ATE134218T1 - Methode zur reinigung rekombinanter t-pa von den ihren entsprechenden wirtszellproteinen - Google Patents

Methode zur reinigung rekombinanter t-pa von den ihren entsprechenden wirtszellproteinen

Info

Publication number
ATE134218T1
ATE134218T1 AT89900037T AT89900037T ATE134218T1 AT E134218 T1 ATE134218 T1 AT E134218T1 AT 89900037 T AT89900037 T AT 89900037T AT 89900037 T AT89900037 T AT 89900037T AT E134218 T1 ATE134218 T1 AT E134218T1
Authority
AT
Austria
Prior art keywords
host cell
cell proteins
corresponding host
purifying recombinant
disclosed
Prior art date
Application number
AT89900037T
Other languages
English (en)
Inventor
Vincent R Anicetti
Stuart E Builder
Billie J Marks
John R Ogez
Eric J Patzer
David A Vetterlein
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Application granted granted Critical
Publication of ATE134218T1 publication Critical patent/ATE134218T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT89900037T 1987-11-05 1988-11-02 Methode zur reinigung rekombinanter t-pa von den ihren entsprechenden wirtszellproteinen ATE134218T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/117,705 US5411864A (en) 1987-11-05 1987-11-05 Method of purifying recombinant proteins from corresponding host cell proteins

Publications (1)

Publication Number Publication Date
ATE134218T1 true ATE134218T1 (de) 1996-02-15

Family

ID=22374375

Family Applications (1)

Application Number Title Priority Date Filing Date
AT89900037T ATE134218T1 (de) 1987-11-05 1988-11-02 Methode zur reinigung rekombinanter t-pa von den ihren entsprechenden wirtszellproteinen

Country Status (6)

Country Link
US (1) US5411864A (de)
EP (1) EP0389538B1 (de)
JP (1) JP2756162B2 (de)
AT (1) ATE134218T1 (de)
DE (1) DE3855007T2 (de)
WO (1) WO1989004367A1 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0475447A3 (en) * 1990-09-14 1992-09-23 Fuji Photo Film Co Ltd Magnetically recordable photographic film, recording method and system therefor and camera-laboratory system
US7033774B2 (en) * 1997-04-21 2006-04-25 Glycozyme, Inc. Determination of recombinant glycosylated proteins and peptides in biological fluids
WO2001032915A2 (en) * 1999-11-04 2001-05-10 Genentech, Inc. Reversed-phase hplc assay for plasminogen activators
CN114426962B (zh) * 2021-12-21 2024-06-18 佛山汉腾生物科技有限公司 t-PA纯化方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3222084A1 (de) * 1982-06-11 1983-12-15 Bayer Ag, 5090 Leverkusen Monoklonale antikoerper
US4568488A (en) * 1984-01-11 1986-02-04 Lee Huang Sylvia Reverse immunoaffinity chromatography purification method
DE3663030D1 (en) * 1985-02-07 1989-06-01 Kowa Co Monoclonal antibodies to tissue plasminogen activator derived from human normal cells
JPS6229975A (ja) * 1985-08-01 1987-02-07 Mitsui Toatsu Chem Inc 粗ｔＰＡの精製法

Also Published As

Publication number Publication date
DE3855007T2 (de) 1996-09-19
EP0389538A1 (de) 1990-10-03
JP2756162B2 (ja) 1998-05-25
US5411864A (en) 1995-05-02
EP0389538B1 (de) 1996-02-14
JPH03500842A (ja) 1991-02-28
DE3855007D1 (de) 1996-03-28
WO1989004367A1 (en) 1989-05-18

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Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
EELA Cancelled due to lapse of time