ATE189263T1 - Auswahl von effizient ziel-rns spaltenden ribozymen - Google Patents

Auswahl von effizient ziel-rns spaltenden ribozymen

Info

Publication number
ATE189263T1
ATE189263T1 AT91916167T AT91916167T ATE189263T1 AT E189263 T1 ATE189263 T1 AT E189263T1 AT 91916167 T AT91916167 T AT 91916167T AT 91916167 T AT91916167 T AT 91916167T AT E189263 T1 ATE189263 T1 AT E189263T1
Authority
AT
Austria
Prior art keywords
rna
ribozymes
cliping
selection
efficient target
Prior art date
Application number
AT91916167T
Other languages
English (en)
Inventor
Fred Russell Kramer
David Dubnau
Karl A Drlica
Abraham Pinter
Original Assignee
New York Health Res Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by New York Health Res Inst filed Critical New York Health Res Inst
Application granted granted Critical
Publication of ATE189263T1 publication Critical patent/ATE189263T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1041—Ribosome/Polysome display, e.g. SPERT, ARM
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/121—Hammerhead

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Operating, Guiding And Securing Of Roll- Type Closing Members (AREA)
AT91916167T 1990-07-16 1991-07-16 Auswahl von effizient ziel-rns spaltenden ribozymen ATE189263T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US55372990A 1990-07-16 1990-07-16
PCT/US1991/005017 WO1992001806A1 (en) 1990-07-16 1991-07-16 Selection of ribozymes that efficiently cleave target rna

Publications (1)

Publication Number Publication Date
ATE189263T1 true ATE189263T1 (de) 2000-02-15

Family

ID=24210504

Family Applications (1)

Application Number Title Priority Date Filing Date
AT91916167T ATE189263T1 (de) 1990-07-16 1991-07-16 Auswahl von effizient ziel-rns spaltenden ribozymen

Country Status (7)

Country Link
US (1) US5616459A (de)
EP (1) EP0600877B1 (de)
AT (1) ATE189263T1 (de)
AU (1) AU651380B2 (de)
CA (1) CA2087519C (de)
DE (1) DE69131942T2 (de)
WO (1) WO1992001806A1 (de)

Families Citing this family (42)

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US5496698A (en) * 1992-08-26 1996-03-05 Ribozyme Pharmaceuticals, Inc. Method of isolating ribozyme targets
US5693535A (en) * 1992-05-14 1997-12-02 Ribozyme Pharmaceuticals, Inc. HIV targeted ribozymes
US6017756A (en) * 1992-05-14 2000-01-25 Ribozyme Pharmaceuticals, Inc. Method and reagent for inhibiting hepatitis B virus replication
WO1994013793A1 (en) * 1992-12-04 1994-06-23 Apollon, Inc. Compounds and methods for the treatment of leukemias
FR2723371B1 (fr) * 1994-08-02 1996-10-31 Inst Nat Sante Rech Med Oligonucleotide reconnaissant une sequence d'acide nucleique a structure non lineaire, application a des fins therapeutiques et procede de preparation
WO1996004386A1 (en) * 1994-08-05 1996-02-15 Warner-Lambert Company Method of using transdominant negative retroviral integrase in the treatment of retroviral infection
US6274369B1 (en) * 1996-02-02 2001-08-14 Invitrogen Corporation Method capable of increasing competency of bacterial cell transformation
US6139817A (en) * 1996-10-15 2000-10-31 Smithkline Beecham Corporation Method for determining gene essentiality in a pathogen
EP0837142A1 (de) * 1996-10-15 1998-04-22 Smithkline Beecham Corporation Verfahren zum Nachweis essentieller Gene eines Erregers
US6605429B1 (en) 1997-01-23 2003-08-12 Immusol, Incorporated Gene functional analysis and discovery using randomized or target-specific ribozyme gene vector libraries
AU749561B2 (en) * 1997-05-09 2002-06-27 Ribozyme Pharmaceuticals, Inc. Nucleic acid molecules having endonuclease and/or catalytic activity
US6548657B1 (en) 1997-06-09 2003-04-15 Ribozyme Pharmaceuticals, Inc. Method for screening nucleic acid catalysts
US6280936B1 (en) 1997-06-09 2001-08-28 Ribozyme Pharmaceuticals, Inc. Method for screening nucleic acid catalysts
US6183959B1 (en) 1997-07-03 2001-02-06 Ribozyme Pharmaceuticals, Inc. Method for target site selection and discovery
AU8569698A (en) 1997-07-11 1999-02-08 Brandeis University Method of inducing apoptosis by reducing the level of thiamin
EP1019429A4 (de) * 1997-08-20 2001-08-08 Somagenics Inc Antisense- und antigen-therapeutika mit verbesserten bindungseigenschaften und methoden zu ihrer anwendung
US6316612B1 (en) 1997-08-22 2001-11-13 Ribozyme Pharmaceuticals, Inc. Xylofuranosly-containing nucleoside phosphoramidites and polynucleotides
WO1999018116A1 (en) * 1997-10-07 1999-04-15 Smithkline Beecham Corporation Method for modulating gene expression
US6127535A (en) 1997-11-05 2000-10-03 Ribozyme Pharmaceuticals, Inc. Nucleoside triphosphates and their incorporation into oligonucleotides
US6617438B1 (en) 1997-11-05 2003-09-09 Sirna Therapeutics, Inc. Oligoribonucleotides with enzymatic activity
US6440695B1 (en) * 1998-04-17 2002-08-27 Whitehead Institute For Biomedical Research Method for producing diverse libraries of encoded polypeptides
DE19956568A1 (de) 1999-01-30 2000-08-17 Roland Kreutzer Verfahren und Medikament zur Hemmung der Expression eines vorgegebenen Gens
GB9912965D0 (en) * 1999-06-03 1999-08-04 Oxford Biomedica Ltd In vivo selection method
US7829693B2 (en) * 1999-11-24 2010-11-09 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a target gene
US6541168B2 (en) * 2000-04-28 2003-04-01 Corning Incorporated Vacuum ultraviolet transmitting direct deposit vitrified silicon oxyfluoride lithography glass photomask blanks
US6808876B1 (en) 2000-05-02 2004-10-26 Immusol, Inc. Cellular regulators of infectious agents and methods of use
US8546143B2 (en) 2001-01-09 2013-10-01 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a target gene
US20040002083A1 (en) * 2002-01-29 2004-01-01 Ye Ding Statistical algorithms for folding and target accessibility prediction and design of nucleic acids
AU2003298921A1 (en) * 2002-12-04 2004-06-23 Algos Therapeutics, Inc. Methods and materials for modulating trpm2
US7785782B2 (en) * 2002-12-12 2010-08-31 Novartis Vaccines And Diagnostics, Inc. Device and method for in-line blood testing using biochips
US20050026290A1 (en) * 2003-08-01 2005-02-03 Ciardi Joseph Anthony Inhibiting gene expression with dsRNA
US20050176125A1 (en) * 2003-12-23 2005-08-11 Rosetta Inpharmatics Llc Alternatively spliced isoform of CDC25A phosphatase and methods of use
US20050266469A1 (en) * 2004-05-25 2005-12-01 Raymond Christopher K Alternatively spliced isoforms of checkpoint kinase 1 (CHK1)
US7150969B2 (en) * 2004-06-04 2006-12-19 Rosetta Inpharmatics Llc Alternatively spliced isoform of acetyl-CoA carboxylase 2 (ACC2)
US8999672B2 (en) * 2005-01-28 2015-04-07 James A. Williams Compositions and processes for improved plasmid DNA production
US10513711B2 (en) 2014-08-13 2019-12-24 Dupont Us Holding, Llc Genetic targeting in non-conventional yeast using an RNA-guided endonuclease
JP6823593B2 (ja) 2014-11-06 2021-02-03 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ Ｒｎａ誘導型エンドヌクレアーゼの細胞へのペプチド媒介輸送
EP4144844B1 (de) 2015-10-12 2025-09-10 DuPont US Holding, LLC Geschützte dna-schablonen zur genmodifizierung und erhöhten homologen rekombination in zellen und verfahren zur verwendung
SG11202100928QA (en) 2018-08-02 2021-02-25 Dyne Therapeutics Inc Muscle targeting complexes and uses thereof for treating facioscapulohumeral muscular dystrophy
CN120659627A (zh) 2022-07-29 2025-09-16 瑞泽恩制药公司 用于转铁蛋白受体（tfr）介导的脑和肌肉递送的组合物和方法
EP4612184A1 (de) 2022-11-04 2025-09-10 Regeneron Pharmaceuticals, Inc. An die calcium-spannungsabhängige kanalhilfsuntereinheit gamma 1 (cacng1) bindende proteine und cacng1-vermittelte abgabe an skelettmuskeln
KR20250116795A (ko) 2022-11-14 2025-08-01 리제너론 파마슈티칼스 인코포레이티드 별아교세포로의 섬유아세포 성장 인자 수용체 3-매개된 전달을 위한 조성물 및 방법

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4680265A (en) * 1984-09-25 1987-07-14 Eli Lilly And Company Method of selecting recombinant DNA-containing streptomyces
US5037746A (en) * 1986-12-03 1991-08-06 University Patents, Inc. RNA ribozyme polymerases, and methods
US5144019A (en) * 1989-06-21 1992-09-01 City Of Hope Ribozyme cleavage of HIV-I RNA

Also Published As

Publication number Publication date
EP0600877A1 (de) 1994-06-15
US5616459A (en) 1997-04-01
WO1992001806A1 (en) 1992-02-06
AU651380B2 (en) 1994-07-21
DE69131942T2 (de) 2000-06-29
DE69131942D1 (de) 2000-03-02
EP0600877B1 (de) 2000-01-26
CA2087519C (en) 2002-10-22
AU8517591A (en) 1992-02-18
EP0600877A4 (en) 1994-08-17
CA2087519A1 (en) 1992-01-17

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