DE69131942D1 - Auswahl von effizient ziel-rns spaltenden ribozymen - Google Patents

Auswahl von effizient ziel-rns spaltenden ribozymen

Info

Publication number
DE69131942D1
DE69131942D1 DE69131942T DE69131942T DE69131942D1 DE 69131942 D1 DE69131942 D1 DE 69131942D1 DE 69131942 T DE69131942 T DE 69131942T DE 69131942 T DE69131942 T DE 69131942T DE 69131942 D1 DE69131942 D1 DE 69131942D1
Authority
DE
Germany
Prior art keywords
ribozyms
cleaving
selection
efficient target
target rns
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69131942T
Other languages
English (en)
Other versions
DE69131942T2 (de
Inventor
Russell Kramer
David Dubnau
A Drlica
Abraham Pinter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Medicine And Dentistry Of Ne Us, University of
Original Assignee
Public Health Research Institute of City of New York Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Public Health Research Institute of City of New York Inc filed Critical Public Health Research Institute of City of New York Inc
Application granted granted Critical
Publication of DE69131942D1 publication Critical patent/DE69131942D1/de
Publication of DE69131942T2 publication Critical patent/DE69131942T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1041—Ribosome/Polysome display, e.g. SPERT, ARM
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/121—Hammerhead

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Operating, Guiding And Securing Of Roll- Type Closing Members (AREA)
  • Saccharide Compounds (AREA)
DE69131942T 1990-07-16 1991-07-16 Auswahl von effizient ziel-rns spaltenden ribozymen Expired - Lifetime DE69131942T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US55372990A 1990-07-16 1990-07-16
PCT/US1991/005017 WO1992001806A1 (en) 1990-07-16 1991-07-16 Selection of ribozymes that efficiently cleave target rna

Publications (2)

Publication Number Publication Date
DE69131942D1 true DE69131942D1 (de) 2000-03-02
DE69131942T2 DE69131942T2 (de) 2000-06-29

Family

ID=24210504

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69131942T Expired - Lifetime DE69131942T2 (de) 1990-07-16 1991-07-16 Auswahl von effizient ziel-rns spaltenden ribozymen

Country Status (7)

Country Link
US (1) US5616459A (de)
EP (1) EP0600877B1 (de)
AT (1) ATE189263T1 (de)
AU (1) AU651380B2 (de)
CA (1) CA2087519C (de)
DE (1) DE69131942T2 (de)
WO (1) WO1992001806A1 (de)

Families Citing this family (41)

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Publication number Priority date Publication date Assignee Title
US5496698A (en) * 1992-08-26 1996-03-05 Ribozyme Pharmaceuticals, Inc. Method of isolating ribozyme targets
US5693535A (en) * 1992-05-14 1997-12-02 Ribozyme Pharmaceuticals, Inc. HIV targeted ribozymes
US6017756A (en) * 1992-05-14 2000-01-25 Ribozyme Pharmaceuticals, Inc. Method and reagent for inhibiting hepatitis B virus replication
WO1994013793A1 (en) * 1992-12-04 1994-06-23 Apollon, Inc. Compounds and methods for the treatment of leukemias
FR2723371B1 (fr) * 1994-08-02 1996-10-31 Inst Nat Sante Rech Med Oligonucleotide reconnaissant une sequence d'acide nucleique a structure non lineaire, application a des fins therapeutiques et procede de preparation
JPH10503654A (ja) * 1994-08-05 1998-04-07 ワーナー−ランバート・コンパニー レトロウイルスの感染の治療におけるトランスドミナント・ネガティブ・レトロウイルス・インテグラーゼの使用方法
US6274369B1 (en) * 1996-02-02 2001-08-14 Invitrogen Corporation Method capable of increasing competency of bacterial cell transformation
EP0837142A1 (de) * 1996-10-15 1998-04-22 Smithkline Beecham Corporation Verfahren zum Nachweis essentieller Gene eines Erregers
US6139817A (en) * 1996-10-15 2000-10-31 Smithkline Beecham Corporation Method for determining gene essentiality in a pathogen
AU5927598A (en) 1997-01-23 1998-08-18 Immusol Incorporated Gene functional analysis and discovery using randomized or target-specific ribozyme gene vector libraries
CA2288640A1 (en) * 1997-05-09 1998-11-12 Ribozyme Pharmaceuticals, Inc. Enzymatic nucleic acids: synthesis, selection and use
US6548657B1 (en) 1997-06-09 2003-04-15 Ribozyme Pharmaceuticals, Inc. Method for screening nucleic acid catalysts
US6280936B1 (en) 1997-06-09 2001-08-28 Ribozyme Pharmaceuticals, Inc. Method for screening nucleic acid catalysts
US6183959B1 (en) 1997-07-03 2001-02-06 Ribozyme Pharmaceuticals, Inc. Method for target site selection and discovery
EP1007099A4 (de) * 1997-07-11 2004-11-24 Univ Brandeis Methode zur induktion von apoptose mittels senkung des thiamin-spiegels
EP1019429A4 (de) * 1997-08-20 2001-08-08 Somagenics Inc Antisense- und antigen-therapeutika mit verbesserten bindungseigenschaften und methoden zu ihrer anwendung
US6316612B1 (en) 1997-08-22 2001-11-13 Ribozyme Pharmaceuticals, Inc. Xylofuranosly-containing nucleoside phosphoramidites and polynucleotides
US6387703B1 (en) * 1997-10-07 2002-05-14 Smithkline Beecham Corporation Method for modulating gene expression
US6617438B1 (en) 1997-11-05 2003-09-09 Sirna Therapeutics, Inc. Oligoribonucleotides with enzymatic activity
US6127535A (en) 1997-11-05 2000-10-03 Ribozyme Pharmaceuticals, Inc. Nucleoside triphosphates and their incorporation into oligonucleotides
US6440695B1 (en) * 1998-04-17 2002-08-27 Whitehead Institute For Biomedical Research Method for producing diverse libraries of encoded polypeptides
DE19956568A1 (de) * 1999-01-30 2000-08-17 Roland Kreutzer Verfahren und Medikament zur Hemmung der Expression eines vorgegebenen Gens
GB9912965D0 (en) * 1999-06-03 1999-08-04 Oxford Biomedica Ltd In vivo selection method
US7829693B2 (en) * 1999-11-24 2010-11-09 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a target gene
US6541168B2 (en) * 2000-04-28 2003-04-01 Corning Incorporated Vacuum ultraviolet transmitting direct deposit vitrified silicon oxyfluoride lithography glass photomask blanks
US6808876B1 (en) * 2000-05-02 2004-10-26 Immusol, Inc. Cellular regulators of infectious agents and methods of use
US8546143B2 (en) 2001-01-09 2013-10-01 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a target gene
US20040002083A1 (en) * 2002-01-29 2004-01-01 Ye Ding Statistical algorithms for folding and target accessibility prediction and design of nucleic acids
EP1578767A4 (de) * 2002-12-04 2008-01-09 Algos Therapeutics Inc Verfahren und materialien zur modulation von trpm2
WO2004055198A2 (en) * 2002-12-12 2004-07-01 Chiron Corporation Device and method for in-line blood testing using biochips
US20050026290A1 (en) * 2003-08-01 2005-02-03 Ciardi Joseph Anthony Inhibiting gene expression with dsRNA
US20050176125A1 (en) * 2003-12-23 2005-08-11 Rosetta Inpharmatics Llc Alternatively spliced isoform of CDC25A phosphatase and methods of use
US20050266469A1 (en) * 2004-05-25 2005-12-01 Raymond Christopher K Alternatively spliced isoforms of checkpoint kinase 1 (CHK1)
US7150969B2 (en) * 2004-06-04 2006-12-19 Rosetta Inpharmatics Llc Alternatively spliced isoform of acetyl-CoA carboxylase 2 (ACC2)
US8999672B2 (en) * 2005-01-28 2015-04-07 James A. Williams Compositions and processes for improved plasmid DNA production
US10513711B2 (en) 2014-08-13 2019-12-24 Dupont Us Holding, Llc Genetic targeting in non-conventional yeast using an RNA-guided endonuclease
CN107406838A (zh) 2014-11-06 2017-11-28 纳幕尔杜邦公司 Rna引导的内切核酸酶向细胞中的肽介导的递送
DK3362560T3 (da) 2015-10-12 2022-11-07 Dupont Us Holding Llc Beskyttede dna-templater til genmodificering og forøget homolog rekombination i celler og fremgangsmåder til anvendelse
WO2024026474A1 (en) 2022-07-29 2024-02-01 Regeneron Pharmaceuticals, Inc. Compositions and methods for transferrin receptor (tfr)-mediated delivery to the brain and muscle
WO2024098002A1 (en) 2022-11-04 2024-05-10 Regeneron Pharmaceuticals, Inc. Calcium voltage-gated channel auxiliary subunit gamma 1 (cacng1) binding proteins and cacng1-mediated delivery to skeletal muscle
WO2024107765A2 (en) 2022-11-14 2024-05-23 Regeneron Pharmaceuticals, Inc. Compositions and methods for fibroblast growth factor receptor 3-mediated delivery to astrocytes

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4680265A (en) * 1984-09-25 1987-07-14 Eli Lilly And Company Method of selecting recombinant DNA-containing streptomyces
US5037746A (en) * 1986-12-03 1991-08-06 University Patents, Inc. RNA ribozyme polymerases, and methods
US5144019A (en) * 1989-06-21 1992-09-01 City Of Hope Ribozyme cleavage of HIV-I RNA

Also Published As

Publication number Publication date
EP0600877A1 (de) 1994-06-15
ATE189263T1 (de) 2000-02-15
EP0600877B1 (de) 2000-01-26
DE69131942T2 (de) 2000-06-29
AU651380B2 (en) 1994-07-21
CA2087519A1 (en) 1992-01-17
WO1992001806A1 (en) 1992-02-06
CA2087519C (en) 2002-10-22
AU8517591A (en) 1992-02-18
EP0600877A4 (en) 1994-08-17
US5616459A (en) 1997-04-01

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Owner name: THE UNIVERSITY OF MEDICINE AND DENTISTRY OF NE, US