ATE226252T1 - Subtilinvarianten mit erhöhter stabilität und aktivität - Google Patents

Subtilinvarianten mit erhöhter stabilität und aktivität

Info

Publication number
ATE226252T1
ATE226252T1 AT94902206T AT94902206T ATE226252T1 AT E226252 T1 ATE226252 T1 AT E226252T1 AT 94902206 T AT94902206 T AT 94902206T AT 94902206 T AT94902206 T AT 94902206T AT E226252 T1 ATE226252 T1 AT E226252T1
Authority
AT
Austria
Prior art keywords
mutant
polypeptides
induce
gene
exhibits
Prior art date
Application number
AT94902206T
Other languages
English (en)
Inventor
J Norman Hansen
Original Assignee
Univ Maryland
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Maryland filed Critical Univ Maryland
Application granted granted Critical
Publication of ATE226252T1 publication Critical patent/ATE226252T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AT94902206T 1992-11-25 1993-11-08 Subtilinvarianten mit erhöhter stabilität und aktivität ATE226252T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/981,525 US5516682A (en) 1988-07-05 1992-11-25 Subtilin variant of enhanced stability and activity
PCT/US1993/010558 WO1994012528A1 (en) 1992-11-25 1993-11-08 Subtilin variant of enhanced stability and activity

Publications (1)

Publication Number Publication Date
ATE226252T1 true ATE226252T1 (de) 2002-11-15

Family

ID=25528432

Family Applications (1)

Application Number Title Priority Date Filing Date
AT94902206T ATE226252T1 (de) 1992-11-25 1993-11-08 Subtilinvarianten mit erhöhter stabilität und aktivität

Country Status (8)

Country Link
US (4) US5516682A (de)
EP (1) EP0670847B1 (de)
JP (1) JP3447057B2 (de)
AT (1) ATE226252T1 (de)
AU (1) AU687369B2 (de)
CA (1) CA2150025A1 (de)
DE (1) DE69332411T2 (de)
WO (1) WO1994012528A1 (de)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5516682A (en) * 1988-07-05 1996-05-14 University Of Maryland Subtilin variant of enhanced stability and activity
US5861275A (en) * 1995-09-28 1999-01-19 The University Of Maryland Lantibiotic mutants and chimeras of enhanced stability and activity, leader sequences therefor, genes encoding the same, and methods of producing and using the same
US5919695A (en) * 1997-04-22 1999-07-06 Quest International, B.V. Method and culture for inhibiting undesired microorganisms with culture of Bacillus subtilis
US6953683B2 (en) * 1997-07-18 2005-10-11 University Of Maryland Compositions and methods of inhibiting bacterial spore germination
WO1999003352A1 (en) * 1997-07-18 1999-01-28 University Of Maryland Sublancin lantibiotic produced by bacillus subtilis 168
WO2000039152A1 (en) * 1998-12-24 2000-07-06 University Of Maryland Stable subtilin and methods of manufacture
US6759205B2 (en) 2000-06-29 2004-07-06 University Of Maryland Construction of a strain of Bacillus subtilis 168 that displays the sublancin lantibiotic on the surface of the cell
US6846804B2 (en) * 2000-06-29 2005-01-25 University Of Maryland, College Park Office Of Technology Commercialization Construction of a structural variant of sublancin to facilitate its isolation and use in bioremediation of environmental contamination by gram-positive spore formers such as bacillus anthrasis
US7033766B2 (en) * 2000-06-29 2006-04-25 University Of Maryland Office Of Technology Commercialization Construction and screening of lantibody display libraries
US20050026270A1 (en) * 2001-06-20 2005-02-03 University Of Maryland Stable subtilin and methods of manufacture
US6861236B2 (en) † 2002-05-24 2005-03-01 Applied Nanosystems B.V. Export and modification of (poly)peptides in the lantibiotic way
US7153666B2 (en) * 2003-07-17 2006-12-26 General Atomics Methods and compositions for determination of glycated proteins
US7022494B2 (en) * 2003-09-19 2006-04-04 General Atomics Detection of potassium ions using ion-sensitive enzymes
US8187831B2 (en) * 2003-09-19 2012-05-29 General Atomics Determination of ions using ion-sensitive enzymes
JP2009544315A (ja) * 2006-07-25 2009-12-17 ジェネラル アトミクス 糖化ヘモグロビンのパーセントを定量するための方法
US7943385B2 (en) * 2006-07-25 2011-05-17 General Atomics Methods for assaying percentage of glycated hemoglobin
EP2283149A1 (de) * 2008-05-13 2011-02-16 General Atomics Elektrochemischer biosensor zur direkten bestimmung des prozentsatzes von glykosyliertem hämoglobin
BR112014020659A2 (pt) 2012-02-27 2017-06-27 Oragenics Inc variantes do lantibiótico mu1140 e outros lantibióti-cos com propriedades farmacológicas e características estructurais melhoradas
CN113039196A (zh) * 2018-10-31 2021-06-25 株式会社村田制作所 抗革兰氏阴性菌化合物
WO2020185562A1 (en) * 2019-03-08 2020-09-17 Fraunhofer Usa, Inc. Novel antimicrobial lantibiotic peptide and uses thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5218101A (en) * 1988-07-05 1993-06-08 The University Of Maryland Leader sequence inducing a post-translational modification of polypeptides in bacteria, and gene therefor
US5516682A (en) * 1988-07-05 1996-05-14 University Of Maryland Subtilin variant of enhanced stability and activity
IE892893A1 (en) * 1990-03-11 1991-09-25 Charles O Kane Phage-like bacteriocins
WO1992018633A1 (en) * 1991-04-11 1992-10-29 Stichting Nederlands Instituut Voor Zuivelonderzoek (Nizo) Lantibiotics similar to nisin a, lactic acid bacteria which produce such lantibiotics, method for constructing such lactic acid bacteria and method for preserving foodstuffs with the aid of these lantibiotics and these lactic acid bacteria producing lantibiotics

Also Published As

Publication number Publication date
EP0670847A4 (de) 1997-06-11
US5885811A (en) 1999-03-23
AU687369B2 (en) 1998-02-26
US5516682A (en) 1996-05-14
DE69332411D1 (de) 2002-11-21
US5576420A (en) 1996-11-19
EP0670847A1 (de) 1995-09-13
WO1994012528A1 (en) 1994-06-09
AU5665894A (en) 1994-06-22
US5914250A (en) 1999-06-22
DE69332411T2 (de) 2003-06-26
JPH08506010A (ja) 1996-07-02
EP0670847B1 (de) 2002-10-16
CA2150025A1 (en) 1994-06-09
JP3447057B2 (ja) 2003-09-16

Similar Documents

Publication Publication Date Title
ATE226252T1 (de) Subtilinvarianten mit erhöhter stabilität und aktivität
DE3378830D1 (en) Synthetic heat-stable enterotoxin polypeptide and multimers thereof
HK1150618A1 (en) Homogeneous preparations of il-29 il-29
IL77081A0 (en) Cloned human erythropoietin genes and products thereof
ES8605040A1 (es) Procedimiento para preparar un polipeptido con actividad de hirudina
IL112403A0 (en) Method for producing a heterologous polypeptide
EP0423224A4 (en) Leader sequence inducing a post-translational modification of polypeptides in bacteria, and gene therefor
ATE176500T1 (de) Sekretionsvektor, diesen enthaltene transformierte mikroorganismen und herstellung von produkten durch obigen mikroorganismus
ATE140008T1 (de) Polypeptid
AU1847092A (en) Implant compositions containing a biologically active protein, peptide or polypeptide
AU637589B2 (en) Ancrod proteins, their production and use
EP0750040A3 (de) Varianten des Makrophagen-stimulierendes-Proteins und Verfahren zur ihrer Herstellung
KR870005090A (ko) 변이주 인식사슬
EP0352707A3 (de) Expressionssystem für die Sekretion von bioaktivem, menschlichem granulozyt-makrophagem Koloniestimulierungsfaktor (GM-CSF) und anderen heterologen Proteinen aus Streptomyces
KR910700345A (ko) 사람 갑상선 기원의 재조합 C-말단 α-아미드화 효소
EP1295943A4 (de) Polypeptide mit aktivität der stimulierung von neutrophilen
ATE141093T1 (de) Polypeptid

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties