AR050294A1 - Reduccion de zeina en semillas de maiz transgenico - Google Patents

Reduccion de zeina en semillas de maiz transgenico

Info

Publication number
AR050294A1
AR050294A1 ARP050103386A AR050294A1 AR 050294 A1 AR050294 A1 AR 050294A1 AR P050103386 A ARP050103386 A AR P050103386A AR 050294 A1 AR050294 A1 AR 050294A1
Authority
AR
Argentina
Prior art keywords
dna
oriented
sense
plant
reading
Prior art date
Application number
Other languages
English (en)
Inventor
Thomas Malvar
Shihshieh Huang
Michael Hans Luethy
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of AR050294A1 publication Critical patent/AR050294A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254—Tryptophan or lysine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Se producen agentes de suspension genética que comprenden ARN orientado en el sentido opuesto del marco de lectura, bajo la forma de un rizo de ARN antisentido, en células de organismos transgénicos, por ejemplo, plantas, mediante la transcripcion de una construccion de ADN recombinante que comprende, en el orden de 5' a 3', un elemento promotor unido operativamente a más de un ADN orientado en el sentido opuesto del marco de lectura y uno o más elementos de ADN complementarios. Reivindicacion 1:Una construccion de ADN recombinante para suprimir una pluralidad de genes blanco de zeína de maíz caracterizada porque comprende, en le orden de 5' a 3', un elemento promotor unido operativamente a una pluralidad de ADN orientados en el sentido opuesto del marco de lectura de una pluralidad de genes blanco de zeína de maíz, donde los ADN antisentido se designan, en el ordén de 5' a 3', elemento 1, elemento 2, y una pluralidad de elementos de ADN orientados en el sentido del marco de lectura de una pluralidad de elementos de ADN orientados en el sentido del marco de lectura de una pluralidad de genes blanco de zeína de maíz, designados en el orden de 5' a 3', elemento 3, elemento 4, donde el ADN antisentido 2 tiene al menos una porcion de su secuencia en su extremo 3' que no es complementaria consigo misma o con el elemento de ADN orientado en el sentido del marco de lectura3, y donde el ARN orientado en el sentido del marco de lectura transcripto por elementos de ADN orientados en el sentido del marco de lectura es complementario con el extremo 5' del ARN antisentido transcripto por los ADN antisentido. Reivindicacion 9: Un método para generar semillas de maíz que tiene al menos una característica seleccionada entre lisina mejorada, aceite mejorado y triptofano mejorado, caracterizado porque comprende los pasos de: a) transformar una célula vegetal con la construccion de ADN recombinante de la reivindicacion 1 o la reivindicacion 4 para suprimir más de un gen blanco de zeína de maíz; y b) regenerar la célula vegetal en una planta transgénica fértil, donde la planta contiene la construccion en su genoma; y c) cosechar semillas de la planta, donde las semillas tienen al menos una característica seleccionada entre lisina mejorada, aceite mejorado y triptofano mejorado respecto de las semillas de la misma variedad no transformadas con la construccion. Reivindicacion 11: Una planta transgénica caracterizada porque comprende en su genoma una construccion de ADN de la reivindicacion 1 o la reivindicacion 4 para suprimir más de un gen blanco de zeína de maíz. Reivindicacion 12: Una semilla caracterizada porque ha sido cosechada de una planta de reivindicacion 11. Reivindicacion 14: Un producto procesado de la semilla de la reivindicacion 12, caracterizado porque el producto es un alimento, una racion o una composicion de proteínas parcialmente purificadas.
ARP050103386 2004-08-11 2005-08-12 Reduccion de zeina en semillas de maiz transgenico AR050294A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US60085904P 2004-08-11 2004-08-11
US11/057,062 US7855323B2 (en) 2004-02-10 2005-02-10 Recombinant DNA for gene suppression

Publications (1)

Publication Number Publication Date
AR050294A1 true AR050294A1 (es) 2006-10-11

Family

ID=37075568

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP050103386 AR050294A1 (es) 2004-08-11 2005-08-12 Reduccion de zeina en semillas de maiz transgenico

Country Status (6)

Country Link
US (3) US7855323B2 (es)
EP (1) EP1799833A4 (es)
AR (1) AR050294A1 (es)
BR (1) BRPI0514213A (es)
CA (1) CA2583501A1 (es)
WO (1) WO2007024207A1 (es)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7741533B2 (en) 2000-11-07 2010-06-22 Pioneer Hi-Bred International, Inc. Grain quality through altered expression of seed proteins
US7566813B2 (en) 2002-03-21 2009-07-28 Monsanto Technology, L.L.C. Nucleic acid constructs and methods for producing altered seed oil compositions
AR047598A1 (es) * 2004-02-10 2006-01-25 Monsanto Technology Llc Semilla de maiz transgenica con mayor contenido de aminoacidos
US7683237B2 (en) * 2004-02-10 2010-03-23 Monsanto Technology Llc Maize seed with synergistically enhanced lysine content
US20060075515A1 (en) * 2004-08-11 2006-04-06 Luethy Michael H Enhanced zein reduction in transgenic corn seed
US7855323B2 (en) 2004-02-10 2010-12-21 Monsanto Technology Llc Recombinant DNA for gene suppression
US8481306B2 (en) * 2004-12-09 2013-07-09 Monsanto Technology Llc Methods for ligation independent cloning of DNA
US20060150286A1 (en) * 2004-12-23 2006-07-06 Shihshieh Huang Gene suppression in transgenic plants using multiple constructs
US20060242736A1 (en) * 2004-12-23 2006-10-26 Shihshieh Huang Dissimilar promoters for gene suppression
CA2592894C (en) * 2004-12-28 2013-08-27 Pioneer Hi-Bred International, Inc. Improved grain quality through altered expression of gamma-zein protein
BRPI0616848A8 (pt) * 2005-10-03 2017-09-19 Monsanto Technology Llc Semente de planta transgênica com maior teor de lisina
MX2008011625A (es) 2006-03-10 2008-11-14 Monsanto Technology Llc Semilla y composiciones de aceite de soya y metodos para hacer los mismos.
ES2655700T3 (es) 2006-08-31 2018-02-21 Monsanto Technology, Llc ARN pequeños en fase
US8097712B2 (en) 2007-11-07 2012-01-17 Beelogics Inc. Compositions for conferring tolerance to viral disease in social insects, and the use thereof
WO2010117737A1 (en) * 2009-03-30 2010-10-14 Monsanto Technology Llc Rice transgenic event17053 and methods of use thereof
WO2010117735A1 (en) * 2009-03-30 2010-10-14 Monsanto Technology Llc Transgenic rice event17314 and methods of use thereof
US8962584B2 (en) 2009-10-14 2015-02-24 Yissum Research Development Company Of The Hebrew University Of Jerusalem, Ltd. Compositions for controlling Varroa mites in bees
BR122019026534B1 (pt) 2012-06-26 2022-07-19 Monsanto Technology Llc Método para selecionar uma planta alfafa com componentes de valor alimentar melhorados de uma população de plantas alfafa
MX359191B (es) 2013-07-19 2018-09-18 Monsanto Technology Llc Composiciones y métodos para controlar leptinotarsa.
EP3125676A4 (en) 2014-04-01 2018-02-14 Monsanto Technology LLC Compositions and methods for controlling insect pests
EP3174982A4 (en) 2014-07-29 2018-06-20 Monsanto Technology LLC Compositions and methods for controlling insect pests
RU2723049C2 (ru) 2015-01-22 2020-06-08 Монсанто Текнолоджи Ллс Композиции и способы борьбы с leptinotarsa
CA3011521A1 (en) 2016-01-26 2017-08-03 Monsanto Technology Llc Compositions and methods for controlling insect pests

Family Cites Families (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5428147A (en) * 1983-04-15 1995-06-27 Mycogen Plant Science, Inc. Octopine T-DNA promoters
US5453566A (en) * 1986-03-28 1995-09-26 Calgene, Inc. Antisense regulation of gene expression in plant/cells
US5107065A (en) * 1986-03-28 1992-04-21 Calgene, Inc. Anti-sense regulation of gene expression in plant cells
US5258300A (en) 1988-06-09 1993-11-02 Molecular Genetics Research And Development Limited Partnership Method of inducing lysine overproduction in plants
US5231020A (en) * 1989-03-30 1993-07-27 Dna Plant Technology Corporation Genetic engineering of novel plant phenotypes
US5082993A (en) 1989-08-01 1992-01-21 Orsan High-lysine corn
DE3935473A1 (de) 1989-10-25 1991-05-02 Hoechst Ag Rna mit endonuclease- und antisense-aktivitaet, ihre herstellung und ihre verwendung
HUT57265A (en) 1989-11-03 1991-11-28 Zaadunie Bv Process for producing plants of diminished infection-sensitivity
US6777589B1 (en) 1990-01-22 2004-08-17 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
JP3209744B2 (ja) * 1990-01-22 2001-09-17 デカルブ・ジェネティクス・コーポレーション 結実能力のある遺伝子変換コーン
US6329574B1 (en) 1990-01-22 2001-12-11 Dekalb Genetics Corporation High lysine fertile transgenic corn plants
GB9201371D0 (en) 1992-01-22 1992-03-11 Enzymatix Ltd Crystallisation process
US5773691A (en) 1992-03-19 1998-06-30 E. I. Du Pont De Nemours And Company Chimeric genes and methods for increasing the lysine and threonine content of the seeds of plants
EP1394257B1 (en) 1992-03-19 2007-08-08 E.I. Dupont De Nemours And Company Nucleic acid fragments and methods for increasing the lysine and threonine content of the seeds of plants
US5545545A (en) 1993-04-27 1996-08-13 Regents Of The University Of Minnesota Lysine-insensitive maize dihydrodipicolinic acid synthase
US6326527B1 (en) 1993-08-25 2001-12-04 Dekalb Genetics Corporation Method for altering the nutritional content of plant seed
CA2177351C (en) 1993-11-30 2007-04-24 Saverio Carl Falco Chimeric genes and methods for increasing the lysine content of the seeds of corn, soybean and rapeseed plants
US5578716A (en) * 1993-12-01 1996-11-26 Mcgill University DNA methyltransferase antisense oligonucleotides
US6054299A (en) * 1994-04-29 2000-04-25 Conrad; Charles A. Stem-loop cloning vector and method
US6161208A (en) * 1994-05-06 2000-12-12 International Business Machines Corporation Storage subsystem including an error correcting cache and means for performing memory to memory transfers
TR199902349T2 (xx) 1997-03-27 2000-01-21 E.I. Du Pont De Nemours And Company Kimerik genler, lizin i�eri�ini artt�rmak i�in y�ntemler
GB9710475D0 (en) 1997-05-21 1997-07-16 Zeneca Ltd Gene silencing
US20090077685A1 (en) 1998-06-16 2009-03-19 Buehler Robert E Nucleic acid molecules and other molecules associated with plants
US6506559B1 (en) * 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
ATE526406T1 (de) * 1998-03-20 2011-10-15 Commw Scient Ind Res Org Kontrolle der genexpression
CN1202246C (zh) 1998-04-08 2005-05-18 联邦科学和工业研究组织 获得修饰表型的方法和措施
US6635806B1 (en) 1998-05-14 2003-10-21 Dekalb Genetics Corporation Methods and compositions for expression of transgenes in plants
US6307123B1 (en) 1998-05-18 2001-10-23 Dekalb Genetics Corporation Methods and compositions for transgene identification
AR020078A1 (es) 1998-05-26 2002-04-10 Syngenta Participations Ag Metodo para alterar la expresion de un gen objetivo en una celula de planta
US6326193B1 (en) * 1999-11-05 2001-12-04 Cambria Biosciences, Llc Insect control agent
DE10020785A1 (de) 2000-04-28 2001-10-31 Basf Ag Polyarylethersulfon/Polyamid-Blends mit verbesserter Zähigkeit und Fließfähigkeit
BR122013026754B1 (pt) 2000-06-22 2018-02-27 Monsanto Company Molécula de dna e processos para produzir uma planta de milho tolerante à aplicação do herbicida glifosato
AU2001272977A1 (en) * 2000-06-23 2002-01-08 E.I. Du Pont De Nemours And Company Recombinant constructs and their use in reducing gene expression
US7109393B2 (en) * 2000-08-15 2006-09-19 Mendel Biotechnology, Inc. Methods of gene silencing using inverted repeat sequences
US20020048814A1 (en) * 2000-08-15 2002-04-25 Dna Plant Technology Corporation Methods of gene silencing using poly-dT sequences
US6858778B1 (en) 2000-11-07 2005-02-22 Pioneer Hi-Bred International, Inc. Plants transformed with a DNA construct comprising a nucleic acid molecule encoding an 18 kD α-globulin
AUPR210300A0 (en) * 2000-12-15 2001-01-25 Grain Biotechnology Australia Pty Ltd Infection resistant plants and methods for their generation
KR20040029122A (ko) * 2001-08-27 2004-04-03 신젠타 파티서페이션즈 아게 자가-프로세싱 식물 및 식물 부분
DE10212892A1 (de) 2002-03-20 2003-10-09 Basf Plant Science Gmbh Konstrukte und Verfahren zur Regulation der Genexpression
DE10212893A1 (de) 2002-03-20 2003-10-16 Basf Plant Science Gmbh Verfahren zum Erhöhen des Ölgehaltes in Pflanzen
EP1516051A4 (en) * 2002-06-21 2007-05-02 Monsanto Technology Llc INTRON DOUBLE-STRANDED RNA HYBRID GENES, AND USES THEREOF
US20050255568A1 (en) 2003-05-30 2005-11-17 Bailey Richard B Methods and compositions for amino acid production
US7157281B2 (en) 2003-12-11 2007-01-02 Monsanto Technology Llc High lysine maize compositions and event LY038 maize plants
US7683237B2 (en) 2004-02-10 2010-03-23 Monsanto Technology Llc Maize seed with synergistically enhanced lysine content
US20060075515A1 (en) 2004-08-11 2006-04-06 Luethy Michael H Enhanced zein reduction in transgenic corn seed
AR047598A1 (es) 2004-02-10 2006-01-25 Monsanto Technology Llc Semilla de maiz transgenica con mayor contenido de aminoacidos
US7855323B2 (en) 2004-02-10 2010-12-21 Monsanto Technology Llc Recombinant DNA for gene suppression
AU2005323166B2 (en) 2004-12-21 2011-11-10 Monsanto Technology, Llc Recombinant DNA constructs and methods for controlling gene expression
US8088976B2 (en) 2005-02-24 2012-01-03 Monsanto Technology Llc Methods for genetic control of plant pest infestation and compositions thereof
BRPI0616848A8 (pt) 2005-10-03 2017-09-19 Monsanto Technology Llc Semente de planta transgênica com maior teor de lisina

Also Published As

Publication number Publication date
BRPI0514213A (pt) 2008-06-03
EP1799833A4 (en) 2008-07-02
US20110126321A1 (en) 2011-05-26
US9006414B2 (en) 2015-04-14
US7855323B2 (en) 2010-12-21
WO2007024207A8 (en) 2008-09-25
WO2007024207A1 (en) 2007-03-01
US20150191728A1 (en) 2015-07-09
CA2583501A1 (en) 2007-03-01
US9976139B2 (en) 2018-05-22
EP1799833A1 (en) 2007-06-27
US20050176670A1 (en) 2005-08-11

Similar Documents

Publication Publication Date Title
AR050294A1 (es) Reduccion de zeina en semillas de maiz transgenico
AR047598A1 (es) Semilla de maiz transgenica con mayor contenido de aminoacidos
AR093346A2 (es) Construccion genetica que comprende una secuencia de un promotor de antiquitina de arroz y vector que la comprende
ES2556211T3 (es) Agrupación de genes novedosa
EP2489726A3 (en) Genes and uses for plant improvement
WO2006138005A3 (en) Genes and uses for plant improvement
ECSP066908A (es)
AR078696A1 (es) Polinucleotidos y polipeptidos aislados, y metodos para utilizarlos para mejorar la tolerancia al estres abiotico, rendimiento, tasa de crecimiento, vigor, biomasa, contenido de aceite y/o eficacia en el uso de nitrogeno de las plantas
EP2537937A3 (en) Genes and uses for plant enhancement
WO2008070179A3 (en) Genes and uses for plant improvement
CO6450629A2 (es) Gen de la proteína quinasa que se relaciona con snf1 de planta
AR054171A1 (es) Aumento de rendimiento en plantas con sobreexpresion de los genes accdp
AR046090A1 (es) Plantas con actividad aumentada de una enzima de ramificacion de la clase 3
AR047166A1 (es) Promotor de metalotioneina de maiz
WO2009091518A3 (en) Isolated novel nucleic acid and protein molecules from corn and methods of using those molecules to generate transgenic plant with enhanced agronomic traits
WO2007130804A3 (en) Method for producing fertile crosses between wild and domestic soybean species
EP2441841A3 (en) Increased seed size and seed number through transgenic over expression of a growth and/or development related gene during early embryo development
WO2009097133A3 (en) Transgenic plants with enhanced agronomic traits
WO2008130985A3 (en) Plants with multiple transgenes on a chromosome
AR082487A1 (es) Metodo para aumentar la resistencia a la roya de la soja en plantas transgenicas mediante el gen adr-1
Semiarti Orchid biotechnology for Indonesian orchids conservation and industry
CO6990667A2 (es) Defensa superior de soja conta plagas
De Mayolo et al. Moxalactam as a counter-selection antibiotic for Agrobacterium-mediated transformation and its positive effects on Theobroma cacao somatic embryogenesis
DE602006016756D1 (de) Auf cis einwirkende regulierende elemente aus tripsacum-dactyloiden
AR051866A1 (es) Plantas con aumento del rendimiento y metodo de prepracion

Legal Events

Date Code Title Description
FA Abandonment or withdrawal