ATE360471T1 - Vorrichtung und verfahren zur trennung von molekülen und bewegung von fluiden mittels elektrophorese - Google Patents

Vorrichtung und verfahren zur trennung von molekülen und bewegung von fluiden mittels elektrophorese

Info

Publication number
ATE360471T1
ATE360471T1 AT01271253T AT01271253T ATE360471T1 AT E360471 T1 ATE360471 T1 AT E360471T1 AT 01271253 T AT01271253 T AT 01271253T AT 01271253 T AT01271253 T AT 01271253T AT E360471 T1 ATE360471 T1 AT E360471T1
Authority
AT
Austria
Prior art keywords
electrode
membrane
electric field
electrode zone
liquid
Prior art date
Application number
AT01271253T
Other languages
English (en)
Inventor
Philip John Roeth
Steven Anthony Botto
Benjamin John Curley
Chenicheri Hariharan Nair
Original Assignee
Life Therapeutics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Therapeutics Ltd filed Critical Life Therapeutics Ltd
Application granted granted Critical
Publication of ATE360471T1 publication Critical patent/ATE360471T1/de

Links

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D61/00Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/42Electrodialysis; Electro-osmosis ; Electro-ultrafiltration; Membrane capacitive deionization
    • B01D61/427Electro-osmosis
    • B01D61/4271Electro-osmosis comprising multiple electro-osmosis steps
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D61/00Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/42Electrodialysis; Electro-osmosis ; Electro-ultrafiltration; Membrane capacitive deionization
    • B01D61/427Electro-osmosis
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D57/00Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
    • B01D57/02Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/24Extraction; Separation; Purification by electrochemical means
    • C07K1/26Electrophoresis
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44756Apparatus specially adapted therefor
    • G01N27/44769Continuous electrophoresis, i.e. the sample being continuously introduced, e.g. free flow electrophoresis [FFE]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Electrochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Water Supply & Treatment (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Environmental & Geological Engineering (AREA)
  • Peptides Or Proteins (AREA)
  • Electrostatic Separation (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT01271253T 2000-12-21 2001-12-21 Vorrichtung und verfahren zur trennung von molekülen und bewegung von fluiden mittels elektrophorese ATE360471T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
AUPR2224A AUPR222400A0 (en) 2000-12-21 2000-12-21 Apparatus and method for separation of molecules and movementof fluids

Publications (1)

Publication Number Publication Date
ATE360471T1 true ATE360471T1 (de) 2007-05-15

Family

ID=3826258

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01271253T ATE360471T1 (de) 2000-12-21 2001-12-21 Vorrichtung und verfahren zur trennung von molekülen und bewegung von fluiden mittels elektrophorese

Country Status (8)

Country Link
US (1) US20020170831A1 (de)
EP (1) EP1358000B1 (de)
CN (1) CN1482939A (de)
AT (1) ATE360471T1 (de)
AU (1) AUPR222400A0 (de)
CA (1) CA2431429A1 (de)
DE (1) DE60128144T2 (de)
WO (1) WO2002049744A1 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AUPS063002A0 (en) * 2002-02-19 2002-03-14 Life Therapeutics Limited Low volume electrophoresis apparatus
CN107109487B (zh) 2014-10-15 2022-03-04 赛琪科学股份有限公司 自动处理核酸和电泳样品制备的装置、方法和系统
AU2016357319B2 (en) 2015-11-20 2022-03-10 Sage Science, Inc. Preparative electrophoretic method for targeted purification of genomic DNA fragments
WO2018071978A1 (en) * 2016-10-20 2018-04-26 Memphasys Limited Sperm separation by electrophoresis
CN110506203A (zh) 2017-04-07 2019-11-26 塞奇科学股份有限公司 用于通过使用集成电泳dna纯化来检测遗传结构变异的系统和方法
US20210062180A1 (en) * 2018-01-05 2021-03-04 Sage Science, Inc. Semi-automated research instrument system

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3870617A (en) * 1971-03-30 1975-03-11 Rhone Poulenc Sa Apparatus for forced flow electrophoresis
US4441978A (en) * 1981-06-29 1984-04-10 Ionics Incorporated Separation of proteins using electrodialysis - isoelectric focusing combination
DE3337669C2 (de) * 1983-10-17 1989-09-21 Carl Schleicher & Schuell Gmbh & Co Kg, 3352 Einbeck Gerät zur Elektroelution elektrisch geladener Makromoleküle
US4787982A (en) * 1986-12-09 1988-11-29 Caro Ricardo F Membrane separation apparatus and method
GB2225339A (en) * 1988-11-15 1990-05-30 Aligena Ag Separating electrically charged macromolecular compounds by forced-flow membrane electrophoresis
US5427664A (en) * 1993-07-22 1995-06-27 Stoev; Stoyan V. Free solution electrophoresis-membrane filters trapping assay apparatus and method
AUPP576598A0 (en) * 1998-09-07 1998-10-01 Life Therapeutics Limited Cassette for macromolecule purification
ATE449200T1 (de) * 2000-04-18 2009-12-15 Gradipore Ltd Trennung und behandlung von proben durch elektrophorese

Also Published As

Publication number Publication date
EP1358000B1 (de) 2007-04-25
CN1482939A (zh) 2004-03-17
EP1358000A4 (de) 2004-07-21
CA2431429A1 (en) 2002-06-27
AUPR222400A0 (en) 2001-01-25
EP1358000A1 (de) 2003-11-05
US20020170831A1 (en) 2002-11-21
DE60128144D1 (de) 2007-06-06
WO2002049744A1 (en) 2002-06-27
DE60128144T2 (de) 2008-01-03

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