AR032921A1 - Concentracion y lisis de celulas infectadas por adenovirus en una unica operacion unitaria - Google Patents

Concentracion y lisis de celulas infectadas por adenovirus en una unica operacion unitaria

Info

Publication number
AR032921A1
AR032921A1 ARP020100733A ARP020100733A AR032921A1 AR 032921 A1 AR032921 A1 AR 032921A1 AR P020100733 A ARP020100733 A AR P020100733A AR P020100733 A ARP020100733 A AR P020100733A AR 032921 A1 AR032921 A1 AR 032921A1
Authority
AR
Argentina
Prior art keywords
cells
adenovirus
lisis
concentration
single unit
Prior art date
Application number
ARP020100733A
Other languages
English (en)
Original Assignee
Schering Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Schering Ag filed Critical Schering Ag
Publication of AR032921A1 publication Critical patent/AR032921A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/066—Lysis of microorganisms by physical methods
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10351—Methods of production or purification of viral material

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Un método para preparar entidades biologicas intracelulares (por ejemplo, organismos, tales como, por ejemplo, virus, particularmente Adenovirus; organelas o moléculas biologicas), que comprende someter las células que contienen entidades biologicas a una centrifugacion contínua, bajo condiciones efectivas para concentrar las células en un precipitado celular; y eyectar las células precipitadas de la centrífuga en un receptáculo de recoleccion, bajo condiciones efectivas para lisar las células; donde se realizan pasos efectivos adicionales para lograr la lisis celular.
ARP020100733A 2001-03-01 2002-02-28 Concentracion y lisis de celulas infectadas por adenovirus en una unica operacion unitaria AR032921A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US27203401P 2001-03-01 2001-03-01

Publications (1)

Publication Number Publication Date
AR032921A1 true AR032921A1 (es) 2003-12-03

Family

ID=23038121

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP020100733A AR032921A1 (es) 2001-03-01 2002-02-28 Concentracion y lisis de celulas infectadas por adenovirus en una unica operacion unitaria

Country Status (10)

Country Link
US (1) US6773909B2 (es)
EP (1) EP1363997B1 (es)
JP (2) JP4559026B2 (es)
AR (1) AR032921A1 (es)
AT (1) ATE496120T1 (es)
DE (1) DE60238970D1 (es)
ES (1) ES2355065T3 (es)
PE (1) PE20020960A1 (es)
UY (1) UY27191A1 (es)
WO (1) WO2002070673A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070207461A1 (en) 2004-02-23 2007-09-06 Crucell Holland B.V. Virus Purification Methods
US7592139B2 (en) * 2004-09-24 2009-09-22 Sandia National Laboratories High temperature flow-through device for rapid solubilization and analysis
US8574595B2 (en) 2005-04-11 2013-11-05 Crucell Holland B.V. Virus purification using ultrafiltration
US20100216657A1 (en) * 2006-05-16 2010-08-26 Arcxis Biotechnologies, Inc. Pcr-free sample preparation and detection systems for high speed biologic analysis and identification
US20080194006A1 (en) * 2007-02-08 2008-08-14 Embrex, Inc. Methods of releasing sporocysts from oocysts using controlled shear forces
CN114176049B (zh) * 2022-01-20 2023-01-31 河南农业大学 一种同时提高室内繁育棉铃虫齿唇姬蜂出蜂率和雌虫数量的方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5721120A (en) * 1996-10-02 1998-02-24 Merck & Co., Inc. Method of disrupting cultured cells using an impinging jet device

Also Published As

Publication number Publication date
DE60238970D1 (de) 2011-03-03
ATE496120T1 (de) 2011-02-15
JP4559026B2 (ja) 2010-10-06
EP1363997B1 (en) 2011-01-19
JP2009189370A (ja) 2009-08-27
PE20020960A1 (es) 2002-11-14
ES2355065T3 (es) 2011-03-22
EP1363997A1 (en) 2003-11-26
UY27191A1 (es) 2002-10-31
US20030049829A1 (en) 2003-03-13
JP2004533814A (ja) 2004-11-11
WO2002070673A1 (en) 2002-09-12
US6773909B2 (en) 2004-08-10

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