AT77210B - Process for the preparation of a lipoid-like, nitrogen-containing compound from blood. - Google Patents

Process for the preparation of a lipoid-like, nitrogen-containing compound from blood.

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Publication number
AT77210B
AT77210B AT77210DA AT77210B AT 77210 B AT77210 B AT 77210B AT 77210D A AT77210D A AT 77210DA AT 77210 B AT77210 B AT 77210B
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AT
Austria
Prior art keywords
blood
lipoid
nitrogen
preparation
containing compound
Prior art date
Application number
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German (de)
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Chem Ind Basel
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Publication of AT77210B publication Critical patent/AT77210B/en

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  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Description

  

   <Desc/Clms Page number 1> 
 
 EMI1.1 
 



   In weiterer Ausbildung des durch das Stammpatent Nr. 73354 und dessem ersten Zusatzpatent Nr. 75374 geschützten Verfahrens wurde gefunden, dass man in dem Verfahren der Stammerfindung die dort zur Extraktion der beim Vermischen des eingeengten alkoholischen Auszuges mit Wasser erhaltenen Emulsion verwendetem chlorierten Kohlenwasserstoffe, wie Chloroform, ersetzen kann durch andere geeignete organische Lösungmittel, wie Äther, Benzol, Toluol, worauf man, wie in der   Stammernndang     beschrieben,   die wirksame Substanz fällt und reinigt. 



   Das Verfahren wird durch folgendes Beispiel erläutert :
Man lässt 40   l   Blut entweder in 4 kg einer einprozentigen Natriumoxalatlösung oder in 10   kg einer 26%igen MagnesiumsulfatJösung   unter Umrühren einlaufen und kocht diese Mischung am   Rückflusskühler   mit   120 l hochprozentigem Alkohol   während fünf Stunden. Hierauf wird der   Spritextrakt   bei zirka   70"0 nitriert.   Der in Sprit   unlösliche   Anteil wird leicht ausgepresst und nochmals mit 40   l   hochprozentigem Alkohol fünf Stunden in der angegebenen Weise gekocht. Es wird wiederum bei 700 C filtriert und der Rückstand scharf ausgepresst.

   Die Spritlösung wird sofort im hohen Vakuum und bei niedriger Temperatur eingedampft ; gegen das Ende der Destillation werden 10   1   destilliertes Wasser eingesaugt und die Destillation so geleitet, dass zum Schluss noch 16 bis   20 l Emulsion   verbleiben. Diese Emulsion wird dreimal nacheinander mit je 10 kg Äther je zirka zehn Minuten ausgerührt. Die erhaltene Ätherlösung wird auf zirka   4 l eingedampft   und nnter gutem Schütteln bzw. Rühren mit zirka   6 l   Aceton versetzt. Die hiebei entstandene Fällung wird abfiltriert, mit Äther ausgerührt und die Ätherlösung im Vakuum zur Trockne eingedampft. Das erhaltene Produkt ist identisch mit dem in der Stammerfindung beschriebenen. 



   An Stelle des in vorstehendem Beispiel angewendeten Äthers können zur Extraktion der Emulsion mit   ähnlichem   Erfolg auch andere organische   Lösungsmittel,   wie z. B. Benzol oder Toluol, verwendet werden. 

**WARNUNG** Ende DESC Feld kannt Anfang CLMS uberlappen**.



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   In a further development of the process protected by the parent patent No. 73354 and its first additional patent No. 75374, it was found that the chlorinated hydrocarbons used there for the extraction of the emulsion obtained when the concentrated alcoholic extract was mixed with water, such as chloroform, in the process of the parent invention , can be replaced by other suitable organic solvents such as ether, benzene, toluene, whereupon, as described in the Stammernndang, the active substance is precipitated and purified.



   The procedure is illustrated by the following example:
40 liters of blood are poured into either 4 kg of a 1% sodium oxalate solution or 10 kg of a 26% magnesium sulfate solution while stirring and this mixture is boiled on a reflux condenser with 120 liters of high percentage alcohol for five hours. The fuel extract is then nitrated at about 70 "0. The fraction that is insoluble in fuel is squeezed out slightly and boiled again with 40 liters of high-percentage alcohol for five hours in the manner indicated. It is again filtered at 700 ° C. and the residue is squeezed sharply.

   The fuel solution is immediately evaporated in a high vacuum and at low temperature; Towards the end of the distillation, 10 l of distilled water are sucked in and the distillation is directed in such a way that 16 to 20 l of emulsion remain at the end. This emulsion is stirred three times in succession with 10 kg of ether each time for about ten minutes. The ether solution obtained is evaporated to approx. 4 l and mixed with approx. 6 l of acetone while shaking or stirring well. The resulting precipitate is filtered off, stirred up with ether and the ether solution is evaporated to dryness in vacuo. The product obtained is identical to that described in the parent invention.



   Instead of the ether used in the above example, other organic solvents, such as. B. benzene or toluene can be used.

** WARNING ** End of DESC field may overlap beginning of CLMS **.

 

Claims (1)

PATENT-ANSPRUCH : Abänderung des durch Stammpatent Nr. 73354 und dessem ersten Znsatpatente Nr. 75374 geschützten Verfahrens zur Darstellung einer lipoidartigen stickstoffhaltigen Verbindung aus Blut, darin bestehend, dass man hier den aus mit Magnesiumsulfat oder Natriumoxalat vorbehandeltem oder frischem Blut erhaltenen Alkoholextrakt durch Eindampfen bei niedriger Temperatur vom grössten Teil des Alkohols befreit, den Destillationsrückstand mit Wasser vermischt, die erhaltene Emulsion statt mit chlorierten Kohlenwasserstoffen, wie Chloroform, mit anderen geeigneten organischen Lösungsmitteln, wie Äther, Benzol, EMI1.2 verarbeitet. * Erstes Zusatzpatent Nr. 75374. **WARNUNG** Ende CLMS Feld Kannt Anfang DESC uberlappen**. PATENT CLAIM: Modification of the process, protected by parent patent No. 73354 and its first Znsatpatent No. 75374, for the preparation of a lipoid-like nitrogen-containing compound from blood, consisting in that the alcohol extract obtained from fresh blood pretreated with magnesium sulfate or sodium oxalate or fresh blood is removed from the largest by evaporation at low temperature Part of the alcohol is freed, the distillation residue is mixed with water, the emulsion obtained, instead of with chlorinated hydrocarbons such as chloroform, with other suitable organic solvents such as ether, benzene, EMI1.2 processed. * First additional patent No. 75374. ** WARNING ** End of CLMS field may overlap beginning of DESC **.
AT77210D 1915-07-14 1917-04-26 Process for the preparation of a lipoid-like, nitrogen-containing compound from blood. AT77210B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AT73354T 1915-07-14
AT77210T 1917-04-26

Publications (1)

Publication Number Publication Date
AT77210B true AT77210B (en) 1919-07-25

Family

ID=25603736

Family Applications (1)

Application Number Title Priority Date Filing Date
AT77210D AT77210B (en) 1915-07-14 1917-04-26 Process for the preparation of a lipoid-like, nitrogen-containing compound from blood.

Country Status (1)

Country Link
AT (1) AT77210B (en)

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