ATE411339T1 - Spezifischer nachweis von proteolytischen enzymen mittels eines hybrid-proteins - Google Patents

Spezifischer nachweis von proteolytischen enzymen mittels eines hybrid-proteins

Info

Publication number
ATE411339T1
ATE411339T1 AT02735235T AT02735235T ATE411339T1 AT E411339 T1 ATE411339 T1 AT E411339T1 AT 02735235 T AT02735235 T AT 02735235T AT 02735235 T AT02735235 T AT 02735235T AT E411339 T1 ATE411339 T1 AT E411339T1
Authority
AT
Austria
Prior art keywords
dye
cutting
specific detection
proteolytic enzymes
fluorescence
Prior art date
Application number
AT02735235T
Other languages
English (en)
Inventor
Nicole Marme
Original Assignee
Wolfrum Juergen
Knemeyer Jens Peter
Sauer Markus
Nicole Marme
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wolfrum Juergen, Knemeyer Jens Peter, Sauer Markus, Nicole Marme filed Critical Wolfrum Juergen
Application granted granted Critical
Publication of ATE411339T1 publication Critical patent/ATE411339T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Enzymes And Modification Thereof (AREA)
AT02735235T 2001-04-06 2002-04-08 Spezifischer nachweis von proteolytischen enzymen mittels eines hybrid-proteins ATE411339T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE10117430A DE10117430A1 (de) 2001-04-06 2001-04-06 Hochempfindlicher und hochspezifischer Enzymnachweis mit einer Nachweisgrenze bis in den femtomolaren Bereich

Publications (1)

Publication Number Publication Date
ATE411339T1 true ATE411339T1 (de) 2008-10-15

Family

ID=7680794

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02735235T ATE411339T1 (de) 2001-04-06 2002-04-08 Spezifischer nachweis von proteolytischen enzymen mittels eines hybrid-proteins

Country Status (7)

Country Link
US (1) US7329505B2 (de)
EP (1) EP1381624B1 (de)
AT (1) ATE411339T1 (de)
AU (1) AU2002310931A1 (de)
DE (2) DE10117430A1 (de)
ES (1) ES2315366T3 (de)
WO (1) WO2002081509A2 (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0208989D0 (en) 2002-04-19 2002-05-29 Amersham Biosciences Uk Ltd Methods for measuring enzyme activity
DE102004038163A1 (de) * 2004-08-06 2006-03-16 Diacdem Chip Technologies Gmbh Fluoreszenz-basierte Assays zur schnellen, quantitativen Analyse von Biomolekülen (Proteine und Nukleinsäuren) durch Anreicherung auf Zellen oder Beads
US9127164B2 (en) 2005-10-28 2015-09-08 Almac Sciences (Scotland) Limited Fluorescent dyes and uses thereof
GB0823315D0 (en) 2008-12-22 2009-01-28 Ge Healthcare As Fluorescent Probes
WO2012110833A2 (en) 2011-02-18 2012-08-23 Almac Sciences (Scotland) Limited Fluorescence modulators
CA2826238A1 (en) 2011-03-25 2012-10-04 Almac Sciences (Scotland) Limited Enzyme assays
GB2577626B (en) 2011-06-23 2020-09-23 Univ Texas Identifying peptides at the single molecule level
US11435358B2 (en) 2011-06-23 2022-09-06 Board Of Regents, The University Of Texas System Single molecule peptide sequencing
WO2016069124A1 (en) 2014-09-15 2016-05-06 Board Of Regents, The University Of Texas System Improved single molecule peptide sequencing
GB201122099D0 (en) 2011-12-22 2012-02-01 Almac Sciences Scotland Ltd Dyes
US8568991B2 (en) 2011-12-23 2013-10-29 General Electric Company Photoactivated chemical bleaching of dyes
US9176032B2 (en) 2011-12-23 2015-11-03 General Electric Company Methods of analyzing an H and E stained biological sample
JP2016513794A (ja) 2013-03-06 2016-05-16 ゼネラル・エレクトリック・カンパニイ Ｈ＆ｅ染色された生体試料を分析する方法
CN106146642A (zh) * 2016-06-27 2016-11-23 浙江星博生物科技股份有限公司 一种用于检测精子顶体酶活性的蛋白微球偶联物及其制备方法和应用
WO2018005229A1 (en) * 2016-06-30 2018-01-04 Dupont Nutrition Biosciences Aps In feed assay of microbial proteases using peptide substrates

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4557862A (en) * 1983-10-28 1985-12-10 University Patents, Inc. Rhodamine derivatives as fluorogenic substrates for proteinases
EP0428000A1 (de) * 1989-11-03 1991-05-22 Abbott Laboratories Fluorogene Substrate zum Nachweis von proteolytischer Enzymaktivität
US5856083A (en) * 1994-05-06 1999-01-05 Pharmacopeia, Inc. Lawn assay for compounds that affect enzyme activity or bind to target molecules

Also Published As

Publication number Publication date
DE10117430A1 (de) 2002-10-10
AU2002310931A1 (en) 2002-10-21
US20050176926A1 (en) 2005-08-11
EP1381624B1 (de) 2008-10-15
DE50212899D1 (de) 2008-11-27
ES2315366T3 (es) 2009-04-01
US7329505B2 (en) 2008-02-12
WO2002081509A3 (de) 2003-04-10
EP1381624A2 (de) 2004-01-21
WO2002081509A2 (de) 2002-10-17

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