AR117781A1 - HEAT RESISTANT ISOAMYLASE - Google Patents

HEAT RESISTANT ISOAMYLASE

Info

Publication number
AR117781A1
AR117781A1 ARP200100047A ARP200100047A AR117781A1 AR 117781 A1 AR117781 A1 AR 117781A1 AR P200100047 A ARP200100047 A AR P200100047A AR P200100047 A ARP200100047 A AR P200100047A AR 117781 A1 AR117781 A1 AR 117781A1
Authority
AR
Argentina
Prior art keywords
isoamylase
amino acid
acid sequence
seq
heat resistant
Prior art date
Application number
ARP200100047A
Other languages
Spanish (es)
Inventor
Jun Yoshikawa
Ryoko Sano
Masahiro Baba
Original Assignee
Godo Shusei Kk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Godo Shusei Kk filed Critical Godo Shusei Kk
Publication of AR117781A1 publication Critical patent/AR117781A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material

Abstract

Una isoamilasa con resistencia al calor mejorada y un método para elaborar la isoamilasa. Una isoamilasa que comprende una secuencia de aminoácidos representada por SEQ ID Nº 1, o una isoamilasa que comprende una secuencia de aminoácidos en la que de uno a varios residuos de aminoácidos se eliminan, sustituyen o insertan en la secuencia de aminoácidos representada por SEQ ID Nº 1, y que tiene al menos 80% de identidad de secuencia con la secuencia de aminoácidos representada por SEQ ID Nº 1, en donde la isoamilasa tiene al menos una mutación de aminoácido seleccionada del grupo que consiste en D268S, A241T y M574V. Reivindicación 10: Una composición enzimática para sacarificar almidón, caracterizada porque comprende la isoamilasa de acuerdo con cualquiera de las reivindicaciones 1 a 5.An isoamylase with improved heat resistance and a method for making isoamylase. An isoamylase comprising an amino acid sequence represented by SEQ ID No. 1, or an isoamylase comprising an amino acid sequence in which one to several amino acid residues are deleted, substituted or inserted in the amino acid sequence represented by SEQ ID No. 1, and having at least 80% sequence identity with the amino acid sequence represented by SEQ ID No. 1, wherein the isoamylase has at least one amino acid mutation selected from the group consisting of D268S, A241T, and M574V. Claim 10: An enzymatic composition for saccharifying starch, characterized in that it comprises the isoamylase according to any of claims 1 to 5.

ARP200100047A 2019-01-09 2020-01-08 HEAT RESISTANT ISOAMYLASE AR117781A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2019002159 2019-01-09

Publications (1)

Publication Number Publication Date
AR117781A1 true AR117781A1 (en) 2021-08-25

Family

ID=71521675

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP200100047A AR117781A1 (en) 2019-01-09 2020-01-08 HEAT RESISTANT ISOAMYLASE

Country Status (3)

Country Link
JP (1) JP7433246B2 (en)
AR (1) AR117781A1 (en)
WO (1) WO2020145288A1 (en)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69939829D1 (en) 1998-07-02 2008-12-11 Novozymes As STRENGTH "DEBRANCHING ENZYME"
CN109153984A (en) 2016-05-27 2019-01-04 合同酒精株式会社 Heat resistance isoamylase

Also Published As

Publication number Publication date
JP7433246B2 (en) 2024-02-19
WO2020145288A1 (en) 2020-07-16
JPWO2020145288A1 (en) 2021-11-25

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