ATE484591T1 - Minimale dna sequenz, die als chromatin-isolator wirkt, und deren verwendung für die protein- expression - Google Patents

Minimale dna sequenz, die als chromatin-isolator wirkt, und deren verwendung für die protein- expression

Info

Publication number
ATE484591T1
ATE484591T1 AT04791263T AT04791263T ATE484591T1 AT E484591 T1 ATE484591 T1 AT E484591T1 AT 04791263 T AT04791263 T AT 04791263T AT 04791263 T AT04791263 T AT 04791263T AT E484591 T1 ATE484591 T1 AT E484591T1
Authority
AT
Austria
Prior art keywords
dna sequence
protein expression
chromatin insulator
minimal dna
sequence acting
Prior art date
Application number
AT04791263T
Other languages
English (en)
Inventor
Philippe Chatellard
Markus Imhof
Original Assignee
Merck Serono Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Serono Sa filed Critical Merck Serono Sa
Application granted granted Critical
Publication of ATE484591T1 publication Critical patent/ATE484591T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/465—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from birds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AT04791263T 2003-10-21 2004-10-20 Minimale dna sequenz, die als chromatin-isolator wirkt, und deren verwendung für die protein- expression ATE484591T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP03103890 2003-10-21
PCT/EP2004/052591 WO2005040384A1 (en) 2003-10-21 2004-10-20 Minimal dna sequence acting as a chromatin insulator and its use in protein expression

Publications (1)

Publication Number Publication Date
ATE484591T1 true ATE484591T1 (de) 2010-10-15

Family

ID=34486337

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04791263T ATE484591T1 (de) 2003-10-21 2004-10-20 Minimale dna sequenz, die als chromatin-isolator wirkt, und deren verwendung für die protein- expression

Country Status (17)

Country Link
US (1) US8133699B2 (de)
EP (1) EP1675956B1 (de)
JP (1) JP4745972B2 (de)
AT (1) ATE484591T1 (de)
AU (1) AU2004284243B2 (de)
CA (1) CA2541523A1 (de)
CY (1) CY1110868T1 (de)
DE (1) DE602004029598D1 (de)
DK (1) DK1675956T3 (de)
ES (1) ES2354160T3 (de)
HR (1) HRP20100560T1 (de)
IL (1) IL175021A (de)
NO (1) NO338767B1 (de)
PL (1) PL1675956T3 (de)
PT (1) PT1675956E (de)
SI (1) SI1675956T1 (de)
WO (1) WO2005040384A1 (de)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2435972C (en) 2001-01-26 2011-09-13 University Of Lausanne Matrix attachment regions and methods for use thereof
MXPA05009608A (es) 2003-03-11 2006-03-21 Applied Research Systems Vectores de expresion que comprenden el promotor mcmv ie2.
JP5074030B2 (ja) * 2003-05-13 2012-11-14 メルク セローノ ソシエテ アノニム Ｉｌ−１８結合タンパク質の活性変異体とその医学的応用
PL2287302T3 (pl) 2003-10-24 2013-05-31 Selexis Sa Wysokowydajny transfer genu i ekspresja w komórkach ssaczych przez procedurę wielokrotnej transfekcji sekwencji regionu przylegania do regionu
US7820800B2 (en) * 2003-11-05 2010-10-26 Ares Trading S.A. Process for the purification of IL-18 binding protein
ME01218B (me) * 2004-03-01 2013-06-20 Ares Trading Sa Upotreba podloge za ćelijsku strukturu bez sadrzaja seruma za produkciju il-18bp u ćelijama sisara
ATE408616T1 (de) 2004-06-29 2008-10-15 Ares Trading Sa Verfahren zur aufreinigung von il-18-bindendem protein
AU2006254103B2 (en) * 2005-06-03 2012-09-06 Ares Trading S.A. Production of recombinant IL-18 binding protein
ATE529433T1 (de) 2005-06-10 2011-11-15 Ares Trading Sa Verfahren zur reinigung eines il-18-bindenden proteins
EP1760092A1 (de) * 2005-08-26 2007-03-07 Applied Research Systems ARS Holding N.V. System für Screening von Zellen mit hoher Expression von einem interessierenden Protein
FR2919616A1 (fr) * 2007-08-02 2009-02-06 Ecole Norm Superieure Lyon Polynucleotides insulateurs derives de l'element d4z4 et leurs utilisations en transgenese
WO2012139195A1 (en) 2011-04-13 2012-10-18 National Research Council Of Canada EXPRESSION SYSTEM WITH SAR ELEMENT FROM IFNα2
DK2794886T3 (en) * 2011-12-21 2017-02-27 Danisco Us Inc IMPROVEMENT OF FUNGAL Gene EXPRESSION USING ISOLATOR DNA SEQUENCES
CA2882028C (en) 2012-07-31 2023-11-07 Basil M. Hantash Human leukocyte antigen-g (hla-g) modified cells and methods
KR101591823B1 (ko) * 2013-12-27 2016-02-04 재단법인 목암생명공학연구소 증가된 유전자 발현능을 갖는 발현벡터
MY189674A (en) * 2015-10-28 2022-02-24 Sangamo Therapeutics Inc Liver-specific constructs, factor viii expression cassettes and methods of use thereof
WO2017075395A1 (en) * 2015-10-28 2017-05-04 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Tumor-specific adenovirus vectors and therapeutic uses
HRP20240603T1 (hr) 2016-07-01 2024-07-19 Resolve Therapeutics, Llc Optimizirane fuzije binukleaze i postupci njihove upotrebe
CN106754817B (zh) * 2016-11-03 2021-02-05 山西省生物研究院有限公司 一种重组自分泌运动因子的表达方法
EP3672986A1 (de) 2017-08-22 2020-07-01 Sanabio, LLC Lösliche interferonrezeptoren und ihre verwendungen
EP3796980A4 (de) 2018-05-22 2022-03-23 The Brigham & Women's Hospital, Inc. Neue therapie für morbus alzheimer
WO2020028830A1 (en) 2018-08-03 2020-02-06 Sangamo Therapeutics, Inc. Improved clinical parameters by expression of factor viii
EP3976637A4 (de) 2019-05-24 2023-07-12 The Brigham and Women's Hospital, Inc. Gentherapie für morbus alzheimer
MX2022006499A (es) * 2019-11-29 2022-08-15 Paros Bio Inc Terapia genica para trastornos neurodegenerativos.

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5859205A (en) * 1989-12-21 1999-01-12 Celltech Limited Humanised antibodies
US6113898A (en) * 1995-06-07 2000-09-05 Idec Pharmaceuticals Corporation Human B7.1-specific primatized antibodies and transfectomas expressing said antibodies
US6432700B1 (en) * 1997-03-03 2002-08-13 Cell Genesys, Inc. Adenovirus vectors containing heterologous transcription regulatory elements and methods of using same
US6194152B1 (en) * 1997-08-20 2001-02-27 Dendreon Corporation Prostate tumor polynucleotide compositions and methods of detection thereof
WO2000023606A1 (en) * 1998-10-22 2000-04-27 Medical College Of Georgia Institute, Inc. Long terminal repeat, enhancer, and insulator sequences for use in recombinant vectors
WO2001002553A2 (en) * 1999-06-30 2001-01-11 The Government Of The United States Of America As Represented By The Secretary, Department Of Health And Human Services Dna binding protein and sequence as insulators having specific enhancer blocking activity for regulation of gene expression
MXPA05009608A (es) 2003-03-11 2006-03-21 Applied Research Systems Vectores de expresion que comprenden el promotor mcmv ie2.
JP5074030B2 (ja) 2003-05-13 2012-11-14 メルク セローノ ソシエテ アノニム Ｉｌ−１８結合タンパク質の活性変異体とその医学的応用
US7820800B2 (en) 2003-11-05 2010-10-26 Ares Trading S.A. Process for the purification of IL-18 binding protein
ME01218B (me) 2004-03-01 2013-06-20 Ares Trading Sa Upotreba podloge za ćelijsku strukturu bez sadrzaja seruma za produkciju il-18bp u ćelijama sisara
ATE408616T1 (de) 2004-06-29 2008-10-15 Ares Trading Sa Verfahren zur aufreinigung von il-18-bindendem protein
AU2006254103B2 (en) 2005-06-03 2012-09-06 Ares Trading S.A. Production of recombinant IL-18 binding protein
ATE529433T1 (de) 2005-06-10 2011-11-15 Ares Trading Sa Verfahren zur reinigung eines il-18-bindenden proteins

Also Published As

Publication number Publication date
AU2004284243A1 (en) 2005-05-06
PL1675956T3 (pl) 2011-06-30
SI1675956T1 (sl) 2010-12-31
EP1675956B1 (de) 2010-10-13
US20070134761A1 (en) 2007-06-14
PT1675956E (pt) 2010-11-04
EP1675956A1 (de) 2006-07-05
ES2354160T3 (es) 2011-03-10
NO20062315L (no) 2006-07-18
WO2005040384A1 (en) 2005-05-06
JP4745972B2 (ja) 2011-08-10
AU2004284243B2 (en) 2010-11-04
CY1110868T1 (el) 2015-06-10
HRP20100560T1 (hr) 2010-11-30
CA2541523A1 (en) 2005-05-06
IL175021A0 (en) 2006-08-20
JP2007508833A (ja) 2007-04-12
US8133699B2 (en) 2012-03-13
DK1675956T3 (da) 2010-11-22
IL175021A (en) 2011-07-31
DE602004029598D1 (de) 2010-11-25
NO338767B1 (no) 2016-10-17

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