AR093691A1 - BIOSINTETIC GEN-UK-2 AND METHOD TO IMPROVE THE PRODUCTIVITY OF UK-2 USING THE GEN - Google Patents
BIOSINTETIC GEN-UK-2 AND METHOD TO IMPROVE THE PRODUCTIVITY OF UK-2 USING THE GENInfo
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- AR093691A1 AR093691A1 ARP130104438A ARP130104438A AR093691A1 AR 093691 A1 AR093691 A1 AR 093691A1 AR P130104438 A ARP130104438 A AR P130104438A AR P130104438 A ARP130104438 A AR P130104438A AR 093691 A1 AR093691 A1 AR 093691A1
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- cluster
- region
- productivity
- biosynthetic genes
- gen
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Un método de producción capaz de producir en forma masiva UK-2 a bajo costo, el ADN genómico del Streptoverticillium sp. 3-7, que produce UK-2, fue analizado para identificar una región que se estima es un racimo de genes biosintéticos UK-2. Además mediante hibridación por colonia, se pudieron aislar con éxito ADNs en la región. Además se usaron los ADNs para preparar una cepa en la cual se interrumpieron los genes presentes en la región. Se descubrió que la cepa no produce UK-2. Se verificó que la región genómica era el racimo de genes biosintéticos UK-2. Asimismo, se transformó el Streptoverticillium sp. 3-7 mediante la introducción de un vector en el cual se insertó el racimo de genes biosintéticos UK-2 aislados. También se descubrió que la productividad de UK-2 mediante el transformante se mejoró entre 10 y 60 veces o más en comparación con la de la cepa parenteral. Además se reveló que en cada célula de estos transformantes estaban presentes 2 copias del racimo de genes biosintéticos de UK-2 respectivamente. Reivindicación 5: Un método para determinar la productividad de UK-2, caracterizado porque comprende la detección, en una bacteria de prueba, de la presencia de un ácido nucleico que comprende una secuencia base del ácido nucleico según cualquiera de las reivindicaciones 1 a 3 o una secuencia base complementaria a la secuencia.A production method capable of mass-producing UK-2 at low cost, the genomic DNA of Streptoverticillium sp. 3-7, which produces UK-2, was analyzed to identify a region that is estimated to be a cluster of UK-2 biosynthetic genes. In addition, by colony hybridization, DNAs in the region could be successfully isolated. In addition, the DNAs were used to prepare a strain in which the genes present in the region were disrupted. It was discovered that the strain does not produce UK-2. It was verified that the genomic region was the cluster of biosynthetic genes UK-2. Likewise, Streptoverticillium sp. 3-7 by introducing a vector into which the cluster of isolated UK-2 biosynthetic genes was inserted. It was also found that the productivity of UK-2 by the transformant was improved between 10 and 60 times or more compared to that of the parenteral strain. It was also revealed that in each cell of these transformants, 2 copies of the cluster of biosynthetic genes of UK-2 were present respectively. Claim 5: A method for determining the productivity of UK-2, characterized in that it comprises the detection, in a test bacterium, of the presence of a nucleic acid comprising a nucleic acid base sequence according to any one of claims 1 to 3 or a base sequence complementary to the sequence.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ARP130104438A AR093691A1 (en) | 2013-11-29 | 2013-11-29 | BIOSINTETIC GEN-UK-2 AND METHOD TO IMPROVE THE PRODUCTIVITY OF UK-2 USING THE GEN |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ARP130104438A AR093691A1 (en) | 2013-11-29 | 2013-11-29 | BIOSINTETIC GEN-UK-2 AND METHOD TO IMPROVE THE PRODUCTIVITY OF UK-2 USING THE GEN |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR093691A1 true AR093691A1 (en) | 2015-06-17 |
Family
ID=56117106
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP130104438A AR093691A1 (en) | 2013-11-29 | 2013-11-29 | BIOSINTETIC GEN-UK-2 AND METHOD TO IMPROVE THE PRODUCTIVITY OF UK-2 USING THE GEN |
Country Status (1)
| Country | Link |
|---|---|
| AR (1) | AR093691A1 (en) |
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2013
- 2013-11-29 AR ARP130104438A patent/AR093691A1/en active IP Right Grant
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