ATE328109T1 - Test mit hohem durchsatz zur identifizierung von modifikatoren der genexpression - Google Patents

Test mit hohem durchsatz zur identifizierung von modifikatoren der genexpression

Info

Publication number
ATE328109T1
ATE328109T1 AT02734208T AT02734208T ATE328109T1 AT E328109 T1 ATE328109 T1 AT E328109T1 AT 02734208 T AT02734208 T AT 02734208T AT 02734208 T AT02734208 T AT 02734208T AT E328109 T1 ATE328109 T1 AT E328109T1
Authority
AT
Austria
Prior art keywords
cells
gene
compounds
gene expression
pools
Prior art date
Application number
AT02734208T
Other languages
English (en)
Inventor
Steven C Pruitt
David G Hangauer
Carleton C Stewart
Lawrence Mark Mielnicki
Original Assignee
Health Research Inc
Univ New York State Res Found
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Health Research Inc, Univ New York State Res Found filed Critical Health Research Inc
Application granted granted Critical
Publication of ATE328109T1 publication Critical patent/ATE328109T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1051Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AT02734208T 2001-05-04 2002-05-06 Test mit hohem durchsatz zur identifizierung von modifikatoren der genexpression ATE328109T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US28899401P 2001-05-04 2001-05-04

Publications (1)

Publication Number Publication Date
ATE328109T1 true ATE328109T1 (de) 2006-06-15

Family

ID=23109544

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02734208T ATE328109T1 (de) 2001-05-04 2002-05-06 Test mit hohem durchsatz zur identifizierung von modifikatoren der genexpression

Country Status (8)

Country Link
US (1) US7083919B2 (de)
EP (1) EP1392847B1 (de)
JP (1) JP2004533245A (de)
AT (1) ATE328109T1 (de)
AU (1) AU2002305391A1 (de)
CA (1) CA2446207A1 (de)
DE (1) DE60211895T2 (de)
WO (1) WO2002090498A2 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004076629A2 (en) * 2003-02-27 2004-09-10 Nucleonics Inc. METHODS AND CONSTRUCTS FOR EVALUATION OF RNAi TARGETS AND EFFECTOR MOLECULES
WO2009061837A1 (en) * 2007-11-05 2009-05-14 Novostem Therapeutics Inc Neoplastic stem cell system and method
DE102010023486A1 (de) * 2010-06-11 2011-12-15 B. Braun Avitum Ag Nachweisvorrichtung und -verfahren
US10610861B2 (en) * 2012-12-17 2020-04-07 Accellix Ltd. Systems, compositions and methods for detecting a biological condition
JP6349327B2 (ja) 2012-12-17 2018-06-27 レウコドゥックス,リミテッド 化学的状態を判定するためのシステムおよび方法
JPWO2022158516A1 (de) * 2021-01-22 2022-07-28

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5922601A (en) * 1995-01-19 1999-07-13 Biotransplant, Inc. High efficiency gene trap selection of regulated genetic loci
US5866330A (en) * 1995-09-12 1999-02-02 The Johns Hopkins University School Of Medicine Method for serial analysis of gene expression
US5695937A (en) * 1995-09-12 1997-12-09 The Johns Hopkins University School Of Medicine Method for serial analysis of gene expression
US6020192A (en) * 1996-01-18 2000-02-01 University Of Florida Humanized green fluorescent protein genes and methods
EP0818534A1 (de) 1996-07-12 1998-01-14 Janssen Pharmaceutica N.V. Menschliche Interleukine-12 Genexpressions-regulierende Sequenzen
US5928888A (en) * 1996-09-26 1999-07-27 Aurora Biosciences Corporation Methods and compositions for sensitive and rapid, functional identification of genomic polynucleotides and secondary screening capabilities
US6207371B1 (en) * 1996-10-04 2001-03-27 Lexicon Genetics Incorporated Indexed library of cells containing genomic modifications and methods of making and utilizing the same
DE19650714A1 (de) * 1996-12-06 1998-06-10 Melchner Harald Von Prof Dr Genfallen-Konstrukt zur Identifizierung und Isolierung von Genen
US6214563B1 (en) * 1997-08-01 2001-04-10 Aurora Biosciences Corporation Photon reducing agents for reducing undesired light emission in assays
CA2224475A1 (en) 1998-02-25 1999-08-25 University Of British Columbia Protein interaction and transcription factor trap
US6080576A (en) * 1998-03-27 2000-06-27 Lexicon Genetics Incorporated Vectors for gene trapping and gene activation
US6436707B1 (en) * 1998-03-27 2002-08-20 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
US20030143597A1 (en) * 2000-12-28 2003-07-31 Finney Robert E. Methods for making polynucleotide libraries, polynucleotide arrays, and cell libraries for high-throughput genomics analysis

Also Published As

Publication number Publication date
CA2446207A1 (en) 2002-11-14
DE60211895D1 (de) 2006-07-06
US20020164636A1 (en) 2002-11-07
WO2002090498A2 (en) 2002-11-14
US7083919B2 (en) 2006-08-01
EP1392847A2 (de) 2004-03-03
JP2004533245A (ja) 2004-11-04
DE60211895T2 (de) 2007-05-24
EP1392847B1 (de) 2006-05-31
WO2002090498A3 (en) 2003-05-01
AU2002305391A1 (en) 2002-11-18
EP1392847A4 (de) 2004-06-09

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