ATE438858T1 - Methode zum auffinden von zellkolonien die eine lösliche variante eines zielproteins exprimieren - Google Patents

Methode zum auffinden von zellkolonien die eine lösliche variante eines zielproteins exprimieren

Info

Publication number
ATE438858T1
ATE438858T1 AT04735027T AT04735027T ATE438858T1 AT E438858 T1 ATE438858 T1 AT E438858T1 AT 04735027 T AT04735027 T AT 04735027T AT 04735027 T AT04735027 T AT 04735027T AT E438858 T1 ATE438858 T1 AT E438858T1
Authority
AT
Austria
Prior art keywords
target protein
soluble variant
express
filter
cell colonies
Prior art date
Application number
AT04735027T
Other languages
English (en)
Inventor
Paer Nordlund
Tobias Cornvik
Sue-Li Dahlroth
Rose-Marie Knaust
Original Assignee
Paer Nordlund
Tobias Cornvik
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Paer Nordlund, Tobias Cornvik filed Critical Paer Nordlund
Application granted granted Critical
Publication of ATE438858T1 publication Critical patent/ATE438858T1/de

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Threshing Machine Elements (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT04735027T 2003-07-02 2004-05-27 Methode zum auffinden von zellkolonien die eine lösliche variante eines zielproteins exprimieren ATE438858T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0315525.6A GB0315525D0 (en) 2003-07-02 2003-07-02 A method of screening
PCT/GB2004/002271 WO2005003784A1 (en) 2003-07-02 2004-05-27 A method of screening for cell colonies that express a soluble variant of a target protein

Publications (1)

Publication Number Publication Date
ATE438858T1 true ATE438858T1 (de) 2009-08-15

Family

ID=27676553

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04735027T ATE438858T1 (de) 2003-07-02 2004-05-27 Methode zum auffinden von zellkolonien die eine lösliche variante eines zielproteins exprimieren

Country Status (10)

Country Link
US (1) US7718381B2 (de)
EP (1) EP1639373B1 (de)
AT (1) ATE438858T1 (de)
AU (1) AU2004254096B2 (de)
CA (1) CA2531026C (de)
DE (1) DE602004022414D1 (de)
DK (1) DK1639373T3 (de)
ES (1) ES2331311T3 (de)
GB (1) GB0315525D0 (de)
WO (1) WO2005003784A1 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0419628D0 (en) * 2004-09-03 2004-10-06 European Molecular Biology Lab Embl Method for determining protein solubility
US20140024555A1 (en) * 2011-03-31 2014-01-23 Los Alamos National Security, Llc Method of identifying soluble proteins and soluble protein complexes
US9523693B2 (en) 2011-04-18 2016-12-20 Biotarget Engagement Interest Group Ab Methods for determining ligand binding to a target protein using a thermal shift assay
GB201106548D0 (en) 2011-04-18 2011-06-01 Evitraproteoma Ab A method for determining ligand binding to a target protein using a thermal shift assahy

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2152214A (en) * 1983-11-08 1985-07-31 Univ Surrey Detection of enterotoxigenic E. coli
DE4237113B4 (de) * 1992-11-03 2006-10-12 "Iba Gmbh" Peptide und deren Fusionsproteine, Expressionsvektor und Verfahren zur Herstellung eines Fusionsproteins
US20020127587A1 (en) * 2001-02-13 2002-09-12 Domenica Simms Methods and compositions for isolation of biological macromolecules

Also Published As

Publication number Publication date
DK1639373T3 (da) 2009-12-07
US20070087325A1 (en) 2007-04-19
AU2004254096B2 (en) 2009-06-11
ES2331311T3 (es) 2009-12-29
DE602004022414D1 (de) 2009-09-17
GB0315525D0 (en) 2003-08-06
EP1639373B1 (de) 2009-08-05
WO2005003784A1 (en) 2005-01-13
CA2531026A1 (en) 2005-01-13
CA2531026C (en) 2010-09-21
AU2004254096A1 (en) 2005-01-13
EP1639373A1 (de) 2006-03-29
US7718381B2 (en) 2010-05-18

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