WO2009094955A3 - Method for quantification of the dengue ns1 antigen on an immunochromatographic strip - Google Patents

Method for quantification of the dengue ns1 antigen on an immunochromatographic strip Download PDF

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Publication number
WO2009094955A3
WO2009094955A3 PCT/CU2009/000001 CU2009000001W WO2009094955A3 WO 2009094955 A3 WO2009094955 A3 WO 2009094955A3 CU 2009000001 W CU2009000001 W CU 2009000001W WO 2009094955 A3 WO2009094955 A3 WO 2009094955A3
Authority
WO
WIPO (PCT)
Prior art keywords
antigen
dengue
quantification
immune complex
deposited
Prior art date
Application number
PCT/CU2009/000001
Other languages
Spanish (es)
French (fr)
Other versions
WO2009094955A2 (en
Inventor
Gilda LEMOS PÉREZ
María Elena FERNÁNDEZ DE COSSIO DORTA-DUQUE
Tamara DÍAZ ARGUDÍN
Julio Raúl FERNÁNDEZ MASSÓ
Daniel Octavio Palenzuela Gardon
Lidia Inés NOVOA PÉREZ
Gerardo Enrique GUILLÉN NIETO
Original Assignee
Centro De Ingenieria Genetica Y Biotecnologia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centro De Ingenieria Genetica Y Biotecnologia filed Critical Centro De Ingenieria Genetica Y Biotecnologia
Publication of WO2009094955A2 publication Critical patent/WO2009094955A2/en
Publication of WO2009094955A3 publication Critical patent/WO2009094955A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/18—Togaviridae; Flaviviridae
    • G01N2333/183—Flaviviridae, e.g. pestivirus, mucosal disease virus, bovine viral diarrhoea virus, classical swine fever virus (hog cholera virus) or border disease virus
    • G01N2333/185—Flaviviruses or Group B arboviruses, e.g. yellow fever virus, japanese encephalitis, tick-borne encephalitis, dengue

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention relates to one-step instrument-free method for the quantification of the dengue NS1 antigen in early acute phase, present in serum, plasma and blood samples, by reacting same with a specific monoclonal and/or polyclonal antibody against the antigen, which antibodies are conjugated to colloidal gold deposited on a matrix from which it is released upon contact with the sample. This immune complex migrates through a solid medium and is trapped upon interaction with other specific antibodies against epitopes of the antigen for the four serotypes of the virus which are firmly bound to a reactive zone of the medium. The immune complex is deposited in the reaction area which is manifested by the appearance of a visually detectable mark which depending on the colour thereof indicates a positive result in response to the presence of the NS1 antigen and consequently a dengue virus infection.
PCT/CU2009/000001 2008-01-30 2009-01-30 Method for quantification of the dengue ns1 antigen on an immunochromatographic strip WO2009094955A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CU2008-0015 2008-01-30
CU20080015 2008-01-30

Publications (2)

Publication Number Publication Date
WO2009094955A2 WO2009094955A2 (en) 2009-08-06
WO2009094955A3 true WO2009094955A3 (en) 2009-11-12

Family

ID=40913322

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CU2009/000001 WO2009094955A2 (en) 2008-01-30 2009-01-30 Method for quantification of the dengue ns1 antigen on an immunochromatographic strip

Country Status (2)

Country Link
AR (1) AR070322A1 (en)
WO (1) WO2009094955A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102393454A (en) * 2011-08-24 2012-03-28 中国检验检疫科学研究院 Colloidal gold test strip for detecting dengue virus antibody and preparation method and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2794864A1 (en) * 1999-06-09 2000-12-15 Pasteur Institut Early detection of flavivirus infection, especially dengue fever, comprises detecting non-structural glycoprotein (NS1) in a sandwich assay using antibodies specific for the hexameric protein form
DE102006000305A1 (en) * 2005-06-22 2007-01-04 Mahidol University Method for predicting dengue virus infection and development of hemorrhagic fever, comprises detecting both the viral non-structural protein 1 and terminal complement complexes

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2794864A1 (en) * 1999-06-09 2000-12-15 Pasteur Institut Early detection of flavivirus infection, especially dengue fever, comprises detecting non-structural glycoprotein (NS1) in a sandwich assay using antibodies specific for the hexameric protein form
DE102006000305A1 (en) * 2005-06-22 2007-01-04 Mahidol University Method for predicting dengue virus infection and development of hemorrhagic fever, comprises detecting both the viral non-structural protein 1 and terminal complement complexes

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ANONYMOUS: "Dengue NS1 Ag strip 70700", INTERNET ARTICLE - BIORAD, June 2007 (2007-06-01), XP002544468, Retrieved from the Internet <URL:http://www3.bio-rad.com/cmc_upload/Literature/inserts/70700_880094_GB.pdf> [retrieved on 20090907] *
DUSSART PHILIPPE; PETIT LAURE; LABEAU BHETY; BREMAND LAETITIA; LEDUC ALEXANDRE; MOUA DAVID; MATHEUS SÉVERINE; BARIL LAURENCE: "Evaluation of Two New Commercial Tests for the Diagnosis of Acute Dengue Virus Infection Using NS1 Antigen Detection in Human Serum", PLOS NEGLECTED TROPICAL DISEASES,, vol. 2, no. 8, 1 August 2008 (2008-08-01), pages E280-1 - 280-9, XP009122311 *

Also Published As

Publication number Publication date
WO2009094955A2 (en) 2009-08-06
AR070322A1 (en) 2010-03-31

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