ATE498008T1 - Verfahren zur herstellung eines zellstamms mit fähigkeit zur tetracyclin-induzierten genexpression oder eines zellstamms mit konditionalem gen-knockout sowie verwendung der zellstämme - Google Patents

Verfahren zur herstellung eines zellstamms mit fähigkeit zur tetracyclin-induzierten genexpression oder eines zellstamms mit konditionalem gen-knockout sowie verwendung der zellstämme

Info

Publication number
ATE498008T1
ATE498008T1 AT07830399T AT07830399T ATE498008T1 AT E498008 T1 ATE498008 T1 AT E498008T1 AT 07830399 T AT07830399 T AT 07830399T AT 07830399 T AT07830399 T AT 07830399T AT E498008 T1 ATE498008 T1 AT E498008T1
Authority
AT
Austria
Prior art keywords
gene
cell strain
interest
expression vector
tetracycline
Prior art date
Application number
AT07830399T
Other languages
English (en)
Inventor
Keiichi Shibahara
Tatsuya Ono
Hitoshi Nishijima
Original Assignee
Res Org Information & Systems
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Res Org Information & Systems filed Critical Res Org Information & Systems
Application granted granted Critical
Publication of ATE498008T1 publication Critical patent/ATE498008T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/20—Vector systems having a special element relevant for transcription transcription of more than one cistron
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT07830399T 2006-10-23 2007-10-23 Verfahren zur herstellung eines zellstamms mit fähigkeit zur tetracyclin-induzierten genexpression oder eines zellstamms mit konditionalem gen-knockout sowie verwendung der zellstämme ATE498008T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2006288020 2006-10-23
PCT/JP2007/070665 WO2008050774A1 (fr) 2006-10-23 2007-10-23 Procédé de production de souche cellulaire pouvant exprimer un gène induit par la tétracycline ou de souche cellulaire présentant une inactivation conditionnelle de gènes, et utilisation des souches cellulaires

Publications (1)

Publication Number Publication Date
ATE498008T1 true ATE498008T1 (de) 2011-02-15

Family

ID=39324571

Family Applications (1)

Application Number Title Priority Date Filing Date
AT07830399T ATE498008T1 (de) 2006-10-23 2007-10-23 Verfahren zur herstellung eines zellstamms mit fähigkeit zur tetracyclin-induzierten genexpression oder eines zellstamms mit konditionalem gen-knockout sowie verwendung der zellstämme

Country Status (6)

Country Link
US (1) US20100003686A1 (de)
EP (1) EP2085475B1 (de)
JP (1) JPWO2008050774A1 (de)
AT (1) ATE498008T1 (de)
DE (1) DE602007012460D1 (de)
WO (1) WO2008050774A1 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5576382B2 (ja) * 2008-10-01 2014-08-20 テット・システムズ・ゲーエムベーハー・ウント・カンパニー・カーゲー テトラサイクリン誘導可能な転写制御配列
US8889312B2 (en) * 2009-07-29 2014-11-18 The Invention Science Fund I, Llc Instrumented fluid-surfaced electrode
EP2431386B1 (de) 2010-09-18 2015-02-25 FBN - Leibniz-Institut für Nutztierbiologie Na+/Mg2+-Tauscher
CN109456991B (zh) * 2017-09-06 2023-05-09 华东师范大学 原儿茶酸调控的开关系统及其调控方法和应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4937190A (en) 1987-10-15 1990-06-26 Wisconsin Alumni Research Foundation Translation enhancer
US5866755A (en) * 1993-06-14 1999-02-02 Basf Aktiengellschaft Animals transgenic for a tetracycline-regulated transcriptional inhibitor
DE19635568C1 (de) * 1996-09-02 1998-03-26 Gsf Forschungszentrum Umwelt Vektorsysteme zur konditionalen Genexpression
FR2762615B1 (fr) 1997-04-28 1999-07-16 Inst Nat Sante Rech Med Nouveau site interne d'entree des ribosomes et vecteur le contenant
FI972293A (fi) 1997-05-30 1998-12-01 Joseph Atabekov Useamman kuin yhden geenin yhteisekspressiomenetelmät
US5968773A (en) * 1997-11-14 1999-10-19 Heddle; John A. System and method for regulation of gene expression
EP1969144A2 (de) * 2005-12-16 2008-09-17 The Uab Research Foundation Zusammensetzungen und verfahren in verbindung mit kontrollierter genexpression unter verwendung viraler vektoren

Also Published As

Publication number Publication date
EP2085475A1 (de) 2009-08-05
DE602007012460D1 (de) 2011-03-24
US20100003686A1 (en) 2010-01-07
JPWO2008050774A1 (ja) 2010-02-25
EP2085475B1 (de) 2011-02-09
EP2085475A4 (de) 2009-11-04
WO2008050774A1 (fr) 2008-05-02

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