ATE434T1 - DELTA 5,3-KETOSTEROID ISOMERASE AND ITS USE IN IMMUNOENZYMATIC DOSES. - Google Patents

DELTA 5,3-KETOSTEROID ISOMERASE AND ITS USE IN IMMUNOENZYMATIC DOSES.

Info

Publication number
ATE434T1
ATE434T1 AT79400542T AT79400542T ATE434T1 AT E434 T1 ATE434 T1 AT E434T1 AT 79400542 T AT79400542 T AT 79400542T AT 79400542 T AT79400542 T AT 79400542T AT E434 T1 ATE434 T1 AT E434T1
Authority
AT
Austria
Prior art keywords
antibody
delta
reading
immunoenzymatic
antigen
Prior art date
Application number
AT79400542T
Other languages
German (de)
Inventor
Jean-Claude Nicolas
Beatrice Terouanne
Bernard Descomps
Andre Crastes De Paulet
Original Assignee
Institut National De La Sante Et De La Recherche Medicale (Inserm)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut National De La Sante Et De La Recherche Medicale (Inserm) filed Critical Institut National De La Sante Et De La Recherche Medicale (Inserm)
Application granted granted Critical
Publication of ATE434T1 publication Critical patent/ATE434T1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G01N33/539—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody involving precipitating reagent, e.g. ammonium sulfate
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/743—Steroid hormones
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/964—Chemistry: molecular biology and microbiology including enzyme-ligand conjugate production, e.g. reducing rate of nonproductive linkage
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/971—Capture of complex after antigen-antibody reaction
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/808—Automated or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/815—Test for named compound or class of compounds
    • Y10S436/817—Steroids or hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Endocrinology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Steroid Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to a novel enzymatic labelling agent for immunoenzymatic assays. The labelling agent is DELTA 5, 3-keto-steroid isomerase. The enzymatic activity bound to the antibody can be revealed advantageously by: (1) direct reading on the supernatent liquor or on the cake obtained by precipitation by means of a solvent, such as polyethylene glycol, with the use of soluble antibodies; (2) reading in solution after liberation of the enzyme from the antigen-antibody complex by a reducing agent, such as dithiotreitol; (3) immediate reading after filtration of a substrate solution on the insolubilized antibody-antigen-enzyme complex. The method can be used for immunoenzymatic assays automatically on substances present in very low concentrations, such as hypophysial hormones and plasmatic steroids.
AT79400542T 1978-08-02 1979-07-31 DELTA 5,3-KETOSTEROID ISOMERASE AND ITS USE IN IMMUNOENZYMATIC DOSES. ATE434T1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR7822867A FR2432713A1 (en) 1978-08-02 1978-08-02 APPLICATION OF D5,3-CETO STEROIDE ISOMERASE IN IMMUNOENZYMATIC ASSAYS
EP79400542A EP0007879B1 (en) 1978-08-02 1979-07-31 Delta 5,3-cetosteroid isomerase and its use in immuno-enzyme quantifications

Publications (1)

Publication Number Publication Date
ATE434T1 true ATE434T1 (en) 1981-12-15

Family

ID=9211503

Family Applications (1)

Application Number Title Priority Date Filing Date
AT79400542T ATE434T1 (en) 1978-08-02 1979-07-31 DELTA 5,3-KETOSTEROID ISOMERASE AND ITS USE IN IMMUNOENZYMATIC DOSES.

Country Status (6)

Country Link
US (1) US4298686A (en)
EP (1) EP0007879B1 (en)
JP (1) JPS5547450A (en)
AT (1) ATE434T1 (en)
DE (1) DE2961445D1 (en)
FR (1) FR2432713A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4847194A (en) * 1987-03-13 1989-07-11 Becton, Dickinson And Company Colorimetric detection of delta-5-3-ketosteroid isomerase and immunoassay based thereon
JPH02146164U (en) * 1989-05-10 1990-12-12
DE9216110U1 (en) * 1992-11-26 1993-01-28 Biolab GmbH, 80995 München Progesterone rapid test for humans and pets
WO2008010577A1 (en) 2006-07-20 2008-01-24 Sumco Techxiv Corporation Method of dopant injection, n-type silicon single-crystal, doping apparatus and pull-up device
EP4411379A1 (en) * 2023-02-03 2024-08-07 State Research Institute Centre for Innovative Medicine Single- or multi-plexed nanoparticle-based microvolume biomarker immunodetection by fluorescence spectroscopy

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4067774A (en) * 1971-05-14 1978-01-10 Syva Company Compounds for enzyme amplification assay
NL171930C (en) * 1972-05-11 1983-06-01 Akzo Nv METHOD FOR DETERMINING AND DETERMINING BITES AND TEST PACKAGING.
DE2418978A1 (en) * 1974-04-19 1975-10-30 Boehringer Mannheim Gmbh STEROID DELTA ISOMERASE
SE420733B (en) * 1974-05-30 1981-10-26 Exploaterings Ab Tbf GEL PRODUCT, INCLUDING THIOSULPHATE GROUPS AND SETS FOR PREPARING THEREOF
US4002532A (en) * 1974-10-21 1977-01-11 Weltman Joel K Enzyme conjugates
GB1524372A (en) * 1975-01-09 1978-09-13 Radiochemical Centre Ltd Radioassay of oestrogens
IL51224A (en) * 1976-11-19 1979-09-30 Ames Yissum Ltd Assay method for the quanititative determination of a hapten in aqueous solution
US4069106A (en) * 1977-01-13 1978-01-17 The United States Of America As Represented By The Secretary Of Agriculture Immobilization of enzymes on keratin
SE427505B (en) * 1977-03-04 1983-04-11 Pharmacia Diagnostics Ab REAGENT USE FOR IMMUNKEMIC DETERMINATION METHODS
US4218539A (en) * 1978-03-24 1980-08-19 Weltman Joel K Enzyme conjugates and method of preparation and use

Also Published As

Publication number Publication date
EP0007879A1 (en) 1980-02-06
US4298686A (en) 1981-11-03
FR2432713B1 (en) 1981-02-06
FR2432713A1 (en) 1980-02-29
DE2961445D1 (en) 1982-01-28
JPS5547450A (en) 1980-04-03
EP0007879B1 (en) 1981-11-25

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UEP Publication of translation of european patent specification