적 유전자의 유전적 변이 실시간 검출용 단일핵산 및 이를 이용한 검출 방법 |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4988617A (en) | 1988-03-25 | 1991-01-29 | California Institute Of Technology | Method of detecting a nucleotide change in nucleic acids |
DK51092D0 (da) | 1991-05-24 | 1992-04-15 | Ole Buchardt | Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf |
AU694187B2 (en) * | 1994-02-07 | 1998-07-16 | Beckman Coulter, Inc. | Ligase/polymerase-mediated genetic bit analysis TM of single nucleotide polymorphisms and its use in genetic analysis |
US5985557A (en) | 1996-01-24 | 1999-11-16 | Third Wave Technologies, Inc. | Invasive cleavage of nucleic acids |
US6852487B1 (en) | 1996-02-09 | 2005-02-08 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
EP2574617B1 (de) | 1996-02-09 | 2016-04-20 | Cornell Research Foundation, Inc. | Erkennung von Nukleinsäurensequenz-Unterschieden mittels der Ligaseerkennungsreaktion mit adressierbaren Arrays |
US20020150921A1 (en) | 1996-02-09 | 2002-10-17 | Francis Barany | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
WO2000004192A1 (en) | 1998-07-17 | 2000-01-27 | Emory University | Methods for detecting and mapping genes, mutations and variant polynucleotide sequences |
EP1105398B1 (de) | 1998-08-17 | 2002-07-17 | The Dow Chemical Company | Aluminiumverbindungsmischung-enthaltende aktivatorzusammensetzung |
JP3267576B2 (ja) | 1998-11-09 | 2002-03-18 | 三光純薬株式会社 | プローブ高分子の形成方法 |
ATE418620T1 (de) | 2000-03-31 | 2009-01-15 | Sanko Junyaku Kk | Sonde zur herstellung einer polymersonde, verfahren zur herstellung einer polymersonde sowie verwendung derselben |
AU7873001A (en) | 2000-08-30 | 2002-03-13 | Sanko Junyaku Co., Ltd. | Method of detecting gene |
JPWO2003029441A1 (ja) * | 2001-09-28 | 2005-01-13 | 三光純薬株式会社 | Dnaチップのシグナル増幅方法 |
KR20040052455A (ko) | 2001-11-08 | 2004-06-23 | 산코 준야쿠 가부시키가이샤 | 유전자 증폭 반응으로 합성된 올리고뉴클레오티드에 의한자기집합체의 형성 방법, 자기집합체 및 유전자의 검출방법 |
-
2004
- 2004-02-20 US US10/546,593 patent/US7632639B2/en not_active Expired - Fee Related
- 2004-02-20 EP EP04713241A patent/EP1595953B1/de not_active Expired - Lifetime
- 2004-02-20 CA CA2516360A patent/CA2516360C/en not_active Expired - Fee Related
- 2004-02-20 AU AU2004213716A patent/AU2004213716B2/en not_active Ceased
- 2004-02-20 AT AT04713241T patent/ATE552339T1/de active
- 2004-02-20 JP JP2005502795A patent/JP4255472B2/ja not_active Expired - Fee Related
- 2004-02-20 CN CNB2004800038183A patent/CN100532550C/zh not_active Expired - Fee Related
- 2004-02-20 WO PCT/JP2004/002007 patent/WO2004074480A1/ja active Application Filing
- 2004-02-20 KR KR1020057013000A patent/KR101111568B1/ko not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
EP1595953A4 (de) | 2007-02-28 |
CA2516360A1 (en) | 2004-09-02 |
WO2004074480A1 (ja) | 2004-09-02 |
CA2516360C (en) | 2012-07-10 |
EP1595953A1 (de) | 2005-11-16 |
JP4255472B2 (ja) | 2009-04-15 |
JPWO2004074480A1 (ja) | 2006-06-01 |
KR20050106398A (ko) | 2005-11-09 |
CN1748028A (zh) | 2006-03-15 |
EP1595953B1 (de) | 2012-04-04 |
KR101111568B1 (ko) | 2012-03-05 |
AU2004213716B2 (en) | 2008-02-28 |
US20060210983A1 (en) | 2006-09-21 |
AU2004213716A1 (en) | 2004-09-02 |
CN100532550C (zh) | 2009-08-26 |
US7632639B2 (en) | 2009-12-15 |
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