AR124476A1 - Métodos para identificar y seleccionar plantas de maíz con el gen restaurador de la esterilidad masculina citoplasmática - Google Patents

Métodos para identificar y seleccionar plantas de maíz con el gen restaurador de la esterilidad masculina citoplasmática

Info

Publication number
AR124476A1
AR124476A1 ARP210103628A ARP210103628A AR124476A1 AR 124476 A1 AR124476 A1 AR 124476A1 AR P210103628 A ARP210103628 A AR P210103628A AR P210103628 A ARP210103628 A AR P210103628A AR 124476 A1 AR124476 A1 AR 124476A1
Authority
AR
Argentina
Prior art keywords
plant
locus
fragment
identifying
male sterility
Prior art date
Application number
ARP210103628A
Other languages
English (en)
Inventor
Monika Kloiber-Maitz
Carsten Knaak
Herv Dallocchio
Jean-Claude Castelle
Original Assignee
Kws Saat Se & Co Kgaa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kws Saat Se & Co Kgaa filed Critical Kws Saat Se & Co Kgaa
Publication of AR124476A1 publication Critical patent/AR124476A1/es

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
    • A01H1/045Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection using molecular markers
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4684Zea mays [maize]
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A01H1/022Genic fertility modification, e.g. apomixis
    • A01H1/023Male sterility
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289Male sterility
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/13Plant traits
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/172Haplotypes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Physiology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Mycology (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

La presente invención se relaciona con un método para identificar una planta o una parte de una planta, tal como una planta o parte de una planta de maíz, que comprende un novedoso locus restaurador de la fertilidad, en particular un locus restaurador de la fertilidad de la esterilidad masculina citoplasmática. La invención se relaciona además con marcadores moleculares asociados con dicho locus y con el uso de dichos marcadores en la identificación de plantas. La invención se relaciona además con métodos para generar plantas o partes de plantas que comprenden el novedoso locus restaurador de la fertilidad. Reivindicación 1: Un método para identificar una planta o parte de una planta de maíz, que comprende la detección de la presencia de (un haplotipo asociado con) un locus restaurador de la esterilidad masculina citoplasmática (fertilidad) en el cromosoma 3 (RF-03-01). Reivindicación 18: Un ácido polinucleico (aislado) que comprende uno o más (alelos del) marcador molecular de la Tabla 4 o de la Tabla 5, o el complemento o complemento inverso de dicho ácido polinucleico. Reivindicación 19: Un ácido polinucleico (aislado) que comprende o que consta de una o más de SEQ ID Nº 1, 5, 9, y 13, o un fragmento de las mismas, o una secuencia la cual es al menos 80%, con preferencia al menos 85%, con mayor preferencia al menos 90%, aún con mayor preferencia al menos 95% idéntica a una secuencia como se establece en cualquiera o más de SEQ ID Nº 1, 5, 9, y 13, o un fragmento de las mismas; o una o más de SEQ ID Nº 2, 6, 10, y 14, o un fragmento de las mismas, o una secuencia la cual es al menos 80%, con preferencia al menos 85%, con mayor preferencia al menos 90%, aún con mayor preferencia al menos 95% idéntica a una secuencia como se establece en cualquiera o más de SEQ ID Nº 2, 6, 10, y 14, o en un fragmento de las mismas. Reivindicación 28: Un método para generar una planta o parte de una planta de maíz, que comprende introducir en el genoma de dicha planta o parte de planta un locus como se define en cualquiera de las reivindicaciones 1 a 17 o un ácido polinucleico como se define en cualquiera de las reivindicaciones 18 a 27, o un fragmento (funcional) de los mismos.
ARP210103628A 2020-12-22 2021-12-22 Métodos para identificar y seleccionar plantas de maíz con el gen restaurador de la esterilidad masculina citoplasmática AR124476A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP20216347.3A EP4018821A1 (en) 2020-12-22 2020-12-22 Methods for identifying and selecting maize plants with cytoplasmatic male sterility restorer gene

Publications (1)

Publication Number Publication Date
AR124476A1 true AR124476A1 (es) 2023-03-29

Family

ID=73856729

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP210103628A AR124476A1 (es) 2020-12-22 2021-12-22 Métodos para identificar y seleccionar plantas de maíz con el gen restaurador de la esterilidad masculina citoplasmática

Country Status (7)

Country Link
US (1) US20240057538A1 (es)
EP (2) EP4018821A1 (es)
CN (1) CN116887669A (es)
AR (1) AR124476A1 (es)
CA (1) CA3202093A1 (es)
CL (1) CL2023001795A1 (es)
WO (1) WO2022136491A1 (es)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10117411B2 (en) 2010-10-06 2018-11-06 Dow Agrosciences Llc Maize cytoplasmic male sterility (CMS) C-type restorer RF4 gene, molecular markers and their use

Also Published As

Publication number Publication date
CA3202093A1 (en) 2022-06-30
US20240057538A1 (en) 2024-02-22
EP4266873A1 (en) 2023-11-01
WO2022136491A1 (en) 2022-06-30
EP4018821A1 (en) 2022-06-29
CN116887669A (zh) 2023-10-13
CL2023001795A1 (es) 2024-01-05

Similar Documents

Publication Publication Date Title
Ma et al. Origins of cultivars of Chrysanthemum—Evidence from the chloroplast genome and nuclear LFY gene
AR114025A1 (es) Trigo que comprende alelos restauradores de la fertilidad masculina
RU2013119981A (ru) Ген-восстановитель rf4 для цитоплазматической мужской стерильности (cms) c-типа кукурузы, молекулярные маркеры и их применение
Yan et al. The complete mitochondrial genome sequence of the western flower thrips Frankliniella occidentalis (Thysanoptera: Thripidae) contains triplicate putative control regions
AR077809A1 (es) Organismos homocigotas para una modificacion dirigida
AR050113A1 (es) Loci geneticos asociados con tolerancia a fusarium solani en soja
Zhang et al. The diploid origins of allopolyploid rose species studied using single nucleotide polymorphism haplotypes flanking a microsatellite repeat
AR104170A1 (es) Plantas con esterilidad masculina de la especie triticum
BRPI0819551B8 (pt) Processo de indentificação de uma planta de milho com tolerância à mancha foliar cinzenta
AR065282A1 (es) Composiciones y metodos que utilizan arn de transferencia de genes tipo cad para el control de nematodos
Celiński et al. Cross-species amplification and characterization of microsatellite loci in Pinus mugo Turra
AR124476A1 (es) Métodos para identificar y seleccionar plantas de maíz con el gen restaurador de la esterilidad masculina citoplasmática
Wang et al. Molecular sex identification in the hardy rubber tree (Eucommia ulmoides Oliver) via ddRAD markers
AR072830A1 (es) Marcadores moleculares ligados a la tolerancia a los inhibidores de ppo (protoporfirinogeno oxidasa) en la soja
KR101474284B1 (ko) 재래닭의 개체식별을 위한 마이크로새틀라이트 마커 및 이를 이용한 재래닭의 개체식별 방법
CO2019003924A2 (es) Plantas de crisantemo resistentes a la roya blanca
PL422535A1 (pl) Dwie pary oligonukleotydowych starterów do wykrywania obecności alleli dominującego lub recesywnego genu odporności na rdzę koronową Pc39 w genomie owsa zwyczajnego (Avena sativa L.), kombinacja dwóch par starterów oraz sposób wykrywania układu alleli genu Pc39
PE20230981A1 (es) Planta de stevia que tiene una relacion del contenido de rebaudiosido m elevada y metodo de eleccion para la misma
Seo et al. Construction of genetic linkage map using microsatellite and SNP markers in Korean native chicken
Song et al. Whole genome re-sequencing and development of SSR markers in oriental melon
KURABAYASHI et al. Partial mitochondrial genome organization of the heterostrophan gastropod Omalogyra atomus and its systematic significance
CL2023000133A1 (es) Gen que conduce a resistencia al virus de la fruta rugosa café del tomate (tobrfv)
UY38693A (es) Tolerancia a la sequía en maíz
CO2018004683A2 (es) Plantas de crisantemo resistentes a la roya blanca
AR087718A1 (es) Marcadores moleculares asociados con la resistencia al cancro del tallo de la soya