ATE516363T1 - Generischer kinase-/phosphatasetest mit einzelablesung - Google Patents

Generischer kinase-/phosphatasetest mit einzelablesung

Info

Publication number
ATE516363T1
ATE516363T1 AT08801776T AT08801776T ATE516363T1 AT E516363 T1 ATE516363 T1 AT E516363T1 AT 08801776 T AT08801776 T AT 08801776T AT 08801776 T AT08801776 T AT 08801776T AT E516363 T1 ATE516363 T1 AT E516363T1
Authority
AT
Austria
Prior art keywords
kinase
fluorophore
readout
molecule
phosphatase
Prior art date
Application number
AT08801776T
Other languages
English (en)
Inventor
Thilo Enderle
Doris Roth
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE516363T1 publication Critical patent/ATE516363T1/de

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
AT08801776T 2007-09-10 2008-09-01 Generischer kinase-/phosphatasetest mit einzelablesung ATE516363T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP07115987 2007-09-10
PCT/EP2008/007122 WO2009033580A1 (en) 2007-09-10 2008-09-01 Generic kinase/phosphatase assay with single readout

Publications (1)

Publication Number Publication Date
ATE516363T1 true ATE516363T1 (de) 2011-07-15

Family

ID=39865459

Family Applications (1)

Application Number Title Priority Date Filing Date
AT08801776T ATE516363T1 (de) 2007-09-10 2008-09-01 Generischer kinase-/phosphatasetest mit einzelablesung

Country Status (8)

Country Link
US (1) US8309310B2 (de)
EP (1) EP2201129B1 (de)
JP (1) JP5108946B2 (de)
CN (1) CN101802214A (de)
AT (1) ATE516363T1 (de)
CA (1) CA2698926C (de)
ES (1) ES2368669T3 (de)
WO (1) WO2009033580A1 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9650674B2 (en) * 2012-10-19 2017-05-16 Bioventures, Llc Nucleic acid probes and methods of using the same
KR101847264B1 (ko) * 2016-03-29 2018-04-10 국립암센터 항원 반응형 항체-형광염료 결합체 및 이를 이용한 표적 세포의 형광영상 검출방법
US11932080B2 (en) 2020-08-20 2024-03-19 Denso International America, Inc. Diagnostic and recirculation control systems and methods
US11828210B2 (en) 2020-08-20 2023-11-28 Denso International America, Inc. Diagnostic systems and methods of vehicles using olfaction
US11636870B2 (en) 2020-08-20 2023-04-25 Denso International America, Inc. Smoking cessation systems and methods
US11760169B2 (en) 2020-08-20 2023-09-19 Denso International America, Inc. Particulate control systems and methods for olfaction sensors
US11813926B2 (en) 2020-08-20 2023-11-14 Denso International America, Inc. Binding agent and olfaction sensor
US11760170B2 (en) 2020-08-20 2023-09-19 Denso International America, Inc. Olfaction sensor preservation systems and methods
US11881093B2 (en) 2020-08-20 2024-01-23 Denso International America, Inc. Systems and methods for identifying smoking in vehicles

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040171034A1 (en) * 2002-05-03 2004-09-02 Brian Agnew Compositions and methods for detection and isolation of phosphorylated molecules
US7432070B2 (en) * 2003-06-16 2008-10-07 Pierce Biotechnology, Inc. Homogenous assay for enzymatic activity
EP1674580A1 (de) * 2004-12-23 2006-06-28 F. Hoffmann-La Roche Ag Verfahren zur Identifikation von Aktivatoren und/oder Inhibitoren von enzymatischen Aktivität
US20060292653A1 (en) 2005-03-18 2006-12-28 Frauke Rininsland Metal mediated fluorescence superquenching assays for detection of phosphorylation and dephosphorylation of quencher-labeled substrates
WO2007016975A1 (en) 2005-07-29 2007-02-15 F. Hoffmann-La Roche Ag Kinase and phosphatase assays based on fret
US7585643B2 (en) * 2005-09-08 2009-09-08 Marquette University Method for detecting kinase activity with thiol reactive fluorescent reagents

Also Published As

Publication number Publication date
EP2201129B1 (de) 2011-07-13
CA2698926C (en) 2012-08-14
CA2698926A1 (en) 2009-03-19
JP2010538606A (ja) 2010-12-16
US20100203554A1 (en) 2010-08-12
JP5108946B2 (ja) 2012-12-26
CN101802214A (zh) 2010-08-11
US8309310B2 (en) 2012-11-13
ES2368669T3 (es) 2011-11-21
WO2009033580A1 (en) 2009-03-19
EP2201129A1 (de) 2010-06-30

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