AR088699A1 - DIACILGLICEROL LIPASA AND USES OF THE SAME - Google Patents

DIACILGLICEROL LIPASA AND USES OF THE SAME

Info

Publication number
AR088699A1
AR088699A1 ARP120104200A AR088699A1 AR 088699 A1 AR088699 A1 AR 088699A1 AR P120104200 A ARP120104200 A AR P120104200A AR 088699 A1 AR088699 A1 AR 088699A1
Authority
AR
Argentina
Prior art keywords
lipase
dag
methods
refers
sequence
Prior art date
Application number
Other languages
Spanish (es)
Inventor
Capdevielle Joel
Ferran Edgardo
Ferrara Pascual
Guillemot Jean
Claude-Legoux Richard
Vermat Thierry
Original Assignee
Sanofi Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanofi Sa filed Critical Sanofi Sa
Publication of AR088699A1 publication Critical patent/AR088699A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
    • G01N2333/918—Carboxylic ester hydrolases (3.1.1)

Abstract

Se ha encontrado que la proteína ABHD11 exhibe actividad diacilglicerol lipasa a condición de que la secuencia de orientación mitocondrial N-terminal esté ausente. La presente se refiere por tanto a una diacilglicerol (DAG) lipasa purificada y enzimáticamente activa que comprende una secuencia al menos un 50% idéntica a la SEQ ID Nº 2, 3 ó 4, o una secuencia consistente en un fragmento de al menos 50 aminoácidos consecutivos de SEQ ID Nº 2, 3 ó 4, en la que dicha DAG lipasa es capaz de catalizar la hidrólisis de 1-estearoil-2-araquidonoil-sn-glicerol a 2-araquidonoilglicerol. Se refiere también a ácidos nucleicos que codifican dicha DAG lipasa, así como a vectores de expresión y células hospedadoras relacionados. La presente se refiere adicionalmente a métodos para producir dicha DAG lipasa, a métodos para cribar moduladores de dicha DAG lipasa y a métodos para ensayar la calidad de una composición que comprende un inhibidor de dicha DAG lipasa.ABHD11 protein has been found to exhibit diacylglycerol lipase activity provided that the N-terminal mitochondrial targeting sequence is absent. This refers therefore to a purified and enzymatically active diacylglycerol (DAG) lipase comprising a sequence at least 50% identical to SEQ ID No. 2, 3 or 4, or a sequence consisting of a fragment of at least 50 amino acids consecutive of SEQ ID No. 2, 3 or 4, wherein said DAG lipase is capable of catalyzing the hydrolysis of 1-stearoyl-2-arachidonoyl-sn-glycerol to 2-arachidonoyl glycerol. It also refers to nucleic acids encoding said DAG lipase, as well as to expression vectors and related host cells. This refers further to methods for producing said DAG lipase, to methods for screening modulators of said DAG lipase and to methods for testing the quality of a composition comprising an inhibitor of said DAG lipase.

ARP120104200 2011-11-09 2012-11-08 DIACILGLICEROL LIPASA AND USES OF THE SAME AR088699A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP11306462 2011-11-09

Publications (1)

Publication Number Publication Date
AR088699A1 true AR088699A1 (en) 2014-06-25

Family

ID=47143117

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP120104200 AR088699A1 (en) 2011-11-09 2012-11-08 DIACILGLICEROL LIPASA AND USES OF THE SAME

Country Status (2)

Country Link
AR (1) AR088699A1 (en)
WO (1) WO2013068445A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3629021A1 (en) * 2018-09-26 2020-04-01 Euroimmun Medizinische Labordiagnostika AG Diagnosis of a neuroautoimmune disease

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2597114B1 (en) 1986-04-10 1994-02-25 Sanofi MICROBIOLOGICAL PROCESS FOR OBTAINING PROTEIN BY CULTURING A MUTANT BACTERIAL STRAIN
US5407822A (en) 1991-10-02 1995-04-18 Sanofi Artificial promoter for the expression of proteins in yeast
CA2350388A1 (en) * 1998-11-12 2000-05-18 Incyte Pharmaceuticals, Inc. Human hydrolase proteins
AU3395900A (en) * 1999-03-12 2000-10-04 Human Genome Sciences, Inc. Human lung cancer associated gene sequences and polypeptides
WO2001055325A2 (en) * 2000-01-31 2001-08-02 Human Genome Sciences, Inc. Nucleic acids, proteins, and antibodies
US6500657B1 (en) 2000-03-31 2002-12-31 Millennium Pharmaceuticals, Inc. 33167, a novel human hydrolase and uses therefor
WO2003023355A2 (en) * 2001-09-06 2003-03-20 The Burnham Institute Serine/threonine hydrolase proteins and screening assays
JP2013505232A (en) 2009-09-16 2013-02-14 スティヒティング ヘット ネーデルラント カンカー インスティテュート Fra-1 target gene as a drug target for cancer therapy

Also Published As

Publication number Publication date
WO2013068445A1 (en) 2013-05-16

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