GB1401297A - Enzyme aplification assay - Google Patents

Enzyme aplification assay

Info

Publication number
GB1401297A
GB1401297A GB2277872A GB2277872A GB1401297A GB 1401297 A GB1401297 A GB 1401297A GB 2277872 A GB2277872 A GB 2277872A GB 2277872 A GB2277872 A GB 2277872A GB 1401297 A GB1401297 A GB 1401297A
Authority
GB
Grant status
Application
Patent type
Prior art keywords
ligand
enzyme
receptor
activity
bound
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB2277872A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SYNTEX ENERGY RES
SYNTEX ENERGY RESEARCH Inc
VARIAN ENERGY
Original Assignee
SYNTEX ENERGY RES
SYNTEX ENERGY RESEARCH Inc
VARIAN ENERGY
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/948Sedatives, e.g. cannabinoids, barbiturates
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531Production of immunochemical test materials
    • G01N33/532Production of labelled immunochemicals
    • G01N33/535Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/946CNS-stimulants, e.g. cocaine, amphetamines
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/9486Analgesics, e.g. opiates, aspirine

Abstract

1401297 Analysis using enzyme bound hapten etc SYNTEX ENERGY RESEARCH Inc and VARIAN ENERGY 15 May 1972 [14 May 1971] 22778/72 Heading G1B A method of detecting a ligand in a medium comprises contacting in a liquid phase the medium, an enzyme-bound ligand and a soluble receptor for the ligand, the enzyme-bound ligand being capable of competing with the ligand for the reception sites of the receptor and comparing the enzyme activity then obtained with that obtained for a mixture of the same amounts of enzymebound ligand and of receptor. The interaction of the enzyme-bound ligand with receptor reduces the enzyme activity. The ligand may be a monomeric or polymeric material, especially a drug, a cell or virus. The receptor may be an enzyme, antibody (obtained when the ligand is hapten using as antigen the ligand linked to protein), RNA, DNA, or a naturally occurring receptor such as thyrobinding globulin. Examples disclose the detection of codeine using carboxymethyl morphine-amylase and morphine antiserum assaying the amylase using a suspension of dyed amylose, the measurement of Pysozyme activity in morphine-lysozyme using a suspension of dried bacteria, Micrococcus lysodeikticus in sodium phosphate pH 7.0, the effect of addition of meperidine on the inhibition of activity in lysozyme-meperidine by addition of BSA-meperidine antiserum, the effect of addition of phenobarbital on the inhibition of activity in a phenobarbital-lysozyme BSA-N-carboxymethyl phenobarbital antiserum, the detection of testosterone using testosterone-3-carboxymethyl oxime-maleate dehydrogenase and antibody, assaying using oxalacetic acid and NADH, the assay of barbiturate in a synthetic urine (described) using phenobarbital - or secobarbital-lysozyme and antibodies to BSA, conjugates of analogues to these barbitals, a substrate for determination of horse-radish peroxidase (HRP) prepared by forming a leuco-dye by reaction of toluic acid p,p' -dimethylamino benzophenone, converting this to the mixed anhydride and adding this with pyridine to a DMF solution of polyvinylalcohol at 100‹C, the resulting polymer giving a blue colour with HRP. The preparation of other enzyme-bound ligands is described.
GB2277872A 1971-05-14 1972-05-15 Enzyme aplification assay Expired GB1401297A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US14360971 true 1971-05-14 1971-05-14

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US05649942 US4039385A (en) 1972-05-08 1976-01-19 Cardiac glycoside enzyme conjugates

Publications (1)

Publication Number Publication Date
GB1401297A true true GB1401297A (en) 1975-07-16

Family

ID=22504809

Family Applications (2)

Application Number Title Priority Date Filing Date
GB345175A Expired GB1401298A (en) 1971-05-14 1972-05-15 Ligands bonded to enzymes
GB2277872A Expired GB1401297A (en) 1971-05-14 1972-05-15 Enzyme aplification assay

Family Applications Before (1)

Application Number Title Priority Date Filing Date
GB345175A Expired GB1401298A (en) 1971-05-14 1972-05-15 Ligands bonded to enzymes

Country Status (7)

Country Link
JP (1) JPS5327763B1 (en)
CA (1) CA1003770A (en)
DE (1) DE2223385C3 (en)
ES (1) ES402720A1 (en)
FR (1) FR2139505A5 (en)
GB (2) GB1401298A (en)
NL (1) NL170564C (en)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1981001414A1 (en) * 1979-11-19 1981-05-28 Charles Hospital Dev An improved method of non homogenous enzyme immunoassay
EP0095286A1 (en) * 1982-05-26 1983-11-30 Boots-Celltech Diagnostics Limited Immunoassay
EP0156641A2 (en) * 1984-03-26 1985-10-02 London Biotechnology Limited Enzymic method of detecting analytes and novel substrates therefor
EP0185432A2 (en) * 1984-12-21 1986-06-25 SCLAVO S.p.A. Immunoenzymatic method for the determination of analytes, and composition suitable to the purpose
US5137804A (en) * 1988-05-10 1992-08-11 E. I. Du Pont De Nemours And Company Assay device and immunoassay
US5196306A (en) * 1989-03-29 1993-03-23 E. I. Du Pont De Nemours And Company Method for the detection or quantitation of an analyte using an analyte dependent enzyme activation system
US5374524A (en) * 1988-05-10 1994-12-20 E. I. Du Pont De Nemours And Company Solution sandwich hybridization, capture and detection of amplified nucleic acids
EP2418492A1 (en) * 2010-08-10 2012-02-15 Randox Laboratories Ltd. Meprobamate immunoassay

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1028643A (en) * 1975-02-20 1978-03-28 Judith I. Blakemore Polylodothyronine immunoassay
US4104367A (en) * 1976-10-13 1978-08-01 Hoffmann-La Roche Inc. Radioimmunoassay for methadone
US4220450A (en) * 1978-04-05 1980-09-02 Syva Company Chemically induced fluorescence immunoassay
DE2923139A1 (en) * 1979-06-07 1980-12-18 Boehringer Mannheim Gmbh A process for preparing immunologically active enzyme-labeled conjugates
US4833073A (en) * 1987-01-27 1989-05-23 Hoffmann-La Roche Inc. Immunoassay for tetrahydrocannabinol metabolites

Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1981001414A1 (en) * 1979-11-19 1981-05-28 Charles Hospital Dev An improved method of non homogenous enzyme immunoassay
EP0040224A1 (en) * 1979-11-19 1981-11-25 The Prince Charles Hospital Development Centre Trust An improved method of non homogenous enzyme immunoassay
EP0095286A1 (en) * 1982-05-26 1983-11-30 Boots-Celltech Diagnostics Limited Immunoassay
GB2120785A (en) * 1982-05-26 1983-12-07 Celltech Ltd Enzyme immunoassay
US4587212A (en) * 1982-05-26 1986-05-06 Boots-Celltech Diagnostics Limited Immunoassay
EP0156641A2 (en) * 1984-03-26 1985-10-02 London Biotechnology Limited Enzymic method of detecting analytes and novel substrates therefor
US4745054A (en) * 1984-03-26 1988-05-17 London Biotechnology Limited Enzymic method of detecting analytes and novel substrates therefor
EP0156641A3 (en) * 1984-03-26 1987-12-02 London Biotechnology Limited Enzymic method of detecting analytes and novel substrates therefor
EP0185432A3 (en) * 1984-12-21 1987-01-07 SCLAVO S.p.A. Immunoenzymatic method for the determination of analytes, and composition suitable to the purpose
EP0185432A2 (en) * 1984-12-21 1986-06-25 SCLAVO S.p.A. Immunoenzymatic method for the determination of analytes, and composition suitable to the purpose
US5137804A (en) * 1988-05-10 1992-08-11 E. I. Du Pont De Nemours And Company Assay device and immunoassay
US5374524A (en) * 1988-05-10 1994-12-20 E. I. Du Pont De Nemours And Company Solution sandwich hybridization, capture and detection of amplified nucleic acids
US5391478A (en) * 1988-05-10 1995-02-21 E. I. Du Pont De Nemours And Company Assay device and immunoassay
US5196306A (en) * 1989-03-29 1993-03-23 E. I. Du Pont De Nemours And Company Method for the detection or quantitation of an analyte using an analyte dependent enzyme activation system
US5583001A (en) * 1989-03-29 1996-12-10 E. I. Du Pont De Nemours And Company Method for detection or quantitation of an analyte using an analyte dependent enzyme activation system
US5731158A (en) * 1989-03-29 1998-03-24 E. I. Du Pont De Nemours And Company Catalyzed reporter deposition
EP2418492A1 (en) * 2010-08-10 2012-02-15 Randox Laboratories Ltd. Meprobamate immunoassay

Also Published As

Publication number Publication date Type
DE2223385C3 (en) 1984-10-25 grant
FR2139505A5 (en) 1973-01-05 application
NL7206434A (en) 1972-11-16 application
NL170564B (en) 1982-06-16 application
CA1003770A1 (en) grant
GB1401298A (en) 1975-07-16 application
JPS5327763B1 (en) 1978-08-10 grant
ES402720A1 (en) 1976-12-01 application
DE2223385B2 (en) 1979-02-22 application
CA1003770A (en) 1977-01-18 grant
NL170564C (en) 1982-11-16 grant
DE2223385A1 (en) 1973-01-04 application

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Legal Events

Date Code Title Description
PS Patent sealed
PE20 Patent expired after termination of 20 years