ATE492653T1 - Regulierte vektoren zur steuerung der dna- hypermutabilität in eukaryotischen zellen - Google Patents

Regulierte vektoren zur steuerung der dna- hypermutabilität in eukaryotischen zellen

Info

Publication number
ATE492653T1
ATE492653T1 AT09155285T AT09155285T ATE492653T1 AT E492653 T1 ATE492653 T1 AT E492653T1 AT 09155285 T AT09155285 T AT 09155285T AT 09155285 T AT09155285 T AT 09155285T AT E492653 T1 ATE492653 T1 AT E492653T1
Authority
AT
Austria
Prior art keywords
genetically stable
clones
dominant negative
somatic cell
rendering
Prior art date
Application number
AT09155285T
Other languages
English (en)
Inventor
Luigi Grasso
Nicholas C Nicholaides
Philip M Sass
Original Assignee
Morphotek Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Morphotek Inc filed Critical Morphotek Inc
Application granted granted Critical
Publication of ATE492653T1 publication Critical patent/ATE492653T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
AT09155285T 2002-02-21 2003-02-21 Regulierte vektoren zur steuerung der dna- hypermutabilität in eukaryotischen zellen ATE492653T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US35860202P 2002-02-21 2002-02-21

Publications (1)

Publication Number Publication Date
ATE492653T1 true ATE492653T1 (de) 2011-01-15

Family

ID=27788960

Family Applications (2)

Application Number Title Priority Date Filing Date
AT09155285T ATE492653T1 (de) 2002-02-21 2003-02-21 Regulierte vektoren zur steuerung der dna- hypermutabilität in eukaryotischen zellen
AT03733833T ATE428804T1 (de) 2002-02-21 2003-02-21 Regulierte vektoren zur steuerung der dna- hypermutabilität in eukaryontischen zellen

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT03733833T ATE428804T1 (de) 2002-02-21 2003-02-21 Regulierte vektoren zur steuerung der dna- hypermutabilität in eukaryontischen zellen

Country Status (7)

Country Link
US (1) US20030165468A1 (de)
EP (2) EP1476577B1 (de)
AT (2) ATE492653T1 (de)
AU (1) AU2003239120B2 (de)
CA (1) CA2476559A1 (de)
DE (2) DE60335502D1 (de)
WO (1) WO2003074655A2 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060258007A1 (en) * 2005-05-16 2006-11-16 Nicolaides Nicholas C Regulated vectors for selection of cells exhibiting desired phenotypes
WO2007121396A2 (en) * 2006-04-17 2007-10-25 Morphotek, Inc. Whole genome evolution technology applied to improve protein and antibody yields by cells
JP2009534040A (ja) * 2006-04-19 2009-09-24 ワイス Ｉｒｅｓ配列を標的とする短鎖干渉ｒｎａ二本鎖及びそのための使用

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4634665A (en) 1980-02-25 1987-01-06 The Trustees Of Columbia University In The City Of New York Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4656134A (en) 1982-01-11 1987-04-07 Board Of Trustees Of Leland Stanford Jr. University Gene amplification in eukaryotic cells
US5464764A (en) * 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
US5952221A (en) * 1996-03-06 1999-09-14 Avigen, Inc. Adeno-associated virus vectors comprising a first and second nucleic acid sequence
US6146894A (en) * 1998-04-14 2000-11-14 The Johns Hopkins University Method for generating hypermutable organisms
EP1268765B1 (de) * 2000-02-11 2008-08-13 The Johns Hopkins University Methode zur herstellung hypermutierender bakterien
WO2001061012A1 (en) * 2000-02-18 2001-08-23 Nicolaides Nicholas C A method for generating hypermutable plants
DK1259628T5 (da) * 2000-02-23 2018-06-14 Univ Johns Hopkins Fremgangsmåde til dannelse af hypermutabel gær
CA2408826C (en) * 2000-05-12 2012-08-14 The Johns Hopkins University A method for generating hypermutable organisms
CA2476480A1 (en) * 2002-02-21 2003-09-04 Morphotek Inc. Methods of making hypermutable cells using pmsr homologs
US7604994B2 (en) * 2003-09-03 2009-10-20 Morphotek, Inc. Genetically altered antibody-producing cell lines with improved antibody characteristics

Also Published As

Publication number Publication date
WO2003074655A3 (en) 2004-03-25
EP2110440B1 (de) 2010-12-22
ATE428804T1 (de) 2009-05-15
EP1476577A4 (de) 2006-05-31
CA2476559A1 (en) 2003-09-12
AU2003239120B2 (en) 2008-04-03
US20030165468A1 (en) 2003-09-04
DE60327172D1 (de) 2009-05-28
EP2110440A3 (de) 2009-10-28
DE60335502D1 (de) 2011-02-03
EP1476577A2 (de) 2004-11-17
EP1476577B1 (de) 2009-04-15
WO2003074655A2 (en) 2003-09-12
AU2003239120A1 (en) 2003-09-16
EP2110440A2 (de) 2009-10-21

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