ATE424448T1 - TEST METHODS FOR CONDITION-DEPENDENT CALCIUM CHANNEL AGONISTS/ANTAGONISTS - Google Patents

TEST METHODS FOR CONDITION-DEPENDENT CALCIUM CHANNEL AGONISTS/ANTAGONISTS

Info

Publication number
ATE424448T1
ATE424448T1 AT03774653T AT03774653T ATE424448T1 AT E424448 T1 ATE424448 T1 AT E424448T1 AT 03774653 T AT03774653 T AT 03774653T AT 03774653 T AT03774653 T AT 03774653T AT E424448 T1 ATE424448 T1 AT E424448T1
Authority
AT
Austria
Prior art keywords
methods
antagonists
condition
test methods
calcium channel
Prior art date
Application number
AT03774653T
Other languages
German (de)
Inventor
Menghang Xia
Thomas Connolly
Paul Bennett
Charles Cohen
Original Assignee
Merck & Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck & Co Inc filed Critical Merck & Co Inc
Application granted granted Critical
Publication of ATE424448T1 publication Critical patent/ATE424448T1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells

Abstract

Methods of identifying activators and inhibitors of voltage-gated ion channels are provided in which the methods employ cells transfected with a voltage-gated ion channel of interest and a corollary channel to control the membrane potential of the cells by changing extracellular ion concentration. This allows for more convenient, more precise experimental manipulation of these transitions, and, coupled with efficient methods of detecting the result of ion flux through the channels, provides methods that are especially suitable for high throughput screening.
AT03774653T 2002-10-10 2003-10-09 TEST METHODS FOR CONDITION-DEPENDENT CALCIUM CHANNEL AGONISTS/ANTAGONISTS ATE424448T1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US41801702P 2002-10-10 2002-10-10

Publications (1)

Publication Number Publication Date
ATE424448T1 true ATE424448T1 (en) 2009-03-15

Family

ID=32094136

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03774653T ATE424448T1 (en) 2002-10-10 2003-10-09 TEST METHODS FOR CONDITION-DEPENDENT CALCIUM CHANNEL AGONISTS/ANTAGONISTS

Country Status (6)

Country Link
US (1) US20060014206A1 (en)
EP (1) EP1560915B1 (en)
AT (1) ATE424448T1 (en)
CA (1) CA2501233A1 (en)
DE (1) DE60326482D1 (en)
WO (1) WO2004033647A2 (en)

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US9274099B2 (en) 2005-07-22 2016-03-01 The Board Of Trustees Of The Leland Stanford Junior University Screening test drugs to identify their effects on cell membrane voltage-gated ion channel
US20090093403A1 (en) 2007-03-01 2009-04-09 Feng Zhang Systems, methods and compositions for optical stimulation of target cells
US10052497B2 (en) 2005-07-22 2018-08-21 The Board Of Trustees Of The Leland Stanford Junior University System for optical stimulation of target cells
US8926959B2 (en) 2005-07-22 2015-01-06 The Board Of Trustees Of The Leland Stanford Junior University System for optical stimulation of target cells
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KR100716103B1 (en) 2005-10-28 2007-05-09 한국과학기술연구원 Cell-based high-throughput screening method of t-type calcium channel blockers
WO2008086470A1 (en) 2007-01-10 2008-07-17 The Board Of Trustees Of The Leland Stanford Junior University System for optical stimulation of target cells
US8401609B2 (en) 2007-02-14 2013-03-19 The Board Of Trustees Of The Leland Stanford Junior University System, method and applications involving identification of biological circuits such as neurological characteristics
US10035027B2 (en) 2007-10-31 2018-07-31 The Board Of Trustees Of The Leland Stanford Junior University Device and method for ultrasonic neuromodulation via stereotactic frame based technique
US10434327B2 (en) 2007-10-31 2019-10-08 The Board Of Trustees Of The Leland Stanford Junior University Implantable optical stimulators
KR20110005280A (en) 2008-04-23 2011-01-17 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 Systems, methods and compositions for optical stimulation of target cells
EP2294208B1 (en) 2008-05-29 2013-05-08 The Board of Trustees of The Leland Stanford Junior University Cell line, system and method for optical control of secondary messengers
JP5887136B2 (en) 2008-06-17 2016-03-16 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー Apparatus and method for controlling cell development
MY156389A (en) 2008-06-17 2016-02-15 Univ Leland Stanford Junior Methods, systems and devices for optical stimulation of target cells using an optical transmission element
WO2010006049A1 (en) 2008-07-08 2010-01-14 The Board Of Trustees Of The Leland Stanford Junior University Materials and approaches for optical stimulation of the peripheral nervous system
NZ602416A (en) 2008-11-14 2014-08-29 Univ Leland Stanford Junior Optically-based stimulation of target cells and modifications thereto
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US20110144028A1 (en) * 2009-12-11 2011-06-16 Vinod Sharma Modulating cellular electrophysiology
AU2011227131B2 (en) 2010-03-17 2014-11-13 The Board Of Trustees Of The Leland Stanford Junior University Light-sensitive ion-passing molecules
WO2012061684A1 (en) 2010-11-05 2012-05-10 The Board Of Trustees Of The Leland Stanford Junior University Upconversion of light for use in optogenetic methods
EP2635109A4 (en) 2010-11-05 2014-03-19 Univ Leland Stanford Junior Optically-controlled cns dysfunction
AU2011323228B2 (en) 2010-11-05 2016-11-10 The Board Of Trustees Of The Leland Stanford Junior University Control and characterization of memory function
ES2716819T3 (en) 2010-11-05 2019-06-17 Univ Leland Stanford Junior Chimeric opposites activated by light and methods of using them
CN103476456B (en) 2010-11-05 2017-10-03 斯坦福大学托管董事会 Award the light Genetic control of corelation behaviour
CA2816990A1 (en) 2010-11-05 2012-05-10 The Board Of Trustees Of The Leland Stanford Junior University Stabilized step function opsin proteins and methods of using the same
US8696722B2 (en) 2010-11-22 2014-04-15 The Board Of Trustees Of The Leland Stanford Junior University Optogenetic magnetic resonance imaging
US9365628B2 (en) 2011-12-16 2016-06-14 The Board Of Trustees Of The Leland Stanford Junior University Opsin polypeptides and methods of use thereof
WO2013126521A1 (en) 2012-02-21 2013-08-29 The Board Of Trustees Of The Leland Stanford Junior University Compositions and methods for treating neurogenic disorders of the pelvic floor
KR20150108853A (en) * 2013-01-10 2015-09-30 타우 쎄라퓨틱스 엘엘씨 T-type calcium channel inhibitors for treatment of cancer
EP2968997B1 (en) 2013-03-15 2019-06-26 The Board of Trustees of the Leland Stanford Junior University Optogenetic control of behavioral state
US9636380B2 (en) 2013-03-15 2017-05-02 The Board Of Trustees Of The Leland Stanford Junior University Optogenetic control of inputs to the ventral tegmental area
EP2991491B1 (en) 2013-04-29 2019-12-25 The Board of Trustees of the Leland Stanford Junior University Devices, systems and methods for optogenetic modulation of action potentials in target cells
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WO2016209654A1 (en) 2015-06-22 2016-12-29 The Board Of Trustees Of The Leland Stanford Junior University Methods and devices for imaging and/or optogenetic control of light-responsive neurons
US11294165B2 (en) 2017-03-30 2022-04-05 The Board Of Trustees Of The Leland Stanford Junior University Modular, electro-optical device for increasing the imaging field of view using time-sequential capture

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Also Published As

Publication number Publication date
CA2501233A1 (en) 2004-04-22
DE60326482D1 (en) 2009-04-16
EP1560915B1 (en) 2009-03-04
WO2004033647A3 (en) 2004-11-04
EP1560915A2 (en) 2005-08-10
EP1560915A4 (en) 2006-11-15
WO2004033647A2 (en) 2004-04-22
US20060014206A1 (en) 2006-01-19

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