ATE435T1 - METHOD AND REAGENT FOR THE DETERMINATION OF CREATINE KINASE. - Google Patents
METHOD AND REAGENT FOR THE DETERMINATION OF CREATINE KINASE.Info
- Publication number
- ATE435T1 ATE435T1 AT80100825T AT80100825T ATE435T1 AT E435 T1 ATE435 T1 AT E435T1 AT 80100825 T AT80100825 T AT 80100825T AT 80100825 T AT80100825 T AT 80100825T AT E435 T1 ATE435 T1 AT E435T1
- Authority
- AT
- Austria
- Prior art keywords
- reaction
- adenosine
- creatine kinase
- reagent
- determination
- Prior art date
Links
- 102000004420 Creatine Kinase Human genes 0.000 title abstract 2
- 108010042126 Creatine kinase Proteins 0.000 title abstract 2
- 239000003153 chemical reaction reagent Substances 0.000 title 1
- XTWYTFMLZFPYCI-KQYNXXCUSA-N 5'-adenylphosphoric acid Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O XTWYTFMLZFPYCI-KQYNXXCUSA-N 0.000 abstract 2
- XTWYTFMLZFPYCI-UHFFFAOYSA-N Adenosine diphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(O)=O)C(O)C1O XTWYTFMLZFPYCI-UHFFFAOYSA-N 0.000 abstract 2
- 108060001084 Luciferase Proteins 0.000 abstract 2
- 239000005089 Luciferase Substances 0.000 abstract 2
- DRBBFCLWYRJSJZ-UHFFFAOYSA-N N-phosphocreatine Chemical compound OC(=O)CN(C)C(=N)NP(O)(O)=O DRBBFCLWYRJSJZ-UHFFFAOYSA-N 0.000 abstract 2
- UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 abstract 2
- 230000015572 biosynthetic process Effects 0.000 abstract 2
- QTPILKSJIOLICA-UHFFFAOYSA-N bis[hydroxy(phosphonooxy)phosphoryl] hydrogen phosphate Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(=O)OP(O)(=O)OP(O)(O)=O QTPILKSJIOLICA-UHFFFAOYSA-N 0.000 abstract 2
- PUZPDOWCWNUUKD-UHFFFAOYSA-M sodium fluoride Chemical compound [F-].[Na+] PUZPDOWCWNUUKD-UHFFFAOYSA-M 0.000 abstract 2
- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 abstract 1
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 abstract 1
- UDMBCSSLTHHNCD-UHFFFAOYSA-N Coenzym Q(11) Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(O)=O)C(O)C1O UDMBCSSLTHHNCD-UHFFFAOYSA-N 0.000 abstract 1
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 abstract 1
- CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 abstract 1
- BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 abstract 1
- DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 abstract 1
- LNQVTSROQXJCDD-UHFFFAOYSA-N adenosine monophosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)C(OP(O)(O)=O)C1O LNQVTSROQXJCDD-UHFFFAOYSA-N 0.000 abstract 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract 1
- LIYGYAHYXQDGEP-UHFFFAOYSA-N firefly oxyluciferin Natural products Oc1csc(n1)-c1nc2ccc(O)cc2s1 LIYGYAHYXQDGEP-UHFFFAOYSA-N 0.000 abstract 1
- 238000005259 measurement Methods 0.000 abstract 1
- JJVOROULKOMTKG-UHFFFAOYSA-N oxidized Photinus luciferin Chemical compound S1C2=CC(O)=CC=C2N=C1C1=NC(=O)CS1 JJVOROULKOMTKG-UHFFFAOYSA-N 0.000 abstract 1
- 229910052760 oxygen Inorganic materials 0.000 abstract 1
- 239000001301 oxygen Substances 0.000 abstract 1
- 229920006395 saturated elastomer Polymers 0.000 abstract 1
- 239000003352 sequestering agent Substances 0.000 abstract 1
- 235000013024 sodium fluoride Nutrition 0.000 abstract 1
- 239000011775 sodium fluoride Substances 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
- C12Q1/50—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving creatine phosphokinase
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Creatine kinase is determined by the reaction of creatine phosphate with adenosine diphosphate with the formation of adenosine triphosphate, reaction of the latter with luciferin and oxygen in the presence of luciferase and diadenosine pentaphosphate with the formation of oxyluciferin and adenosine monophosphate, and measurement of the light thereupon emitted, the reaction being performed with a saturated concentration of adenosine diphosphate and substrate, in the presence of 1 to 10 millimoles per liter of AMP at pH values of 5.8 to 7.5, with at least 50 units of luciferase per test. It is desirable to operate in the presence of diadenosine pentaphosphate or sodium fluoride and a sequestering agent.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19792908054 DE2908054A1 (en) | 1979-03-02 | 1979-03-02 | METHOD AND REAGENT FOR DETERMINING CREATINE KINASE |
| EP80100825A EP0015437B1 (en) | 1979-03-02 | 1980-02-19 | Method and reagent for the determination of creatine kinase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE435T1 true ATE435T1 (en) | 1981-12-15 |
Family
ID=6064230
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT80100825T ATE435T1 (en) | 1979-03-02 | 1980-02-19 | METHOD AND REAGENT FOR THE DETERMINATION OF CREATINE KINASE. |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US4286057A (en) |
| EP (1) | EP0015437B1 (en) |
| JP (2) | JPS585678B2 (en) |
| AT (1) | ATE435T1 (en) |
| DE (2) | DE2908054A1 (en) |
| FI (1) | FI69641C (en) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4357420A (en) * | 1981-04-28 | 1982-11-02 | The United States Of America As Represented By The United States Department Of Energy | Bioluminescence methods for enzymatic determinations |
| CA1175737A (en) * | 1981-07-17 | 1984-10-09 | James A. Sanderson | Determination of creatine phosphokinase in body fluids |
| US4547461A (en) * | 1983-01-18 | 1985-10-15 | Eastman Kodak Company | Composition, analytical element and method for the quantification of creatine kinase |
| US4704355A (en) * | 1985-03-27 | 1987-11-03 | New Horizons Diagnostics Corporation | Assay utilizing ATP encapsulated within liposome particles |
| CA1251395A (en) * | 1985-05-01 | 1989-03-21 | John B. Findlay | Immunochemical method and analytical compositions and element for determination of creatine kinase-mb |
| US4767699A (en) * | 1985-05-02 | 1988-08-30 | Allied Corporation | Diagnostic reagent, kit and method employing polynucleotide displacement, separation, enzymatic cleavage and adenosine phosphate detection |
| JP2564295B2 (en) * | 1987-03-23 | 1996-12-18 | エーザイ株式会社 | Method for measuring pyruvate kinase activity for testing vitamin E deficiency |
| US4981708A (en) * | 1989-10-02 | 1991-01-01 | Enzytech, Inc. | Method of preventing browning in foods utilizing protease free latex extracts particularly from figs |
| US5283180A (en) * | 1990-07-19 | 1994-02-01 | Charm Sciences, Inc. | Bioluminescence method for the determination of pesticides |
| US5374534A (en) * | 1990-07-19 | 1994-12-20 | Charm Sciences, Inc. | Method of preparing D-luciferin derivatives |
| AT401526B (en) * | 1993-02-10 | 1996-09-25 | Scheirer Winfried | REAGENT SOLUTION TO STABILIZE LUMINESCENCE IN LUCIFERASE MEASUREMENT |
| US5734546A (en) * | 1994-09-21 | 1998-03-31 | Rohm Co. Ltd. | Capacitor element for solid electrolytic capacitor and process for making the same |
| US5817467A (en) | 1995-11-16 | 1998-10-06 | Kyowa Medex Co., Ltd. | Method for quantitatively determining creatinine kinase and a reagent therefor |
| US6171809B1 (en) | 1998-01-29 | 2001-01-09 | Packard Instrument Company | Method and compositions for detecting luciferase biological samples |
| CA2328684A1 (en) * | 2000-12-15 | 2002-06-15 | Yahia Gawad | Photon-triggered luminescent assay |
| GB0030727D0 (en) | 2000-12-15 | 2001-01-31 | Lumitech Uk Ltd | Methods and kits for detecting kinase activity |
| CN1764751B (en) | 2003-02-26 | 2012-05-30 | 株式会社岛精机制作所 | Yarn carrier of weft knitting machine |
| TW200808974A (en) * | 2006-08-02 | 2008-02-16 | Ind Tech Res Inst | Luminescence-based recipe |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3423290A (en) * | 1966-08-03 | 1969-01-21 | Nasa | Lyophilized reaction mixtures |
| US3575811A (en) * | 1968-10-23 | 1971-04-20 | Hazleton Lab Inc | Method for the detection of cancer |
| DE2302721C2 (en) * | 1973-01-19 | 1975-01-23 | Boehringer Mannheim Gmbh, 6800 Mannheim | Method for the determination of creatine kinase |
| US4080265A (en) * | 1974-08-02 | 1978-03-21 | Antonik Alan S | Method for the determination of creative phosphokinase enzyme |
| US4001088A (en) * | 1974-08-02 | 1977-01-04 | Antonik Alan S | Method for the determination of creatine phosphokinase enzyme |
| DE2828658C3 (en) * | 1978-06-29 | 1981-10-22 | Lkb-Produkter Ab, Stockholm | Method for the photometric determination of subunit B of creatine kinase and reagent therefor |
-
1979
- 1979-03-02 DE DE19792908054 patent/DE2908054A1/en not_active Withdrawn
-
1980
- 1980-02-19 DE DE8080100825T patent/DE3060087D1/en not_active Expired
- 1980-02-19 AT AT80100825T patent/ATE435T1/en not_active IP Right Cessation
- 1980-02-19 EP EP80100825A patent/EP0015437B1/en not_active Expired
- 1980-02-27 FI FI800578A patent/FI69641C/en not_active IP Right Cessation
- 1980-02-28 US US06/125,381 patent/US4286057A/en not_active Expired - Lifetime
- 1980-02-29 JP JP55024218A patent/JPS585678B2/en not_active Expired
-
1981
- 1981-11-04 JP JP56175851A patent/JPS57105199A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS55120796A (en) | 1980-09-17 |
| DE2908054A1 (en) | 1980-09-11 |
| FI800578A7 (en) | 1980-09-03 |
| EP0015437A1 (en) | 1980-09-17 |
| JPS585678B2 (en) | 1983-02-01 |
| JPS615720B2 (en) | 1986-02-20 |
| DE3060087D1 (en) | 1982-01-28 |
| FI69641B (en) | 1985-11-29 |
| US4286057A (en) | 1981-08-25 |
| JPS57105199A (en) | 1982-06-30 |
| EP0015437B1 (en) | 1981-11-25 |
| FI69641C (en) | 1986-03-10 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| REN | Ceased due to non-payment of the annual fee |