AR043193A1 - SELECTION PROCESS OF THE MAMIFER SpermATOZOID SEX AND FROZEN SEXED SEMEN DOSE QUALITY QUALITY CONTROL METHOD - Google Patents

SELECTION PROCESS OF THE MAMIFER SpermATOZOID SEX AND FROZEN SEXED SEMEN DOSE QUALITY QUALITY CONTROL METHOD

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Publication number
AR043193A1
AR043193A1 ARP040100475A AR043193A1 AR 043193 A1 AR043193 A1 AR 043193A1 AR P040100475 A ARP040100475 A AR P040100475A AR 043193 A1 AR043193 A1 AR 043193A1
Authority
AR
Argentina
Prior art keywords
sperm
semen
sex
density gradient
sexed semen
Prior art date
Application number
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Spanish (es)
Inventor
Hossepian De Lima Vera Fernanda Martins
Di-Tanno Ramalho Mariney Flavia Pereira
Filho Carlos Alberto Moreira
Original Assignee
Fundacao De Amparo A Pesquisa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fundacao De Amparo A Pesquisa filed Critical Fundacao De Amparo A Pesquisa
Publication of AR043193A1 publication Critical patent/AR043193A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0612—Germ cells sorting of gametes, e.g. according to sex or motility

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Proceso de selección del sexo de espermatozoides mamíferos y método de control de calidad de dosis de sémen sexado congelado. Dicho proceso permite producir por centrifugación en gradiente de densidad, en empresas especializadas en la producción y comercialización de sémen congelado, dosis de sémen enriquecidas con espermatozoides portadores del cromosoma X o Y, sin comprometer la capacidad fecundativa, de estos espermatozoides. Dosis de espermatozoides que contienen ambos espermatozoides X e Y pueden ser separadas para producir subpoblaciones de espermatozoides enriquecidas con portadores del cromosoma X o Y, las cuales son sustancialmente puras con relación al espermatozoide deseado y sustancialmente libres el otro tipo de espermatozoide Reivindicación 1: Proceso de selección del sexo de espermatozoides mamíferos partiendo de la obtención del sémen in natura de reproductores según procedimientos de rutina, caracterizado por comprender la siguientes etapas: a) centrifugación en gradiente de densidad compuesto de medio de cultura químicamente definido para preservar la estabilidad e integridad de la membrana de los espermatozoides; b) utilización de temperaturas de 6 a 22sC, que preservan la viabilidad espermática; c) estabilización del pH en la banda fisiológica para cada especie, preferiblemente alrededor de 7,4; d) asociación a la presencia e sustancias que preserven la integridad espermática, utilizando preferiblemente glucosa, que evita la capacitación y reacción acrosómica precoces, especialmente en bovinos; y e) congelación de los espermatozoides luego de centrifugación en gradiente de densidad en diluidores propios para este propósito, tales como el uso de Tris-yema de huevo, para la utilización en la producción in vitro de embriones (PIV) o en la inseminación artificial (IA) convencional (nacimientos preferiblemente del sexo femenino o masculino o la realizadas después de tratamiento hormonal de las hembras para producir la sincronización del cío e/ou múltiples ovulaciones, cosecha de los embriones ( en su mayoría del sexo femenino o masculino) y posterior transferencia para hembras receptoras. Reivindicación 9: Sémen sexado según cualesquiera de las reivindicaciones anteriores, caracterizado por contener subpoblaciones de espermatozoides enriquecidas con portadores del cromosoma X o Y y ser sometido a las siguientes pruebas: control de calidad e las dosis de sémen que contienen espermatozoides centrifugados en gradiente de densidad, neque dicho protocolo de control de la calidad de fertilidad de los espermatozoides sometidos a sexaje es compuesto por: a) evaluación de motilidad y vigor y prueba de término resistencia (TTR); b) coloración específica para evaluar la integridad de la membrana de los espermatozoides; c) producción in vitro de embriones ; y d) inseminación artificial a través del método comercial. Reivindicación 10: Método de inseminación artificial, caracterizado por utilizar el sémen sexado según la reivindicación 9. Reivindicación 11: Método de producción in vitro de embriones, caracterizado por utilizar sémen sexado según la reivindicación 9.Selection process of mammalian sperm sex and method of dose quality control of frozen sexed semen. This process allows to produce by centrifugation in density gradient, in companies specialized in the production and commercialization of frozen semen, doses of semen enriched with sperm carrying the X or Y chromosome, without compromising the fertilization capacity of these sperm. Dose of sperm containing both X and Y sperm can be separated to produce subpopulations of sperm enriched with carriers of the X or Y chromosome, which are substantially pure relative to the desired sperm and substantially free of the other type of sperm Claim 1: Process of Selection of the sex of mammalian sperm based on the obtaining of the semen in natura from breeders according to routine procedures, characterized by comprising the following stages: a) density gradient centrifugation composed of chemically defined culture medium to preserve the stability and integrity of the sperm membrane; b) use of temperatures from 6 to 22sC, which preserve sperm viability; c) pH stabilization in the physiological band for each species, preferably around 7.4; d) association with the presence and substances that preserve sperm integrity, preferably using glucose, which prevents early training and acrosomic reaction, especially in cattle; and e) freezing of sperm after density gradient centrifugation in own diluents for this purpose, such as the use of egg Tris-yolk, for use in the in vitro production of embryos (PIV) or artificial insemination ( AI) Conventional (preferably female or male births or those made after hormonal treatment of females to produce synchronization of the spine and / or multiple ovulations, embryo harvest (mostly female or male) and subsequent transfer for recipient females Claim 9: Sexed semen according to any of the preceding claims, characterized by containing subpopulations of sperm enriched with carriers of the X or Y chromosome and being subjected to the following tests: quality control and semen doses containing centrifuged sperm in density gradient, neque said control protocol of the fertility quality of sperm subjected to sexing is composed of: a) evaluation of motility and vigor and endurance resistance test (TTR); b) specific coloration to assess the integrity of the sperm membrane; c) in vitro production of embryos; and d) artificial insemination through the commercial method. Claim 10: Artificial insemination method, characterized by using the sexed semen according to claim 9. Claim 11: Method of in vitro production of embryos, characterized by using sexed semen according to claim 9.

ARP040100475 2003-02-17 2004-02-17 SELECTION PROCESS OF THE MAMIFER SpermATOZOID SEX AND FROZEN SEXED SEMEN DOSE QUALITY QUALITY CONTROL METHOD AR043193A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
BR0300604A BR0300604A (en) 2003-02-17 2003-02-17 Sex selection process of mammalian sperm and quality control method of frozen sexed semen doses

Publications (1)

Publication Number Publication Date
AR043193A1 true AR043193A1 (en) 2005-07-20

Family

ID=32854956

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP040100475 AR043193A1 (en) 2003-02-17 2004-02-17 SELECTION PROCESS OF THE MAMIFER SpermATOZOID SEX AND FROZEN SEXED SEMEN DOSE QUALITY QUALITY CONTROL METHOD

Country Status (3)

Country Link
AR (1) AR043193A1 (en)
BR (1) BR0300604A (en)
WO (1) WO2004072220A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050114915A1 (en) * 2003-11-26 2005-05-26 Vicam L.P. Method for sex biasing spermatozoa
US9157063B2 (en) 2007-01-16 2015-10-13 Texas Tech University System Method and apparatus for gender selection based on pH
ITTO20100651A1 (en) * 2010-07-28 2012-01-29 Ist Sperimentale It Lazzaro Spallanza PROCEDURE AND EQUIPMENT FOR THE CHARACTERIZATION AND SEPARATION OF SPERMFERCEE WITH SUSPENDED LEVER MICROMETRIC SENSORS
CN104962513A (en) * 2015-06-30 2015-10-07 阜阳师范学院 Fox X sperm separating method
EP3775881B1 (en) * 2018-03-30 2023-05-03 Texas Tech University System System and method for non-invasive embryo sexing
CN109370981A (en) * 2018-11-07 2019-02-22 临泉县欣达生态羊业有限公司 The X Gender preselection of Anhui White Goats
CN109355250A (en) * 2018-11-07 2019-02-19 临泉县欣达生态羊业有限公司 The y sperm separation method of Anhui White Goats

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3894529A (en) * 1969-04-10 1975-07-15 Bio Controls Inc Method and means for controlling the sex of mammalian offspring and product therefor
SE0004777D0 (en) * 2000-12-22 2000-12-22 Amersham Pharm Biotech Ab Separation of X and Y sperm cells

Also Published As

Publication number Publication date
WO2004072220A3 (en) 2004-10-07
BR0300604A (en) 2004-11-03
WO2004072220A2 (en) 2004-08-26

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