ATE474066T1 - Verfahren zum nachweis von pathogenen mittels molecular beacons - Google Patents

Verfahren zum nachweis von pathogenen mittels molecular beacons

Info

Publication number
ATE474066T1
ATE474066T1 AT06808556T AT06808556T ATE474066T1 AT E474066 T1 ATE474066 T1 AT E474066T1 AT 06808556 T AT06808556 T AT 06808556T AT 06808556 T AT06808556 T AT 06808556T AT E474066 T1 ATE474066 T1 AT E474066T1
Authority
AT
Austria
Prior art keywords
detecting pathogens
molecular beacons
probes
pathogens
detecting
Prior art date
Application number
AT06808556T
Other languages
English (en)
Inventor
Csaba Jeney
Tibor Takacs
Original Assignee
Genoid Kft
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0523250A external-priority patent/GB0523250D0/en
Application filed by Genoid Kft filed Critical Genoid Kft
Application granted granted Critical
Publication of ATE474066T1 publication Critical patent/ATE474066T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/161Modifications characterised by incorporating target specific and non-target specific sites
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30Oligonucleotides characterised by their secondary structure
    • C12Q2525/301Hairpin oligonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10Detection mode being characterised by the assay principle
    • C12Q2565/107Alteration in the property of hybridised versus free label oligonucleotides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AT06808556T 2005-11-15 2006-11-15 Verfahren zum nachweis von pathogenen mittels molecular beacons ATE474066T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US73700605P 2005-11-15 2005-11-15
GB0523250A GB0523250D0 (en) 2005-11-15 2005-11-15 Method
PCT/GB2006/004266 WO2007057669A2 (en) 2005-11-15 2006-11-15 Method of detecting pathogens

Publications (1)

Publication Number Publication Date
ATE474066T1 true ATE474066T1 (de) 2010-07-15

Family

ID=37684956

Family Applications (1)

Application Number Title Priority Date Filing Date
AT06808556T ATE474066T1 (de) 2005-11-15 2006-11-15 Verfahren zum nachweis von pathogenen mittels molecular beacons

Country Status (12)

Country Link
US (1) US7993881B2 (de)
EP (1) EP1844164B1 (de)
JP (1) JP2009515545A (de)
KR (1) KR20080084809A (de)
AT (1) ATE474066T1 (de)
AU (1) AU2006314297B2 (de)
CA (1) CA2629564C (de)
DE (1) DE602006015419D1 (de)
EA (1) EA014648B1 (de)
NO (1) NO20082657L (de)
PL (1) PL1844164T3 (de)
WO (1) WO2007057669A2 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2011204313A1 (en) * 2010-01-08 2012-07-26 Qiagen Gaithersburg, Inc. Materials and methods for isothermal nucleic acid amplification
EP2536853B1 (de) * 2010-02-16 2018-02-14 Becton Dickinson and Company Assay zur erkennung eng verwandter serotypen des humanen papillomavirus (hpv)
CN103814139B (zh) * 2011-04-01 2018-11-13 澳康姆生物实验室公司 用于检测无细胞的病原体特异性核酸的方法和试剂盒
US20140212869A1 (en) * 2013-01-25 2014-07-31 Agilent Technologies, Inc. Nucleic Acid Proximity Assay Involving the Formation of a Three-way junction
US20170204481A1 (en) * 2014-12-22 2017-07-20 Enzo Biochem, Inc. Comprehensive and comparative flow cytometry-based methods for identifying the state of a biological system
GB201511470D0 (en) * 2015-06-30 2015-08-12 Cellcall Kft HPV detection method
US10655190B2 (en) * 2016-03-11 2020-05-19 Roche Molecular Systems, Inc. Compositions and methods for detection of Zika virus
WO2020050350A1 (ja) * 2018-09-07 2020-03-12 株式会社ヘルスケアシステムズ ヒトパピローマウイルスの検出方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2052413C (en) 1990-09-28 2004-07-13 Jeffrey L. Joseph Nucleotides sequences useful as type-specific probes, pcr primers and lcr probes for the amplifications and detection of human papilloma virus, and related kits and methods
FR2678284B1 (fr) * 1991-06-28 1993-10-08 Pasteur Institut Sequences d'adn derivees du genome du papillomavirus hpv42, application de ces sequences au diagnostic in vitro d'infection par ce papillomavirus et a la production de compositions immunogenes.
IL117591A0 (en) 1995-03-30 1996-07-23 Merck & Co Inc Synthetic DNA encoding human papillomavirus type 11 L1 protein
US20020102571A1 (en) * 1997-07-24 2002-08-01 Jane Theaker Use of 2'-O-methyl RNA as hybridisation probe
EP1201771A3 (de) * 1997-09-16 2002-10-23 Innogenetics N.V. Erkennung und Identifizierung von menschlichen Papillomavirus mit Hilfe von PCR und Typ-spezifischer reversen Hybridisierung
US6037130A (en) * 1998-07-28 2000-03-14 The Public Health Institute Of The City Of New York, Inc. Wavelength-shifting probes and primers and their use in assays and kits
US6440706B1 (en) 1999-08-02 2002-08-27 Johns Hopkins University Digital amplification
JPWO2002100889A1 (ja) * 2001-06-08 2004-09-24 麒麟麦酒株式会社 ヒトパピローマウイルスe7抗原の新規エピトープおよびそのエピトープにより活性化されたcd4陽性t細胞
WO2003000933A1 (en) 2001-06-25 2003-01-03 Georgia Tech Research Corporation Dual resonance energy transfer nucleic acid probes
US6898531B2 (en) * 2001-09-05 2005-05-24 Perlegen Sciences, Inc. Algorithms for selection of primer pairs
FR2832154B1 (fr) 2001-11-09 2007-03-16 Centre Nat Rech Scient Oligonucleotides inhibiteurs et leur utilisation pour reprimer specifiquement un gene
ES2282599T3 (es) * 2002-01-07 2007-10-16 Norchip A/S Metodo para detectar arnm del virus del papiloma humano.
HUP0200981A3 (en) * 2002-03-14 2004-06-28 Genoid Kft Pcr reactions with hybridizing probes using primers with broad genotype-specificity for detection of human papilloma-viruses and typing amplificates by using specifically hybridizing oligonucleotides
US6713262B2 (en) * 2002-06-25 2004-03-30 Agilent Technologies, Inc. Methods and compositions for high throughput identification of protein/nucleic acid binding pairs
WO2005012548A2 (en) * 2003-08-01 2005-02-10 Dynal Biotech Inc. Self-hybridizing multiple target nucleic acid probes and methods of use
WO2005033333A2 (en) * 2003-10-07 2005-04-14 Dako Denmark A/S Methods and compositions for the diagnosis of cancer

Also Published As

Publication number Publication date
AU2006314297A1 (en) 2007-05-24
CA2629564C (en) 2014-04-22
WO2007057669A2 (en) 2007-05-24
JP2009515545A (ja) 2009-04-16
EA200801315A1 (ru) 2008-10-30
NO20082657L (no) 2008-08-14
EA014648B1 (ru) 2010-12-30
DE602006015419D1 (de) 2010-08-26
WO2007057669A3 (en) 2007-08-02
AU2006314297B2 (en) 2012-10-18
US20090215030A1 (en) 2009-08-27
CA2629564A1 (en) 2007-05-24
KR20080084809A (ko) 2008-09-19
PL1844164T3 (pl) 2010-12-31
EP1844164A2 (de) 2007-10-17
US7993881B2 (en) 2011-08-09
EP1844164B1 (de) 2010-07-14

Similar Documents

Publication Publication Date Title
ATE474066T1 (de) Verfahren zum nachweis von pathogenen mittels molecular beacons
IL185021A0 (en) Diagnosis method of hepatic steatosis using biochemical markers
ES2422583T3 (es) Detección de parvovirus B19
MX2022014633A (es) Metodos para detectar norovirus.
WO2007133682A3 (en) 100% sequence identity detection methods for variable genomes
EP2321430B8 (de) Verfahren zur bewertung des oralkarzinomrisikos bei menschen
WO2005087951A3 (en) Reagents, methods and kits for use in deactivating nucleic acids
ATE549418T1 (de) Verfahren und nukleinsäuren zur analyse proliferativer zellerkrankungen
ATE456666T1 (de) Verfahren zur auswertung von ribonukleotidsequenzen
DE602007011301D1 (de) Verfahren zum nachweis mutanter allele mit kombination von echtzeit-pcr und rems-pcr
ATE426043T1 (de) Verfahren zur identifizierung des brustkrebsrisikos
ATE338144T1 (de) Amplifizierungs-hybridisierungsverfahren zum nachweis und zur typisierung des mes menschlichen papillomavirus
DE60328055D1 (de) Verfahren zum nachweis von pathogenen mykobakterien in klinischen proben
ATE435302T1 (de) Verfahren zur vorhersage des behandlungserfolgs mit rivastigmine basierend auf einer bestimmung des apoe genotyps eines demenzkranken
ATE532875T1 (de) Reinigungsverfahren und ihre verwendungen
ATE466105T1 (de) Verfahren, zusammensetzungen und kits zur anreicherung und zum nachweis von mikroorganismen
DE502004007244D1 (de) Verfahren zum spezifischen schnellnachweis getr nkesch dlicher mikroorganismen
SG168423A1 (en) Influenza detection method and kit therefor
DE602004030036D1 (de) Multiplexanalyse von Nukleinsäuren zur Bestimmung von Genotypen des humanen Papillomavirus
WO2006022619A3 (en) Methods for identifying risk of type ii diabetes and treatments thereof
ATE494390T1 (de) Verfahren und nukleinsäuren zur analyse von störungen der proliferation von kolonzellen
WO2005056839A3 (de) Primer und sonden zum nachweis genitaler hpv-genotypen
ATE420977T1 (de) Nachweisverfahren für ljungan-virus
CY1113242T1 (el) Μεθοδος και μεσα για ανιχνευση ανθρωπινων ιων θηλωματος
WO2009154357A3 (ko) 인유두종 바이러스 검출 방법 및 검출 키트

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1844164

Country of ref document: EP