ATE411339T1 - Spezifischer nachweis von proteolytischen enzymen mittels eines hybrid-proteins - Google Patents
Spezifischer nachweis von proteolytischen enzymen mittels eines hybrid-proteinsInfo
- Publication number
- ATE411339T1 ATE411339T1 AT02735235T AT02735235T ATE411339T1 AT E411339 T1 ATE411339 T1 AT E411339T1 AT 02735235 T AT02735235 T AT 02735235T AT 02735235 T AT02735235 T AT 02735235T AT E411339 T1 ATE411339 T1 AT E411339T1
- Authority
- AT
- Austria
- Prior art keywords
- dye
- cutting
- specific detection
- proteolytic enzymes
- fluorescence
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE10117430A DE10117430A1 (de) | 2001-04-06 | 2001-04-06 | Hochempfindlicher und hochspezifischer Enzymnachweis mit einer Nachweisgrenze bis in den femtomolaren Bereich |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE411339T1 true ATE411339T1 (de) | 2008-10-15 |
Family
ID=7680794
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT02735235T ATE411339T1 (de) | 2001-04-06 | 2002-04-08 | Spezifischer nachweis von proteolytischen enzymen mittels eines hybrid-proteins |
Country Status (7)
Country | Link |
---|---|
US (1) | US7329505B2 (de) |
EP (1) | EP1381624B1 (de) |
AT (1) | ATE411339T1 (de) |
AU (1) | AU2002310931A1 (de) |
DE (2) | DE10117430A1 (de) |
ES (1) | ES2315366T3 (de) |
WO (1) | WO2002081509A2 (de) |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB0208989D0 (en) | 2002-04-19 | 2002-05-29 | Amersham Biosciences Uk Ltd | Methods for measuring enzyme activity |
DE102004038163A1 (de) * | 2004-08-06 | 2006-03-16 | Diacdem Chip Technologies Gmbh | Fluoreszenz-basierte Assays zur schnellen, quantitativen Analyse von Biomolekülen (Proteine und Nukleinsäuren) durch Anreicherung auf Zellen oder Beads |
US9127164B2 (en) | 2005-10-28 | 2015-09-08 | Almac Sciences (Scotland) Limited | Fluorescent dyes and uses thereof |
GB0823315D0 (en) | 2008-12-22 | 2009-01-28 | Ge Healthcare As | Fluorescent Probes |
WO2012110833A2 (en) | 2011-02-18 | 2012-08-23 | Almac Sciences (Scotland) Limited | Fluorescence modulators |
CA2826238A1 (en) | 2011-03-25 | 2012-10-04 | Almac Sciences (Scotland) Limited | Enzyme assays |
GB2577626B (en) | 2011-06-23 | 2020-09-23 | Univ Texas | Identifying peptides at the single molecule level |
US11435358B2 (en) | 2011-06-23 | 2022-09-06 | Board Of Regents, The University Of Texas System | Single molecule peptide sequencing |
WO2016069124A1 (en) | 2014-09-15 | 2016-05-06 | Board Of Regents, The University Of Texas System | Improved single molecule peptide sequencing |
GB201122099D0 (en) | 2011-12-22 | 2012-02-01 | Almac Sciences Scotland Ltd | Dyes |
US8568991B2 (en) | 2011-12-23 | 2013-10-29 | General Electric Company | Photoactivated chemical bleaching of dyes |
US9176032B2 (en) | 2011-12-23 | 2015-11-03 | General Electric Company | Methods of analyzing an H and E stained biological sample |
JP2016513794A (ja) | 2013-03-06 | 2016-05-16 | ゼネラル・エレクトリック・カンパニイ | H&e染色された生体試料を分析する方法 |
CN106146642A (zh) * | 2016-06-27 | 2016-11-23 | 浙江星博生物科技股份有限公司 | 一种用于检测精子顶体酶活性的蛋白微球偶联物及其制备方法和应用 |
WO2018005229A1 (en) * | 2016-06-30 | 2018-01-04 | Dupont Nutrition Biosciences Aps | In feed assay of microbial proteases using peptide substrates |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4557862A (en) * | 1983-10-28 | 1985-12-10 | University Patents, Inc. | Rhodamine derivatives as fluorogenic substrates for proteinases |
EP0428000A1 (de) * | 1989-11-03 | 1991-05-22 | Abbott Laboratories | Fluorogene Substrate zum Nachweis von proteolytischer Enzymaktivität |
US5856083A (en) * | 1994-05-06 | 1999-01-05 | Pharmacopeia, Inc. | Lawn assay for compounds that affect enzyme activity or bind to target molecules |
-
2001
- 2001-04-06 DE DE10117430A patent/DE10117430A1/de not_active Withdrawn
-
2002
- 2002-04-08 DE DE50212899T patent/DE50212899D1/de not_active Expired - Lifetime
- 2002-04-08 ES ES02735235T patent/ES2315366T3/es not_active Expired - Lifetime
- 2002-04-08 EP EP02735235A patent/EP1381624B1/de not_active Expired - Lifetime
- 2002-04-08 AU AU2002310931A patent/AU2002310931A1/en not_active Abandoned
- 2002-04-08 US US10/474,263 patent/US7329505B2/en not_active Expired - Lifetime
- 2002-04-08 WO PCT/EP2002/003882 patent/WO2002081509A2/de not_active Application Discontinuation
- 2002-04-08 AT AT02735235T patent/ATE411339T1/de not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
DE10117430A1 (de) | 2002-10-10 |
AU2002310931A1 (en) | 2002-10-21 |
US20050176926A1 (en) | 2005-08-11 |
EP1381624B1 (de) | 2008-10-15 |
DE50212899D1 (de) | 2008-11-27 |
ES2315366T3 (es) | 2009-04-01 |
US7329505B2 (en) | 2008-02-12 |
WO2002081509A3 (de) | 2003-04-10 |
EP1381624A2 (de) | 2004-01-21 |
WO2002081509A2 (de) | 2002-10-17 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
REN | Ceased due to non-payment of the annual fee |