AR070322A1 - PROCEDURE FOR THE QUANTIFICATION OF ANTIGEN NS1 OF DENGUE IN IMMUNOCROMATOGRAPHIC STRIP - Google Patents

PROCEDURE FOR THE QUANTIFICATION OF ANTIGEN NS1 OF DENGUE IN IMMUNOCROMATOGRAPHIC STRIP

Info

Publication number
AR070322A1
AR070322A1 ARP090100289A ARP090100289A AR070322A1 AR 070322 A1 AR070322 A1 AR 070322A1 AR P090100289 A ARP090100289 A AR P090100289A AR P090100289 A ARP090100289 A AR P090100289A AR 070322 A1 AR070322 A1 AR 070322A1
Authority
AR
Argentina
Prior art keywords
antigen
dengue
quantification
deposited
procedure
Prior art date
Application number
ARP090100289A
Other languages
Spanish (es)
Inventor
Nieto Gerardo E Guillen
Perez Gilda Lemos
De Cossio Dorta Duque Jorge Fernandez
Masso Julio Raul Fernandez
Argudin Tamara Diaz
Perez Lidia Ines Novoa
Gardon Daniel Octavio Palenzuela
Original Assignee
Ct Ingenieria Genetica Biotech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ct Ingenieria Genetica Biotech filed Critical Ct Ingenieria Genetica Biotech
Publication of AR070322A1 publication Critical patent/AR070322A1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/18—Togaviridae; Flaviviridae
    • G01N2333/183—Flaviviridae, e.g. pestivirus, mucosal disease virus, bovine viral diarrhoea virus, classical swine fever virus (hog cholera virus) or border disease virus
    • G01N2333/185—Flaviviruses or Group B arboviruses, e.g. yellow fever virus, japanese encephalitis, tick-borne encephalitis, dengue

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Procedimiento no instrumental de un solo paso para la cuantificacion del antígeno de dengue NS1 en fase aguda temprana, presente en muestras de suero, plasma o sangre, a través de su reaccion con un anticuerpo específico monoclonal y/o policlonal contra el antígeno conjugados a oro coloidal, que se encuentra depositado sobre una matriz de la que es liberado al ponerse en contacto con la muestra. Este inmunocomplejo migra a través de un soporte solido y es atrapado al interactuar con otros anticuerpos específicos contra epítopes del antígeno, para los cuatro serotipos del virus, que se encuentran firmemente unidos a una zona reactiva del soporte. El inmunocomplejo se deposita en el área de reaccion, que se evidencia por la aparicion de una marca visualmente detectable que segun el color, indica un resultado positivo a la presencia del antígeno NS1 y por tanto una infeccion por virus de dengue.Non-instrumental single-step procedure for quantification of the dengue NS1 antigen in early acute phase, present in serum, plasma or blood samples, through its reaction with a specific monoclonal and / or polyclonal antibody against the gold-conjugated antigen colloidal, which is deposited on a matrix from which it is released by contacting the sample. This immune complex migrates through a solid support and is trapped by interacting with other specific antibodies against epitopes of the antigen, for the four serotypes of the virus, which are firmly attached to a reactive zone of the support. The immunocomplex is deposited in the reaction area, which is evidenced by the appearance of a visually detectable brand that according to color, indicates a positive result to the presence of the NS1 antigen and therefore a dengue virus infection.

ARP090100289A 2008-01-30 2009-01-29 PROCEDURE FOR THE QUANTIFICATION OF ANTIGEN NS1 OF DENGUE IN IMMUNOCROMATOGRAPHIC STRIP AR070322A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CU20080015 2008-01-30

Publications (1)

Publication Number Publication Date
AR070322A1 true AR070322A1 (en) 2010-03-31

Family

ID=40913322

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP090100289A AR070322A1 (en) 2008-01-30 2009-01-29 PROCEDURE FOR THE QUANTIFICATION OF ANTIGEN NS1 OF DENGUE IN IMMUNOCROMATOGRAPHIC STRIP

Country Status (2)

Country Link
AR (1) AR070322A1 (en)
WO (1) WO2009094955A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102393454A (en) * 2011-08-24 2012-03-28 中国检验检疫科学研究院 Colloidal gold test strip for detecting dengue virus antibody and preparation method and application thereof

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2794864A1 (en) * 1999-06-09 2000-12-15 Pasteur Institut Early detection of flavivirus infection, especially dengue fever, comprises detecting non-structural glycoprotein (NS1) in a sandwich assay using antibodies specific for the hexameric protein form
DE102006000305A1 (en) * 2005-06-22 2007-01-04 Mahidol University Method for predicting dengue virus infection and development of hemorrhagic fever, comprises detecting both the viral non-structural protein 1 and terminal complement complexes

Also Published As

Publication number Publication date
WO2009094955A2 (en) 2009-08-06
WO2009094955A3 (en) 2009-11-12

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