ATE189263T1 - Auswahl von effizient ziel-rns spaltenden ribozymen - Google Patents

Auswahl von effizient ziel-rns spaltenden ribozymen

Info

Publication number
ATE189263T1
ATE189263T1 AT91916167T AT91916167T ATE189263T1 AT E189263 T1 ATE189263 T1 AT E189263T1 AT 91916167 T AT91916167 T AT 91916167T AT 91916167 T AT91916167 T AT 91916167T AT E189263 T1 ATE189263 T1 AT E189263T1
Authority
AT
Austria
Prior art keywords
rna
ribozymes
cliping
selection
efficient target
Prior art date
Application number
AT91916167T
Other languages
English (en)
Inventor
Fred Russell Kramer
David Dubnau
Karl A Drlica
Abraham Pinter
Original Assignee
New York Health Res Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by New York Health Res Inst filed Critical New York Health Res Inst
Application granted granted Critical
Publication of ATE189263T1 publication Critical patent/ATE189263T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1041—Ribosome/Polysome display, e.g. SPERT, ARM
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/121—Hammerhead

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Operating, Guiding And Securing Of Roll- Type Closing Members (AREA)
AT91916167T 1990-07-16 1991-07-16 Auswahl von effizient ziel-rns spaltenden ribozymen ATE189263T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US55372990A 1990-07-16 1990-07-16
PCT/US1991/005017 WO1992001806A1 (en) 1990-07-16 1991-07-16 Selection of ribozymes that efficiently cleave target rna

Publications (1)

Publication Number Publication Date
ATE189263T1 true ATE189263T1 (de) 2000-02-15

Family

ID=24210504

Family Applications (1)

Application Number Title Priority Date Filing Date
AT91916167T ATE189263T1 (de) 1990-07-16 1991-07-16 Auswahl von effizient ziel-rns spaltenden ribozymen

Country Status (7)

Country Link
US (1) US5616459A (de)
EP (1) EP0600877B1 (de)
AT (1) ATE189263T1 (de)
AU (1) AU651380B2 (de)
CA (1) CA2087519C (de)
DE (1) DE69131942T2 (de)
WO (1) WO1992001806A1 (de)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5496698A (en) * 1992-08-26 1996-03-05 Ribozyme Pharmaceuticals, Inc. Method of isolating ribozyme targets
US6017756A (en) * 1992-05-14 2000-01-25 Ribozyme Pharmaceuticals, Inc. Method and reagent for inhibiting hepatitis B virus replication
US5693535A (en) * 1992-05-14 1997-12-02 Ribozyme Pharmaceuticals, Inc. HIV targeted ribozymes
EP0674707A4 (de) * 1992-12-04 1998-01-14 Apollon Inc Verbindungen und methoden zur behandlung von leukämien.
FR2723371B1 (fr) * 1994-08-02 1996-10-31 Inst Nat Sante Rech Med Oligonucleotide reconnaissant une sequence d'acide nucleique a structure non lineaire, application a des fins therapeutiques et procede de preparation
CA2194488A1 (en) * 1994-08-05 1996-02-15 Tod Paul Holler Method of using transdominant negative retroviral integrase in the treatment of retroviral infection
US6274369B1 (en) * 1996-02-02 2001-08-14 Invitrogen Corporation Method capable of increasing competency of bacterial cell transformation
US6139817A (en) * 1996-10-15 2000-10-31 Smithkline Beecham Corporation Method for determining gene essentiality in a pathogen
JPH10191990A (ja) * 1996-10-15 1998-07-28 Smithkline Beecham Corp 病原体に必須の遺伝子の測定法
AU5927598A (en) 1997-01-23 1998-08-18 Immusol Incorporated Gene functional analysis and discovery using randomized or target-specific ribozyme gene vector libraries
WO1998050530A2 (en) * 1997-05-09 1998-11-12 Ribozyme Pharmaceuticals, Inc. Enzymatic nucleic acids: synthesis, selection and use
US6548657B1 (en) 1997-06-09 2003-04-15 Ribozyme Pharmaceuticals, Inc. Method for screening nucleic acid catalysts
US6280936B1 (en) 1997-06-09 2001-08-28 Ribozyme Pharmaceuticals, Inc. Method for screening nucleic acid catalysts
US6183959B1 (en) 1997-07-03 2001-02-06 Ribozyme Pharmaceuticals, Inc. Method for target site selection and discovery
WO1999002192A1 (en) 1997-07-11 1999-01-21 Brandeis University Method of inducing apoptosis by reducing the level of thiamin
WO1999009045A1 (en) * 1997-08-20 1999-02-25 Somagenics, Inc. Antisense and antigene therapeutics with improved binding properties and methods for their use
US6316612B1 (en) 1997-08-22 2001-11-13 Ribozyme Pharmaceuticals, Inc. Xylofuranosly-containing nucleoside phosphoramidites and polynucleotides
JP2001519141A (ja) * 1997-10-07 2001-10-23 スミスクライン・ビーチャム・コーポレイション 遺伝子発現の調節方法
US6617438B1 (en) 1997-11-05 2003-09-09 Sirna Therapeutics, Inc. Oligoribonucleotides with enzymatic activity
US6127535A (en) 1997-11-05 2000-10-03 Ribozyme Pharmaceuticals, Inc. Nucleoside triphosphates and their incorporation into oligonucleotides
US6440695B1 (en) * 1998-04-17 2002-08-27 Whitehead Institute For Biomedical Research Method for producing diverse libraries of encoded polypeptides
DE19956568A1 (de) * 1999-01-30 2000-08-17 Roland Kreutzer Verfahren und Medikament zur Hemmung der Expression eines vorgegebenen Gens
GB9912965D0 (en) * 1999-06-03 1999-08-04 Oxford Biomedica Ltd In vivo selection method
US7829693B2 (en) * 1999-11-24 2010-11-09 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a target gene
US6541168B2 (en) * 2000-04-28 2003-04-01 Corning Incorporated Vacuum ultraviolet transmitting direct deposit vitrified silicon oxyfluoride lithography glass photomask blanks
US6808876B1 (en) * 2000-05-02 2004-10-26 Immusol, Inc. Cellular regulators of infectious agents and methods of use
US8546143B2 (en) 2001-01-09 2013-10-01 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a target gene
US20040002083A1 (en) * 2002-01-29 2004-01-01 Ye Ding Statistical algorithms for folding and target accessibility prediction and design of nucleic acids
EP1578767A4 (de) * 2002-12-04 2008-01-09 Algos Therapeutics Inc Verfahren und materialien zur modulation von trpm2
US20040209353A1 (en) * 2002-12-12 2004-10-21 Chiron Corporation Biological sample storage device and method for biological sample contamination testing
US20050026290A1 (en) * 2003-08-01 2005-02-03 Ciardi Joseph Anthony Inhibiting gene expression with dsRNA
US20050176125A1 (en) * 2003-12-23 2005-08-11 Rosetta Inpharmatics Llc Alternatively spliced isoform of CDC25A phosphatase and methods of use
US20050266469A1 (en) * 2004-05-25 2005-12-01 Raymond Christopher K Alternatively spliced isoforms of checkpoint kinase 1 (CHK1)
US7150969B2 (en) * 2004-06-04 2006-12-19 Rosetta Inpharmatics Llc Alternatively spliced isoform of acetyl-CoA carboxylase 2 (ACC2)
WO2006081529A2 (en) * 2005-01-28 2006-08-03 Nature Technology Corp. Compositions and processes for improved plasmid dna production
US10513711B2 (en) 2014-08-13 2019-12-24 Dupont Us Holding, Llc Genetic targeting in non-conventional yeast using an RNA-guided endonuclease
WO2016073433A1 (en) 2014-11-06 2016-05-12 E. I. Du Pont De Nemours And Company Peptide-mediated delivery of rna-guided endonuclease into cells
DK4144844T3 (da) 2015-10-12 2025-11-24 Dupont Us Holding Llc Beskyttede dna-templates til genmodificering og øget homolog rekombination i celler og fremgangsmåder til anvendelse deraf
CN120659627A (zh) 2022-07-29 2025-09-16 瑞泽恩制药公司 用于转铁蛋白受体（tfr）介导的脑和肌肉递送的组合物和方法
US20240182561A1 (en) 2022-11-04 2024-06-06 Regeneron Pharmaceuticals, Inc. Calcium voltage-gated channel auxiliary subunit gamma 1 (cacng1) binding proteins and cacng1-mediated delivery to skeletal muscle
JP2025538220A (ja) 2022-11-14 2025-11-26 リジェネロン・ファーマシューティカルズ・インコーポレイテッド アストロサイトへの線維芽細胞増殖因子受容体３媒介送達のための組成物および方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4680265A (en) * 1984-09-25 1987-07-14 Eli Lilly And Company Method of selecting recombinant DNA-containing streptomyces
US5037746A (en) * 1986-12-03 1991-08-06 University Patents, Inc. RNA ribozyme polymerases, and methods
US5144019A (en) * 1989-06-21 1992-09-01 City Of Hope Ribozyme cleavage of HIV-I RNA

Also Published As

Publication number Publication date
CA2087519C (en) 2002-10-22
CA2087519A1 (en) 1992-01-17
US5616459A (en) 1997-04-01
WO1992001806A1 (en) 1992-02-06
EP0600877A4 (en) 1994-08-17
AU8517591A (en) 1992-02-18
EP0600877B1 (de) 2000-01-26
DE69131942D1 (de) 2000-03-02
DE69131942T2 (de) 2000-06-29
AU651380B2 (en) 1994-07-21
EP0600877A1 (de) 1994-06-15

Similar Documents

Publication Publication Date Title
ATE189263T1 (de) Auswahl von effizient ziel-rns spaltenden ribozymen
ATE187491T1 (de) Isolierung, selektion und vermehrung von tierischen transgen-stammzellen
ES2140408T3 (es) Copolimeros de polioxipropileno/polioxietileno con actividad biologica mejorada.
DE69330945D1 (de) Tierzellenkultur
ES2082003T3 (es) Deshidrodidemnina b.
ID26620A (id) Penghambatan kinase raf yang menggunakan urea-urea heterosiklik yang disubstitusi
DE59603678D1 (de) Kohlenhydratmodifizierte cytostatika
ATE265997T1 (de) Substituierte tetrahydronaphthalin- und dihydronaphthalin-derivate mit einer retinoiden und/oder retinoid antagonist-ähnlichen biologischen aktivität
DK0540544T3 (da) Benzoesyresubstituerede derivater mad cardiovaskulær virkning
DE69702705D1 (de) Kondensierte pyrrolo(2,3-c) carbazole-6-one die die aktivität von gamma interferon potenzieren
ATE299372T1 (de) Antiparasitäre mittel
EP0763057A4 (de) Methode zur proliferation und differenzierung von b-zellen und verwendungen dafür
BG43856A3 (bg) Метод за борба с протозои
DE3486034D1 (de) Substituierte cinnamyl-2,3-dihydrobenzopyrane und cinnamylphenole als entzuendungshemmende wirkstoffe.
ATE59553T1 (de) Polyhydroxybenzoesaeure-abkoemmlinge.
BG36928A3 (bg) Метод за получаване на фармакологично активни пептиди
NL191634B (nl) Grondbewerkingsstelsel, en beiteldraagblok daarvoor.
IT8420757A0 (it) Metodi e composizioni di fumigazione.
FI944563A0 (fi) Mikro-organismeja, valmistusmenetelmä ja käyttö
DE69016693D1 (de) Züchtung von transformierten Mikroorganismen.
BG46743A3 (en) Method for preparing of alkanoyl anilides
BG35041A3 (bg) Метод за получаване на заместени 2-пиперидин-4-амино-6,7- диметоксихиназолини
MD950100A (en) Substituted oximethers method of their preparation and application for pests and fungus control
RU1771404C (ru) Панельна теплица
ES2113765T3 (es) Dimaleato de n,n-dietil-8,8-dipropil-2-azaespiro(4.5)decano-2-propanamina.

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties